ATE256749T1 - Dna-integration durch transposition - Google Patents
Dna-integration durch transpositionInfo
- Publication number
- ATE256749T1 ATE256749T1 AT96900546T AT96900546T ATE256749T1 AT E256749 T1 ATE256749 T1 AT E256749T1 AT 96900546 T AT96900546 T AT 96900546T AT 96900546 T AT96900546 T AT 96900546T AT E256749 T1 ATE256749 T1 AT E256749T1
- Authority
- AT
- Austria
- Prior art keywords
- pct
- transposition
- marker
- sec
- interest
- Prior art date
Links
- 230000017105 transposition Effects 0.000 title abstract 2
- 230000010354 integration Effects 0.000 title 1
- 239000003550 marker Substances 0.000 abstract 3
- 108020004414 DNA Proteins 0.000 abstract 2
- 108091028043 Nucleic acid sequence Proteins 0.000 abstract 2
- 108090000623 proteins and genes Proteins 0.000 abstract 2
- 241000192125 Firmicutes Species 0.000 abstract 1
- 239000003242 anti bacterial agent Substances 0.000 abstract 1
- 230000003115 biocidal effect Effects 0.000 abstract 1
- 238000012217 deletion Methods 0.000 abstract 1
- 230000037430 deletion Effects 0.000 abstract 1
- 238000000034 method Methods 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
- C12N15/75—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Bacillus
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
- C12N15/90—Stable introduction of foreign DNA into chromosome
Landscapes
- Genetics & Genomics (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- Organic Chemistry (AREA)
- Biomedical Technology (AREA)
- Chemical & Material Sciences (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Molecular Biology (AREA)
- General Health & Medical Sciences (AREA)
- Plant Pathology (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Mycology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Saccharide Compounds (AREA)
- Enzymes And Modification Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Superconductors And Manufacturing Methods Therefor (AREA)
- Amplifiers (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DK8395 | 1995-01-23 | ||
| DK79995 | 1995-07-06 | ||
| PCT/DK1996/000038 WO1996023073A1 (en) | 1995-01-23 | 1996-01-23 | Dna integration by transposition |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ATE256749T1 true ATE256749T1 (de) | 2004-01-15 |
Family
ID=26063271
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AT96900546T ATE256749T1 (de) | 1995-01-23 | 1996-01-23 | Dna-integration durch transposition |
Country Status (9)
| Country | Link |
|---|---|
| US (1) | US5882888A (de) |
| EP (1) | EP0805867B1 (de) |
| JP (1) | JPH10512450A (de) |
| CN (1) | CN1177380A (de) |
| AT (1) | ATE256749T1 (de) |
| AU (1) | AU4431196A (de) |
| DE (1) | DE69631118T2 (de) |
| DK (1) | DK0805867T3 (de) |
| WO (1) | WO1996023073A1 (de) |
Families Citing this family (80)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6570001B1 (en) * | 1997-06-20 | 2003-05-27 | Institut Pasteur | Polynucleotides and their use for detecting resistance to streptogramin A or to streptogramin B and related compounds |
| US5955310A (en) * | 1998-02-26 | 1999-09-21 | Novo Nordisk Biotech, Inc. | Methods for producing a polypeptide in a bacillus cell |
| WO2000005378A2 (en) | 1998-07-22 | 2000-02-03 | Id-Lelystad Instituut Voor Dierhouderij En Diergezondheid B.V. | Streptococcus suis vaccines and diagnostic tests |
| US6890726B1 (en) | 1999-04-06 | 2005-05-10 | Oklahoma Medical Research Foundation | Method for selecting recombinase variants with altered specificity |
| EP1297170B1 (de) * | 2000-06-23 | 2016-03-02 | Novozymes A/S | Verfahren zur stabilen chromosomalen integration von mehreren kopien von genen |
| ATE400648T1 (de) * | 2000-08-11 | 2008-07-15 | Genencor Int | Transformation von bacillus, transformanten und mutanten-bibliotheken |
| US20020132350A1 (en) * | 2000-09-14 | 2002-09-19 | Pioneer Hi-Bred International, Inc. | Targeted genetic manipulation using Mu bacteriophage cleaved donor complex |
| WO2002061070A2 (en) * | 2001-02-02 | 2002-08-08 | Id-Lelystad, Instituut Voor Dierhouderij En Diergezondheid B.V. | Environmentally regulated genes, involved in the virulence of streptococcus suis |
| ATE399869T1 (de) | 2002-04-10 | 2008-07-15 | Novozymes As | Verbesserte bacillus-wirtszelle |
| US20040224340A1 (en) * | 2003-04-21 | 2004-11-11 | Filutowicz Marcin S. | Displacing a plasmid in a bacterial population |
| WO2004111224A1 (en) | 2003-06-19 | 2004-12-23 | Novozymes A/S | Improved proteases and methods for producing them |
| EP1766002B1 (de) | 2004-06-21 | 2015-11-18 | Novozymes A/S | Mehrere kopien wenigstens zweier offener leserrahmen stabil erhalten in gleicher orientierung |
| DE102005036449A1 (de) * | 2005-08-03 | 2007-02-08 | Robert Bosch Gmbh | Elektrogerät, insbesondere Elektrohandwerkzeug |
| DE602007002568D1 (de) | 2006-05-31 | 2009-11-05 | Novozymes As | Chloramphenicolresistenz-selektion bei bacillus licheniformis |
| DE102007021001A1 (de) | 2007-05-04 | 2008-11-06 | Ab Enzymes Gmbh | Expressionssystem zur antibiotikafreien Produktion von Polypeptiden |
| JP2011067095A (ja) | 2008-01-10 | 2011-04-07 | Ajinomoto Co Inc | 発酵法による目的物質の製造法 |
| WO2010083841A2 (en) * | 2009-01-23 | 2010-07-29 | Nsgene A/S | Improved cell lines and their use in encapsulated cell biodelivery |
| JP2014131487A (ja) | 2011-04-18 | 2014-07-17 | Ajinomoto Co Inc | Ｌ−システインの製造法 |
| EP2758074B1 (de) | 2011-09-23 | 2020-04-22 | Loma Linda University | Bakterienstämme zur expression von methylasegenen und verwendungen davon |
| CN103946372B (zh) | 2011-11-02 | 2016-06-08 | 味之素株式会社 | 用于分泌产生蛋白质的方法 |
| KR20140091742A (ko) | 2011-11-11 | 2014-07-22 | 아지노모토 가부시키가이샤 | 발효법에 의한 목적 물질의 제조법 |
| BR112014028764A2 (pt) | 2012-05-18 | 2017-06-27 | Novozymes As | mutante isolado, métodos para a obtenção do mutante isolado e de um lactobacillus transformante, e, lactobacillus transformante. |
| WO2013178699A1 (en) | 2012-05-31 | 2013-12-05 | Novozymes A/S | Isopropanol production by bacterial hosts |
| BR112015007916B1 (pt) | 2013-05-13 | 2023-04-04 | Ajinomoto Co., Inc | Método para produzir l-aminoácido |
| JP2016165225A (ja) | 2013-07-09 | 2016-09-15 | 味の素株式会社 | 有用物質の製造方法 |
| JP2016192903A (ja) | 2013-09-17 | 2016-11-17 | 味の素株式会社 | 海藻由来バイオマスからのｌ−アミノ酸の製造方法 |
| EP3054005B1 (de) | 2013-10-02 | 2019-11-20 | Ajinomoto Co., Inc. | Heparosanproduzierendes bakterium und heparosanherstellungsverfahren |
| ES2694011T3 (es) | 2013-10-21 | 2018-12-17 | Ajinomoto Co., Inc. | Método para producir L-aminoácido |
| BR112016008830B1 (pt) | 2013-10-23 | 2023-02-23 | Ajinomoto Co., Inc | Método para produzir uma substância alvo |
| JP6582997B2 (ja) | 2014-01-31 | 2019-10-02 | 味の素株式会社 | 変異型グルタミン酸−システインリガーゼ、及び、γ−グルタミルバリルグリシンの製造法 |
| JP2017216881A (ja) | 2014-12-26 | 2017-12-14 | 味の素株式会社 | ジカルボン酸の製造方法 |
| JP6611305B2 (ja) * | 2015-03-31 | 2019-11-27 | 学校法人神奈川大学 | 除去標的ｄｎａの除去方法、ｄｎａカセット、及び発現ベクター |
| WO2016180928A1 (en) | 2015-05-12 | 2016-11-17 | Novozymes A/S | Bacillus licheniformis host cell with deleted lantibiotic gene(s) |
| WO2017073701A2 (en) | 2015-10-27 | 2017-05-04 | Ajinomoto Co., Inc. | Method for producing aldehyde |
| JP6623690B2 (ja) | 2015-10-30 | 2019-12-25 | 味の素株式会社 | グルタミン酸系ｌ−アミノ酸の製造法 |
| CN108463555A (zh) | 2016-01-12 | 2018-08-28 | 味之素株式会社 | 生产苯甲醛的方法 |
| CN110312807B (zh) | 2016-10-21 | 2023-11-17 | 味之素株式会社 | 蛋白质的分泌产生方法 |
| JP7159867B2 (ja) | 2016-10-21 | 2022-10-25 | 味の素株式会社 | タンパク質の分泌生産法 |
| WO2018077796A1 (en) | 2016-10-25 | 2018-05-03 | Novozymes A/S | Flp-mediated genomic integrationin bacillus licheniformis |
| JP2019536449A (ja) | 2016-10-26 | 2019-12-19 | 味の素株式会社 | 目的物質の製造方法 |
| JP7074133B2 (ja) | 2016-10-26 | 2022-05-24 | 味の素株式会社 | 目的物質の製造方法 |
| WO2018079686A1 (en) | 2016-10-26 | 2018-05-03 | Ajinomoto Co., Inc. | Method for producing l-methionine or metabolites requiring s-adenosylmethionine for synthesis |
| WO2018079683A1 (en) | 2016-10-26 | 2018-05-03 | Ajinomoto Co., Inc. | Method for producing objective substance |
| EP3532627A1 (de) | 2016-10-26 | 2019-09-04 | Ajinomoto Co., Inc. | Verfahren zur herstellung einer zielsubstanz |
| JP2019536450A (ja) | 2016-10-27 | 2019-12-19 | 味の素株式会社 | アルデヒドの製造方法 |
| WO2018134386A1 (en) | 2017-01-23 | 2018-07-26 | Novozymes A/S | Host cells and methods for producing double-stranded rna |
| JP7066977B2 (ja) | 2017-04-03 | 2022-05-16 | 味の素株式会社 | Ｌ－アミノ酸の製造法 |
| CN108728477B (zh) * | 2017-04-24 | 2022-02-22 | 华东理工大学 | 一种高效的转座突变系统及构建方法 |
| US10858676B2 (en) | 2017-05-22 | 2020-12-08 | Ajinomoto Co., Inc. | Method for producing objective substance |
| CN112292450A (zh) | 2017-09-25 | 2021-01-29 | 味之素株式会社 | 蛋白质的制造方法和二糖的制造方法 |
| EP3707252A1 (de) | 2017-11-10 | 2020-09-16 | Novozymes A/S | Temperaturempfindliches cas9-protein |
| US11680279B2 (en) | 2017-11-29 | 2023-06-20 | Ajinomoto Co., Inc. | Method for producing objective substance |
| US20210017544A1 (en) | 2017-12-22 | 2021-01-21 | Novozymes A/S | Counter-Selection by Inhibition of Conditionally Essential Genes |
| JP7124338B2 (ja) | 2018-02-27 | 2022-08-24 | 味の素株式会社 | 変異型グルタチオン合成酵素、及び、γ－グルタミルバリルグリシンの製造法 |
| EP3775158A1 (de) | 2018-04-06 | 2021-02-17 | Ajinomoto Co., Inc. | Landwirtschaftliche oder gartenbauliche zusammensetzung mit kulturmedium von bacillus-bakterium |
| EP3783106A4 (de) | 2018-04-20 | 2022-01-05 | Ajinomoto Co., Inc. | Verfahren zur sekretorischen herstellung eines proteins |
| WO2020027251A1 (en) | 2018-08-03 | 2020-02-06 | Ajinomoto Co., Inc. | Method for producing objective substance |
| WO2020085511A1 (ja) | 2018-10-25 | 2020-04-30 | 味の素株式会社 | タンパク質の分泌生産法 |
| CN113366113A (zh) | 2019-01-30 | 2021-09-07 | 诺维信公司 | 同源折叠酶共表达 |
| US20220170003A1 (en) | 2019-03-08 | 2022-06-02 | Novozymes A/S | Means And Methods For Improving Protease Expression |
| BR112021019284A2 (pt) | 2019-03-29 | 2022-02-01 | Ajinomoto Kk | Métodos para produzir alolactose e para produzir um indutor de expressão gênica |
| WO2020226087A1 (ja) | 2019-05-08 | 2020-11-12 | 味の素株式会社 | バニリンの製造方法 |
| US20220275354A1 (en) | 2019-05-15 | 2022-09-01 | Novozymes A/S | TEMPERATURE-SENSITIVE RNA- Guided Endonuclease |
| EP3990629A1 (de) | 2019-06-25 | 2022-05-04 | Novozymes A/S | Gegenauswahl durch hemmung von bedingt essenziellen genen |
| EP4053287A4 (de) | 2019-10-28 | 2023-11-01 | Ajinomoto Co., Inc. | Verfahren zur herstellung von benzaldehyd |
| WO2021177392A1 (ja) | 2020-03-04 | 2021-09-10 | 味の素株式会社 | 変異型トランスグルタミナーゼ |
| WO2021197342A1 (en) * | 2020-03-30 | 2021-10-07 | Institute Of Zoology, Chinese Academy Of Sciences | Active dna transposon systems and methods for use thereof |
| WO2022071061A1 (ja) | 2020-09-29 | 2022-04-07 | 味の素株式会社 | 変異型トランスグルタミナーゼ |
| WO2022092018A1 (ja) | 2020-10-28 | 2022-05-05 | 味の素株式会社 | Ｌ－アミノ酸の製造法 |
| AU2022306337A1 (en) | 2021-07-07 | 2024-02-01 | Ajinomoto Co., Inc. | Method for secretory production of unnatural-amino-acid-containing protein |
| CN117730090A (zh) | 2021-07-13 | 2024-03-19 | 诺维信公司 | 重组角质酶表达 |
| CN118339276A (zh) | 2021-12-10 | 2024-07-12 | 诺维信公司 | 重组细菌中改善的蛋白产量 |
| WO2023200008A1 (ja) | 2022-04-15 | 2023-10-19 | 味の素株式会社 | 培養肉の製造方法 |
| WO2023247514A1 (en) | 2022-06-22 | 2023-12-28 | Novozymes A/S | Recombinant mannanase expression |
| US20240117393A1 (en) | 2022-09-30 | 2024-04-11 | Ajinomoto Co., Inc. | Method for producing l-amino acid |
| JP2026500153A (ja) | 2022-12-05 | 2026-01-06 | ノボザイムス アクティーゼルスカブ | 改変されたｒｎａポリメラーゼ活性 |
| JPWO2024172163A1 (de) | 2023-02-16 | 2024-08-22 | ||
| GB202304540D0 (en) | 2023-03-28 | 2023-05-10 | Univ Tartu | Method of biosynthesis |
| WO2025141838A1 (ja) | 2023-12-28 | 2025-07-03 | 味の素株式会社 | Ｌ－グルタミン酸生産菌およびｌ－グルタミン酸の製造方法 |
| EP4692312A1 (de) | 2023-12-28 | 2026-02-11 | Ajinomoto Co., Inc. | L-glutaminsäure produzierendes bakterium und verfahren zur herstellung von l-glutaminsäure |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| FR2627508B1 (fr) * | 1988-02-22 | 1990-10-05 | Eurolysine | Procede pour l'integration d'un gene choisi sur le chromosome d'une bacterie et bacterie obtenue par ledit procede |
| US5102797A (en) * | 1989-05-26 | 1992-04-07 | Dna Plant Technology Corporation | Introduction of heterologous genes into bacteria using transposon flanked expression cassette and a binary vector system |
| US5527695A (en) * | 1993-01-29 | 1996-06-18 | Purdue Research Foundation | Controlled modification of eukaryotic genomes |
| US5843744A (en) * | 1993-07-08 | 1998-12-01 | Ecogen Inc. | Bacillus thuringiensis Tn5401 proteins |
-
1996
- 1996-01-23 CN CN96192093A patent/CN1177380A/zh active Pending
- 1996-01-23 AT AT96900546T patent/ATE256749T1/de not_active IP Right Cessation
- 1996-01-23 DE DE69631118T patent/DE69631118T2/de not_active Expired - Lifetime
- 1996-01-23 WO PCT/DK1996/000038 patent/WO1996023073A1/en not_active Ceased
- 1996-01-23 AU AU44311/96A patent/AU4431196A/en not_active Abandoned
- 1996-01-23 DK DK96900546T patent/DK0805867T3/da active
- 1996-01-23 US US08/875,154 patent/US5882888A/en not_active Expired - Lifetime
- 1996-01-23 JP JP8522557A patent/JPH10512450A/ja active Pending
- 1996-01-23 EP EP96900546A patent/EP0805867B1/de not_active Expired - Lifetime
Also Published As
| Publication number | Publication date |
|---|---|
| EP0805867A1 (de) | 1997-11-12 |
| EP0805867B1 (de) | 2003-12-17 |
| DE69631118D1 (de) | 2004-01-29 |
| JPH10512450A (ja) | 1998-12-02 |
| WO1996023073A1 (en) | 1996-08-01 |
| AU4431196A (en) | 1996-08-14 |
| US5882888A (en) | 1999-03-16 |
| CN1177380A (zh) | 1998-03-25 |
| DE69631118T2 (de) | 2004-07-01 |
| DK0805867T3 (da) | 2004-04-13 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| RER | Ceased as to paragraph 5 lit. 3 law introducing patent treaties |