ATE238341T1 - Verfahren zur reinigung von rekombinanaten proteinen - Google Patents
Verfahren zur reinigung von rekombinanaten proteinenInfo
- Publication number
- ATE238341T1 ATE238341T1 AT97939436T AT97939436T ATE238341T1 AT E238341 T1 ATE238341 T1 AT E238341T1 AT 97939436 T AT97939436 T AT 97939436T AT 97939436 T AT97939436 T AT 97939436T AT E238341 T1 ATE238341 T1 AT E238341T1
- Authority
- AT
- Austria
- Prior art keywords
- proteins
- recombinate
- purifying
- sup
- metal ion
- Prior art date
Links
- 238000000034 method Methods 0.000 title abstract 2
- 101000911390 Homo sapiens Coagulation factor VIII Proteins 0.000 title 1
- 102000057593 human F8 Human genes 0.000 title 1
- 229910052751 metal Inorganic materials 0.000 abstract 2
- 239000002184 metal Substances 0.000 abstract 2
- CKLJMWTZIZZHCS-REOHCLBHSA-N L-aspartic acid Chemical class OC(=O)[C@@H](N)CC(O)=O CKLJMWTZIZZHCS-REOHCLBHSA-N 0.000 abstract 1
- 102000007056 Recombinant Fusion Proteins Human genes 0.000 abstract 1
- 108010008281 Recombinant Fusion Proteins Proteins 0.000 abstract 1
- 239000003446 ligand Substances 0.000 abstract 1
- 230000003647 oxidation Effects 0.000 abstract 1
- 238000007254 oxidation reaction Methods 0.000 abstract 1
- 125000002924 primary amino group Chemical group [H]N([H])* 0.000 abstract 1
- 230000004853 protein function Effects 0.000 abstract 1
- 238000000746 purification Methods 0.000 abstract 1
- 238000012216 screening Methods 0.000 abstract 1
- 230000002194 synthesizing effect Effects 0.000 abstract 1
- 229910052723 transition metal Inorganic materials 0.000 abstract 1
- 150000003624 transition metals Chemical class 0.000 abstract 1
Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
- B01D15/08—Selective adsorption, e.g. chromatography
- B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
- B01D15/38—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving specific interaction not covered by one or more of groups B01D15/265 and B01D15/30 - B01D15/36, e.g. affinity, ligand exchange or chiral chromatography
- B01D15/3804—Affinity chromatography
- B01D15/3828—Ligand exchange chromatography, e.g. complexation, chelation or metal interaction chromatography
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
- B01J20/30—Processes for preparing, regenerating, or reactivating
- B01J20/32—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating
- B01J20/3231—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating characterised by the coating or impregnating layer
- B01J20/3242—Layers with a functional group, e.g. an affinity material, a ligand, a reactant or a complexing group
- B01J20/3244—Non-macromolecular compounds
- B01J20/3265—Non-macromolecular compounds with an organic functional group containing a metal, e.g. a metal affinity ligand
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J45/00—Ion-exchange in which a complex or a chelate is formed; Use of material as complex or chelate forming ion-exchangers; Treatment of material for improving the complex or chelate forming ion-exchange properties
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/16—Extraction; Separation; Purification by chromatography
- C07K1/22—Affinity chromatography or related techniques based upon selective absorption processes
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/43504—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates
- C07K14/43595—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates from coelenteratae, e.g. medusae
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/0004—Oxidoreductases (1.)
- C12N9/0012—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7)
- C12N9/0026—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on CH-NH groups of donors (1.5)
- C12N9/0028—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on CH-NH groups of donors (1.5) with NAD or NADP as acceptor (1.5.1)
- C12N9/003—Dihydrofolate reductase [DHFR] (1.5.1.3)
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/20—Fusion polypeptide containing a tag with affinity for a non-protein ligand
- C07K2319/21—Fusion polypeptide containing a tag with affinity for a non-protein ligand containing a His-tag
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Zoology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Analytical Chemistry (AREA)
- Medicinal Chemistry (AREA)
- Molecular Biology (AREA)
- Wood Science & Technology (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biophysics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Toxicology (AREA)
- Gastroenterology & Hepatology (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Tropical Medicine & Parasitology (AREA)
- General Engineering & Computer Science (AREA)
- Peptides Or Proteins (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Solid-Sorbent Or Filter-Aiding Compositions (AREA)
- Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US08/698,747 US5962641A (en) | 1996-08-16 | 1996-08-16 | Method for purification of recombinant proteins |
| PCT/US1997/014487 WO1998006739A1 (en) | 1996-08-16 | 1997-08-18 | Method for purification of recombinant proteins |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ATE238341T1 true ATE238341T1 (de) | 2003-05-15 |
Family
ID=24806511
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AT97939436T ATE238341T1 (de) | 1996-08-16 | 1997-08-18 | Verfahren zur reinigung von rekombinanaten proteinen |
Country Status (8)
| Country | Link |
|---|---|
| US (4) | US5962641A (de) |
| EP (1) | EP0935606B1 (de) |
| JP (1) | JP3946772B2 (de) |
| AT (1) | ATE238341T1 (de) |
| DE (1) | DE69721289T2 (de) |
| DK (1) | DK0935606T3 (de) |
| ES (1) | ES2195168T3 (de) |
| WO (1) | WO1998006739A1 (de) |
Families Citing this family (42)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2000024910A1 (en) * | 1998-10-28 | 2000-05-04 | Smithkline Beecham Plc | Process of purification of low density lipoprotein associated phospholipase a2 using immobilized metal affinity chromatography |
| US6232083B1 (en) * | 1999-03-12 | 2001-05-15 | The Research Foundation Of State University Of New York | Methods and compositions for screening cloned proteins |
| GB2367818A (en) * | 2000-10-11 | 2002-04-17 | Kalibrant Ltd | A linker and a method of chemical synthesis |
| DE10106339A1 (de) * | 2001-02-09 | 2002-08-22 | Hans-Joachim Mueller | Verfahren zum Hochdurchsatz-Screening von Kandidaten-Verbindungen über die Hochdurchsatz-Direktsynthese von rekombinanten Polypeptiden |
| US20040180415A1 (en) * | 2001-05-15 | 2004-09-16 | Tchaga Grigoriy S. | Methods and compositions for protein purification |
| US6703498B2 (en) | 2001-06-21 | 2004-03-09 | Clontech Laboratories, Inc. | Water-soluble polymeric metal ion affinity compositions and methods for using the same |
| US7151167B2 (en) * | 2002-06-10 | 2006-12-19 | Phynexus, Inc. | Open channel solid phase extraction systems and methods |
| US7122640B2 (en) * | 2002-06-10 | 2006-10-17 | Phynexus, Inc. | Open channel solid phase extraction systems and methods |
| US7879621B2 (en) * | 2003-05-08 | 2011-02-01 | Phynexus, Inc. | Open channel solid phase extraction systems and methods |
| EP1518115A2 (de) * | 2002-06-10 | 2005-03-30 | Phynexus, Inc. | Offenkanalfestphasenbiomolekülextraktionssysteme und verfahren |
| US20040224329A1 (en) * | 2003-05-08 | 2004-11-11 | Gjerde Douglas T. | Three-dimensional solid phase extraction surfaces |
| GB0215185D0 (en) | 2002-07-01 | 2002-08-07 | Genovision As | Binding a target substance |
| CN1852765A (zh) * | 2002-10-18 | 2006-10-25 | 普罗梅加公司 | 用于分离分子的组合物 |
| US20040241721A1 (en) * | 2003-05-08 | 2004-12-02 | Gjerde Douglas T. | Open channel solid phase extraction systems and methods |
| GB0315131D0 (en) * | 2003-06-27 | 2003-08-06 | Dynal Biotech Asa | Improvements in magnetic polymer particles |
| WO2005000441A2 (en) | 2003-06-27 | 2005-01-06 | Dynal Biotech Asa | Conjugates of magnetic polymer particles and carboxymethylated aspartic acid |
| US7115397B2 (en) * | 2003-09-12 | 2006-10-03 | Promega Corporation | Methods and kits for purifying his-tagged proteins |
| WO2005037858A2 (en) * | 2003-10-16 | 2005-04-28 | University Of Massachusetts | Template-directed assembly of receptor signaling complexes |
| GB0406015D0 (en) * | 2004-03-17 | 2004-04-21 | Dynal Biotech Asa | Improvements in magnetic polymer particles |
| US20050272116A1 (en) * | 2004-06-04 | 2005-12-08 | Clemson University | Polymer substrate for recombinant protein purification and environmental remediation |
| US7294614B2 (en) * | 2004-10-12 | 2007-11-13 | Clontech Laboratories, Inc. | Phosphoprotein affinity resins and methods for making and using the same |
| US20070196833A1 (en) * | 2005-04-21 | 2007-08-23 | Gjerde Douglas T | Open channel solid phase extraction systems and methods |
| WO2007067539A2 (en) * | 2005-12-05 | 2007-06-14 | Clontech Laboratories, Inc. | High density metal ion affinity compositions and methods for making and using the same |
| WO2007139470A1 (en) * | 2006-05-30 | 2007-12-06 | Ge Healthcare Bio-Sciences Ab | A method of preparing an immobilised metal ion chromatography adsorbent and methods of purifying proteins, peptides or polynucleotides. |
| WO2008002656A2 (en) * | 2006-06-28 | 2008-01-03 | P.A. Technologies Llc | Methods and materials for in vitro analysis and/or use of membrane-associated proteins, portions thereof or variants thereof |
| EP2022561A1 (de) * | 2007-08-06 | 2009-02-11 | Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V. | Immobilisierung von chelatbildenden Gruppen für immobilisierte Metallionenchromatographie (IMAC) |
| CN103586092B (zh) | 2007-08-06 | 2016-06-08 | 马普科技促进协会 | 用于固定金属离子色谱法(imac)的螯合基团的固定 |
| JP2009073819A (ja) | 2007-08-29 | 2009-04-09 | Fujifilm Corp | 生理活性物質の精製方法 |
| DE102008030142A1 (de) * | 2008-06-27 | 2009-12-31 | Qiagen Gmbh | Verfahren zur Proteinaufreinigung unter denaturierenden Bedingungen |
| CN101323454B (zh) * | 2008-07-28 | 2010-09-08 | 陕西师范大学 | 表面螯合金属离子的磁性二氧化硅微球制备方法 |
| WO2010036520A1 (en) * | 2008-09-26 | 2010-04-01 | Wisconsin Alumni Research Foundation | Mesoporous metal oxide materials for phosphoproteomics |
| BRPI1014677A2 (pt) * | 2009-03-30 | 2016-04-12 | Du Pont | sistemas baseados em peptídeos para distribuição de agentes cosméticos |
| WO2010114638A1 (en) | 2009-03-30 | 2010-10-07 | E. I. Du Pont De Nemours And Company | Peptide-based tooth whitening reagents |
| US8796419B2 (en) | 2010-05-19 | 2014-08-05 | Hoffmann-La Roche Inc. | Hydrophobic interaction chromatography method |
| CN102127164B (zh) | 2010-12-20 | 2013-01-30 | 武汉禾元生物科技有限公司 | 一种从水稻种子中提取重组人血清白蛋白的方法 |
| CN102532254B (zh) * | 2010-12-24 | 2015-06-24 | 武汉禾元生物科技股份有限公司 | 一种从水稻种子中分离纯化重组人血清白蛋白的方法 |
| CN103880947B (zh) | 2012-12-21 | 2016-01-13 | 武汉禾元生物科技股份有限公司 | 一种分离纯化高纯度重组人血清白蛋白的层析方法 |
| ES2465715B1 (es) | 2014-02-14 | 2015-03-18 | Universitat Autónoma De Barcelona | Péptido, péptido magnético y método para detectar la enfermedad celíaca |
| CN105085607B (zh) * | 2015-09-30 | 2018-05-29 | 河南科技大学 | 一种利用蛋白变性分离特定金属结合蛋白的方法 |
| CN105085606B (zh) * | 2015-09-30 | 2018-05-29 | 河南科技大学 | 一种利用铜螯合磁珠分离铜蛋白质组的方法 |
| EP3986466A1 (de) | 2019-06-18 | 2022-04-27 | Max-Planck-Gesellschaft zur Förderung der Wissenschaften E. V. | Ortsspezifische, kinetisch inerte konjugation von markierungen und/oder trägern an zielmolekülen wie his-tag-proteine über metallkomplexreagenzien |
| WO2022038164A1 (en) * | 2020-08-18 | 2022-02-24 | Bia Separations D.O.O. | Multimodal metal affinity processing aav capsids |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3853708A (en) * | 1970-01-23 | 1974-12-10 | Exploaterings Ab Tbf | Coupling biologically active substances to oxirane-containing polymers |
| US5047513A (en) * | 1986-07-10 | 1991-09-10 | Hoffmann-La Roche Inc. | Metal chelate resins |
| CA1304886C (en) * | 1986-07-10 | 1992-07-07 | Heinz Dobeli | Metal chelate resins |
-
1996
- 1996-08-16 US US08/698,747 patent/US5962641A/en not_active Expired - Lifetime
-
1997
- 1997-08-18 DK DK97939436T patent/DK0935606T3/da active
- 1997-08-18 WO PCT/US1997/014487 patent/WO1998006739A1/en not_active Ceased
- 1997-08-18 ES ES97939436T patent/ES2195168T3/es not_active Expired - Lifetime
- 1997-08-18 EP EP97939436A patent/EP0935606B1/de not_active Expired - Lifetime
- 1997-08-18 AT AT97939436T patent/ATE238341T1/de not_active IP Right Cessation
- 1997-08-18 US US08/912,406 patent/US6242581B1/en not_active Expired - Lifetime
- 1997-08-18 DE DE69721289T patent/DE69721289T2/de not_active Expired - Lifetime
- 1997-08-18 JP JP51009798A patent/JP3946772B2/ja not_active Expired - Lifetime
-
2001
- 2001-04-19 US US09/839,696 patent/US7193041B2/en not_active Expired - Lifetime
-
2006
- 2006-01-19 US US11/336,748 patent/US20060122378A1/en not_active Abandoned
Also Published As
| Publication number | Publication date |
|---|---|
| WO1998006739A1 (en) | 1998-02-19 |
| US20020143158A1 (en) | 2002-10-03 |
| DE69721289T2 (de) | 2004-01-29 |
| DE69721289D1 (de) | 2003-05-28 |
| US20060122378A1 (en) | 2006-06-08 |
| US6242581B1 (en) | 2001-06-05 |
| JP3946772B2 (ja) | 2007-07-18 |
| JP2001506968A (ja) | 2001-05-29 |
| US5962641A (en) | 1999-10-05 |
| US7193041B2 (en) | 2007-03-20 |
| EP0935606B1 (de) | 2003-04-23 |
| ES2195168T3 (es) | 2003-12-01 |
| DK0935606T3 (da) | 2003-08-11 |
| EP0935606A1 (de) | 1999-08-18 |
| WO1998006739A8 (en) | 1998-05-07 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| RER | Ceased as to paragraph 5 lit. 3 law introducing patent treaties |