ATE238341T1 - Verfahren zur reinigung von rekombinanaten proteinen - Google Patents

Verfahren zur reinigung von rekombinanaten proteinen

Info

Publication number
ATE238341T1
ATE238341T1 AT97939436T AT97939436T ATE238341T1 AT E238341 T1 ATE238341 T1 AT E238341T1 AT 97939436 T AT97939436 T AT 97939436T AT 97939436 T AT97939436 T AT 97939436T AT E238341 T1 ATE238341 T1 AT E238341T1
Authority
AT
Austria
Prior art keywords
proteins
recombinate
purifying
sup
metal ion
Prior art date
Application number
AT97939436T
Other languages
English (en)
Inventor
Paul S Nelson
Te-Tuan Yang
Steven R Kain
Thomas H Smith
Brian Perry
Original Assignee
Clontech Lab Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Clontech Lab Inc filed Critical Clontech Lab Inc
Application granted granted Critical
Publication of ATE238341T1 publication Critical patent/ATE238341T1/de

Links

Classifications

    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01DSEPARATION
    • B01D15/00Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08Selective adsorption, e.g. chromatography
    • B01D15/26Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/38Selective adsorption, e.g. chromatography characterised by the separation mechanism involving specific interaction not covered by one or more of groups B01D15/265 and B01D15/30 - B01D15/36, e.g. affinity, ligand exchange or chiral chromatography
    • B01D15/3804Affinity chromatography
    • B01D15/3828Ligand exchange chromatography, e.g. complexation, chelation or metal interaction chromatography
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01JCHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/30Processes for preparing, regenerating, or reactivating
    • B01J20/32Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating
    • B01J20/3231Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating characterised by the coating or impregnating layer
    • B01J20/3242Layers with a functional group, e.g. an affinity material, a ligand, a reactant or a complexing group
    • B01J20/3244Non-macromolecular compounds
    • B01J20/3265Non-macromolecular compounds with an organic functional group containing a metal, e.g. a metal affinity ligand
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01JCHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J45/00Ion-exchange in which a complex or a chelate is formed; Use of material as complex or chelate forming ion-exchangers; Treatment of material for improving the complex or chelate forming ion-exchange properties
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K1/00General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14Extraction; Separation; Purification
    • C07K1/16Extraction; Separation; Purification by chromatography
    • C07K1/22Affinity chromatography or related techniques based upon selective absorption processes
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K14/00Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/43504Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates
    • C07K14/43595Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates from coelenteratae, e.g. medusae
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004Oxidoreductases (1.)
    • C12N9/0012Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7)
    • C12N9/0026Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on CH-NH groups of donors (1.5)
    • C12N9/0028Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on CH-NH groups of donors (1.5) with NAD or NADP as acceptor (1.5.1)
    • C12N9/003Dihydrofolate reductase [DHFR] (1.5.1.3)
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2319/00Fusion polypeptide
    • C07K2319/20Fusion polypeptide containing a tag with affinity for a non-protein ligand
    • C07K2319/21Fusion polypeptide containing a tag with affinity for a non-protein ligand containing a His-tag
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02Column chromatography

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Toxicology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • General Engineering & Computer Science (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Solid-Sorbent Or Filter-Aiding Compositions (AREA)
  • Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
AT97939436T 1996-08-16 1997-08-18 Verfahren zur reinigung von rekombinanaten proteinen ATE238341T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US08/698,747 US5962641A (en) 1996-08-16 1996-08-16 Method for purification of recombinant proteins
PCT/US1997/014487 WO1998006739A1 (en) 1996-08-16 1997-08-18 Method for purification of recombinant proteins

Publications (1)

Publication Number Publication Date
ATE238341T1 true ATE238341T1 (de) 2003-05-15

Family

ID=24806511

Family Applications (1)

Application Number Title Priority Date Filing Date
AT97939436T ATE238341T1 (de) 1996-08-16 1997-08-18 Verfahren zur reinigung von rekombinanaten proteinen

Country Status (8)

Country Link
US (4) US5962641A (de)
EP (1) EP0935606B1 (de)
JP (1) JP3946772B2 (de)
AT (1) ATE238341T1 (de)
DE (1) DE69721289T2 (de)
DK (1) DK0935606T3 (de)
ES (1) ES2195168T3 (de)
WO (1) WO1998006739A1 (de)

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WO2000024910A1 (en) * 1998-10-28 2000-05-04 Smithkline Beecham Plc Process of purification of low density lipoprotein associated phospholipase a2 using immobilized metal affinity chromatography
US6232083B1 (en) * 1999-03-12 2001-05-15 The Research Foundation Of State University Of New York Methods and compositions for screening cloned proteins
GB2367818A (en) * 2000-10-11 2002-04-17 Kalibrant Ltd A linker and a method of chemical synthesis
DE10106339A1 (de) * 2001-02-09 2002-08-22 Hans-Joachim Mueller Verfahren zum Hochdurchsatz-Screening von Kandidaten-Verbindungen über die Hochdurchsatz-Direktsynthese von rekombinanten Polypeptiden
US20040180415A1 (en) * 2001-05-15 2004-09-16 Tchaga Grigoriy S. Methods and compositions for protein purification
US6703498B2 (en) 2001-06-21 2004-03-09 Clontech Laboratories, Inc. Water-soluble polymeric metal ion affinity compositions and methods for using the same
US7151167B2 (en) * 2002-06-10 2006-12-19 Phynexus, Inc. Open channel solid phase extraction systems and methods
US7122640B2 (en) * 2002-06-10 2006-10-17 Phynexus, Inc. Open channel solid phase extraction systems and methods
US7879621B2 (en) * 2003-05-08 2011-02-01 Phynexus, Inc. Open channel solid phase extraction systems and methods
EP1518115A2 (de) * 2002-06-10 2005-03-30 Phynexus, Inc. Offenkanalfestphasenbiomolekülextraktionssysteme und verfahren
US20040224329A1 (en) * 2003-05-08 2004-11-11 Gjerde Douglas T. Three-dimensional solid phase extraction surfaces
GB0215185D0 (en) 2002-07-01 2002-08-07 Genovision As Binding a target substance
CN1852765A (zh) * 2002-10-18 2006-10-25 普罗梅加公司 用于分离分子的组合物
US20040241721A1 (en) * 2003-05-08 2004-12-02 Gjerde Douglas T. Open channel solid phase extraction systems and methods
GB0315131D0 (en) * 2003-06-27 2003-08-06 Dynal Biotech Asa Improvements in magnetic polymer particles
WO2005000441A2 (en) 2003-06-27 2005-01-06 Dynal Biotech Asa Conjugates of magnetic polymer particles and carboxymethylated aspartic acid
US7115397B2 (en) * 2003-09-12 2006-10-03 Promega Corporation Methods and kits for purifying his-tagged proteins
WO2005037858A2 (en) * 2003-10-16 2005-04-28 University Of Massachusetts Template-directed assembly of receptor signaling complexes
GB0406015D0 (en) * 2004-03-17 2004-04-21 Dynal Biotech Asa Improvements in magnetic polymer particles
US20050272116A1 (en) * 2004-06-04 2005-12-08 Clemson University Polymer substrate for recombinant protein purification and environmental remediation
US7294614B2 (en) * 2004-10-12 2007-11-13 Clontech Laboratories, Inc. Phosphoprotein affinity resins and methods for making and using the same
US20070196833A1 (en) * 2005-04-21 2007-08-23 Gjerde Douglas T Open channel solid phase extraction systems and methods
WO2007067539A2 (en) * 2005-12-05 2007-06-14 Clontech Laboratories, Inc. High density metal ion affinity compositions and methods for making and using the same
WO2007139470A1 (en) * 2006-05-30 2007-12-06 Ge Healthcare Bio-Sciences Ab A method of preparing an immobilised metal ion chromatography adsorbent and methods of purifying proteins, peptides or polynucleotides.
WO2008002656A2 (en) * 2006-06-28 2008-01-03 P.A. Technologies Llc Methods and materials for in vitro analysis and/or use of membrane-associated proteins, portions thereof or variants thereof
EP2022561A1 (de) * 2007-08-06 2009-02-11 Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V. Immobilisierung von chelatbildenden Gruppen für immobilisierte Metallionenchromatographie (IMAC)
CN103586092B (zh) 2007-08-06 2016-06-08 马普科技促进协会 用于固定金属离子色谱法(imac)的螯合基团的固定
JP2009073819A (ja) 2007-08-29 2009-04-09 Fujifilm Corp 生理活性物質の精製方法
DE102008030142A1 (de) * 2008-06-27 2009-12-31 Qiagen Gmbh Verfahren zur Proteinaufreinigung unter denaturierenden Bedingungen
CN101323454B (zh) * 2008-07-28 2010-09-08 陕西师范大学 表面螯合金属离子的磁性二氧化硅微球制备方法
WO2010036520A1 (en) * 2008-09-26 2010-04-01 Wisconsin Alumni Research Foundation Mesoporous metal oxide materials for phosphoproteomics
BRPI1014677A2 (pt) * 2009-03-30 2016-04-12 Du Pont sistemas baseados em peptídeos para distribuição de agentes cosméticos
WO2010114638A1 (en) 2009-03-30 2010-10-07 E. I. Du Pont De Nemours And Company Peptide-based tooth whitening reagents
US8796419B2 (en) 2010-05-19 2014-08-05 Hoffmann-La Roche Inc. Hydrophobic interaction chromatography method
CN102127164B (zh) 2010-12-20 2013-01-30 武汉禾元生物科技有限公司 一种从水稻种子中提取重组人血清白蛋白的方法
CN102532254B (zh) * 2010-12-24 2015-06-24 武汉禾元生物科技股份有限公司 一种从水稻种子中分离纯化重组人血清白蛋白的方法
CN103880947B (zh) 2012-12-21 2016-01-13 武汉禾元生物科技股份有限公司 一种分离纯化高纯度重组人血清白蛋白的层析方法
ES2465715B1 (es) 2014-02-14 2015-03-18 Universitat Autónoma De Barcelona Péptido, péptido magnético y método para detectar la enfermedad celíaca
CN105085607B (zh) * 2015-09-30 2018-05-29 河南科技大学 一种利用蛋白变性分离特定金属结合蛋白的方法
CN105085606B (zh) * 2015-09-30 2018-05-29 河南科技大学 一种利用铜螯合磁珠分离铜蛋白质组的方法
EP3986466A1 (de) 2019-06-18 2022-04-27 Max-Planck-Gesellschaft zur Förderung der Wissenschaften E. V. Ortsspezifische, kinetisch inerte konjugation von markierungen und/oder trägern an zielmolekülen wie his-tag-proteine über metallkomplexreagenzien
WO2022038164A1 (en) * 2020-08-18 2022-02-24 Bia Separations D.O.O. Multimodal metal affinity processing aav capsids

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US5047513A (en) * 1986-07-10 1991-09-10 Hoffmann-La Roche Inc. Metal chelate resins
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Also Published As

Publication number Publication date
WO1998006739A1 (en) 1998-02-19
US20020143158A1 (en) 2002-10-03
DE69721289T2 (de) 2004-01-29
DE69721289D1 (de) 2003-05-28
US20060122378A1 (en) 2006-06-08
US6242581B1 (en) 2001-06-05
JP3946772B2 (ja) 2007-07-18
JP2001506968A (ja) 2001-05-29
US5962641A (en) 1999-10-05
US7193041B2 (en) 2007-03-20
EP0935606B1 (de) 2003-04-23
ES2195168T3 (es) 2003-12-01
DK0935606T3 (da) 2003-08-11
EP0935606A1 (de) 1999-08-18
WO1998006739A8 (en) 1998-05-07

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