ATE274698T1 - Verfahren zur kovalenten immobilisierung von biomolekülen an einem träger mittels eines his- tags - Google Patents

Verfahren zur kovalenten immobilisierung von biomolekülen an einem träger mittels eines his- tags

Info

Publication number
ATE274698T1
ATE274698T1 AT98938647T AT98938647T ATE274698T1 AT E274698 T1 ATE274698 T1 AT E274698T1 AT 98938647 T AT98938647 T AT 98938647T AT 98938647 T AT98938647 T AT 98938647T AT E274698 T1 ATE274698 T1 AT E274698T1
Authority
AT
Austria
Prior art keywords
tag
biomolecules
present
membrane
carrier
Prior art date
Application number
AT98938647T
Other languages
English (en)
Inventor
Alfons Bosman
Wijnendaele Frans Van
Den Broeck Dirk Van
De Voorde Andre Van
Original Assignee
Innogenetics Nv
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Innogenetics Nv filed Critical Innogenetics Nv
Application granted granted Critical
Publication of ATE274698T1 publication Critical patent/ATE274698T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54353—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals with ligand attached to the carrier via a chemical coupling agent
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54393—Improving reaction conditions or stability, e.g. by coating or irradiation of surface, by reduction of non-specific binding, by promotion of specific binding

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Zoology (AREA)
  • Cell Biology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Pathology (AREA)
  • Analytical Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • General Engineering & Computer Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Peptides Or Proteins (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Investigating Or Analyzing Non-Biological Materials By The Use Of Chemical Means (AREA)
AT98938647T 1997-06-25 1998-06-25 Verfahren zur kovalenten immobilisierung von biomolekülen an einem träger mittels eines his- tags ATE274698T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP97870095 1997-06-25
PCT/EP1998/003883 WO1999000670A1 (en) 1997-06-25 1998-06-25 Methods for covalent immobilisation of biomolecules to a carrier by means of a his-tag

Publications (1)

Publication Number Publication Date
ATE274698T1 true ATE274698T1 (de) 2004-09-15

Family

ID=8231014

Family Applications (1)

Application Number Title Priority Date Filing Date
AT98938647T ATE274698T1 (de) 1997-06-25 1998-06-25 Verfahren zur kovalenten immobilisierung von biomolekülen an einem träger mittels eines his- tags

Country Status (7)

Country Link
EP (1) EP0991944B1 (de)
AT (1) ATE274698T1 (de)
AU (1) AU746325B2 (de)
CA (1) CA2293777A1 (de)
DE (1) DE69825896T2 (de)
ES (1) ES2227868T3 (de)
WO (1) WO1999000670A1 (de)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6593308B2 (en) * 1999-12-03 2003-07-15 The Regents Of The University Of California Targeted drug delivery with a hyaluronan ligand
GB0424560D0 (en) 2004-11-08 2004-12-08 Leuven K U Res & Dev Heart valve tissue engineering
ES2597849T3 (es) * 2007-10-09 2017-01-23 Polytherics Limited Proteínas y péptidos conjugados novedosos
GB0804495D0 (en) * 2008-03-11 2008-04-16 Iti Scotland Ltd Surface attachment
JP5476376B2 (ja) * 2008-07-16 2014-04-23 ラジオメーター・メディカル・アー・ペー・エス 高性能固相
DE102010023452B4 (de) * 2010-06-11 2012-11-08 Bruker Daltonik Gmbh Massenspektrometrische Messung von β-Lactamase-Resistenzen
CN105483111A (zh) * 2014-09-26 2016-04-13 丰益(上海)生物技术研发中心有限公司 固定化脂酶及其制备方法
EP3999025B1 (de) * 2019-07-19 2025-02-26 The Regents Of The University Of Michigan Poröse selbstheilende polymermatrix zur verkapselung von aktiven makromolekülen und verfahren

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5674677A (en) * 1994-12-21 1997-10-07 The Center For Innovative Technology Immunoassay technique using histidine tags, metals, and chelating agents

Also Published As

Publication number Publication date
AU8729098A (en) 1999-01-19
DE69825896D1 (de) 2004-09-30
WO1999000670A1 (en) 1999-01-07
EP0991944A1 (de) 2000-04-12
EP0991944B1 (de) 2004-08-25
ES2227868T3 (es) 2005-04-01
AU746325B2 (en) 2002-04-18
WO1999000670A8 (en) 2000-03-02
CA2293777A1 (en) 1999-01-07
DE69825896T2 (de) 2005-09-15

Similar Documents

Publication Publication Date Title
US5079155A (en) Fluorocarbon polymer support for chromatographic separations, diagnostic assays and enzyme immobilization
ATE191237T1 (de) Verfahren zur immobilisierung von nukleinsäuresonden an polystyroloberflächen
ATE42317T1 (de) Voraktivierte kunststoffoberflaechen zur immobilisierung von organisch-chemischen und biologischen materialien, verfahren zur herstellung und verwendung derselben.
DE60040794D1 (de) Verfahren zur Immobilisierung von Oligonukleotiden auf festem Trägermaterial
ATE274698T1 (de) Verfahren zur kovalenten immobilisierung von biomolekülen an einem träger mittels eines his- tags
US20100234240A1 (en) Surface modification
US4268419A (en) Support matrices for immobilized enzymes
US5494815A (en) Immobilization of biochemical substances on a carrier containing a layer of an olefinic-unsaturated, epoxyfunctional polysiloxane
US5532170A (en) Processing analytical reagents
US4292199A (en) Method of preparing a support matrix
US4549011A (en) Modified sheet of material and method of making and using same in connection with biochemical procedures
Kobos et al. A novel fluorocarbon-based immobilization technology
DE59909404D1 (de) Verfahren zum nachweis von antikörpern oder antigenen sowie zur blutgruppenbestimmung
Wang et al. Comparison of covalent immobilization of amylase on polystyrene pellets with pentaethylenehexamine and pentaethylene glycol spacers
US20200340983A1 (en) Method for immobilizing ligand having amino group
AU597568B2 (en) Method for producing high-active biologically efficient compounds immobilized on a carrier
PL102119B1 (pl) A process of producing the base for immobilizing enzymes
US3930950A (en) Method of immobilizing an enzyme
Godjevargova et al. Influence of matrix on external mass transfer resistance in immobilized urease membranes
JP6781154B2 (ja) リガンドの固定化方法
DE3863544D1 (de) Immunoassayverfahren zur blutgruppenbestimmung.
IL171177A (en) Method for the preparation of photoreactive polymers with molecules immobilized thereon
EP0267521A3 (de) Verfahren zum Nachweis von Nukleinsäure-Hybriden
US20060099642A1 (en) Solid-phase substrate for immobilizing biomolecules
JPH05261281A (ja) 生理活性物質固定化担体とその製法

Legal Events

Date Code Title Description
UEP Publication of translation of european patent specification

Ref document number: 0991944

Country of ref document: EP

REN Ceased due to non-payment of the annual fee