ATE236996T1 - Sätze von, mit fluoreszierenden energieübertragungsverbindungen, markierten primern und deren verwendung in mehrkomponentenanalyse - Google Patents

Sätze von, mit fluoreszierenden energieübertragungsverbindungen, markierten primern und deren verwendung in mehrkomponentenanalyse

Info

Publication number
ATE236996T1
ATE236996T1 AT97952413T AT97952413T ATE236996T1 AT E236996 T1 ATE236996 T1 AT E236996T1 AT 97952413 T AT97952413 T AT 97952413T AT 97952413 T AT97952413 T AT 97952413T AT E236996 T1 ATE236996 T1 AT E236996T1
Authority
AT
Austria
Prior art keywords
energy transfer
sets
component analysis
labels
fluorescent energy
Prior art date
Application number
AT97952413T
Other languages
English (en)
Inventor
Jingyue Ju
Original Assignee
Incyte Pharma Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Incyte Pharma Inc filed Critical Incyte Pharma Inc
Application granted granted Critical
Publication of ATE236996T1 publication Critical patent/ATE236996T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6818—Hybridisation assays characterised by the detection means involving interaction of two or more labels, e.g. resonant energy transfer
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
AT97952413T 1997-01-15 1997-12-12 Sätze von, mit fluoreszierenden energieübertragungsverbindungen, markierten primern und deren verwendung in mehrkomponentenanalyse ATE236996T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US08/784,162 US5804386A (en) 1997-01-15 1997-01-15 Sets of labeled energy transfer fluorescent primers and their use in multi component analysis
PCT/US1997/022914 WO1998031834A1 (en) 1997-01-15 1997-12-12 Sets of labeled energy transfer fluorescent primers and their use in multi component analysis

Publications (1)

Publication Number Publication Date
ATE236996T1 true ATE236996T1 (de) 2003-04-15

Family

ID=25131547

Family Applications (1)

Application Number Title Priority Date Filing Date
AT97952413T ATE236996T1 (de) 1997-01-15 1997-12-12 Sätze von, mit fluoreszierenden energieübertragungsverbindungen, markierten primern und deren verwendung in mehrkomponentenanalyse

Country Status (8)

Country Link
US (3) US5804386A (de)
EP (1) EP0943019B1 (de)
JP (1) JP2001509271A (de)
AT (1) ATE236996T1 (de)
AU (1) AU5602298A (de)
CA (1) CA2275139A1 (de)
DE (1) DE69720763T2 (de)
WO (1) WO1998031834A1 (de)

Families Citing this family (125)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6046005A (en) * 1997-01-15 2000-04-04 Incyte Pharmaceuticals, Inc. Nucleic acid sequencing with solid phase capturable terminators comprising a cleavable linking group
AU1366299A (en) 1997-10-27 1999-05-17 Boston Probes, Inc. Methods, kits and compositions pertaining to pna molecular beacons
US6485901B1 (en) * 1997-10-27 2002-11-26 Boston Probes, Inc. Methods, kits and compositions pertaining to linear beacons
US6537747B1 (en) 1998-02-03 2003-03-25 Lucent Technologies Inc. Data transmission using DNA oligomers
CA2319490A1 (en) * 1998-02-03 1999-08-05 Amersham Pharmacia Biotech Inc. Energy transfer dyes
WO1999049293A2 (en) 1998-03-24 1999-09-30 Boston Probes, Inc. Methods, kits and compositions pertaining to detection complexes
US6180408B1 (en) * 1998-08-21 2001-01-30 Washington University Fluorescence polarization in nucleic acid analysis
GB2341189B (en) * 1998-08-31 2001-06-13 Amersham Pharm Biotech Inc Energy transfer dyes
DE19850593A1 (de) * 1998-11-03 2000-05-04 Biochip Technologies Gmbh Verfahren zum Nachweis von Hybridisierungsereignissen bei DNA
GB9827908D0 (en) * 1998-12-19 1999-02-10 Univ Manchester Nucleic acid sequencing method
US6754375B1 (en) * 1999-07-16 2004-06-22 Packard Bioscience Company Method and system for interactively developing at least one grid pattern and computer-readable storage medium having a program for executing the method
US7209836B1 (en) 1999-07-16 2007-04-24 Perkinelmer Las, Inc. Method and system for automatically creating crosstalk-corrected data of a microarray
US6982146B1 (en) * 1999-08-30 2006-01-03 The United States Of America As Represented By The Department Of Health And Human Services High speed parallel molecular nucleic acid sequencing
US6362832B1 (en) 1999-09-01 2002-03-26 Packard Bioscience Company Method and system for overlaying at least three microarray images to obtain a multicolor composite image
US6607885B1 (en) 1999-10-15 2003-08-19 E. I. Du Pont De Nemours And Company Method for high-density microarray medicated gene expression profiling
US6528318B1 (en) * 2000-03-06 2003-03-04 The Johns Hopkins University Scatter controlled emission for optical taggants and chemical sensors
US20020072058A1 (en) * 2000-03-24 2002-06-13 Voelker Leroy L. Method for amplifying quinolone-resistance-determining-regions and identifying polymorphic variants thereof
US6323337B1 (en) 2000-05-12 2001-11-27 Molecular Probes, Inc. Quenching oligonucleotides
US6838244B1 (en) * 2000-05-19 2005-01-04 Monsanto Technology Llc Fluorescent oligonucleotides and uses thereof
EP2100971A3 (de) * 2000-07-07 2009-11-25 Visigen Biotechnologies, Inc. Echtzeit-Sequenzbestimmung
US20060057565A1 (en) * 2000-09-11 2006-03-16 Jingyue Ju Combinatorial fluorescence energy transfer tags and uses thereof
JP2004508838A (ja) * 2000-09-11 2004-03-25 ザ・トラスティーズ・オブ・コランビア・ユニバーシティー・イン・ザ・シティー・オブ・ニューヨーク 組合せ蛍光エネルギー移動タグ及びそれらの使用
US6627748B1 (en) 2000-09-11 2003-09-30 The Trustees Of Columbia University In The City Of New York Combinatorial fluorescence energy transfer tags and their applications for multiplex genetic analyses
US9708358B2 (en) 2000-10-06 2017-07-18 The Trustees Of Columbia University In The City Of New York Massive parallel method for decoding DNA and RNA
EP3034627B1 (de) 2000-10-06 2019-01-30 The Trustees of Columbia University in the City of New York Massives paralleles verfahren zum entschlüsseln von dna und rna
FR2815864B1 (fr) * 2000-10-26 2003-02-28 Oreal Utilisation de l'association d'au moins un carotenoide et de vitamine c pour traiter les signes cutanes du vieillissement
US7211414B2 (en) 2000-12-01 2007-05-01 Visigen Biotechnologies, Inc. Enzymatic nucleic acid synthesis: compositions and methods for altering monomer incorporation fidelity
US20020168641A1 (en) * 2001-03-09 2002-11-14 Bruce Mortensen Fluorescein-cyanine 5 as a fluorescence resonance energy transfer pair
DE10133308A1 (de) * 2001-07-12 2003-01-23 Praenadia Gmbh Verfahren zum Nachweis einer Nukleinsäure
DE10137530A1 (de) * 2001-08-01 2003-02-13 Presens Prec Sensing Gmbh Anordnung und Verfahren zur Mehrfach-Fluoreszenzmessung
ATE437216T1 (de) * 2001-09-06 2009-08-15 Genomic Profiling Systems Inc Schnellnachweis replizierender zellen
US6593091B2 (en) 2001-09-24 2003-07-15 Beckman Coulter, Inc. Oligonucleotide probes for detecting nucleic acids through changes in flourescence resonance energy transfer
US6852492B2 (en) 2001-09-24 2005-02-08 Intel Corporation Nucleic acid sequencing by raman monitoring of uptake of precursors during molecular replication
US6972173B2 (en) * 2002-03-14 2005-12-06 Intel Corporation Methods to increase nucleotide signals by raman scattering
US7238477B2 (en) * 2001-09-24 2007-07-03 Intel Corporation Methods to increase nucleotide signals by Raman scattering
US6982165B2 (en) * 2001-09-24 2006-01-03 Intel Corporation Nucleic acid sequencing by raman monitoring of molecular deconstruction
US20030219754A1 (en) * 2002-05-23 2003-11-27 Oleksy Jerome E. Fluorescence polarization detection of nucleic acids
US7074597B2 (en) * 2002-07-12 2006-07-11 The Trustees Of Columbia University In The City Of New York Multiplex genotyping using solid phase capturable dideoxynucleotides and mass spectrometry
WO2004055160A2 (en) * 2002-12-13 2004-07-01 The Trustees Of Columbia University In The City Of New York Biomolecular coupling methods using 1,3-dipolar cycloaddition chemistry
WO2004085670A2 (en) * 2003-03-24 2004-10-07 Perkinelmer Las, Inc. Polarization detection
WO2005077125A2 (en) * 2004-02-11 2005-08-25 Applera Corporation Methods and compositions for detecting nucleic acids
WO2005086647A2 (en) * 2004-02-23 2005-09-22 University Of Maryland, Baltimore Immuno-pcr method for the detection of a biomolecule in a test sample
US7622279B2 (en) * 2004-03-03 2009-11-24 The Trustees Of Columbia University In The City Of New York Photocleavable fluorescent nucleotides for DNA sequencing on chip constructed by site-specific coupling chemistry
WO2006073436A2 (en) * 2004-04-29 2006-07-13 The Trustees Of Columbia University In The City Of New York Mass tag pcr for multiplex diagnostics
CN101189520A (zh) * 2005-05-03 2008-05-28 亚佩瑞股份有限公司 荧光检测系统和供其使用的染料组
WO2007002204A2 (en) 2005-06-21 2007-01-04 The Trustees Of Columbia University In The City Of New York Pyrosequencing methods and related compostions
US9057046B2 (en) * 2005-09-26 2015-06-16 Rapid Micro Biosystems, Inc. Cassette containing growth medium
US8796432B2 (en) * 2005-10-31 2014-08-05 The Trustees Of Columbia University In The City Of New York Chemically cleavable 3'-o-allyl-DNTP-allyl-fluorophore fluorescent nucleotide analogues and related methods
WO2007053702A2 (en) 2005-10-31 2007-05-10 The Trustees Of Columbia University In The City Of New York Synthesis of four color 3'-o-allyl modified photocleavable fluorescent nucleotides and related methods
WO2007062105A2 (en) * 2005-11-21 2007-05-31 The Trustees Of Columbia University In The City Of New York Multiplex digital immuno-sensing using a library of photocleavable mass tags
US8703734B2 (en) 2005-12-12 2014-04-22 The United States Of America, As Represented By The Secretary, Department Of Health And Human Services Nanoprobes for detection or modification of molecules
JP2009519041A (ja) * 2005-12-12 2009-05-14 アメリカ合衆国 核酸配列決定のためのプローブおよび使用方法
CN101495656B (zh) 2006-06-07 2017-02-08 纽约哥伦比亚大学理事会 采用带修饰的核苷酸通过纳米通道进行dna序列测定
US20080032410A1 (en) * 2006-07-19 2008-02-07 Bio-Rad Laboratories, Inc. Labeling for identification of proteins and other macromolecules
US9045522B2 (en) 2006-07-31 2015-06-02 Wanli Bi Nucleic acid amplification using a reversibly modified oligonucleotide
BRPI0715427B1 (pt) * 2006-07-31 2016-04-12 Wanli Bi método para amplificação de sequência ou sinal de ácidos nucleicos alvo, oligonucleotideo modificado reversível, mistura e conjunto de reação de amplificação de ácido nucleico
US9476830B2 (en) 2006-11-21 2016-10-25 California Institute Of Technology Second harmonic imaging nanoprobes and techniques for use thereof
GB2457402B (en) 2006-12-01 2011-10-19 Univ Columbia Four-color DNA sequencing by synthesis using cleavable fluorescent nucleotide reversible terminators
US11940413B2 (en) 2007-02-05 2024-03-26 IsoPlexis Corporation Methods and devices for sequencing nucleic acids in smaller batches
EP2209911B1 (de) 2007-10-19 2013-10-16 The Trustees of Columbia University in the City of New York Dna-sequenzierung mit reversiblen nicht-fluoreszenz-nukleotid-terminatoren und mit einer spaltbaren markierung modifizierten nukleotidterminatoren und eine deoxyinosineverbindung mit einer reversiblen terminatorgruppe
EP2207900B1 (de) 2007-10-19 2015-04-29 The Trustees of Columbia University in the City of New York Konstruktion und synthese spaltbarer fluoreszenznukleotide als reversible terminatoren für die dna-sequenzierung mittels synthese
WO2009097368A2 (en) 2008-01-28 2009-08-06 Complete Genomics, Inc. Methods and compositions for efficient base calling in sequencing reactions
US9017973B2 (en) 2008-03-19 2015-04-28 Intelligent Biosystems, Inc. Methods and compositions for incorporating nucleotides
CN102016554B (zh) * 2008-04-04 2013-01-30 南达技术公司 光学检验系统及方法
CN102224410B (zh) 2008-09-24 2017-02-08 施特劳斯控股公司 用于测试分析物的成像分析仪
EP2380019A4 (de) * 2009-01-21 2017-10-25 California Institute of Technology Mehrzweckanalyse mit nanoröhrchen für harmonische zweitbildgebung
JP5730280B2 (ja) 2009-03-16 2015-06-03 パング バイオファーマ リミテッド 非カノニカル生物学的活性を有するヒスチジル−tRNAシンテターゼスプライスバリアントを含む組成物および方法
WO2010111674A2 (en) 2009-03-27 2010-09-30 Life Technologies Corporation Methods and apparatus for single molecule sequencing using energy transfer detection
WO2010120509A2 (en) 2009-03-31 2010-10-21 Atyr Pharma, Inc. Compositions and methods comprising aspartyl-trna synthetases having non-canonical biological activities
US8324914B2 (en) 2010-02-08 2012-12-04 Genia Technologies, Inc. Systems and methods for characterizing a molecule
US9605307B2 (en) * 2010-02-08 2017-03-28 Genia Technologies, Inc. Systems and methods for forming a nanopore in a lipid bilayer
US20110192723A1 (en) * 2010-02-08 2011-08-11 Genia Technologies, Inc. Systems and methods for manipulating a molecule in a nanopore
US9678055B2 (en) 2010-02-08 2017-06-13 Genia Technologies, Inc. Methods for forming a nanopore in a lipid bilayer
CA2797093C (en) 2010-04-26 2019-10-29 Atyr Pharma, Inc. Innovative discovery of therapeutic, diagnostic, and antibody compositions related to protein fragments of cysteinyl-trna synthetase
CA2797362C (en) 2010-04-27 2020-12-08 Atyr Pharma, Inc. Innovative discovery of therapeutic, diagnostic, and antibody compositions related to protein fragments of isoleucyl trna synthetases
WO2011139853A2 (en) 2010-04-28 2011-11-10 Atyr Pharma, Inc. Innovative discovery of therapeutic, diagnostic, and antibody compositions related to protein fragments of alanyl trna synthetases
EP2563936B1 (de) 2010-04-29 2018-03-14 Medical Prognosis Institute A/S Verfahren und vorrichtungen zur vorhersage der behandlungswirksamkeit
US8986680B2 (en) 2010-04-29 2015-03-24 Atyr Pharma, Inc. Innovative discovery of therapeutic, diagnostic, and antibody compositions related to protein fragments of Asparaginyl tRNA synthetases
EP2563383B1 (de) 2010-04-29 2017-03-01 Atyr Pharma, Inc. Innovative entdeckung therapeutischer, diagnostischer und antikörperhaltiger zusammensetzungen im zusammenhang mit proteinfragmenten von valyl-trna-synthetasen
CN103140233B (zh) 2010-05-03 2017-04-05 Atyr 医药公司 与甲硫氨酰‑tRNA合成酶的蛋白片段相关的治疗、诊断和抗体组合物的发现
WO2011139986A2 (en) 2010-05-03 2011-11-10 Atyr Pharma, Inc. Innovative discovery of therapeutic, diagnostic, and antibody compositions related to protein fragments of arginyl-trna synthetases
CA2797977C (en) 2010-05-03 2019-08-20 Atyr Pharma, Inc. Innovative discovery of therapeutic, diagnostic, and antibody compositions related to protein fragments of phenylalanyl-alpha-trna synthetases
EP2566499B1 (de) 2010-05-04 2017-01-25 aTyr Pharma, Inc. Innovative entdeckung therapeutischer, diagnostischer und antikörperhaltiger zusammensetzungen im zusammenhang mit proteinfragmenten aus einem p38-multi-trna-synthetase-komplex
CA2799197C (en) 2010-05-14 2019-10-29 Atyr Pharma, Inc. Innovative discovery of therapeutic, diagnostic, and antibody compositions related to protein fragments of phenylalanyl-beta-trna synthetases
US9034598B2 (en) 2010-05-17 2015-05-19 Atyr Pharma, Inc. Innovative discovery of therapeutic, diagnostic, and antibody compositions related to protein fragments of leucyl-tRNA synthetases
AU2011258106B2 (en) 2010-05-27 2017-02-23 Pangu Biopharma Limited Innovative discovery of therapeutic, diagnostic, and antibody compositions related to protein fragments of glutaminyl-tRNA synthetases
CN103118694B (zh) 2010-06-01 2016-08-03 Atyr医药公司 与赖氨酰-tRNA合成酶的蛋白片段相关的治疗、诊断和抗体组合物的发现
JP6116479B2 (ja) 2010-07-12 2017-04-19 エータイアー ファーマ, インコーポレイテッド グリシルtRNA合成酵素のタンパク質フラグメントに関連した治療用、診断用および抗体組成物の革新的発見
WO2012024642A1 (en) * 2010-08-20 2012-02-23 Life Technologies Corporation Quantitative real-time pcr assay using fret dual-labeled primers
JP5964304B2 (ja) 2010-08-25 2016-08-03 エータイアー ファーマ, インコーポレイテッド チロシルtRNA合成酵素のタンパク質フラグメントに関連した治療用、診断用および抗体組成物の革新的発見
CN103282518B (zh) 2010-12-17 2016-11-16 纽约哥伦比亚大学理事会 使用经修饰的核苷酸和纳米孔检测的dna边合成边测序
US9121059B2 (en) 2010-12-22 2015-09-01 Genia Technologies, Inc. Nanopore-based single molecule characterization
US8962242B2 (en) 2011-01-24 2015-02-24 Genia Technologies, Inc. System for detecting electrical properties of a molecular complex
US9110478B2 (en) 2011-01-27 2015-08-18 Genia Technologies, Inc. Temperature regulation of measurement arrays
WO2012162429A2 (en) 2011-05-23 2012-11-29 The Trustees Of Columbia University In The City Of New York Dna sequencing by synthesis using raman and infrared spectroscopy detection
EP2714927B1 (de) 2011-06-01 2016-08-10 Medical Prognosis Institute A/S Verfahren und vorrichtungen zur prognose eines krebsrezidivs
WO2013070730A2 (en) 2011-11-07 2013-05-16 Rapid Micro Biosystems, Inc. Cassette for sterility testing
WO2013078410A1 (en) 2011-11-21 2013-05-30 California Institute Of Technology Functionalization of and use of functionalized second harmonic generating nanoprobes
EP2814514B1 (de) 2012-02-16 2017-09-13 Atyr Pharma, Inc. Histidyl-trna-synthetasen zur behandlung von autoimmun- und entzündungserkrankungen
US8986629B2 (en) 2012-02-27 2015-03-24 Genia Technologies, Inc. Sensor circuit for controlling, detecting, and measuring a molecular complex
WO2013154999A2 (en) 2012-04-09 2013-10-17 The Trustees Of Columbia University In The City Of New York Method of preparation of nanopore and uses thereof
JP6444859B2 (ja) 2012-04-16 2018-12-26 ラピッド マイクロ バイオシステムズ インコーポレイテッド 細胞培養デバイス
JP2015525077A (ja) 2012-06-15 2015-09-03 ジェニア・テクノロジーズ・インコーポレイテッド チップの構成および高精度な核酸配列決定
ES2779699T3 (es) 2012-06-20 2020-08-18 Univ Columbia Secuenciación de ácidos nucleicos mediante detección en nanoporos de moléculas de etiqueta
US9605309B2 (en) 2012-11-09 2017-03-28 Genia Technologies, Inc. Nucleic acid sequencing using tags
CN104937111B (zh) 2012-11-27 2018-05-11 智利天主教教皇大学 用于诊断甲状腺肿瘤的组合物和方法
US9759711B2 (en) 2013-02-05 2017-09-12 Genia Technologies, Inc. Nanopore arrays
US10732183B2 (en) 2013-03-15 2020-08-04 The Trustees Of Columbia University In The City Of New York Method for detecting multiple predetermined compounds in a sample
WO2014144883A1 (en) 2013-03-15 2014-09-18 The Trustees Of Columbia University In The City Of New York Raman cluster tagged molecules for biological imaging
WO2014195032A1 (en) 2013-06-07 2014-12-11 Medical Prognosis Institute A/S Methods and devices for predicting treatment efficacy of fulvestrant in cancer patients
US9551697B2 (en) 2013-10-17 2017-01-24 Genia Technologies, Inc. Non-faradaic, capacitively coupled measurement in a nanopore cell array
US9567630B2 (en) 2013-10-23 2017-02-14 Genia Technologies, Inc. Methods for forming lipid bilayers on biochips
EP3060918B1 (de) 2013-10-23 2019-09-18 Genia Technologies, Inc. Schnelle molekulare erfassung mit nanoporen
US10240195B2 (en) 2014-03-24 2019-03-26 The Trustees Of Columbia University In The City Of New York Chemical methods for producing tagged nucleotides
US11214836B2 (en) 2014-07-15 2022-01-04 Ontario Institute For Cancer Research Methods and devices for predicting anthracycline treatment efficacy
CN108779138B (zh) 2015-09-28 2022-06-17 哥伦比亚大学董事会 用作dna合成测序的可逆终止物的基于新的二硫键接头的核苷酸的设计与合成
CN116656795A (zh) 2016-05-23 2023-08-29 纽约哥伦比亚大学董事会 核苷酸衍生物及其使用方法
WO2018165207A1 (en) 2017-03-06 2018-09-13 Singular Genomic Systems, Inc. Nucleic acid sequencing-by-synthesis (sbs) methods that combine sbs cycle steps
CN119639879A (zh) 2017-03-28 2025-03-18 哥伦比亚大学董事会 用于连接荧光标记与用于dna合成测序的碱基的具有不同可裂解连接子的3'-o-改性的核苷酸类似物
WO2019164977A1 (en) 2018-02-21 2019-08-29 Singular Genomics Systems, Inc. Fluorinated nucleotides and uses thereof
US12188086B2 (en) 2018-03-15 2025-01-07 The Trustees Of Columbia University In The City Of New York Nucleotide analogues and use thereof for nucleic acid sequencing and analysis preliminary class
US12031985B2 (en) 2018-04-19 2024-07-09 First Light Diagnostics, Inc. Detection of targets
CA3115279A1 (en) 2018-10-04 2020-04-09 First Light Diagnostics, Inc. Analysis instrument
US12145960B2 (en) 2018-10-25 2024-11-19 Singular Genomics Systems, Inc. Nucleotide analogues
US11970735B2 (en) 2019-01-08 2024-04-30 Singular Genomics Systems, Inc. Nucleotide cleavable linkers and uses thereof

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4996143A (en) * 1985-12-23 1991-02-26 Syngene, Inc. Fluorescent stokes shift probes for polynucleotide hybridization
US5326692B1 (en) * 1992-05-13 1996-04-30 Molecular Probes Inc Fluorescent microparticles with controllable enhanced stokes shift
IE66205B1 (en) * 1990-06-14 1995-12-13 Paul A Bartlett Polypeptide analogs
JP3097205B2 (ja) * 1991-09-05 2000-10-10 株式会社日立製作所 電気泳動分離検出方法
US5654419A (en) * 1994-02-01 1997-08-05 The Regents Of The University Of California Fluorescent labels and their use in separations
US5800996A (en) * 1996-05-03 1998-09-01 The Perkin Elmer Corporation Energy transfer dyes with enchanced fluorescence

Also Published As

Publication number Publication date
US5814454A (en) 1998-09-29
CA2275139A1 (en) 1998-07-23
JP2001509271A (ja) 2001-07-10
AU5602298A (en) 1998-08-07
US5804386A (en) 1998-09-08
US5952180A (en) 1999-09-14
EP0943019B1 (de) 2003-04-09
DE69720763D1 (de) 2003-05-15
EP0943019A1 (de) 1999-09-22
WO1998031834A1 (en) 1998-07-23
DE69720763T2 (de) 2004-03-11

Similar Documents

Publication Publication Date Title
DE69720763D1 (de) Sätze von, mit fluoreszierenden energieübertragungsverbindungen, markierten primern und deren verwendung in mehrkomponentenanalyse
DE69530286D1 (de) Mit energieübertragungsvermittelnden verbindungen markierte proben
EP3035037B1 (de) Vorrichtung zur differenzierung mehrerer fluoreszenzsignale nach anregungswellenlänge
DE69635089D1 (de) Fluoreszierende Markierungskomplexe mit grossem Stokes-Shift die durch Verbindung von Cyaninen und anderen resonanzenergieübertragungsfähigen Fluorochromen zusammengestellt sind
DK0713921T3 (da) Fremgangsmåde til dæmpning af fluorescensen i opløsning af fluorescensmærkede oligonukleotidprober
EP0670041A4 (de) Fluoreszenzenergieübertragung und intramolekulare energieübertragung in teilchen unter verwendung neuer verbindungen.
DE60042738D1 (de) Verfahren zum nachweis von analyten mit hilfe von halbleiternanokrystallen
PT833939E (pt) Reagentes de camuflagem e ensaios para luminescencia mediada por enzima
DE60005787D1 (de) Wasserlösliche rhodaminfarbstoffe und ihre konjugate
AU3481297A (en) Monitoring hybridization during pcr
DE69838090D1 (de) Mehrgefässplatten mit kleiner störstrahlung für fluoreszenzmessungen von biologischen und biochemischen proben
DK1086179T3 (da) Energioverførselsassaymetode og reagens
DE60108799D1 (de) Fluoreszente Nucleotide, enthaltend einen Cyanin, Merocyanin- oder Styryl-Farbstoff, zur Erkennung von Nucleinsäuren
ATE133257T1 (de) Ermittlung und quantifizierung mehrerer analyten unter verwendung von markierungstechniken
ATE306669T1 (de) Nitroreduktase reporter-gen assay mit fluoreszenz erhöhung
CA2342767A1 (en) Multihued labels
DE60334639D1 (de) Kassetten mit heterozyklischenfret-farbstoffen zur markierung biologischer moleküle und ihre verwendung beider sequenzierung von dna
EP1489418A4 (de) Neue fluoreszenzmarkierungsverbindungen
Rusling et al. Triplex-directed covalent cross-linking of a DNA nanostructure
DE69900465D1 (de) Ein screeningverfahren, basierend auf fluoreszenzpolarisation
BR0112400A (pt) Ensaio de hibridização triplex mediado por cátions
US20030035755A1 (en) Organic electroluminescence (OEL)-based biochips
EP1321520A4 (de) Neuartige nukleinsäuresonde und neuartiges verfahren zur analyse von nukleinsäuren unter verwendung derselben
SE9901306D0 (sv) Improved TIRF chamber
Laitala et al. Time-resolved detection probe for homogeneous nucleic acid analyses in one-step format

Legal Events

Date Code Title Description
REZ Deleted due to withdrawal