ATE279912T1 - Verfahren zur zelltransfektion mit liposomal- verkapselten nukleinsäuren - Google Patents

Verfahren zur zelltransfektion mit liposomal- verkapselten nukleinsäuren

Info

Publication number
ATE279912T1
ATE279912T1 AT96917085T AT96917085T ATE279912T1 AT E279912 T1 ATE279912 T1 AT E279912T1 AT 96917085 T AT96917085 T AT 96917085T AT 96917085 T AT96917085 T AT 96917085T AT E279912 T1 ATE279912 T1 AT E279912T1
Authority
AT
Austria
Prior art keywords
nucleic acids
encapsuled
liposomal
cell transfection
nucleic acid
Prior art date
Application number
AT96917085T
Other languages
English (en)
Inventor
Alain Thierry
Anatoly Dritschilo
Original Assignee
Univ Georgetown
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Georgetown filed Critical Univ Georgetown
Application granted granted Critical
Publication of ATE279912T1 publication Critical patent/ATE279912T1/de

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/88—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation using microencapsulation, e.g. using amphiphile liposome vesicle
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K9/00—Medicinal preparations characterised by special physical form
    • A61K9/10—Dispersions; Emulsions
    • A61K9/127—Synthetic bilayered vehicles, e.g. liposomes or liposomes with cholesterol as the only non-phosphatidyl surfactant
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K9/00—Medicinal preparations characterised by special physical form
    • A61K9/10—Dispersions; Emulsions
    • A61K9/127—Synthetic bilayered vehicles, e.g. liposomes or liposomes with cholesterol as the only non-phosphatidyl surfactant
    • A61K9/1277—Preparation processes; Proliposomes

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Dispersion Chemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Veterinary Medicine (AREA)
  • Public Health (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Animal Behavior & Ethology (AREA)
  • Wood Science & Technology (AREA)
  • Epidemiology (AREA)
  • Biomedical Technology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Medicinal Chemistry (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Preparation (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
AT96917085T 1995-06-07 1996-06-06 Verfahren zur zelltransfektion mit liposomal- verkapselten nukleinsäuren ATE279912T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US08/483,090 US5756122A (en) 1995-06-07 1995-06-07 Liposomally encapsulated nucleic acids having high entrapment efficiencies, method of manufacturer and use thereof for transfection of targeted cells
PCT/US1996/008619 WO1996040062A1 (en) 1995-06-07 1996-06-06 A method of transfection of cells using liposomally encapsulated nucleic acids

Publications (1)

Publication Number Publication Date
ATE279912T1 true ATE279912T1 (de) 2004-11-15

Family

ID=23918616

Family Applications (1)

Application Number Title Priority Date Filing Date
AT96917085T ATE279912T1 (de) 1995-06-07 1996-06-06 Verfahren zur zelltransfektion mit liposomal- verkapselten nukleinsäuren

Country Status (7)

Country Link
US (1) US5756122A (de)
EP (1) EP0835099B1 (de)
AT (1) ATE279912T1 (de)
AU (1) AU700376B2 (de)
CA (1) CA2223637A1 (de)
DE (1) DE69633669T2 (de)
WO (1) WO1996040062A1 (de)

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EP0835099A1 (de) 1998-04-15
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EP0835099A4 (de) 2000-11-08
CA2223637A1 (en) 1996-12-19
DE69633669D1 (de) 2004-11-25
AU5976896A (en) 1996-12-30
AU700376B2 (en) 1999-01-07
WO1996040062A1 (en) 1996-12-19
US5756122A (en) 1998-05-26

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