ATE285479T1 - Template abhängige ligierung mit pna-dna chimerischen sonden - Google Patents

Template abhängige ligierung mit pna-dna chimerischen sonden

Info

Publication number
ATE285479T1
ATE285479T1 AT00968853T AT00968853T ATE285479T1 AT E285479 T1 ATE285479 T1 AT E285479T1 AT 00968853 T AT00968853 T AT 00968853T AT 00968853 T AT00968853 T AT 00968853T AT E285479 T1 ATE285479 T1 AT E285479T1
Authority
AT
Austria
Prior art keywords
ligation
pna
dna chimeric
template dependent
oligonucleotide
Prior art date
Application number
AT00968853T
Other languages
English (en)
Inventor
Michael Egholm
Caifu Chen
Original Assignee
Applera Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Applera Corp filed Critical Applera Corp
Application granted granted Critical
Publication of ATE285479T1 publication Critical patent/ATE285479T1/de

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
    • C12Q1/6862Ligase chain reaction [LCR]

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
AT00968853T 1999-10-08 2000-10-06 Template abhängige ligierung mit pna-dna chimerischen sonden ATE285479T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US09/416,003 US6297016B1 (en) 1999-10-08 1999-10-08 Template-dependent ligation with PNA-DNA chimeric probes
PCT/US2000/027730 WO2001027326A2 (en) 1999-10-08 2000-10-06 Template-dependent ligation with pna-dna chimeric probes

Publications (1)

Publication Number Publication Date
ATE285479T1 true ATE285479T1 (de) 2005-01-15

Family

ID=23648121

Family Applications (1)

Application Number Title Priority Date Filing Date
AT00968853T ATE285479T1 (de) 1999-10-08 2000-10-06 Template abhängige ligierung mit pna-dna chimerischen sonden

Country Status (8)

Country Link
US (2) US6297016B1 (de)
EP (1) EP1220953B1 (de)
JP (2) JP3789817B2 (de)
AT (1) ATE285479T1 (de)
AU (1) AU768240B2 (de)
CA (1) CA2353801A1 (de)
DE (1) DE60016947T2 (de)
WO (1) WO2001027326A2 (de)

Families Citing this family (98)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6368801B1 (en) * 2000-04-12 2002-04-09 Molecular Staging, Inc. Detection and amplification of RNA using target-mediated ligation of DNA by RNA ligase
JP3596868B2 (ja) * 2000-11-24 2004-12-02 キヤノン株式会社 末端標識プローブアレイのプローブの量の評価方法、及び、該標識プローブアレイを用いた標的物質量の評価方法
WO2002048404A2 (en) * 2000-12-14 2002-06-20 Gen-Probe Incorporated Method and kit for enhancing the association rates of polynucleotides
US7256275B2 (en) * 2001-03-09 2007-08-14 Boston Probes, Inc. Methods, kits and compositions pertaining to combination oligomers and libraries for their preparation
JP2004521630A (ja) * 2001-03-15 2004-07-22 エグザクト サイエンシーズ コーポレイション 変更検出のための方法
ATE509272T1 (de) * 2001-11-09 2011-05-15 3Dbiosurfaces Technologies Llc Substrate mit hochliegendem oberflächenbereich für mikroarrays sowie verfahren zur herstellung davon
US6686162B2 (en) 2001-12-04 2004-02-03 Quest Diagnostics Investments, Incorporated Oligonucleotides and methods for detecting Borrelia burgdorferi
US6946245B2 (en) 2001-12-04 2005-09-20 Quest Diagnostics Investments Incorporated Oligonucleotides and methods for detecting hepatitis C viral nucleic acids
WO2003064701A2 (en) * 2002-01-30 2003-08-07 Epigenomics Ag Method for the analysis of cytosine methylation patterns
EP2112229A3 (de) 2002-11-25 2009-12-02 Sequenom, Inc. Verfahren zum Identifizieren des Brustkrebsrisikos und zugehörige Behandlungen
US9487823B2 (en) 2002-12-20 2016-11-08 Qiagen Gmbh Nucleic acid amplification
CN103396933B (zh) 2003-02-26 2016-04-20 凯利达基因组股份有限公司 通过杂交进行的随机阵列dna分析
US20040259128A1 (en) * 2003-03-24 2004-12-23 Glenn Kawasaki Methods and compositions for detecting the presence of target nucleic acids in a sample
US20040203005A1 (en) * 2003-04-14 2004-10-14 White Wanda L. B. Dual hybridization of complex nucleic acid samples for sequencing and single-nucleotide polymorphism identification
DE602004029694D1 (de) * 2003-08-11 2010-12-02 Univ Georgia State Res Found Rna-nachweis und quantifizierung
US20050153300A1 (en) * 2004-01-09 2005-07-14 Quest Diagnostics Incorporated Methods and compositions for the detection of mucolipidosis IV mutations
WO2005085475A1 (en) 2004-03-01 2005-09-15 Applera Corporation Methods, compositions and kits for use in polynucleotide amplification
US7575863B2 (en) * 2004-05-28 2009-08-18 Applied Biosystems, Llc Methods, compositions, and kits comprising linker probes for quantifying polynucleotides
US20060057595A1 (en) * 2004-09-16 2006-03-16 Applera Corporation Compositions, methods, and kits for identifying and quantitating small RNA molecules
US7879569B2 (en) 2004-11-09 2011-02-01 The Trustees Of The University Of Pennsylvania Methods for diagnosis of myelodysplastic syndromes (MDS)
WO2006081426A2 (en) * 2005-01-28 2006-08-03 Applera Corporation Compositions and methods for terminating a sequencing reaction at a specific location in a target dna template
JP2008535518A (ja) * 2005-04-14 2008-09-04 アプレラ コーポレイション プソイドイソシトシン核酸塩基誘導体を含む3’改変オリゴヌクレオチド、およびプライマーまたはプローブとしてのその適用
EP3257949A1 (de) 2005-06-15 2017-12-20 Complete Genomics Inc. Nukleinsäureanalyse durch zufällige mischungen von nichtüberlappenden fragmenten
US7892737B2 (en) * 2005-06-30 2011-02-22 Life Technologies Corporation Compositions, kits and methods pertaining to stability modulation of PNA oligomer/nucleic acid complexes
EP1762627A1 (de) 2005-09-09 2007-03-14 Qiagen GmbH Verfahren zur Aktivierung einer Nukleinsäure für eine Polymerase-Reaktion
US7960104B2 (en) * 2005-10-07 2011-06-14 Callida Genomics, Inc. Self-assembled single molecule arrays and uses thereof
CN101432439B (zh) 2006-02-24 2013-07-24 考利达基因组股份有限公司 Dna阵列上的高通量基因组测序
SG170028A1 (en) 2006-02-24 2011-04-29 Callida Genomics Inc High throughput genome sequencing on dna arrays
US7951572B2 (en) * 2006-02-27 2011-05-31 Korea Advanced Institute Of Science And Technology Construction of gold nanoparticle-based peptide chip, and assaying enzyme activity and inhibitor effect using secondary ion mass spectrometric analysis thereof
US8859752B2 (en) 2006-04-18 2014-10-14 The Trustees Of The University Of Pennsylvania SIRNA-based therapy of Fibrodyplasia Ossificans Progressiva (FOP)
CA2649770C (en) 2006-04-18 2017-08-15 The Trustees Of The University Of Pennsylvania Mutated acvr1 for diagnosis and treatment of fibrodysplasia ossificans progressiva (fop)
CA2651815A1 (en) * 2006-05-10 2007-11-22 Dxterity Diagnostics Detection of nucleic acid targets using chemically reactive oligonucleotide probes
EP3617321B1 (de) 2006-05-31 2024-10-23 Sequenom, Inc. Kit zur nukleinsäureextraktion und -verstärkung aus einer probe
CA2654165A1 (en) 2006-06-05 2007-12-13 Cancer Care Ontario Assessment of risk for colorectal cancer
EP2029726A4 (de) * 2006-06-09 2010-07-28 Univ Miami Beurteilung der zellenzusammensetzung und fraktionelle durchführbarkeit und verwendungen davon
US7910354B2 (en) 2006-10-27 2011-03-22 Complete Genomics, Inc. Efficient arrays of amplified polynucleotides
US20090111706A1 (en) * 2006-11-09 2009-04-30 Complete Genomics, Inc. Selection of dna adaptor orientation by amplification
US20080221832A1 (en) * 2006-11-09 2008-09-11 Complete Genomics, Inc. Methods for computing positional base probabilities using experminentals base value distributions
US20080131880A1 (en) * 2006-11-22 2008-06-05 Bortolin Laura T PNA-DNA oligomers and methods of use thereof
US7902345B2 (en) 2006-12-05 2011-03-08 Sequenom, Inc. Detection and quantification of biomolecules using mass spectrometry
US20080194416A1 (en) * 2007-02-08 2008-08-14 Sigma Aldrich Detection of mature small rna molecules
AU2008213634B2 (en) 2007-02-08 2013-09-05 Sequenom, Inc. Nucleic acid-based tests for RhD typing, gender determination and nucleic acid quantification
EP2243834A1 (de) 2007-03-05 2010-10-27 Cancer Care Ontario Abschätzung des Kolorektalkarzinomrisikos
US8652780B2 (en) 2007-03-26 2014-02-18 Sequenom, Inc. Restriction endonuclease enhanced polymorphic sequence detection
US8008010B1 (en) 2007-06-27 2011-08-30 Applied Biosystems, Llc Chimeric oligonucleotides for ligation-enhanced nucleic acid detection, methods and compositions therefor
WO2009032781A2 (en) 2007-08-29 2009-03-12 Sequenom, Inc. Methods and compositions for universal size-specific polymerase chain reaction
US20090061424A1 (en) * 2007-08-30 2009-03-05 Sigma-Aldrich Company Universal ligation array for analyzing gene expression or genomic variations
US8951731B2 (en) 2007-10-15 2015-02-10 Complete Genomics, Inc. Sequence analysis using decorated nucleic acids
US8415099B2 (en) 2007-11-05 2013-04-09 Complete Genomics, Inc. Efficient base determination in sequencing reactions
US7897344B2 (en) 2007-11-06 2011-03-01 Complete Genomics, Inc. Methods and oligonucleotide designs for insertion of multiple adaptors into library constructs
US8298768B2 (en) 2007-11-29 2012-10-30 Complete Genomics, Inc. Efficient shotgun sequencing methods
US8518640B2 (en) 2007-10-29 2013-08-27 Complete Genomics, Inc. Nucleic acid sequencing and process
WO2009061840A1 (en) 2007-11-05 2009-05-14 Complete Genomics, Inc. Methods and oligonucleotide designs for insertion of multiple adaptors employing selective methylation
US8592150B2 (en) 2007-12-05 2013-11-26 Complete Genomics, Inc. Methods and compositions for long fragment read sequencing
KR20090081260A (ko) * 2008-01-23 2009-07-28 삼성전자주식회사 마이크로 어레이 혼성화 검출 방법
WO2009094583A1 (en) * 2008-01-23 2009-07-30 Complete Genomics, Inc. Methods and compositions for preventing bias in amplification and sequencing reactions
WO2009097368A2 (en) * 2008-01-28 2009-08-06 Complete Genomics, Inc. Methods and compositions for efficient base calling in sequencing reactions
WO2009120808A2 (en) 2008-03-26 2009-10-01 Sequenom, Inc. Restriction endonuclease enhanced polymorphic sequence detection
WO2009132028A1 (en) * 2008-04-21 2009-10-29 Complete Genomics, Inc. Array structures for nucleic acid detection
JP5438922B2 (ja) * 2008-06-25 2014-03-12 日東電工株式会社 核酸の製造方法
US8476013B2 (en) 2008-09-16 2013-07-02 Sequenom, Inc. Processes and compositions for methylation-based acid enrichment of fetal nucleic acid from a maternal sample useful for non-invasive prenatal diagnoses
US8962247B2 (en) 2008-09-16 2015-02-24 Sequenom, Inc. Processes and compositions for methylation-based enrichment of fetal nucleic acid from a maternal sample useful for non invasive prenatal diagnoses
EP3722810A3 (de) 2009-02-11 2021-01-13 Caris MPI, Inc. Molekulare profilierung von tumoren
CA2757300C (en) 2009-04-01 2018-01-09 Dxterity Diagnostics Incorporated Chemical ligation dependent probe amplification (clpa)
EP3514244B1 (de) 2009-04-03 2021-07-07 Sequenom, Inc. Verfahren für die herstellung von nukleinsäuren
US9524369B2 (en) 2009-06-15 2016-12-20 Complete Genomics, Inc. Processing and analysis of complex nucleic acid sequence data
ES2521740T3 (es) * 2009-07-06 2014-11-13 Trilink Biotechnologies Cofactores, dadores y aceptores de ligasa modificados químicamente
US9631227B2 (en) 2009-07-06 2017-04-25 Trilink Biotechnologies, Inc. Chemically modified ligase cofactors, donors and acceptors
JP6013912B2 (ja) * 2009-07-30 2016-10-25 エフ.ホフマン−ラ ロシュ アーゲーF. Hoffmann−La Roche Aktiengesellschaft オリゴヌクレオチドプローブのセットならびにそれに関連する方法および使用
WO2011035135A1 (en) 2009-09-18 2011-03-24 The Trustees Of The University Of Pennsylvania Novel molecular beacons
EP2494077A4 (de) 2009-10-27 2013-08-21 Caris Mpi Inc Molekulare profilierung für personalisierte medizin
ES2577017T3 (es) 2009-12-22 2016-07-12 Sequenom, Inc. Procedimientos y kits para identificar la aneuploidia
US9404160B2 (en) 2009-12-22 2016-08-02 Becton, Dickinson And Company Methods for the detection of microorganisms
US9777332B2 (en) 2011-03-31 2017-10-03 St. Jude Children's Research Hospital Methods and compositions for identifying minimal residual disease in acute lymphoblastic leukemia
US9745616B2 (en) 2011-05-17 2017-08-29 Dxterity Diagnostics Incorporated Methods and compositions for detecting target nucleic acids
WO2013119845A1 (en) 2012-02-07 2013-08-15 Vibrant Holdings, Llc Substrates, peptide arrays, and methods
EP2820129A1 (de) 2012-03-02 2015-01-07 Sequenom, Inc. Verfahren und prozesse zur nichtinvasiven beurteilung genetischer variationen
KR101974577B1 (ko) * 2012-05-21 2019-05-02 삼성전자주식회사 나노입자 제작용 주형 및 이를 이용한 나노입자의 제조 방법
US9920361B2 (en) 2012-05-21 2018-03-20 Sequenom, Inc. Methods and compositions for analyzing nucleic acid
US10504613B2 (en) 2012-12-20 2019-12-10 Sequenom, Inc. Methods and processes for non-invasive assessment of genetic variations
US20140093873A1 (en) 2012-07-13 2014-04-03 Sequenom, Inc. Processes and compositions for methylation-based enrichment of fetal nucleic acid from a maternal sample useful for non-invasive prenatal diagnoses
US10006909B2 (en) 2012-09-28 2018-06-26 Vibrant Holdings, Llc Methods, systems, and arrays for biomolecular analysis
WO2014093291A1 (en) 2012-12-10 2014-06-19 Advandx, Inc. Use of probes for mass spectrometric identification of microorganisms or cells and associated conditions of interest
US9896728B2 (en) 2013-01-29 2018-02-20 Arcticrx Ltd. Method for determining a therapeutic approach for the treatment of age-related macular degeneration (AMD)
US9289502B2 (en) * 2013-03-08 2016-03-22 Emerald Therapeutics, Inc. Preparation of oligo conjugates
US11060145B2 (en) 2013-03-13 2021-07-13 Sequenom, Inc. Methods and compositions for identifying presence or absence of hypermethylation or hypomethylation locus
EP3736344A1 (de) 2014-03-13 2020-11-11 Sequenom, Inc. Verfahren und prozesse zur nichtinvasiven beurteilung genetischer variationen
CN106879252B (zh) 2014-06-10 2020-07-17 德克斯特里蒂诊断公司 用于收集和稳定生物样品的装置及方法
GB201612011D0 (en) 2016-07-11 2016-08-24 Glaxosmithkline Ip Dev Ltd Novel processes for the production of oligonucleotides
EP3299472A1 (de) * 2016-09-27 2018-03-28 Deutsches Krebsforschungszentrum Stiftung des Öffentlichen Rechts Verfahren zur markierung von oligonukleotidsonden
CN110736695A (zh) 2016-09-30 2020-01-31 多伦多大学管理委员会 用于鉴定和靶向异质群体中的个体细胞以选择性提取细胞内容物的系统
US10538808B2 (en) 2017-05-26 2020-01-21 Vibrant Holdings, Llc Photoactive compounds and methods for biomolecule detection and sequencing
BR112020021218A2 (pt) 2018-04-18 2021-03-02 St. Jude Children's Research Hospital ensaios de genotipagem para identificar mutações em xaf1
AU2019266303B2 (en) 2018-05-09 2025-12-18 Vibrant Holdings, Llc Methods of synthesizing a polynucleotide array using photactivated agents
EP3888021B1 (de) 2018-11-30 2024-02-21 Caris MPI, Inc. Molekulare profilierung der nächsten generation
CN114144525B (zh) * 2019-07-24 2024-11-12 国立大学法人东北大学 嵌合分子、药物组合物、靶核酸的切割方法以及靶核酸切割用或诊断用试剂盒
CA3163319A1 (en) 2019-12-02 2021-06-10 Caris Mpi, Inc. Pan-cancer platinum response predictor
WO2023069429A1 (en) * 2021-10-19 2023-04-27 Oncogenuity, Inc. Peptide nucleic acid compositions and methods of use for gene editing

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU7661794A (en) 1993-09-21 1995-04-10 Amersham International Plc Reagents comprising chimeric molecules of nucleic acids and nucleic acid analogs
GB9507238D0 (en) * 1995-04-07 1995-05-31 Isis Innovation Detecting dna sequence variations
DE19637339A1 (de) 1996-09-13 1998-03-19 Hoechst Ag Verfahren zur Amplifikation von Nukleinsäuren
WO1999049293A2 (en) * 1998-03-24 1999-09-30 Boston Probes, Inc. Methods, kits and compositions pertaining to detection complexes

Also Published As

Publication number Publication date
US20020177133A1 (en) 2002-11-28
JP3789817B2 (ja) 2006-06-28
US6469151B1 (en) 2002-10-22
JP2003511059A (ja) 2003-03-25
EP1220953A2 (de) 2002-07-10
WO2001027326A3 (en) 2002-05-10
CA2353801A1 (en) 2001-04-19
EP1220953B1 (de) 2004-12-22
DE60016947D1 (de) 2005-01-27
US6297016B1 (en) 2001-10-02
AU768240B2 (en) 2003-12-04
AU7871200A (en) 2001-04-23
JP2006055177A (ja) 2006-03-02
DE60016947T2 (de) 2005-12-15
WO2001027326A2 (en) 2001-04-19

Similar Documents

Publication Publication Date Title
ATE285479T1 (de) Template abhängige ligierung mit pna-dna chimerischen sonden
WO2001012852A3 (en) Polymerase extension at 3' terminus of pna-dna chimera
US20110014617A1 (en) Methods and Systems for Detecting Nucleic Acids
EP2366803A3 (de) Zusammensetzungen und Verfahren zum Nacheis des West Nil Viruses.
WO2003043402A3 (en) Nucleic acid probes and methods to detect and/or quantify nucleic acid analytes
CA2308599A1 (en) Dna polymorphism identity determination using flow cytometry
ATE427999T1 (de) Erkennung von nukleinsaurepolymorphismen
JPH08211050A (ja) 特定核酸配列の検出方法
JP2005521409A (ja) 単一プライマー等温核酸増幅−増強型被分析物検出および定量
CA2039517A1 (en) Dna probe signal amplification
JP2005502346A (ja) 核酸配列の非特異的ハイブリダイゼーションをブロックするための方法
ATE340866T1 (de) Zusammensetzungen und verfahren für die gleichzeitige detektion und quantifizierung von einer mehrheit spezifischer nuklein säure sequenzen
JP2000514307A (ja) 増加した標的特異的t▲下m▼を持つ修飾オリゴヌクレオチドを用いた核酸配列の検出および増幅のための方法
US20080193940A1 (en) Systems and methods for detecting nucleic acids
DE69815370D1 (de) Fluoreszend-konjugaten von nukleosiden und von nukleotiden, ihrer verfahren zur herstellung und ihrer verwendungen
WO2010113088A1 (en) Methylation ligation-dependent macroarray (mlm)
US20150111774A1 (en) Method for Detecting Target Nucleic Acid
CN1141399C (zh) 用于均相荧光pcr检测的孪生引物
EP3633049A1 (de) Neuartige fluoreszenzlöschungssonde zur messung von nukleinsäure
US20120196765A1 (en) Method for detection or analysis of target sequence in genomic dna
ATE287969T1 (de) Amplifizierungsverfahen zum nachweis von zielnukleinsäuren unter verwendung von fluoreszenzenergieübertragung
US7491492B2 (en) Method of detecting nucleotide mutations
CN115976179B (zh) 一种高灵敏度探针及其制备方法和应用
US8808993B2 (en) Methods and kits to detect new H1N1 “swine flu” variants
JPWO2014156513A1 (ja) 変異の検出方法

Legal Events

Date Code Title Description
RER Ceased as to paragraph 5 lit. 3 law introducing patent treaties