ATE272720T1 - Verfahren zum auffinden, nachweisen, anreichern und/oder isolieren von ziel-nucleinsäuresequenzen unter verwendung von von reca ähnlicher rekombinase - Google Patents
Verfahren zum auffinden, nachweisen, anreichern und/oder isolieren von ziel-nucleinsäuresequenzen unter verwendung von von reca ähnlicher rekombinaseInfo
- Publication number
- ATE272720T1 ATE272720T1 AT97937837T AT97937837T ATE272720T1 AT E272720 T1 ATE272720 T1 AT E272720T1 AT 97937837 T AT97937837 T AT 97937837T AT 97937837 T AT97937837 T AT 97937837T AT E272720 T1 ATE272720 T1 AT E272720T1
- Authority
- AT
- Austria
- Prior art keywords
- reca
- recombinase
- detecting
- nucleic acid
- enrichment
- Prior art date
Links
- 238000000034 method Methods 0.000 title abstract 3
- 108010091086 Recombinases Proteins 0.000 title abstract 2
- 102000018120 Recombinases Human genes 0.000 title abstract 2
- 150000007523 nucleic acids Chemical group 0.000 title abstract 2
- 239000000523 sample Substances 0.000 abstract 3
- 102000039446 nucleic acids Human genes 0.000 abstract 1
- 108020004707 nucleic acids Proteins 0.000 abstract 1
- 230000008685 targeting Effects 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6839—Triple helix formation or other higher order conformations in hybridisation assays
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
- C12N15/1093—General methods of preparing gene libraries, not provided for in other subgroups
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6841—In situ hybridisation
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- General Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Physics & Mathematics (AREA)
- General Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Biochemistry (AREA)
- Immunology (AREA)
- Analytical Chemistry (AREA)
- Biomedical Technology (AREA)
- Plant Pathology (AREA)
- Crystallography & Structural Chemistry (AREA)
- Bioinformatics & Computational Biology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Enzymes And Modification Thereof (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP22906196 | 1996-08-29 | ||
| JP34709096 | 1996-12-26 | ||
| PCT/JP1997/003019 WO1998008975A1 (en) | 1996-08-29 | 1997-08-29 | Methods for targeting, enriching, detecting and/or isolating target nucleic acid sequence using reca-like recombinase |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ATE272720T1 true ATE272720T1 (de) | 2004-08-15 |
Family
ID=26528611
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AT97937837T ATE272720T1 (de) | 1996-08-29 | 1997-08-29 | Verfahren zum auffinden, nachweisen, anreichern und/oder isolieren von ziel-nucleinsäuresequenzen unter verwendung von von reca ähnlicher rekombinase |
Country Status (7)
| Country | Link |
|---|---|
| US (1) | US6335164B1 (de) |
| EP (1) | EP0939834B1 (de) |
| AT (1) | ATE272720T1 (de) |
| AU (1) | AU712873B2 (de) |
| CA (1) | CA2263815A1 (de) |
| DE (1) | DE69730145T2 (de) |
| WO (1) | WO1998008975A1 (de) |
Families Citing this family (27)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP3065035B2 (ja) * | 1998-10-01 | 2000-07-12 | ダイキン工業株式会社 | 高性能なＲｅｃＡ様組換え酵素／１本鎖核酸プローブ複合体の調製方法及びその利用 |
| JP4509307B2 (ja) * | 2000-06-07 | 2010-07-21 | 株式会社アイシン・コスモス研究所 | ＲｅｃＡタンパク質を用いてライブラリーを加工する方法 |
| JP4616988B2 (ja) * | 2000-12-20 | 2011-01-19 | 株式会社アイシン・コスモス研究所 | 三本鎖ｄｎａ形成技術を応用したｄｎａ多型検出方法 |
| EP1414840A4 (de) * | 2001-03-27 | 2005-04-13 | Univ Delaware | Genomics-anwendungen für modifizierte oligonukleotide |
| JP5128031B2 (ja) * | 2001-03-28 | 2013-01-23 | 株式会社アイシン・コスモス研究所 | ＲｅｃＡタンパク質を用いて標識が導入された核酸を製造する方法 |
| CA2444649C (en) | 2001-04-20 | 2012-10-02 | The Penn State Research Foundation | Methods for nucleic acid manipulation |
| US7468244B2 (en) * | 2001-09-27 | 2008-12-23 | University Of Delaware | Polymorphism detection and separation |
| AU2002341898A2 (en) * | 2001-09-28 | 2003-04-07 | University Of Delaware | Polymorphism detection and separation |
| KR100468624B1 (ko) * | 2002-01-03 | 2005-01-27 | 한국생명공학연구원 | 미생물 리컴비네이즈를 이용한 색소체 형질전환 방법 |
| US7399590B2 (en) | 2002-02-21 | 2008-07-15 | Asm Scientific, Inc. | Recombinase polymerase amplification |
| US8030000B2 (en) | 2002-02-21 | 2011-10-04 | Alere San Diego, Inc. | Recombinase polymerase amplification |
| US7270981B2 (en) | 2002-02-21 | 2007-09-18 | Asm Scientific, Inc. | Recombinase polymerase amplification |
| US7759057B2 (en) * | 2004-02-04 | 2010-07-20 | Ut-Battelle, Llc | Method for analyzing microbial communities |
| AU2005218507A1 (en) * | 2004-02-28 | 2005-09-15 | Chang-Ning J. Wang | Nucleic acid complexes |
| WO2007096702A2 (en) | 2005-07-25 | 2007-08-30 | Asm Scientific, Inc. | Methods for multiplexing recombinase polymerase amplification |
| US8071308B2 (en) | 2006-05-04 | 2011-12-06 | Alere San Diego, Inc. | Recombinase polymerase amplification |
| US9689031B2 (en) | 2007-07-14 | 2017-06-27 | Ionian Technologies, Inc. | Nicking and extension amplification reaction for the exponential amplification of nucleic acids |
| JP2010017178A (ja) * | 2008-06-11 | 2010-01-28 | Sumitomo Chemical Co Ltd | Ｄｎａを定量又は検出する方法 |
| US20120156728A1 (en) | 2010-12-17 | 2012-06-21 | Life Technologies Corporation | Clonal amplification of nucleic acid on solid surface with template walking |
| US9309566B2 (en) | 2010-12-17 | 2016-04-12 | Life Technologies Corporation | Methods, compositions, systems, apparatuses and kits for nucleic acid amplification |
| US9309557B2 (en) | 2010-12-17 | 2016-04-12 | Life Technologies Corporation | Nucleic acid amplification |
| US9334531B2 (en) | 2010-12-17 | 2016-05-10 | Life Technologies Corporation | Nucleic acid amplification |
| JP5847076B2 (ja) | 2009-05-20 | 2016-01-20 | バイオサイト インコーポレイテッド | Ｄｎａグリコシラーゼ／リアーゼおよびａｐエンドヌクレアーゼ基質 |
| EP2438196B1 (de) | 2009-06-05 | 2016-12-21 | Alere San Diego, Inc. | Kits und reagenzien für rekombinase-polymerase-amplifikationen |
| EP2694666B1 (de) | 2011-04-07 | 2018-05-23 | Alere San Diego, Inc. | Überwachung von rekombinase-polymerase-verstärkungsmischungen |
| WO2014020137A1 (en) * | 2012-08-02 | 2014-02-06 | Qiagen Gmbh | Recombinase mediated targeted dna enrichment for next generation sequencing |
| US20180282786A1 (en) * | 2017-04-01 | 2018-10-04 | Michael Joseph Pugia | Methods and apparatus for selective nucleic acid separation |
Family Cites Families (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4888274A (en) | 1985-09-18 | 1989-12-19 | Yale University | RecA nucleoprotein filament and methods |
| US5223414A (en) | 1990-05-07 | 1993-06-29 | Sri International | Process for nucleic acid hybridization and amplification |
| ATE153707T1 (de) * | 1990-05-07 | 1997-06-15 | Daikin Ind Ltd | Diagnostische anwendungen der bildung von doppelten d-loops. |
| US5445090A (en) | 1991-07-25 | 1995-08-29 | Mim Industries, Inc. | Interchangeable clamp for use in a sewing machine |
| US5506098A (en) | 1991-09-04 | 1996-04-09 | Daikin Industries, Ltd. | In situ hybridization method |
| AU4115693A (en) | 1992-04-24 | 1993-11-29 | Sri International | In vivo homologous sequence targeting in eukaryotic cells |
| US5468629A (en) * | 1993-04-13 | 1995-11-21 | Calhoun; Cornelia | Method of promoting in vitro homologous recombination transfection in mammalian cells using the RecA protein |
| US5965361A (en) * | 1993-12-28 | 1999-10-12 | Daikin Industries, Ltd. | In-situ hybridization method using RecA protein and RecA protein having marker or ligand for use in said method |
-
1997
- 1997-08-29 CA CA002263815A patent/CA2263815A1/en not_active Abandoned
- 1997-08-29 AT AT97937837T patent/ATE272720T1/de not_active IP Right Cessation
- 1997-08-29 US US09/147,751 patent/US6335164B1/en not_active Expired - Fee Related
- 1997-08-29 DE DE69730145T patent/DE69730145T2/de not_active Expired - Fee Related
- 1997-08-29 EP EP97937837A patent/EP0939834B1/de not_active Expired - Lifetime
- 1997-08-29 WO PCT/JP1997/003019 patent/WO1998008975A1/en not_active Ceased
- 1997-08-29 AU AU40320/97A patent/AU712873B2/en not_active Ceased
Also Published As
| Publication number | Publication date |
|---|---|
| EP0939834A1 (de) | 1999-09-08 |
| DE69730145D1 (de) | 2004-09-09 |
| AU4032097A (en) | 1998-03-19 |
| DE69730145T2 (de) | 2005-07-14 |
| CA2263815A1 (en) | 1998-03-05 |
| EP0939834B1 (de) | 2004-08-04 |
| WO1998008975A1 (en) | 1998-03-05 |
| US6335164B1 (en) | 2002-01-01 |
| AU712873B2 (en) | 1999-11-18 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| RER | Ceased as to paragraph 5 lit. 3 law introducing patent treaties |