ATE247167T1 - Methode zur kontrollierten herstellung von polypeptiden in bakterien - Google Patents

Methode zur kontrollierten herstellung von polypeptiden in bakterien

Info

Publication number
ATE247167T1
ATE247167T1 AT94901629T AT94901629T ATE247167T1 AT E247167 T1 ATE247167 T1 AT E247167T1 AT 94901629 T AT94901629 T AT 94901629T AT 94901629 T AT94901629 T AT 94901629T AT E247167 T1 ATE247167 T1 AT E247167T1
Authority
AT
Austria
Prior art keywords
nucleic acid
polypeptide
phosphate
psts
promoter
Prior art date
Application number
AT94901629T
Other languages
English (en)
Inventor
Steven Bass
James R Swartz
Original Assignee
Genentech Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Genentech Inc filed Critical Genentech Inc
Application granted granted Critical
Publication of ATE247167T1 publication Critical patent/ATE247167T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/24—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Enterobacteriaceae (F), e.g. Citrobacter, Serratia, Proteus, Providencia, Morganella, Yersinia
    • C07K14/245—Escherichia (G)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/65—Insulin-like growth factors, i.e. somatomedins, e.g. IGF-1, IGF-2
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/38—Chemical stimulation of growth or activity by addition of chemical compounds which are not essential growth factors; Stimulation of growth by removal of a chemical compound
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biophysics (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Toxicology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Endocrinology (AREA)
  • Diabetes (AREA)
  • General Chemical & Material Sciences (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
AT94901629T 1992-11-20 1993-11-19 Methode zur kontrollierten herstellung von polypeptiden in bakterien ATE247167T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US07/989,845 US5304472A (en) 1992-11-20 1992-11-20 Method of controlling polypeptide production in bacterial cells
PCT/US1993/011298 WO1994012636A1 (en) 1992-11-20 1993-11-19 Method of controlling polypeptide production in bacteria

Publications (1)

Publication Number Publication Date
ATE247167T1 true ATE247167T1 (de) 2003-08-15

Family

ID=25535525

Family Applications (1)

Application Number Title Priority Date Filing Date
AT94901629T ATE247167T1 (de) 1992-11-20 1993-11-19 Methode zur kontrollierten herstellung von polypeptiden in bakterien

Country Status (12)

Country Link
US (1) US5304472A (de)
EP (1) EP0669980B1 (de)
JP (1) JP3503705B2 (de)
AT (1) ATE247167T1 (de)
AU (1) AU671512B2 (de)
CA (1) CA2145388C (de)
DE (1) DE69333145T2 (de)
DK (1) DK0669980T3 (de)
ES (1) ES2204911T3 (de)
IL (1) IL107564A (de)
PT (1) PT669980E (de)
WO (1) WO1994012636A1 (de)

Families Citing this family (32)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5789199A (en) * 1994-11-03 1998-08-04 Genentech, Inc. Process for bacterial production of polypeptides
US6140305A (en) * 1996-04-04 2000-10-31 Bio-Rad Laboratories, Inc. Hereditary hemochromatosis gene products
US6027888A (en) * 1996-04-05 2000-02-22 Board Of Regents, The University Of Texas System Methods for producing soluble, biologically-active disulfide-bond containing eukaryotic proteins in bacterial cells
CN1244214A (zh) * 1996-11-08 2000-02-09 尼澳斯技术股份有限公司 改进的表达载体
US6083715A (en) * 1997-06-09 2000-07-04 Board Of Regents, The University Of Texas System Methods for producing heterologous disulfide bond-containing polypeptides in bacterial cells
AU2487300A (en) 1998-12-31 2000-07-31 Chiron Corporation Polynucleotides encoding antigenic hiv type c polypeptides, polypeptides and uses thereof
JP4776075B2 (ja) * 1998-12-31 2011-09-21 ノバルティス バクシンズ アンド ダイアグノスティックス，インコーポレーテッド 改変ｈｉｖｅｎｖポリペプチド
US7935805B1 (en) * 1998-12-31 2011-05-03 Novartis Vaccines & Diagnostics, Inc Polynucleotides encoding antigenic HIV Type C polypeptides, polypeptides and uses thereof
CA2360347C (en) 1998-12-31 2013-05-07 Chiron Corporation Improved expression of hiv polypeptides and production of virus-like particles
DE10012283A1 (de) * 2000-03-14 2001-09-20 Thomas Schweder Wirts-Vektor-Systeme zur phosphatregulierten Überproduktion von Polypeptiden in Becillus
WO2003004657A1 (en) * 2001-07-05 2003-01-16 Chiron Corporation Polynucleotides encoding antigenic hiv type b and/or type c polypeptides, polypeptides and uses thereof
AU2002320314A1 (en) 2001-07-05 2003-01-21 Chiron, Corporation Polynucleotides encoding antigenic hiv type c polypeptides, polypeptides and uses thereof
WO2003054140A2 (en) 2001-07-30 2003-07-03 Genencor International, Inc. Phosphate limited inducible promoter and a bacillus expression system
US20030170614A1 (en) * 2001-08-31 2003-09-11 Megede Jan Zur Polynucleotides encoding antigenic HIV type B polypeptides, polypeptides and uses thereof
WO2003020876A2 (en) 2001-08-31 2003-03-13 Chiron Corporation Polynucleotides encoding antigenic hiv type b polypeptides, polypeptides and uses thereof
HU225243B1 (en) * 2001-09-13 2006-08-28 Genentech Inc Gram-negative bacterium cell containing a gene coding an aminopeptidase
GB0224082D0 (en) * 2002-10-16 2002-11-27 Celltech R&D Ltd Biological products
WO2004081033A2 (en) * 2003-03-11 2004-09-23 National Institutes Of Health Synthesis of proteins by cell-free protein expression
ZA200606913B (en) * 2004-03-11 2008-03-26 Genentech Inc Process for producing polypeptides
AU2012203817B2 (en) * 2004-03-11 2015-05-28 Genentech, Inc. Process for producing polypeptides
JP2008525041A (ja) 2004-12-22 2008-07-17 ジェネンテック・インコーポレーテッド 可溶性多膜貫通型タンパク質の産生方法
NZ568809A (en) 2005-12-22 2011-08-26 Genentech Inc Recovering and purification of VEGF proteins from prokaryotic cells using polyanionic agents
US20080213811A1 (en) * 2006-08-11 2008-09-04 Invitrogen Corporation Sensor proteins and assay methods
GB201000591D0 (en) 2010-01-14 2010-03-03 Ucb Pharma Sa Bacterial hoist strain
GB201000590D0 (en) 2010-01-14 2010-03-03 Ucb Pharma Sa Bacterial host strain
GB201000587D0 (en) 2010-01-14 2010-03-03 Ucb Pharma Sa Bacterial hoist strain
GB201000588D0 (en) 2010-01-14 2010-03-03 Ucb Pharma Sa Bacterial host strain
EP2546267A1 (de) 2011-07-13 2013-01-16 UCB Pharma S.A. Bakterienwirtsstamm mit rekombinanter DsbC-Expression
PL2731973T3 (pl) 2011-07-13 2018-04-30 Ucb Biopharma Sprl Bakteryjny szczep gospodarz wykazujący ekspresję dsbc
GB201208367D0 (en) 2012-05-14 2012-06-27 Ucb Pharma Sa Biological product
WO2014159850A2 (en) * 2013-03-14 2014-10-02 Codexis, Inc. Low-phosphate repressible promoter
KR102185850B1 (ko) 2020-02-21 2020-12-02 씨제이제일제당 주식회사 퓨린 뉴클레오티드를 생산하는 미생물 및 이를 이용한 퓨린 뉴클레오티드의 생산방법

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS592689A (ja) * 1982-06-04 1984-01-09 Handai Biseibutsubiyou Kenkyukai 強力な遺伝子発現能を有する新規レプリコンの作成法
FR2575760A1 (fr) * 1985-01-04 1986-07-11 Merieux Inst Nouvelles souches d'escherichia coli excretrices de phosphatase alcaline, procede d'obtention de ces souches, procede de production de phosphatase alcaline et de proteines hybrides derivees
JPS61280292A (ja) * 1985-06-04 1986-12-10 Wakunaga Pharmaceut Co Ltd 菌体外分泌による蛋白質の製造法
FR2599380A1 (fr) * 1986-05-29 1987-12-04 Centre Nat Rech Scient Vecteur d'exportation de proteines chez escherichiacoli, bacteries transformees et procede pour la preparation de proteines
FR2606031A1 (fr) * 1986-11-03 1988-05-06 Merieux Inst Nouvelles souches d'escherichia coli hyperproductrices et/ou hyperexcretrices de proteines exportees, procede d'obtention de ces souches, et application a la production de proteines
EP0441252B1 (de) * 1990-02-07 1997-09-03 Abbott Laboratories Alkalische Phosphatase mit verbesserter spezifischer Aktivität zur Verwendung in Indikatorreagenzien

Also Published As

Publication number Publication date
CA2145388A1 (en) 1994-06-09
ES2204911T3 (es) 2004-05-01
IL107564A0 (en) 1994-02-27
DK0669980T3 (da) 2003-11-24
JPH08503612A (ja) 1996-04-23
WO1994012636A1 (en) 1994-06-09
US5304472A (en) 1994-04-19
EP0669980B1 (de) 2003-08-13
IL107564A (en) 2000-08-31
PT669980E (pt) 2003-12-31
JP3503705B2 (ja) 2004-03-08
EP0669980A1 (de) 1995-09-06
CA2145388C (en) 2004-08-10
AU671512B2 (en) 1996-08-29
AU5615194A (en) 1994-06-22
DE69333145D1 (de) 2003-09-18
DE69333145T2 (de) 2004-06-17

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