ATE278773T1 - Verfahren zur expression und isolierung von rekombinanten proteinen und rekombinanten proteinprodukten von pflanzen, pflanzlichen geweben oder kultivierten pflanzenzellen - Google Patents

Verfahren zur expression und isolierung von rekombinanten proteinen und rekombinanten proteinprodukten von pflanzen, pflanzlichen geweben oder kultivierten pflanzenzellen

Info

Publication number
ATE278773T1
ATE278773T1 AT00931527T AT00931527T ATE278773T1 AT E278773 T1 ATE278773 T1 AT E278773T1 AT 00931527 T AT00931527 T AT 00931527T AT 00931527 T AT00931527 T AT 00931527T AT E278773 T1 ATE278773 T1 AT E278773T1
Authority
AT
Austria
Prior art keywords
plant
cells
expressing
fusion protein
recombinant protein
Prior art date
Application number
AT00931527T
Other languages
English (en)
Inventor
Ziv Shani
Oded Shoseyov
Original Assignee
Cbd Technologies Ltd
Yissum Res Dev Co
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Cbd Technologies Ltd, Yissum Res Dev Co filed Critical Cbd Technologies Ltd
Application granted granted Critical
Publication of ATE278773T1 publication Critical patent/ATE278773T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/315—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Streptococcus (G), e.g. Enterococci
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8257—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits for the production of primary gene products, e.g. pharmaceutical products, interferon
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/035—Fusion polypeptide containing a localisation/targetting motif containing a signal for targeting to the external surface of a cell, e.g. to the outer membrane of Gram negative bacteria, GPI- anchored eukaryote proteins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/04—Fusion polypeptide containing a localisation/targetting motif containing an ER retention signal such as a C-terminal HDEL motif
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/50—Fusion polypeptide containing protease site
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/70—Fusion polypeptide containing domain for protein-protein interaction
    • C07K2319/705—Fusion polypeptide containing domain for protein-protein interaction containing a protein-A fusion

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Plant Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Cell Biology (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
AT00931527T 1999-06-10 2000-06-07 Verfahren zur expression und isolierung von rekombinanten proteinen und rekombinanten proteinprodukten von pflanzen, pflanzlichen geweben oder kultivierten pflanzenzellen ATE278773T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US09/329,234 US6331416B1 (en) 1999-06-10 1999-06-10 Process of expressing and isolating recombinant proteins and recombinant protein products from plants, plant derived tissues or cultured plant cells
PCT/IL2000/000330 WO2000077174A1 (en) 1999-06-10 2000-06-07 Process of expressing and isolating recombinant proteins and recombinant protein products from plants, plant derived tissues or cultured plant cells

Publications (1)

Publication Number Publication Date
ATE278773T1 true ATE278773T1 (de) 2004-10-15

Family

ID=23284473

Family Applications (1)

Application Number Title Priority Date Filing Date
AT00931527T ATE278773T1 (de) 1999-06-10 2000-06-07 Verfahren zur expression und isolierung von rekombinanten proteinen und rekombinanten proteinprodukten von pflanzen, pflanzlichen geweben oder kultivierten pflanzenzellen

Country Status (8)

Country Link
US (1) US6331416B1 (de)
EP (1) EP1185624B1 (de)
JP (1) JP2003502032A (de)
AT (1) ATE278773T1 (de)
AU (2) AU5136900A (de)
CA (1) CA2376392A1 (de)
DE (1) DE60014635D1 (de)
WO (2) WO2000077175A1 (de)

Families Citing this family (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE10049587A1 (de) * 2000-10-06 2002-05-02 Icon Genetics Ag Vektorsystem für Pflanzen
DE10061150A1 (de) 2000-12-08 2002-06-13 Icon Genetics Ag Verfahren und Vektoren zur Erzeugung von transgenen Pflanzen
DE10102389A1 (de) 2001-01-19 2002-08-01 Icon Genetics Ag Verfahren und Vektoren zur Plastidentransformation höherer Pflanzen
DE10114209A1 (de) * 2001-03-23 2002-12-05 Icon Genetics Ag Ortsgerichtete Transformation durch Verwendung von Amplifikationsvektoren
DE10115507A1 (de) * 2001-03-29 2002-10-10 Icon Genetics Ag Verfahren zur Kodierung von Information in Nukleinsäuren eines genetisch veränderten Organismus
DE10121283B4 (de) 2001-04-30 2011-08-11 Icon Genetics GmbH, 80333 Verfahren und Vektoren zur Amplifikation oder Expression von gewünschten Nucleinsäuresequenzen in Pflanzen
DE10132780A1 (de) * 2001-07-06 2003-01-16 Icon Genetics Ag Plastidäre Genexpression über autonom replizierende Vektoren
DE10143238A1 (de) * 2001-09-04 2003-03-20 Icon Genetics Ag Identifizierung eukaryotischer interner Ribosomen-Eingangsstellen (IRES)-Elemente
DE10143237A1 (de) * 2001-09-04 2003-03-20 Icon Genetics Ag Herstellung künstlicher interner ribosomaler Eingangsstellenelemente (Ires-Elemente)
CN101993907B (zh) * 2002-01-08 2018-12-28 迈克尔·R·拉尔布 表达civps或内含肽修饰蛋白的转基因植物及其制备方法
US20040003432A1 (en) * 2002-05-06 2004-01-01 Pharmacia Corporation Production of hexosamines and uses thereof
JP2007503212A (ja) * 2003-08-27 2007-02-22 オーアールエフ・リフタエクニ・エイチエフ 内在性貯蔵タンパク質の標的化抑制を介した、植物種子中で異種ポリペプチドの蓄積を亢進させる方法
JP2007503211A (ja) * 2003-08-27 2007-02-22 オーアールエフ・リフタエクニ・エイチエフ 植物から組換えタンパク質を精製するための非変性プロセス
JP2007517504A (ja) * 2003-12-09 2007-07-05 ベントリア バイオサイエンス 融合担体として種子貯蔵蛋白を利用する植物種子中の融合ポリペプチドの高水準発現
EP1672075A1 (de) * 2004-12-17 2006-06-21 Bayer CropScience GmbH Transformierte Pflanzen, die Dextransucrase exprimieren und eine veränderte Stärke synthetisieren
WO2007081772A2 (en) 2006-01-04 2007-07-19 Exelixis Plant Sciences, Inc. Taxus transformation, transformed cells, and related compositions and methods
FR2920429B1 (fr) * 2007-08-30 2012-10-05 Lfb Biotechnologies Procede de purification du facteur viii et du facteur von willebrand
WO2016079739A2 (en) * 2014-11-20 2016-05-26 Yissum Research Development Company Of The Hebrew University Of Jerusalem Ltd. Compositions and methods for producing polypeptides with a modified glycosylation pattern in plant cells
CN110831432A (zh) * 2017-01-24 2020-02-21 旗舰创业创新五公司 用于农业的组合物和相关方法

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5474925A (en) * 1991-12-19 1995-12-12 Agracetus, Inc. Immobilized proteins in cotton fiber
US5496934A (en) * 1993-04-14 1996-03-05 Yissum Research Development Company Of The Hebrew University Of Jerusalem Nucleic acids encoding a cellulose binding domain
KR20000036077A (ko) * 1996-09-12 2000-06-26 한스 루돌프 하우스, 헨리테 브룬너, 베아트리체 귄터 셀룰로스 분해 효소를 발현하는 트랜스젠 식물
US5981835A (en) * 1996-10-17 1999-11-09 Wisconsin Alumni Research Foundation Transgenic plants as an alternative source of lignocellulosic-degrading enzymes

Also Published As

Publication number Publication date
DE60014635D1 (de) 2004-11-11
CA2376392A1 (en) 2000-12-21
EP1185624B1 (de) 2004-10-06
EP1185624A1 (de) 2002-03-13
AU5136900A (en) 2001-01-02
US6331416B1 (en) 2001-12-18
WO2000077175A1 (en) 2000-12-21
WO2000077174A1 (en) 2000-12-21
AU4947500A (en) 2001-01-02
JP2003502032A (ja) 2003-01-21
EP1185624A4 (de) 2003-06-04

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