ATE245694T1 - Methode zur erhöhung der effizienz gerichteter evolution eines gens mittels phagemiden - Google Patents
Methode zur erhöhung der effizienz gerichteter evolution eines gens mittels phagemidenInfo
- Publication number
- ATE245694T1 ATE245694T1 AT98956454T AT98956454T ATE245694T1 AT E245694 T1 ATE245694 T1 AT E245694T1 AT 98956454 T AT98956454 T AT 98956454T AT 98956454 T AT98956454 T AT 98956454T AT E245694 T1 ATE245694 T1 AT E245694T1
- Authority
- AT
- Austria
- Prior art keywords
- gene
- phagemid
- host cell
- phagemids
- efficiency
- Prior art date
Links
- 238000000034 method Methods 0.000 title abstract 4
- 108090000623 proteins and genes Proteins 0.000 title abstract 3
- 230000002068 genetic effect Effects 0.000 abstract 1
- 230000035772 mutation Effects 0.000 abstract 1
- 230000000869 mutational effect Effects 0.000 abstract 1
- 238000004806 packaging method and process Methods 0.000 abstract 1
- 238000012216 screening Methods 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/01—Preparation of mutants without inserting foreign genetic material therein; Screening processes therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/102—Mutagenizing nucleic acids
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/78—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5)
- C12N9/86—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5) acting on amide bonds in cyclic amides, e.g. penicillinase (3.5.2)
Landscapes
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Plant Pathology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Medicinal Chemistry (AREA)
- Crystallography & Structural Chemistry (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Enzymes And Modification Thereof (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US08/968,627 US6156509A (en) | 1997-11-12 | 1997-11-12 | Method of increasing efficiency of directed evolution of a gene using phagemid |
| PCT/US1998/023278 WO1999024562A1 (en) | 1997-11-12 | 1998-11-02 | Method of increasing efficiency of directed evolution of a gene using phagemid |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ATE245694T1 true ATE245694T1 (de) | 2003-08-15 |
Family
ID=25514522
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AT98956454T ATE245694T1 (de) | 1997-11-12 | 1998-11-02 | Methode zur erhöhung der effizienz gerichteter evolution eines gens mittels phagemiden |
Country Status (10)
| Country | Link |
|---|---|
| US (1) | US6156509A (de) |
| EP (1) | EP1029040B1 (de) |
| JP (1) | JP2001522602A (de) |
| AT (1) | ATE245694T1 (de) |
| AU (1) | AU737419B2 (de) |
| CA (1) | CA2309207A1 (de) |
| DE (1) | DE69816647T2 (de) |
| DK (1) | DK1029040T3 (de) |
| NZ (1) | NZ504198A (de) |
| WO (1) | WO1999024562A1 (de) |
Families Citing this family (52)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6696278B1 (en) * | 2001-02-26 | 2004-02-24 | Stratagene | Method for transfer of DNA segments |
| US7647184B2 (en) * | 2001-08-27 | 2010-01-12 | Hanall Pharmaceuticals, Co. Ltd | High throughput directed evolution by rational mutagenesis |
| US20030224404A1 (en) * | 2002-02-25 | 2003-12-04 | Manuel Vega | High throughput directed evolution of nucleic acids by rational mutagenesis |
| US20060020396A1 (en) * | 2002-09-09 | 2006-01-26 | Rene Gantier | Rational directed protein evolution using two-dimensional rational mutagenesis scanning |
| DE60332358D1 (de) * | 2002-09-09 | 2010-06-10 | Hanall Pharmaceutical Co Ltd | Protease-resistente modifizierte interferon alpha polypeptide |
| US20050202438A1 (en) * | 2002-09-09 | 2005-09-15 | Rene Gantier | Rational directed protein evolution using two-dimensional rational mutagenesis scanning |
| US20040248109A1 (en) * | 2003-06-09 | 2004-12-09 | Lawrence Greenfield | Methods for selecting protein binding moieties |
| WO2010028347A2 (en) | 2008-09-05 | 2010-03-11 | President & Fellows Of Harvard College | Continuous directed evolution of proteins and nucleic acids |
| CA2825370A1 (en) | 2010-12-22 | 2012-06-28 | President And Fellows Of Harvard College | Continuous directed evolution |
| US10323236B2 (en) | 2011-07-22 | 2019-06-18 | President And Fellows Of Harvard College | Evaluation and improvement of nuclease cleavage specificity |
| US9163284B2 (en) | 2013-08-09 | 2015-10-20 | President And Fellows Of Harvard College | Methods for identifying a target site of a Cas9 nuclease |
| WO2015035168A1 (en) * | 2013-09-05 | 2015-03-12 | Massachusetts Institute Of Technolgy | Tuning bacteriophage host range |
| US9322037B2 (en) | 2013-09-06 | 2016-04-26 | President And Fellows Of Harvard College | Cas9-FokI fusion proteins and uses thereof |
| US9228207B2 (en) | 2013-09-06 | 2016-01-05 | President And Fellows Of Harvard College | Switchable gRNAs comprising aptamers |
| WO2015134121A2 (en) | 2014-01-20 | 2015-09-11 | President And Fellows Of Harvard College | Negative selection and stringency modulation in continuous evolution systems |
| US10077453B2 (en) | 2014-07-30 | 2018-09-18 | President And Fellows Of Harvard College | CAS9 proteins including ligand-dependent inteins |
| WO2016077052A2 (en) | 2014-10-22 | 2016-05-19 | President And Fellows Of Harvard College | Evolution of proteases |
| WO2016168631A1 (en) | 2015-04-17 | 2016-10-20 | President And Fellows Of Harvard College | Vector-based mutagenesis system |
| WO2017015545A1 (en) | 2015-07-22 | 2017-01-26 | President And Fellows Of Harvard College | Evolution of site-specific recombinases |
| US11524983B2 (en) | 2015-07-23 | 2022-12-13 | President And Fellows Of Harvard College | Evolution of Bt toxins |
| WO2017019895A1 (en) | 2015-07-30 | 2017-02-02 | President And Fellows Of Harvard College | Evolution of talens |
| IL310721B2 (en) | 2015-10-23 | 2025-11-01 | Harvard College | Nucleobase editors and uses thereof |
| CN110214183A (zh) | 2016-08-03 | 2019-09-06 | 哈佛大学的校长及成员们 | 腺苷核碱基编辑器及其用途 |
| WO2018031683A1 (en) | 2016-08-09 | 2018-02-15 | President And Fellows Of Harvard College | Programmable cas9-recombinase fusion proteins and uses thereof |
| US11542509B2 (en) | 2016-08-24 | 2023-01-03 | President And Fellows Of Harvard College | Incorporation of unnatural amino acids into proteins using base editing |
| KR102622411B1 (ko) | 2016-10-14 | 2024-01-10 | 프레지던트 앤드 펠로우즈 오브 하바드 칼리지 | 핵염기 에디터의 aav 전달 |
| WO2018119359A1 (en) | 2016-12-23 | 2018-06-28 | President And Fellows Of Harvard College | Editing of ccr5 receptor gene to protect against hiv infection |
| CN110249049A (zh) * | 2016-12-29 | 2019-09-17 | 法兰克福大学 | 产生高阶基因组编辑文库的方法 |
| EP3592853A1 (de) | 2017-03-09 | 2020-01-15 | President and Fellows of Harvard College | Unterdrückung von schmerzen durch geneditierung |
| US12390514B2 (en) | 2017-03-09 | 2025-08-19 | President And Fellows Of Harvard College | Cancer vaccine |
| US11542496B2 (en) | 2017-03-10 | 2023-01-03 | President And Fellows Of Harvard College | Cytosine to guanine base editor |
| KR20240116572A (ko) | 2017-03-23 | 2024-07-29 | 프레지던트 앤드 펠로우즈 오브 하바드 칼리지 | 핵산 프로그램가능한 dna 결합 단백질을 포함하는 핵염기 편집제 |
| US11560566B2 (en) | 2017-05-12 | 2023-01-24 | President And Fellows Of Harvard College | Aptazyme-embedded guide RNAs for use with CRISPR-Cas9 in genome editing and transcriptional activation |
| US11447809B2 (en) | 2017-07-06 | 2022-09-20 | President And Fellows Of Harvard College | Evolution of tRNA synthetases |
| CN111801345A (zh) | 2017-07-28 | 2020-10-20 | 哈佛大学的校长及成员们 | 使用噬菌体辅助连续进化(pace)的进化碱基编辑器的方法和组合物 |
| US12060553B2 (en) | 2017-08-25 | 2024-08-13 | President And Fellows Of Harvard College | Evolution of BoNT peptidases |
| EP3676376B1 (de) | 2017-08-30 | 2025-01-15 | President and Fellows of Harvard College | Hocheffiziente baseneditoren mit gam |
| WO2019056002A1 (en) | 2017-09-18 | 2019-03-21 | President And Fellows Of Harvard College | CONTINUOUS EVOLUTION FOR STABILIZED PROTEINS |
| KR20250107288A (ko) | 2017-10-16 | 2025-07-11 | 더 브로드 인스티튜트, 인코퍼레이티드 | 아데노신 염기 편집제의 용도 |
| US12406749B2 (en) | 2017-12-15 | 2025-09-02 | The Broad Institute, Inc. | Systems and methods for predicting repair outcomes in genetic engineering |
| US12157760B2 (en) | 2018-05-23 | 2024-12-03 | The Broad Institute, Inc. | Base editors and uses thereof |
| WO2019241649A1 (en) | 2018-06-14 | 2019-12-19 | President And Fellows Of Harvard College | Evolution of cytidine deaminases |
| US12522807B2 (en) | 2018-07-09 | 2026-01-13 | The Broad Institute, Inc. | RNA programmable epigenetic RNA modifiers and uses thereof |
| WO2020092453A1 (en) | 2018-10-29 | 2020-05-07 | The Broad Institute, Inc. | Nucleobase editors comprising geocas9 and uses thereof |
| US12351837B2 (en) | 2019-01-23 | 2025-07-08 | The Broad Institute, Inc. | Supernegatively charged proteins and uses thereof |
| WO2020191233A1 (en) | 2019-03-19 | 2020-09-24 | The Broad Institute, Inc. | Methods and compositions for editing nucleotide sequences |
| US12473543B2 (en) | 2019-04-17 | 2025-11-18 | The Broad Institute, Inc. | Adenine base editors with reduced off-target effects |
| US12435330B2 (en) | 2019-10-10 | 2025-10-07 | The Broad Institute, Inc. | Methods and compositions for prime editing RNA |
| IL297761A (en) | 2020-05-08 | 2022-12-01 | Broad Inst Inc | Methods and compositions for simultaneously editing two helices of a designated double-helix nucleotide sequence |
| CN114854781A (zh) * | 2022-03-22 | 2022-08-05 | 翌圣生物科技(上海)股份有限公司 | Ap质粒及其应用、pap i酶的pace定向进化系统及pap i酶突变体 |
| CN116162616A (zh) * | 2023-01-28 | 2023-05-26 | 态创生物科技(广州)有限公司 | 高效诱变方法在提高噬菌体辅助进化效率中的应用 |
| DE102023001827A1 (de) | 2023-04-19 | 2024-10-24 | Horst Wochnowski | Verfahren und dazugehörige Vorrichtung zum Züchten von in Gewässern Mikroplastik zersetzenden und abbauenden Mikroorganismenstämme durch einen künstlich herbeigeführten und beschleunigten Evolutions- und Selektionsprozess |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4980288A (en) * | 1986-02-12 | 1990-12-25 | Genex Corporation | Subtilisin with increased thermal stability |
| US5844094A (en) * | 1992-09-25 | 1998-12-01 | Commonwealth Scientific And Industrial Research Organization | Target binding polypeptide |
| US6117679A (en) * | 1994-02-17 | 2000-09-12 | Maxygen, Inc. | Methods for generating polynucleotides having desired characteristics by iterative selection and recombination |
| US5521077A (en) * | 1994-04-28 | 1996-05-28 | The Leland Stanford Junior University | Method of generating multiple protein variants and populations of protein variants prepared thereby |
-
1997
- 1997-11-12 US US08/968,627 patent/US6156509A/en not_active Expired - Lifetime
-
1998
- 1998-11-02 DK DK98956454T patent/DK1029040T3/da active
- 1998-11-02 AT AT98956454T patent/ATE245694T1/de not_active IP Right Cessation
- 1998-11-02 JP JP2000520558A patent/JP2001522602A/ja active Pending
- 1998-11-02 WO PCT/US1998/023278 patent/WO1999024562A1/en not_active Ceased
- 1998-11-02 DE DE69816647T patent/DE69816647T2/de not_active Expired - Lifetime
- 1998-11-02 CA CA002309207A patent/CA2309207A1/en not_active Abandoned
- 1998-11-02 NZ NZ504198A patent/NZ504198A/en unknown
- 1998-11-02 AU AU12977/99A patent/AU737419B2/en not_active Ceased
- 1998-11-02 EP EP98956454A patent/EP1029040B1/de not_active Expired - Lifetime
Also Published As
| Publication number | Publication date |
|---|---|
| EP1029040A1 (de) | 2000-08-23 |
| DK1029040T3 (da) | 2003-11-17 |
| US6156509A (en) | 2000-12-05 |
| CA2309207A1 (en) | 1999-05-20 |
| DE69816647T2 (de) | 2004-04-01 |
| EP1029040B1 (de) | 2003-07-23 |
| WO1999024562A1 (en) | 1999-05-20 |
| NZ504198A (en) | 2001-11-30 |
| DE69816647D1 (de) | 2003-08-28 |
| AU1297799A (en) | 1999-05-31 |
| JP2001522602A (ja) | 2001-11-20 |
| AU737419B2 (en) | 2001-08-16 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| ATE245694T1 (de) | Methode zur erhöhung der effizienz gerichteter evolution eines gens mittels phagemiden | |
| Haridas et al. | 101 Dothideomycetes genomes: a test case for predicting lifestyles and emergence of pathogens | |
| Carlier et al. | Septoria leaf spot of banana: a newly discovered disease caused by Mycosphaerella eumusae (anamorph Septoria eumusae) | |
| Sloan | One ring to rule them all? Genome sequencing provides new insights into the ‘master circle’model of plant mitochondrial DNA structure | |
| Bossi | Context effects: translation of UAG codon by suppressor tRNA is affected by the sequence following UAG in the message | |
| Rohwer et al. | The complete genomic sequence of the marine phage Roseophage SIO1 shares homology with nonmarine phages | |
| DE69932345D1 (de) | Erstellung und durchmusterung von interessierenden dna-banken in zellen von filamentösen pilzen | |
| Dyson et al. | Lysis to kill: evaluation of the lytic abilities, and genomics of nine bacteriophages infective for Gordonia spp. and their potential use in activated sludge foam biocontrol | |
| WO2004031361A3 (en) | Three-dimensional peripheral lymphoid organ cell cultures | |
| WO2003074734A3 (en) | Methods for detecting genome-wide sequence variations associated with a phenotype | |
| ES2255281T3 (es) | Sobreexpresion de genes de fitasa en sistmas de levadura. | |
| Maniloff et al. | Mycoplasma viruses | |
| Adler et al. | Genome-wide characterization of diverse bacteriophages enabled by RNA-binding CRISPRi | |
| Comer et al. | An ochre suppressor of bacteriophage T4 that is associated with a transfer RNA | |
| Beakes et al. | Esterase isoenzyme variation in the genus Saprolegnia, with particular reference to the fish-pathogenic S. diclina-parasitica complex | |
| Piel et al. | Genetic determinism of phage-bacteria coevolution in natural populations | |
| Ford et al. | Introducing a novel, broad host range temperate phage family infecting Rhizobium leguminosarum and beyond | |
| Battilani et al. | Quasispecies composition and phylogenetic analysis of feline coronaviruses (FCoVs) in naturally infected cats | |
| CN103757053A (zh) | 一种特异性的dna病毒基因组定点改造及筛选方法 | |
| Ren et al. | Ligilactobacillus cholophilus sp. nov., isolated from pickled potherb mustard (Brassica juncea Coss.) | |
| Du et al. | Host and geographic range extensions of Melanconiella, with a new species M. cornuta in China | |
| Szafranski et al. | Non-LTR retrotransposons with unique integration preferences downstream of Dictyostelium discoideum tRNA genes | |
| McAuslan et al. | The biochemistry of icosahedral cytoplasmic deoxyviruses | |
| Skinner et al. | Differentiation between types and strains of Clostridium botulinum by riboprinting | |
| Chung et al. | Antimicrobial susceptibilities and epidemiological analysis of Salmonella enteritidis isolates in Korea by phage typing and pulsed-field gel electrophoresis |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| RER | Ceased as to paragraph 5 lit. 3 law introducing patent treaties |