ATE255632T1 - Immortalisierung bzw. desimmortalisierung von zellen - Google Patents

Immortalisierung bzw. desimmortalisierung von zellen

Info

Publication number
ATE255632T1
ATE255632T1 AT96918405T AT96918405T ATE255632T1 AT E255632 T1 ATE255632 T1 AT E255632T1 AT 96918405 T AT96918405 T AT 96918405T AT 96918405 T AT96918405 T AT 96918405T AT E255632 T1 ATE255632 T1 AT E255632T1
Authority
AT
Austria
Prior art keywords
cells
immortalization
disimmertalization
compositions
methods
Prior art date
Application number
AT96918405T
Other languages
English (en)
Inventor
David J Anderson
Original Assignee
California Inst Of Techn
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by California Inst Of Techn filed Critical California Inst Of Techn
Application granted granted Critical
Publication of ATE255632T1 publication Critical patent/ATE255632T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/86—Viral vectors
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • A61K38/16—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • A61K38/162—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from virus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
    • C12N15/907—Stable introduction of foreign DNA into chromosome using homologous recombination in mammalian cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K48/00—Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2740/00—Reverse transcribing RNA viruses
    • C12N2740/00011—Details
    • C12N2740/10011—Retroviridae
    • C12N2740/10041—Use of virus, viral particle or viral elements as a vector
    • C12N2740/10043—Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2740/00—Reverse transcribing RNA viruses
    • C12N2740/00011—Details
    • C12N2740/10011—Retroviridae
    • C12N2740/13011—Gammaretrovirus, e.g. murine leukeamia virus
    • C12N2740/13041—Use of virus, viral particle or viral elements as a vector
    • C12N2740/13043—Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2800/00—Nucleic acids vectors
    • C12N2800/30—Vector systems comprising sequences for excision in presence of a recombinase, e.g. loxP or FRT
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2840/00—Vectors comprising a special translation-regulating system
    • C12N2840/20—Vectors comprising a special translation-regulating system translation of more than one cistron
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2840/00—Vectors comprising a special translation-regulating system
    • C12N2840/20—Vectors comprising a special translation-regulating system translation of more than one cistron
    • C12N2840/203—Vectors comprising a special translation-regulating system translation of more than one cistron having an IRES

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Virology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Mycology (AREA)
  • Cell Biology (AREA)
  • Veterinary Medicine (AREA)
  • Public Health (AREA)
  • Animal Behavior & Ethology (AREA)
  • Epidemiology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Immunology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
AT96918405T 1995-06-07 1996-06-06 Immortalisierung bzw. desimmortalisierung von zellen ATE255632T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US08/481,127 US5629159A (en) 1995-06-07 1995-06-07 Immortalization and disimmortalization of cells
PCT/US1996/009781 WO1996040877A1 (en) 1995-06-07 1996-06-06 Immortalization and disimmortalization of cells

Publications (1)

Publication Number Publication Date
ATE255632T1 true ATE255632T1 (de) 2003-12-15

Family

ID=23910719

Family Applications (1)

Application Number Title Priority Date Filing Date
AT96918405T ATE255632T1 (de) 1995-06-07 1996-06-06 Immortalisierung bzw. desimmortalisierung von zellen

Country Status (10)

Country Link
US (1) US5629159A (de)
EP (1) EP0832196B1 (de)
JP (1) JP3875267B2 (de)
AT (1) ATE255632T1 (de)
AU (1) AU700690B2 (de)
CA (1) CA2222425C (de)
DE (1) DE69630955T2 (de)
ES (1) ES2211959T3 (de)
NO (1) NO323174B1 (de)
WO (1) WO1996040877A1 (de)

Families Citing this family (25)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20030202967A1 (en) * 1995-02-10 2003-10-30 The Regents Of The University Of California Development and use of human pancreatic cell lines
US6110743A (en) * 1995-02-10 2000-08-29 The Regents Of The University Of California Development and use of human pancreatic cell lines
FR2737731B1 (fr) * 1995-08-07 1997-10-10 Pasteur Institut Sequence de retroelements naturels ou synthetiques ayant pour fonction de permettre l'insertion de sequences nucleotidiques dans une cellule eucaryote
WO1998027217A1 (en) 1996-12-16 1998-06-25 Eisai Co., Ltd. Method for preparing retrovirus vector for gene therapy
WO1998029533A1 (en) * 1996-12-31 1998-07-09 Gaitanaris George A Vectors and methods for the mutagenesis of mammalian genes
DE19740571C1 (de) * 1997-09-15 1999-03-18 Gsf Forschungszentrum Umwelt Verfahren zur Stimulation von T-Zellen mit gewünschter Antigenspezifität
US6897066B1 (en) * 1997-09-26 2005-05-24 Athersys, Inc. Compositions and methods for non-targeted activation of endogenous genes
AU1284999A (en) * 1997-10-28 1999-05-17 Jain, Mukesh (in vitro) differentiation of vascular smooth muscle cells, methods and reagentsrelated thereto
DE19816116C2 (de) * 1998-04-09 2000-06-29 Gsf Forschungszentrum Umwelt Verfahren zur Immortalisation von Zellen mit Hilfe konditional transformierter Helferzellen
WO2000018239A1 (en) * 1998-09-25 2000-04-06 University Of Nebraska Board Of Regents Reversibly immortalized hepatocytes and methods of use
EP1144597A2 (de) * 1999-01-21 2001-10-17 Vitro Diagnostics, Inc. Immortalisierte zelllinien und deren herstellungsverfahren
US6890726B1 (en) 1999-04-06 2005-05-10 Oklahoma Medical Research Foundation Method for selecting recombinase variants with altered specificity
EP1103615A1 (de) * 1999-11-25 2001-05-30 Universite De Geneve Vektoren, die nicht-teilende Zellen immortalisieren können und Zellen, die mit diesen Vektoren immortalisiert wurden
US20040231006A1 (en) * 2000-04-12 2004-11-18 Silver Daniel P. Self-extinguishing recombinases, nucleic acids encoding them and methods of using the same
WO2001079471A2 (en) 2000-04-12 2001-10-25 Dana-Farber Cancer Institute Self-extinguishing recombinases, nucleic acids encoding them and methods of using the same
FR2810046B1 (fr) * 2000-06-07 2004-09-10 Neurotech Procede d'immortalisation conditionnelle de cellules et les constructions proteiques pour sa mise en oeuvre
JP4185264B2 (ja) * 2001-07-02 2008-11-26 富士フイルム株式会社 偏光方向制御素子及び露光装置
JP2003033179A (ja) * 2001-07-05 2003-02-04 Asahi Kasei Corp 可逆的遺伝子導入ベクター
US6645763B2 (en) * 2001-10-12 2003-11-11 Naoya Kobayashi Immortalized bone marrow mesenchymal stem cell
GB0300208D0 (en) * 2003-01-06 2003-02-05 Oxford Biomedica Ltd Insulin producing cells
AU2003267969A1 (en) * 2003-04-17 2004-11-26 U.S. Department Of Veterans Affairs Isolated/cloned human nt2 cell lines expressing serotonin and gaba
GB0316882D0 (en) * 2003-07-18 2003-08-20 Consejo Superior Investigacion Reversible immortalization of OEG from human olfactory bulbs as a tool to promote spinal cord regeneration
CA2584738A1 (en) * 2004-10-20 2006-04-27 Vitro Diagnostics, Inc. Generation and differentiation of adult stem cell lines
US20070093439A1 (en) * 2005-10-25 2007-04-26 Idera Pharmaceuticals, Inc. Short immunomodulatory oligonucleotides
CN113355354A (zh) * 2020-03-03 2021-09-07 杭州启函生物科技有限公司 控制细胞行为的系统和方法

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA1293460C (en) * 1985-10-07 1991-12-24 Brian Lee Sauer Site-specific recombination of dna in yeast
US5399346A (en) * 1989-06-14 1995-03-21 The United States Of America As Represented By The Department Of Health And Human Services Gene therapy
WO1992015694A1 (en) * 1991-03-08 1992-09-17 The Salk Institute For Biological Studies Flp-mediated gene modification in mammalian cells, and compositions and cells useful therefor
WO1993001283A1 (en) * 1991-07-08 1993-01-21 The United States Of America As Represented By The Secretary Of Agriculture Selection-gene-free transgenic plants
AU4115693A (en) * 1992-04-24 1993-11-29 Sri International In vivo homologous sequence targeting in eukaryotic cells
NZ256154A (en) * 1992-07-27 1997-02-24 California Inst Of Techn Production of mammalian multipotent neural stem cells, antibodies
US5387237A (en) * 1992-07-30 1995-02-07 The University Of Toledo Bioartificial pancreas

Also Published As

Publication number Publication date
NO975557L (no) 1998-02-05
US5629159A (en) 1997-05-13
JP3875267B2 (ja) 2007-01-31
ES2211959T3 (es) 2004-07-16
CA2222425A1 (en) 1996-12-19
NO975557D0 (no) 1997-12-02
JPH11507230A (ja) 1999-06-29
DE69630955T2 (de) 2004-10-21
NO323174B1 (no) 2007-01-15
WO1996040877A1 (en) 1996-12-19
DE69630955D1 (de) 2004-01-15
AU700690B2 (en) 1999-01-14
CA2222425C (en) 2007-08-21
AU6107796A (en) 1996-12-30
EP0832196B1 (de) 2003-12-03
EP0832196A1 (de) 1998-04-01

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