ATE277175T1 - Ein verfahren für die plasmidreinigung in grossem massstab - Google Patents

Ein verfahren für die plasmidreinigung in grossem massstab

Info

Publication number
ATE277175T1
ATE277175T1 AT95927143T AT95927143T ATE277175T1 AT E277175 T1 ATE277175 T1 AT E277175T1 AT 95927143 T AT95927143 T AT 95927143T AT 95927143 T AT95927143 T AT 95927143T AT E277175 T1 ATE277175 T1 AT E277175T1
Authority
AT
Austria
Prior art keywords
large scale
cleaning
disclosed
plasmid dna
plasmids
Prior art date
Application number
AT95927143T
Other languages
English (en)
Inventor
Ann L Lee
Sangeetha Sagar
Original Assignee
Merck & Co Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Merck & Co Inc filed Critical Merck & Co Inc
Application granted granted Critical
Publication of ATE277175T1 publication Critical patent/ATE277175T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/06—Lysis of microorganisms
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1017—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by filtration, e.g. using filters, frits, membranes

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Mycology (AREA)
  • Virology (AREA)
  • Medicinal Chemistry (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Saccharide Compounds (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Detergent Compositions (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
AT95927143T 1994-07-15 1995-07-11 Ein verfahren für die plasmidreinigung in grossem massstab ATE277175T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US27557194A 1994-07-15 1994-07-15
PCT/US1995/008749 WO1996002658A1 (en) 1994-07-15 1995-07-11 A method for large scale plasmid purification

Publications (1)

Publication Number Publication Date
ATE277175T1 true ATE277175T1 (de) 2004-10-15

Family

ID=23052893

Family Applications (1)

Application Number Title Priority Date Filing Date
AT95927143T ATE277175T1 (de) 1994-07-15 1995-07-11 Ein verfahren für die plasmidreinigung in grossem massstab

Country Status (8)

Country Link
EP (1) EP0771355B1 (de)
JP (1) JPH10503086A (de)
AT (1) ATE277175T1 (de)
AU (1) AU708798B2 (de)
CA (1) CA2192342A1 (de)
DE (1) DE69533552T2 (de)
ES (1) ES2227557T3 (de)
WO (1) WO1996002658A1 (de)

Families Citing this family (22)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AR003122A1 (es) * 1995-05-19 1998-07-08 Merck & Co Inc Un proceso para aislacion y purificacion de plasmidos en fermentadores de gran escala y el adn aislado y purificado obtenido mediante dicho proceso.
ATE238321T1 (de) 1996-02-06 2003-05-15 Roche Diagnostics Gmbh Verfahren zur herstellung von gereinigter nukleinsäure und deren verwendung
GB9602825D0 (en) * 1996-02-12 1996-04-10 Therexsys Ltd Method of plasmid dna production and purification
US5981735A (en) 1996-02-12 1999-11-09 Cobra Therapeutics Limited Method of plasmid DNA production and purification
US7026468B2 (en) 1996-07-19 2006-04-11 Valentis, Inc. Process and equipment for plasmid purification
US7807822B2 (en) * 1996-08-01 2010-10-05 Robert Bridenbaugh Methods for purifying nucleic acids
EP0853123A1 (de) * 1997-01-10 1998-07-15 Roche Diagnostics GmbH Reinigung von DNA durch Cross-Flow-Filtration
FR2773818B1 (fr) * 1998-01-21 2000-02-18 Pasteur Merieux Serums Vacc Procede et dispositif de lyse de bacteries
DE69922740T2 (de) 1998-05-11 2005-12-08 Tosoh Corp., Shinnanyo Methode zur Trennung von Nucleinsäuren mittels Flüssigchromatographie
US6268492B1 (en) 1998-08-10 2001-07-31 Chiron Corporation Methods for purifying non-chromosomal nucleic acid molecules from cells
US6664049B1 (en) 1999-01-20 2003-12-16 Aventis Pasteur S.A. Method and device for cell lysis
PL364816A1 (en) 1999-07-23 2004-12-13 Genentech, Inc. Method for rnase- and organic solvent-free plasmid dna purification using tangential flow filtration
US20020012990A1 (en) 1999-12-22 2002-01-31 Lander Russel Jackson Process for the scaleable purification of plasmid DNA
US6406892B1 (en) 2001-05-23 2002-06-18 Bio-Rad Laboratories, Inc. Acetate-free purification of plasmid DNA on hydroxyapatite
EP1321176A1 (de) * 2001-12-18 2003-06-25 Boehringer Ingelheim International GmbH Verfahren und Vorrichtung zur Isolierung und Reinigung eines bestimmten Polynukleotides in industriellem Umfang
ATE420167T1 (de) * 2002-12-23 2009-01-15 Vical Inc Reinigungsverfahren für plasmid-dna
US8501402B2 (en) 2003-03-24 2013-08-06 Boehringer Ingelheim Rcv Gmbh & Co Kg Methods and devices for producing biomolecules
EP2246413A3 (de) 2004-04-19 2011-10-26 Centelion Verfahren zur Herstellung einer Plasmid-DNA in pharmazeutischer Qualität
US7527960B2 (en) 2004-06-25 2009-05-05 Merial Limited Avipox recombinants expressing foot and mouth disease virus genes
CN101310022B (zh) 2005-01-31 2013-01-16 默沙东公司 大规模生产质粒dna的上游和下游纯化方法
EP2088196A1 (de) 2008-02-08 2009-08-12 Boehringer Ingelheim RCV GmbH & Co KG Verfahren und Vorrichtungen zur Herstellung von Biomolekülen
CN120882735A (zh) 2023-01-25 2025-10-31 诺韦尔生物技术美国公司 用于产品制造的表达系统

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3928642A (en) * 1975-01-22 1975-12-23 Lilly Co Eli Process for lysing mycelial waste
US4830969A (en) * 1981-08-31 1989-05-16 The Research Foundation Of State University Of New York Process for the rapid and simple isolation of nucleic acids
US5256549A (en) * 1986-03-28 1993-10-26 Chiron Corporation Purification of synthetic oligomers
DE69031305T2 (de) * 1989-11-03 1998-03-26 Univ Vanderbilt Verfahren zur in vivo-verabreichung von funktionsfähigen fremden genen
WO1993025673A1 (en) * 1992-06-04 1993-12-23 The Regents Of The University Of California In vivo gene therapy with intron-free sequence of interest
US5834441A (en) * 1993-09-13 1998-11-10 Rhone-Poulenc Rorer Pharmaceuticals Inc. Adeno-associated viral (AAV) liposomes and methods related thereto
AR003122A1 (es) * 1995-05-19 1998-07-08 Merck & Co Inc Un proceso para aislacion y purificacion de plasmidos en fermentadores de gran escala y el adn aislado y purificado obtenido mediante dicho proceso.

Also Published As

Publication number Publication date
CA2192342A1 (en) 1996-02-01
JPH10503086A (ja) 1998-03-24
DE69533552T2 (de) 2005-09-22
EP0771355A1 (de) 1997-05-07
EP0771355B1 (de) 2004-09-22
AU708798B2 (en) 1999-08-12
AU3126295A (en) 1996-02-16
DE69533552D1 (de) 2004-10-28
EP0771355A4 (de) 1997-08-20
WO1996002658A1 (en) 1996-02-01
ES2227557T3 (es) 2005-04-01

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