ATE233325T1 - Fluoreszenz-basierte hts-(high throughput screening)assayverfahren für proteinkinase und phosphatase - Google Patents

Fluoreszenz-basierte hts-(high throughput screening)assayverfahren für proteinkinase und phosphatase

Info

Publication number
ATE233325T1
ATE233325T1 AT98961797T AT98961797T ATE233325T1 AT E233325 T1 ATE233325 T1 AT E233325T1 AT 98961797 T AT98961797 T AT 98961797T AT 98961797 T AT98961797 T AT 98961797T AT E233325 T1 ATE233325 T1 AT E233325T1
Authority
AT
Austria
Prior art keywords
phosphatase
high throughput
throughput screening
fluorescence
protein kinase
Prior art date
Application number
AT98961797T
Other languages
English (en)
Inventor
Dennis E Epps
Charles K Marschke
Original Assignee
Upjohn Co
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Upjohn Co filed Critical Upjohn Co
Application granted granted Critical
Publication of ATE233325T1 publication Critical patent/ATE233325T1/de

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6842—Proteomic analysis of subsets of protein mixtures with reduced complexity, e.g. membrane proteins, phosphoproteins, organelle proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • C12Q1/42—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving phosphatase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/48—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving transferase
    • C12Q1/485—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving transferase involving kinase
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/573—Immunoassay; Biospecific binding assay; Materials therefor for enzymes or isoenzymes
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/90—Enzymes; Proenzymes
    • G01N2333/91—Transferases (2.)
    • G01N2333/912—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • G01N2333/91205—Phosphotransferases in general
    • G01N2333/9121—Phosphotransferases in general with an alcohol group as acceptor (2.7.1), e.g. general tyrosine, serine or threonine kinases
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/90—Enzymes; Proenzymes
    • G01N2333/914—Hydrolases (3)
    • G01N2333/916—Hydrolases (3) acting on ester bonds (3.1), e.g. phosphatases (3.1.3), phospholipases C or phospholipases D (3.1.4)
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/968—High energy substrates, e.g. fluorescent, chemiluminescent, radioactive
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S436/00—Chemistry: analytical and immunological testing
    • Y10S436/80—Fluorescent dyes, e.g. rhodamine

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Hematology (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Urology & Nephrology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Analytical Chemistry (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Biophysics (AREA)
  • Pathology (AREA)
  • General Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Food Science & Technology (AREA)
  • Cell Biology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
  • Peptides Or Proteins (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
AT98961797T 1997-12-05 1998-12-02 Fluoreszenz-basierte hts-(high throughput screening)assayverfahren für proteinkinase und phosphatase ATE233325T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US6783397P 1997-12-05 1997-12-05
PCT/US1998/025196 WO1999029894A1 (en) 1997-12-05 1998-12-02 Fluorescence-based high throughput screening assays for protein kinases and phosphatases

Publications (1)

Publication Number Publication Date
ATE233325T1 true ATE233325T1 (de) 2003-03-15

Family

ID=22078714

Family Applications (1)

Application Number Title Priority Date Filing Date
AT98961797T ATE233325T1 (de) 1997-12-05 1998-12-02 Fluoreszenz-basierte hts-(high throughput screening)assayverfahren für proteinkinase und phosphatase

Country Status (11)

Country Link
US (3) US6203994B1 (de)
EP (1) EP1036192B1 (de)
JP (1) JP2001526380A (de)
AT (1) ATE233325T1 (de)
AU (1) AU738194B2 (de)
CA (1) CA2306686A1 (de)
DE (1) DE69811724T2 (de)
DK (1) DK1036192T3 (de)
ES (1) ES2192801T3 (de)
PT (1) PT1036192E (de)
WO (1) WO1999029894A1 (de)

Families Citing this family (75)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0938586A4 (de) * 1996-10-28 2002-01-30 Panvera Corp Messung der kinaseaktivität mittels fluoreszentpolarisation
US20030036106A1 (en) * 1996-10-28 2003-02-20 Burke Thomas J. Kinase and phosphatase activity measurement using fluorescence polarization
US20030027236A1 (en) * 1996-10-28 2003-02-06 Burke Thomas J. Kinase activity measurement using flourescence polarization
US7070921B2 (en) 2000-04-28 2006-07-04 Molecular Devices Corporation Molecular modification assays
US7632651B2 (en) 1997-09-15 2009-12-15 Mds Analytical Technologies (Us) Inc. Molecular modification assays
FR2768817B1 (fr) * 1997-09-19 1999-12-10 Cis Bio Int Methode homogene pour la detection et/ou la determination de l'activite phosphorylante d'un materiel biologique
US6982431B2 (en) 1998-08-31 2006-01-03 Molecular Devices Corporation Sample analysis systems
US6498005B1 (en) * 1998-09-30 2002-12-24 Caliper Technologies Corp. Homogeneous assay methods
US20020192719A1 (en) * 1998-09-30 2002-12-19 Caliper Technologies Corp. Homogeneous assay methods
US6821738B2 (en) * 1999-01-20 2004-11-23 The Board Of Regents For Oklahoma State University Broad spectrum bio-detection of nerve agents, organophosphates, and other chemical warfare agents
US6972198B2 (en) * 1999-02-26 2005-12-06 Cyclacel, Ltd. Methods and compositions using protein binding partners
US7166475B2 (en) * 1999-02-26 2007-01-23 Cyclacel Ltd. Compositions and methods for monitoring the modification state of a pair of polypeptides
US6472141B2 (en) 1999-05-21 2002-10-29 Caliper Technologies Corp. Kinase assays using polycations
US6287774B1 (en) * 1999-05-21 2001-09-11 Caliper Technologies Corp. Assay methods and system
EP1081232A1 (de) * 1999-08-30 2001-03-07 K.U. Leuven Research & Development Neues Ziel für Fungizide
AU7511800A (en) * 1999-08-30 2001-03-26 K.U. Leuven Research And Development Novel target for antiparasitic agents and inhibitors thereof
US6670167B1 (en) 1999-11-01 2003-12-30 Agouron Pharmaceuticals, Inc. Catalytic domain of the human effector cell cycle checkpoint protein kinase materials and methods for identification of inhibitors thereof
US6406869B1 (en) * 1999-10-22 2002-06-18 Pharmacopeia, Inc. Fluorescent capture assay for kinase activity employing anti-phosphotyrosine antibodies as capture and detection agents
US6582907B1 (en) * 1999-12-09 2003-06-24 Pharmacia & Upjohn Company Use of fluorescence correlation spectroscopy to identify compounds that bind to target species under isothermal denaturing conditions
US6376180B1 (en) 1999-12-09 2002-04-23 Pharmacia & Upjohn Company Methods of identifying compounds that bind to target species under isothermal denaturing conditions
US20060040319A1 (en) * 2000-01-14 2006-02-23 Tom Muir Multiple sensor-containing active modified polypeptides, preparation and uses thereof
WO2001061031A2 (en) * 2000-02-14 2001-08-23 Ceptyr, Inc. Improved assay for protein tyrosine phosphatases
US6365366B1 (en) * 2000-03-13 2002-04-02 Tularik Inc. T2k kinase assays
GB0007915D0 (en) * 2000-03-31 2000-05-17 Univ Bristol Protein kinase assay
GB0011439D0 (en) * 2000-05-12 2000-06-28 Novartis Res Found Cancer diagnosis and assays for screening
US7049080B2 (en) 2000-08-11 2006-05-23 Joachim Kramer Process for detecting serine/threonine kinase activity
EP1182263A1 (de) * 2000-08-11 2002-02-27 Evotec OAI AG Verfahren zum Nachweis einer Threonin- oder Serinkinasenaktivität
WO2002014543A2 (en) * 2000-08-11 2002-02-21 Evotec Oai Ag Process for detecting enzyme activity in an immunoassay
EP1199370B1 (de) * 2000-10-18 2004-04-21 Evotec OAI AG Verfahren zur Auffindung von Dephosphorylierung phosphorylierte Threonin oder Serin durch Phosphataseaktivität
EP1217078A1 (de) * 2000-12-21 2002-06-26 Evotec OAI AG Phosphatase-Immunoassay mit Antikörper gegen bisphosphorylierte Substrate
US20030162230A1 (en) 2000-09-27 2003-08-28 Reagan Kevin J. Method for quantifying phosphokinase activity on proteins
ATE378400T1 (de) * 2001-02-14 2007-11-15 Sysmex Corp Verfahren zur bestimmung der aktivität des zellzyklus-regulationsfaktors und verfahren zur diagnose von krebs unter verwendung desselben
WO2002077623A1 (fr) * 2001-03-23 2002-10-03 Japan Science And Technology Corporation Sonde pour visualiser la phosphorylation/dephosphorylation de proteines et procede de detection et de quantification de la phosphorylation/dephosphorylation de proteines
EP1264897A3 (de) * 2001-06-06 2003-11-12 Europäisches Laboratorium Für Molekularbiologie (Embl) Synthetisches Sensorpeptid für Kinase oder Phosphatase Assays
AU2002363530A1 (en) * 2001-11-07 2003-05-19 Sensor Technologies Llc Method of identifying energy transfer sensors for analytes
US7067269B2 (en) * 2001-11-26 2006-06-27 Echelon Biosciences, Inc. Assaying apparatus, kit, and method for lipids and associated enzymes
GB0208987D0 (en) * 2002-04-19 2002-05-29 Amersham Biosciences Uk Ltd Methods for measuring protein kinase and phosphatase activity
US6942987B2 (en) 2002-05-15 2005-09-13 Pharmacopeia Drug Discovery, Inc. Methods for measuring kinase activity
US7195884B2 (en) * 2002-07-19 2007-03-27 Promega Corp. Methods and kits for transferases
WO2004013610A2 (en) * 2002-08-01 2004-02-12 Sensor Technologies Llc Method of measuring molecular interactions
DE10239005A1 (de) * 2002-08-26 2004-03-11 Bayer Ag Homogene Fluoreszen-Assay-Methode für Kinasen, Phosphatasen und Phosphodiesterasen
AU2003293303A1 (en) * 2002-09-11 2004-05-04 Novartis Ag Screening assay for identifying agents that modulate pak kinase or cd45 tyrosine phosphatase activity
TW200418829A (en) 2003-02-14 2004-10-01 Avanir Pharmaceutics Inhibitors of macrophage migration inhibitory factor and methods for identifying the same
US20060211063A1 (en) * 2003-03-12 2006-09-21 Assay Designs Inc. Phosphokinase assay
DE10323081A1 (de) * 2003-05-22 2004-12-16 Aventis Pharma Deutschland Gmbh Verwendung eines Polypeptides
CA2445420A1 (en) * 2003-07-29 2005-01-29 Invitrogen Corporation Kinase and phosphatase assays
US7727752B2 (en) * 2003-07-29 2010-06-01 Life Technologies Corporation Kinase and phosphatase assays
US7619059B2 (en) * 2003-07-29 2009-11-17 Life Technologies Corporation Bimolecular optical probes
JP2007503388A (ja) 2003-08-22 2007-02-22 アバニール・ファーマシューティカルズ マクロファージ遊走阻止因子の阻害剤としての置換ナフチリジン誘導体、およびヒト疾患の治療におけるそれらの使用
US7279286B2 (en) * 2003-09-11 2007-10-09 Sanofi-Aventis Deutschland Gmbh High-throughput-assay with high sensitivity for measuring of the activity of β-adrenergic receptor kinase and for determining the impact of test substances on such activity
DE602004025592D1 (de) * 2003-09-12 2010-04-01 Life Technologies Corp Anwendungen des Resonanzenergietransfers zwischen Terbium und GFP
EP1704257A2 (de) 2004-01-16 2006-09-27 Applera Corporation Fluorogene kinase-assays sowie substrate für kinasen und phosphatasen
CN101048898B (zh) 2004-10-29 2012-02-01 麦德托尼克公司 锂离子电池及医疗装置
US7662509B2 (en) * 2004-10-29 2010-02-16 Medtronic, Inc. Lithium-ion battery
US7641992B2 (en) * 2004-10-29 2010-01-05 Medtronic, Inc. Medical device having lithium-ion battery
US7582387B2 (en) 2004-10-29 2009-09-01 Medtronic, Inc. Lithium-ion battery
US7927742B2 (en) 2004-10-29 2011-04-19 Medtronic, Inc. Negative-limited lithium-ion battery
US9077022B2 (en) 2004-10-29 2015-07-07 Medtronic, Inc. Lithium-ion battery
US7879495B2 (en) * 2004-10-29 2011-02-01 Medtronic, Inc. Medical device having lithium-ion battery
US7807299B2 (en) * 2004-10-29 2010-10-05 Medtronic, Inc. Lithium-ion battery
CN101048897B (zh) * 2004-10-29 2010-10-27 麦德托尼克公司 锂离子电池充电方法
US8105714B2 (en) * 2004-10-29 2012-01-31 Medtronic, Inc. Lithium-ion battery
US7682745B2 (en) * 2004-10-29 2010-03-23 Medtronic, Inc. Medical device having lithium-ion battery
US9065145B2 (en) * 2004-10-29 2015-06-23 Medtronic, Inc. Lithium-ion battery
US8980453B2 (en) 2008-04-30 2015-03-17 Medtronic, Inc. Formation process for lithium-ion batteries
WO2006102191A1 (en) 2005-03-24 2006-09-28 Avanir Pharmaceuticals Thienopyridinone derivatives as macrophage migration inhibitory factor inhibitors
WO2007016975A1 (en) * 2005-07-29 2007-02-15 F. Hoffmann-La Roche Ag Kinase and phosphatase assays based on fret
EP2066812A4 (de) * 2006-09-21 2010-04-28 Univ Rochester Zusammensetzungen und verfahren in zusammenhang mit proteinverschiebungstherapie für myotone dystrophie
GB0624689D0 (en) * 2006-12-11 2007-01-17 Glaxo Group Ltd Novel fluorescent kinase ligands and methods employing the same
EP1933151A1 (de) 2006-12-15 2008-06-18 F. Hoffmann-La Roche Ag Prüfungen zum Messen der Phosphatmodifikationsenzymaktivität
US9287580B2 (en) 2011-07-27 2016-03-15 Medtronic, Inc. Battery with auxiliary electrode
US20130149560A1 (en) 2011-12-09 2013-06-13 Medtronic, Inc. Auxiliary electrode for lithium-ion battery
DE102014203266B4 (de) 2014-02-24 2018-07-19 Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e.V. Zusammensetzung mit FRET-Paar in definierter Geometrie
JP2018070587A (ja) * 2016-10-26 2018-05-10 国立大学法人九州大学 細胞の標識方法
WO2018079859A1 (ja) * 2016-10-26 2018-05-03 国立大学法人九州大学 細胞の標識方法

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4681859A (en) 1984-09-21 1987-07-21 Ortho Diagnostic Systems Inc. Fluorescence polarization immunoassay for heavy antigens
DE3806430A1 (de) 1988-02-29 1989-09-07 Boehringer Mannheim Gmbh Verfahren zur bestimmung eines proteins nach dem prinzip des fluoreszenz-polarisations-immunoassays
WO1993003377A1 (en) * 1991-07-31 1993-02-18 Ziltener Hermann J Method for the detection of phosphotyrosine residues
US5532167A (en) 1994-01-07 1996-07-02 Beth Israel Hospital Substrate specificity of protein kinases
CA2253776A1 (en) * 1996-05-07 1997-11-13 Boehringer Ingelheim Pharmaceuticals, Inc. Phosphotyrosine mimics and methods for identifying and using same
US5912137A (en) * 1996-07-16 1999-06-15 The Regents Of The University Of California Assays for protein kinases using fluorescent
US5759787A (en) * 1996-08-26 1998-06-02 Tularik Inc. Kinase assay
EP0938586A4 (de) * 1996-10-28 2002-01-30 Panvera Corp Messung der kinaseaktivität mittels fluoreszentpolarisation

Also Published As

Publication number Publication date
DE69811724D1 (en) 2003-04-03
CA2306686A1 (en) 1999-06-17
DK1036192T3 (da) 2003-06-10
EP1036192A1 (de) 2000-09-20
JP2001526380A (ja) 2001-12-18
PT1036192E (pt) 2003-06-30
US6203994B1 (en) 2001-03-20
AU738194B2 (en) 2001-09-13
ES2192801T3 (es) 2003-10-16
EP1036192B1 (de) 2003-02-26
US20030235865A1 (en) 2003-12-25
DE69811724T2 (de) 2003-10-16
US6630311B2 (en) 2003-10-07
US20010044117A1 (en) 2001-11-22
WO1999029894A1 (en) 1999-06-17
US6818413B2 (en) 2004-11-16
AU1703499A (en) 1999-06-28

Similar Documents

Publication Publication Date Title
DE69811724D1 (en) Fluoreszenz-basierte hts-(high throughput screening)assayverfahren für proteinkinase und phosphatase
Parker et al. Development of high throughput screening assays using fluorescence polarization: nuclear receptor-ligand–binding and kinase/phosphatase assays
DE69825066D1 (de) Veränderte proteinkinasen, die modifizierte nukleotidtriphosphat substrate verwenden können
DK0518557T3 (da) Analysefremgangsmåde for hydrolytiske enzymer
ATE85434T1 (de) Verfahren zur immunoassay.
WO2001061031A3 (en) Improved assay for protein tyrosine phosphatases
ES2196010T3 (es) Spbfamilia ptp-d de proteinas tirosina fosfatasas.
ATE145672T1 (de) Neue inhibitoren für hydrolytische enzyme und substrate und bestimmungsverfahren, selbige einschliessende verfahren und testsätze
Nakamura et al. Detection and characterization of protein kinases in rice phloem sap
WO2014165007A1 (en) Luminescent detection of inorganic phosphate and coupled reactions
Henderson et al. Substrate-selective positive allosteric modulation of PTPRD’s phosphatase by flavonols
Paudel et al. The ATPase activity of phosphorylase kinase is regulated in parallel with its protein kinase activity.
WO2002014542A3 (en) Process for detecting serine/threonine kinase activity
GB2387905B (en) Methods for measuring protein kinase and phosphatase activity
WO2004013630A1 (en) Method for determination of thymidine kinase 1 activity and the use thereof
Ogueta et al. Stage‐Specific Substrate Phosphorylation by a Ca2+/Calmodulin‐Dependent Protein Kinase in Trypanosoma cruzi
de la Houssaye et al. Activation of a cyclic AMP-independent protein kinase by an endogenous ATP-requiring protease from lymphosarcoma cells.
Reinhardt et al. Kinetic mechanism of choline kinase from rat striata.
Fialho et al. Protein phosphorylation during Rhodnius prolixus embryogenesis: protein kinase casein kinase II activity
Zhu et al. Design and synthesis of fluorogenic substrates that target protein phosphatases
DE60009891T2 (de) Assay für nukleosid-diphosphate und triphosphate
KANSRA et al. Activation of mitogen activated protein kinase in human platelets by genistein
JP2002543798A5 (de)
ATE105415T1 (de) Simultanbestimmung von calcium und phosphor.
Liu et al. Two glycosidases as label enzymes for concurrent enzyme-linked-immunosorbent-assay of two components via spectrophotometric-dual-enzyme-simultaneous-assay in one solution

Legal Events

Date Code Title Description
UEP Publication of translation of european patent specification

Ref document number: 1036192

Country of ref document: EP

REN Ceased due to non-payment of the annual fee