ATE236402T1 - Vorgefärbte 3,3',5,5'-tetramethylbenzidin- substrate zum nachweis von enzymaktivität - Google Patents
Vorgefärbte 3,3',5,5'-tetramethylbenzidin- substrate zum nachweis von enzymaktivitätInfo
- Publication number
- ATE236402T1 ATE236402T1 AT98933576T AT98933576T ATE236402T1 AT E236402 T1 ATE236402 T1 AT E236402T1 AT 98933576 T AT98933576 T AT 98933576T AT 98933576 T AT98933576 T AT 98933576T AT E236402 T1 ATE236402 T1 AT E236402T1
- Authority
- AT
- Austria
- Prior art keywords
- substrates
- substrate
- tmb
- enzyme
- tetramethylbenzidine
- Prior art date
Links
- 239000000758 substrate Substances 0.000 title abstract 10
- UAIUNKRWKOVEES-UHFFFAOYSA-N 3,3',5,5'-tetramethylbenzidine Chemical compound CC1=C(N)C(C)=CC(C=2C=C(C)C(N)=C(C)C=2)=C1 UAIUNKRWKOVEES-UHFFFAOYSA-N 0.000 title abstract 3
- 239000000975 dye Substances 0.000 abstract 3
- 238000002965 ELISA Methods 0.000 abstract 2
- MHAJPDPJQMAIIY-UHFFFAOYSA-N Hydrogen peroxide Chemical compound OO MHAJPDPJQMAIIY-UHFFFAOYSA-N 0.000 abstract 2
- 238000002835 absorbance Methods 0.000 abstract 2
- 150000002978 peroxides Chemical class 0.000 abstract 2
- 240000003291 Armoracia rusticana Species 0.000 abstract 1
- 235000011330 Armoracia rusticana Nutrition 0.000 abstract 1
- 102000004190 Enzymes Human genes 0.000 abstract 1
- 108090000790 Enzymes Proteins 0.000 abstract 1
- 239000004372 Polyvinyl alcohol Substances 0.000 abstract 1
- CBMCZKMIOZYAHS-NSCUHMNNSA-N [(e)-prop-1-enyl]boronic acid Chemical compound C\C=C\B(O)O CBMCZKMIOZYAHS-NSCUHMNNSA-N 0.000 abstract 1
- 239000003795 chemical substances by application Substances 0.000 abstract 1
- 238000001952 enzyme assay Methods 0.000 abstract 1
- ZBQZBWKNGDEDOA-UHFFFAOYSA-N eosin B Chemical compound O1C(=O)C2=CC=CC=C2C21C1=CC([N+]([O-])=O)=C(O)C(Br)=C1OC1=C2C=C([N+]([O-])=O)C(O)=C1Br ZBQZBWKNGDEDOA-UHFFFAOYSA-N 0.000 abstract 1
- 238000000034 method Methods 0.000 abstract 1
- VIKNJXKGJWUCNN-XGXHKTLJSA-N norethisterone Chemical compound O=C1CC[C@@H]2[C@H]3CC[C@](C)([C@](CC4)(O)C#C)[C@@H]4[C@@H]3CCC2=C1 VIKNJXKGJWUCNN-XGXHKTLJSA-N 0.000 abstract 1
- 239000003960 organic solvent Substances 0.000 abstract 1
- 235000019422 polyvinyl alcohol Nutrition 0.000 abstract 1
- 229920002451 polyvinyl alcohol Polymers 0.000 abstract 1
- AQLJVWUFPCUVLO-UHFFFAOYSA-N urea hydrogen peroxide Chemical compound OO.NC(N)=O AQLJVWUFPCUVLO-UHFFFAOYSA-N 0.000 abstract 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 abstract 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
- G01N33/581—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with enzyme label (including co-enzymes, co-factors, enzyme inhibitors or substrates)
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/531—Production of immunochemical test materials
- G01N33/532—Production of labelled immunochemicals
- G01N33/535—Production of labelled immunochemicals with enzyme label or co-enzymes, co-factors, enzyme inhibitors or enzyme substrates
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S435/00—Chemistry: molecular biology and microbiology
- Y10S435/968—High energy substrates, e.g. fluorescent, chemiluminescent, radioactive
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Immunology (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Chemical & Material Sciences (AREA)
- Hematology (AREA)
- Urology & Nephrology (AREA)
- Food Science & Technology (AREA)
- Biochemistry (AREA)
- Cell Biology (AREA)
- Biotechnology (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Microbiology (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Plural Heterocyclic Compounds (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DK87097 | 1997-07-15 | ||
| PCT/DK1998/000333 WO1999004261A1 (en) | 1997-07-15 | 1998-07-15 | Pre-stained 3,3',5,5'-tetramethylbenzidine substrates for the detection of enzyme activity |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ATE236402T1 true ATE236402T1 (de) | 2003-04-15 |
Family
ID=8098463
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AT98933576T ATE236402T1 (de) | 1997-07-15 | 1998-07-15 | Vorgefärbte 3,3',5,5'-tetramethylbenzidin- substrate zum nachweis von enzymaktivität |
Country Status (6)
| Country | Link |
|---|---|
| US (1) | US6221624B1 (de) |
| EP (1) | EP1000358B1 (de) |
| AT (1) | ATE236402T1 (de) |
| AU (1) | AU8334698A (de) |
| DE (1) | DE69812966T2 (de) |
| WO (1) | WO1999004261A1 (de) |
Families Citing this family (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| ATE236402T1 (de) * | 1997-07-15 | 2003-04-15 | Kem En Tec As | Vorgefärbte 3,3',5,5'-tetramethylbenzidin- substrate zum nachweis von enzymaktivität |
| US20040219620A1 (en) * | 2003-04-29 | 2004-11-04 | Mayer Brent A. | Chromogenic substrate with a pH indicator dye |
| RU2268253C1 (ru) * | 2004-09-28 | 2006-01-20 | Наталья Александровна Шамарова | Композиция для хранения водного раствора 3,3',5,5'-тетраметилбензидина гидрохлорида (тмб) и пероксида мочевины |
| CN103698505B (zh) * | 2013-12-13 | 2015-06-10 | 山东博科生物产业有限公司 | 一种高效稳定的tmb显色液及其制备方法 |
| US10408825B2 (en) * | 2015-05-19 | 2019-09-10 | Electronics And Telecommunications Research Institute | Biosensor |
| CN105116141B (zh) * | 2015-07-02 | 2016-09-14 | 深圳市卫光生物制品股份有限公司 | 一种单组份tmb显色液及其制备方法 |
Family Cites Families (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE1952716U (de) | 1966-10-05 | 1966-12-29 | Richard Schwarz | Getraenkeflasche. |
| US4128629A (en) * | 1977-07-05 | 1978-12-05 | Corning Glass Works | Extraction-free cortisol assay |
| US4503143A (en) | 1982-08-20 | 1985-03-05 | Btc Diagnostics Limited Partnership | Enzyme immunoassay with two-part solution of tetramethylbenzidine as chromogen |
| US4596770A (en) | 1984-06-01 | 1986-06-24 | Travenol-Genetech Diagnostics | Assay of peroxidase enzyme activity |
| GB8505899D0 (en) | 1985-03-07 | 1985-04-11 | Boots Celltech Diagnostics | Assay reagents |
| DE3541979A1 (de) | 1985-11-28 | 1987-06-04 | Behringwerke Ag | Mittel zur bestimmung von peroxidase-aktivitaet mit stabilisator, verfahren zur herstellung und seine verwendung |
| EP0279614A3 (de) | 1987-02-17 | 1990-03-21 | Synbiotics Corporation | Stabile Formulierung eines Peroxidsubstrats |
| JP2573009B2 (ja) | 1988-01-20 | 1997-01-16 | コニカ株式会社 | 基質試薬液の安定化方法 |
| US5013646A (en) | 1989-11-03 | 1991-05-07 | Transgenic Sciences, Inc. | TMB Formulation for soluble and precipitable HRP-ELISA |
| US5318894A (en) | 1990-01-30 | 1994-06-07 | Miles Inc. | Composition, device and method of assaying for peroxidatively active substances |
| WO1992015883A1 (en) | 1991-03-08 | 1992-09-17 | Amoco Corporation | Improved assays including colored organic compounds |
| DE19527160C1 (de) | 1995-07-25 | 1997-01-23 | Brahms Diagnostica Gmbh | Verfahren zur Messung der Konzentration eines Enzyms durch photometrische Absorptionsmessung eines enzymatisch gebildeten Farbstoffs in einer Meßlösung |
| ATE236402T1 (de) * | 1997-07-15 | 2003-04-15 | Kem En Tec As | Vorgefärbte 3,3',5,5'-tetramethylbenzidin- substrate zum nachweis von enzymaktivität |
| GB0229618D0 (en) | 2002-12-19 | 2003-01-22 | Cancer Rec Tech Ltd | Pyrazole compounds |
-
1998
- 1998-07-15 AT AT98933576T patent/ATE236402T1/de not_active IP Right Cessation
- 1998-07-15 EP EP98933576A patent/EP1000358B1/de not_active Expired - Lifetime
- 1998-07-15 AU AU83346/98A patent/AU8334698A/en not_active Abandoned
- 1998-07-15 DE DE69812966T patent/DE69812966T2/de not_active Expired - Lifetime
- 1998-07-15 US US09/445,802 patent/US6221624B1/en not_active Expired - Lifetime
- 1998-07-15 WO PCT/DK1998/000333 patent/WO1999004261A1/en not_active Ceased
Also Published As
| Publication number | Publication date |
|---|---|
| DE69812966D1 (de) | 2003-05-08 |
| WO1999004261A1 (en) | 1999-01-28 |
| DE69812966T2 (de) | 2004-03-04 |
| EP1000358A1 (de) | 2000-05-17 |
| US6221624B1 (en) | 2001-04-24 |
| AU8334698A (en) | 1999-02-10 |
| EP1000358B1 (de) | 2003-04-02 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| RER | Ceased as to paragraph 5 lit. 3 law introducing patent treaties |