ATE236996T1 - Sätze von, mit fluoreszierenden energieübertragungsverbindungen, markierten primern und deren verwendung in mehrkomponentenanalyse - Google Patents

Sätze von, mit fluoreszierenden energieübertragungsverbindungen, markierten primern und deren verwendung in mehrkomponentenanalyse

Info

Publication number
ATE236996T1
ATE236996T1 AT97952413T AT97952413T ATE236996T1 AT E236996 T1 ATE236996 T1 AT E236996T1 AT 97952413 T AT97952413 T AT 97952413T AT 97952413 T AT97952413 T AT 97952413T AT E236996 T1 ATE236996 T1 AT E236996T1
Authority
AT
Austria
Prior art keywords
energy transfer
sets
component analysis
labels
fluorescent energy
Prior art date
Application number
AT97952413T
Other languages
English (en)
Inventor
Jingyue Ju
Original Assignee
Incyte Pharma Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Incyte Pharma Inc filed Critical Incyte Pharma Inc
Application granted granted Critical
Publication of ATE236996T1 publication Critical patent/ATE236996T1/de

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6818—Hybridisation assays characterised by the detection means involving interaction of two or more labels, e.g. resonant energy transfer
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
AT97952413T 1997-01-15 1997-12-12 Sätze von, mit fluoreszierenden energieübertragungsverbindungen, markierten primern und deren verwendung in mehrkomponentenanalyse ATE236996T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US08/784,162 US5804386A (en) 1997-01-15 1997-01-15 Sets of labeled energy transfer fluorescent primers and their use in multi component analysis
PCT/US1997/022914 WO1998031834A1 (en) 1997-01-15 1997-12-12 Sets of labeled energy transfer fluorescent primers and their use in multi component analysis

Publications (1)

Publication Number Publication Date
ATE236996T1 true ATE236996T1 (de) 2003-04-15

Family

ID=25131547

Family Applications (1)

Application Number Title Priority Date Filing Date
AT97952413T ATE236996T1 (de) 1997-01-15 1997-12-12 Sätze von, mit fluoreszierenden energieübertragungsverbindungen, markierten primern und deren verwendung in mehrkomponentenanalyse

Country Status (8)

Country Link
US (3) US5804386A (de)
EP (1) EP0943019B1 (de)
JP (1) JP2001509271A (de)
AT (1) ATE236996T1 (de)
AU (1) AU5602298A (de)
CA (1) CA2275139A1 (de)
DE (1) DE69720763T2 (de)
WO (1) WO1998031834A1 (de)

Families Citing this family (125)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6046005A (en) * 1997-01-15 2000-04-04 Incyte Pharmaceuticals, Inc. Nucleic acid sequencing with solid phase capturable terminators comprising a cleavable linking group
US6355421B1 (en) * 1997-10-27 2002-03-12 Boston Probes, Inc. Methods, kits and compositions pertaining to PNA molecular beacons
US6485901B1 (en) 1997-10-27 2002-11-26 Boston Probes, Inc. Methods, kits and compositions pertaining to linear beacons
US6537747B1 (en) 1998-02-03 2003-03-25 Lucent Technologies Inc. Data transmission using DNA oligomers
JP2002502034A (ja) * 1998-02-03 2002-01-22 アマーシャム・ファルマシア・バイオテック・インコーポレーテッド エネルギー転移色素
WO1999049293A2 (en) 1998-03-24 1999-09-30 Boston Probes, Inc. Methods, kits and compositions pertaining to detection complexes
US6180408B1 (en) * 1998-08-21 2001-01-30 Washington University Fluorescence polarization in nucleic acid analysis
GB2341189B (en) * 1998-08-31 2001-06-13 Amersham Pharm Biotech Inc Energy transfer dyes
DE19850593A1 (de) * 1998-11-03 2000-05-04 Biochip Technologies Gmbh Verfahren zum Nachweis von Hybridisierungsereignissen bei DNA
GB9827908D0 (en) * 1998-12-19 1999-02-10 Univ Manchester Nucleic acid sequencing method
US6754375B1 (en) * 1999-07-16 2004-06-22 Packard Bioscience Company Method and system for interactively developing at least one grid pattern and computer-readable storage medium having a program for executing the method
US7209836B1 (en) 1999-07-16 2007-04-24 Perkinelmer Las, Inc. Method and system for automatically creating crosstalk-corrected data of a microarray
US6982146B1 (en) * 1999-08-30 2006-01-03 The United States Of America As Represented By The Department Of Health And Human Services High speed parallel molecular nucleic acid sequencing
US6362832B1 (en) 1999-09-01 2002-03-26 Packard Bioscience Company Method and system for overlaying at least three microarray images to obtain a multicolor composite image
US6607885B1 (en) 1999-10-15 2003-08-19 E. I. Du Pont De Nemours And Company Method for high-density microarray medicated gene expression profiling
US6528318B1 (en) * 2000-03-06 2003-03-04 The Johns Hopkins University Scatter controlled emission for optical taggants and chemical sensors
GB2364054B (en) * 2000-03-24 2002-05-29 Smithkline Beecham Corp Method of amplifying quinolone-resistance-determining-regions and identifying polymorphic variants thereof
US6323337B1 (en) 2000-05-12 2001-11-27 Molecular Probes, Inc. Quenching oligonucleotides
US6838244B1 (en) * 2000-05-19 2005-01-04 Monsanto Technology Llc Fluorescent oligonucleotides and uses thereof
ATE377093T1 (de) * 2000-07-07 2007-11-15 Visigen Biotechnologies Inc Sequenzbestimmung in echtzeit
CA2421582A1 (en) * 2000-09-11 2002-03-21 The Trustees Of Columbia University In The City Of New York Combinatorial fluorescence energy transfer tags and uses thereof
US6627748B1 (en) 2000-09-11 2003-09-30 The Trustees Of Columbia University In The City Of New York Combinatorial fluorescence energy transfer tags and their applications for multiplex genetic analyses
US20060057565A1 (en) * 2000-09-11 2006-03-16 Jingyue Ju Combinatorial fluorescence energy transfer tags and uses thereof
ATE356222T1 (de) 2000-10-06 2007-03-15 Univ Columbia Massives parallelverfahren zur dekodierung von dna und rna
US9708358B2 (en) 2000-10-06 2017-07-18 The Trustees Of Columbia University In The City Of New York Massive parallel method for decoding DNA and RNA
FR2815864B1 (fr) * 2000-10-26 2003-02-28 Oreal Utilisation de l'association d'au moins un carotenoide et de vitamine c pour traiter les signes cutanes du vieillissement
US7211414B2 (en) 2000-12-01 2007-05-01 Visigen Biotechnologies, Inc. Enzymatic nucleic acid synthesis: compositions and methods for altering monomer incorporation fidelity
US20020168641A1 (en) * 2001-03-09 2002-11-14 Bruce Mortensen Fluorescein-cyanine 5 as a fluorescence resonance energy transfer pair
DE10133308A1 (de) * 2001-07-12 2003-01-23 Praenadia Gmbh Verfahren zum Nachweis einer Nukleinsäure
DE10137530A1 (de) * 2001-08-01 2003-02-13 Presens Prec Sensing Gmbh Anordnung und Verfahren zur Mehrfach-Fluoreszenzmessung
CA2459320C (en) 2001-09-06 2016-07-05 Genomic Profiling Systems, Inc. Rapid detection of replicating cells
US6852492B2 (en) * 2001-09-24 2005-02-08 Intel Corporation Nucleic acid sequencing by raman monitoring of uptake of precursors during molecular replication
US7238477B2 (en) * 2001-09-24 2007-07-03 Intel Corporation Methods to increase nucleotide signals by Raman scattering
US6982165B2 (en) 2001-09-24 2006-01-03 Intel Corporation Nucleic acid sequencing by raman monitoring of molecular deconstruction
US6593091B2 (en) 2001-09-24 2003-07-15 Beckman Coulter, Inc. Oligonucleotide probes for detecting nucleic acids through changes in flourescence resonance energy transfer
US6972173B2 (en) * 2002-03-14 2005-12-06 Intel Corporation Methods to increase nucleotide signals by raman scattering
US20030219754A1 (en) * 2002-05-23 2003-11-27 Oleksy Jerome E. Fluorescence polarization detection of nucleic acids
US7074597B2 (en) * 2002-07-12 2006-07-11 The Trustees Of Columbia University In The City Of New York Multiplex genotyping using solid phase capturable dideoxynucleotides and mass spectrometry
WO2004055160A2 (en) * 2002-12-13 2004-07-01 The Trustees Of Columbia University In The City Of New York Biomolecular coupling methods using 1,3-dipolar cycloaddition chemistry
US20060192960A1 (en) * 2003-03-24 2006-08-31 Rencs Erik V Polarization detection
WO2005077125A2 (en) * 2004-02-11 2005-08-25 Applera Corporation Methods and compositions for detecting nucleic acids
US20050239108A1 (en) * 2004-02-23 2005-10-27 University Of Maryland, Baltimore Immuno-PCR method for the detection of a biomolecule in a test sample
CA2557818A1 (en) * 2004-03-03 2005-09-15 The Trustees Of Columbia University In The City Of New York Photocleavable fluorescent nucleotides for dna sequencing on chip constructed by site-specific coupling chemistry
WO2006073436A2 (en) * 2004-04-29 2006-07-13 The Trustees Of Columbia University In The City Of New York Mass tag pcr for multiplex diagnostics
EP1886145A2 (de) * 2005-05-03 2008-02-13 Applera Corporation Fluoreszenznachweissystem und farbstoffsatz zur verwendung damit
WO2007002204A2 (en) 2005-06-21 2007-01-04 The Trustees Of Columbia University In The City Of New York Pyrosequencing methods and related compostions
CA2623408C (en) * 2005-09-26 2014-04-01 Rapid Micro Biosystems, Inc. Cassette containing growth medium
WO2007053719A2 (en) * 2005-10-31 2007-05-10 The Trustees Of Columbia University In The City Of New York Chemically cleavable 3'-o-allyl-dntp-allyl-fluorophore fluorescent nucleotide analogues and related methods
US7982029B2 (en) 2005-10-31 2011-07-19 The Trustees Of Columbia University In The City Of New York Synthesis of four color 3′O-allyl, modified photocleavable fluorescent nucleotides and related methods
CA2630544A1 (en) * 2005-11-21 2007-05-31 The Trustees Of Columbia University In The City Of New York Multiplex digital immuno-sensing using a library of photocleavable mass tags
US8703734B2 (en) 2005-12-12 2014-04-22 The United States Of America, As Represented By The Secretary, Department Of Health And Human Services Nanoprobes for detection or modification of molecules
ATE481505T1 (de) * 2005-12-12 2010-10-15 Us Gov Health & Human Serv Sonde zum sequenzieren von nukleinsäure und verwendungsverfahren
US8889348B2 (en) 2006-06-07 2014-11-18 The Trustees Of Columbia University In The City Of New York DNA sequencing by nanopore using modified nucleotides
US20080032410A1 (en) * 2006-07-19 2008-02-07 Bio-Rad Laboratories, Inc. Labeling for identification of proteins and other macromolecules
CN101495657B (zh) * 2006-07-31 2016-08-10 毕万里 使用可逆修饰寡核苷酸扩增核酸
US9045522B2 (en) 2006-07-31 2015-06-02 Wanli Bi Nucleic acid amplification using a reversibly modified oligonucleotide
US9476830B2 (en) 2006-11-21 2016-10-25 California Institute Of Technology Second harmonic imaging nanoprobes and techniques for use thereof
DE112007002932B4 (de) 2006-12-01 2015-08-06 The Trustees Of Columbia University In The City Of New York Vierfarben DNA-Sequenzierung mittels Synthese unter Verwendung von abspaltbaren, reversiblen, fluoreszierenden Nucleotidterminatoren
US11940413B2 (en) 2007-02-05 2024-03-26 IsoPlexis Corporation Methods and devices for sequencing nucleic acids in smaller batches
US9115163B2 (en) 2007-10-19 2015-08-25 The Trustees Of Columbia University In The City Of New York DNA sequence with non-fluorescent nucleotide reversible terminators and cleavable label modified nucleotide terminators
EP4310194A3 (de) 2007-10-19 2024-10-16 The Trustees of Columbia University in the City of New York Entwurf und synthese von spaltbaren fluoreszierenden nukleotiden als reversible terminatoren zur dna-sequenzierung durch synthese
US8617811B2 (en) 2008-01-28 2013-12-31 Complete Genomics, Inc. Methods and compositions for efficient base calling in sequencing reactions
US9017973B2 (en) 2008-03-19 2015-04-28 Intelligent Biosystems, Inc. Methods and compositions for incorporating nucleotides
DE112009000832T5 (de) * 2008-04-04 2011-07-28 Nanda Technologies GmbH, 85716 System und Verfahren zur optischen Inspektion
AU2009296526B2 (en) 2008-09-24 2015-11-19 First Light Diagnostics, Inc. Method for detecting analytes
EP2380019A4 (de) * 2009-01-21 2017-10-25 California Institute of Technology Mehrzweckanalyse mit nanoröhrchen für harmonische zweitbildgebung
EP2408905B1 (de) 2009-03-16 2017-05-10 Pangu Biopharma Limited Zusammensetzungen und verfahren mit histidyl-trna-synthetase-spleissvarianten mit nicht-kanonischen biologischen aktivitäten
WO2010111691A2 (en) 2009-03-27 2010-09-30 Life Technologies Corp Conjugates of biomolecules to nanoparticles
AU2010236913B2 (en) 2009-03-31 2016-03-24 Atyr Pharma, Inc. Compositions and methods comprising aspartyl-tRNA synthetases having non-canonical biological activities
US9678055B2 (en) 2010-02-08 2017-06-13 Genia Technologies, Inc. Methods for forming a nanopore in a lipid bilayer
US9605307B2 (en) 2010-02-08 2017-03-28 Genia Technologies, Inc. Systems and methods for forming a nanopore in a lipid bilayer
US8324914B2 (en) 2010-02-08 2012-12-04 Genia Technologies, Inc. Systems and methods for characterizing a molecule
US20110192723A1 (en) * 2010-02-08 2011-08-11 Genia Technologies, Inc. Systems and methods for manipulating a molecule in a nanopore
AU2011248625B2 (en) 2010-04-26 2017-01-05 Pangu Biopharma Limited Innovative discovery of therapeutic, diagnostic, and antibody compositions related to protein fragments of cysteinyl-tRNA synthetase
JP6294074B2 (ja) 2010-04-27 2018-03-14 エータイアー ファーマ， インコーポレイテッド イソロイシルｔＲＮＡ合成酵素のタンパク質フラグメントに関連した治療用、診断用および抗体組成物の革新的発見
CN103097524B (zh) 2010-04-28 2016-08-03 Atyr医药公司 与丙氨酰-tRNA合成酶的蛋白片段相关的治疗、诊断和抗体组合物的创新发现
WO2011150279A2 (en) 2010-05-27 2011-12-01 Atyr Pharma, Inc. Innovative discovery of therapeutic, diagnostic, and antibody compositions related to protein fragments of glutaminyl-trna synthetases
CA2797374C (en) 2010-04-29 2021-02-16 Pangu Biopharma Limited Innovative discovery of therapeutic, diagnostic, and antibody compositions related to protein fragments of asparaginyl trna synthetases
WO2011135459A2 (en) 2010-04-29 2011-11-03 Medical Prognosis Institute A/S Methods and devices for predicting treatment efficacy
AU2011248457B2 (en) 2010-04-29 2017-02-16 Pangu Biopharma Limited Innovative discovery of therapeutic, diagnostic, and antibody compositions related to protein fragments of valyl tRNA synthetases
WO2011139986A2 (en) 2010-05-03 2011-11-10 Atyr Pharma, Inc. Innovative discovery of therapeutic, diagnostic, and antibody compositions related to protein fragments of arginyl-trna synthetases
WO2011140135A2 (en) 2010-05-03 2011-11-10 Atyr Pharma, Inc. Innovative discovery of therapeutic, diagnostic, and antibody compositions related to protein fragments of methionyl-trna synthetases
CA2797977C (en) 2010-05-03 2019-08-20 Atyr Pharma, Inc. Innovative discovery of therapeutic, diagnostic, and antibody compositions related to protein fragments of phenylalanyl-alpha-trna synthetases
CA2798139C (en) 2010-05-04 2019-09-24 Atyr Pharma, Inc. Innovative discovery of therapeutic, diagnostic, and antibody compositions related to protein fragments of p38 multi-trna synthetase complex
CN103200953B (zh) 2010-05-14 2017-02-15 Atyr 医药公司 与苯丙氨酰‑β‑tRNA合成酶的蛋白片段相关的治疗、诊断和抗体组合物的创新发现
CN103096914B (zh) 2010-05-17 2015-08-12 Atyr医药公司 与亮氨酰-tRNA合成酶的蛋白片段相关的治疗、诊断和抗体组合物的创新发现
CA2800281C (en) 2010-06-01 2021-01-12 Atyr Pharma, Inc. Innovative discovery of therapeutic, diagnostic, and antibody compositions related to protein fragments of lysyl-trna synthetases
AU2011289831C1 (en) 2010-07-12 2017-06-15 Pangu Biopharma Limited Innovative discovery of therapeutic, diagnostic, and antibody compositions related to protein fragments of glycyl-tRNA synthetases
US20120058481A1 (en) * 2010-08-20 2012-03-08 Life Technologies Corporation Quantitative Real Time PCR Assay Using FRET Dual-Labeled Primers
CN103108650A (zh) 2010-08-25 2013-05-15 Atyr医药公司 与酪氨酰-tRNA合成酶的蛋白片段相关的治疗、诊断和抗体组合物的创新发现
EP2652153B1 (de) 2010-12-17 2017-07-05 The Trustees of Columbia University in the City of New York Dna-sequenzierung durch synthese unter verwendung modifizierter nukleotide und nanoporennachweis
WO2012088339A2 (en) 2010-12-22 2012-06-28 Genia Technologies, Inc. Nanopore-based single dna molecule characterization using speed bumps
US8962242B2 (en) 2011-01-24 2015-02-24 Genia Technologies, Inc. System for detecting electrical properties of a molecular complex
US9110478B2 (en) 2011-01-27 2015-08-18 Genia Technologies, Inc. Temperature regulation of measurement arrays
US9624539B2 (en) 2011-05-23 2017-04-18 The Trustees Of Columbia University In The City Of New York DNA sequencing by synthesis using Raman and infrared spectroscopy detection
US20140106986A1 (en) 2011-06-01 2014-04-17 Medical Prognosis Institute A/S Methods and devices for prognosis of cancer relapse
DK3327110T3 (en) 2011-11-07 2020-04-27 Rapid Micro Biosystems Inc Kassette til sterilitetstest
WO2013078410A1 (en) 2011-11-21 2013-05-30 California Institute Of Technology Functionalization of and use of functionalized second harmonic generating nanoprobes
IN2014MN01642A (de) 2012-02-16 2015-07-03 Atyr Pharma Inc
US8986629B2 (en) 2012-02-27 2015-03-24 Genia Technologies, Inc. Sensor circuit for controlling, detecting, and measuring a molecular complex
US10246479B2 (en) 2012-04-09 2019-04-02 The Trustees Of Columbia University In The City Of New York Method of preparation of nanopore and uses thereof
EP2839021B1 (de) 2012-04-16 2022-02-23 Rapid Micro Biosystems, Inc. Zellkultivierungsvorrichtung
GB2510719A (en) 2012-06-15 2014-08-13 Genia Technologies Inc Chip set-up and high-accuracy nucleic acid sequencing
EP2864502B1 (de) 2012-06-20 2019-10-23 The Trustees of Columbia University in the City of New York Nucleinsäuresequenzierung durch nanoporendetektion von markierungsmolekülen
US9605309B2 (en) 2012-11-09 2017-03-28 Genia Technologies, Inc. Nucleic acid sequencing using tags
MX367366B (es) 2012-11-27 2019-08-16 Univ Pontificia Catolica Chile Composiciones y metodos para diagnosticar tumores de tiroides.
US9759711B2 (en) 2013-02-05 2017-09-12 Genia Technologies, Inc. Nanopore arrays
CN105102627B (zh) 2013-03-15 2018-10-19 纽约哥伦比亚大学理事会 用于检测样品中多种预定化合物的方法
US10648026B2 (en) 2013-03-15 2020-05-12 The Trustees Of Columbia University In The City Of New York Raman cluster tagged molecules for biological imaging
WO2014195032A1 (en) 2013-06-07 2014-12-11 Medical Prognosis Institute A/S Methods and devices for predicting treatment efficacy of fulvestrant in cancer patients
US9551697B2 (en) 2013-10-17 2017-01-24 Genia Technologies, Inc. Non-faradaic, capacitively coupled measurement in a nanopore cell array
EP3640349A3 (de) 2013-10-23 2020-07-29 Roche Sequencing Solutions, Inc. Schnelle molekulare erfassung mit nanoporen
US9322062B2 (en) 2013-10-23 2016-04-26 Genia Technologies, Inc. Process for biosensor well formation
CA2943952A1 (en) 2014-03-24 2015-10-01 The Trustees Of Columbia University In The City Of New York Chemical methods for producing tagged nucleotides
WO2016008048A1 (en) 2014-07-15 2016-01-21 Ontario Institute For Cancer Research Methods and devices for predicting anthracycline treatment efficacy
US11085076B2 (en) 2015-09-28 2021-08-10 The Trustees Of Columbia University In The City Of New York Synthesis of novel disulfide linker based nucleotides as reversible terminators for DNA sequencing by synthesis
EP3463381A4 (de) 2016-05-23 2020-01-01 The Trustees of Columbia University in the City of New York Nukleotidderivate und verfahren zur verwendung davon
US11591647B2 (en) 2017-03-06 2023-02-28 Singular Genomics Systems, Inc. Nucleic acid sequencing-by-synthesis (SBS) methods that combine SBS cycle steps
CN110709089B (zh) 2017-03-28 2024-12-06 哥伦比亚大学董事会 用于连接荧光标记与用于dna合成测序的碱基的具有不同可裂解连接子的3’-o-改性的核苷酸类似物
US12215124B2 (en) 2018-02-21 2025-02-04 Singular Genomics Systems, Inc. Fluorinated nucleotides and uses thereof
WO2019178393A1 (en) 2018-03-15 2019-09-19 The Trustees Of Columbia University In The City Of New York Nucleotide analogues and use thereof for nucleic acid sequencing and analysis
CN119804399A (zh) 2018-04-19 2025-04-11 曙光诊断学公司 靶标检测
AU2019353145B2 (en) 2018-10-04 2025-12-04 First Light Diagnostics, Inc. Microbial analysis without cell purification
WO2020086834A1 (en) 2018-10-25 2020-04-30 Singular Genomics Systems, Inc. Nucleotide analogues
CN113453692A (zh) 2019-01-08 2021-09-28 奇异基因组学系统公司 核苷酸可裂解接头和其用途

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4996143A (en) * 1985-12-23 1991-02-26 Syngene, Inc. Fluorescent stokes shift probes for polynucleotide hybridization
US5326692B1 (en) * 1992-05-13 1996-04-30 Molecular Probes Inc Fluorescent microparticles with controllable enhanced stokes shift
IE66205B1 (en) * 1990-06-14 1995-12-13 Paul A Bartlett Polypeptide analogs
JP3097205B2 (ja) * 1991-09-05 2000-10-10 株式会社日立製作所 電気泳動分離検出方法
US5654419A (en) * 1994-02-01 1997-08-05 The Regents Of The University Of California Fluorescent labels and their use in separations
US5800996A (en) * 1996-05-03 1998-09-01 The Perkin Elmer Corporation Energy transfer dyes with enchanced fluorescence

Also Published As

Publication number Publication date
CA2275139A1 (en) 1998-07-23
DE69720763D1 (de) 2003-05-15
DE69720763T2 (de) 2004-03-11
US5952180A (en) 1999-09-14
EP0943019B1 (de) 2003-04-09
US5814454A (en) 1998-09-29
WO1998031834A1 (en) 1998-07-23
AU5602298A (en) 1998-08-07
EP0943019A1 (de) 1999-09-22
US5804386A (en) 1998-09-08
JP2001509271A (ja) 2001-07-10

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