ATE286144T1 - VERWENDUNG VON FLUORESZIERENDEN ßMOLECULAR BEACONSß ZUM NACHWEIS VON METHYLIERTEN NUKLEINSÄUREN - Google Patents
VERWENDUNG VON FLUORESZIERENDEN ßMOLECULAR BEACONSß ZUM NACHWEIS VON METHYLIERTEN NUKLEINSÄURENInfo
- Publication number
- ATE286144T1 ATE286144T1 AT00904696T AT00904696T ATE286144T1 AT E286144 T1 ATE286144 T1 AT E286144T1 AT 00904696 T AT00904696 T AT 00904696T AT 00904696 T AT00904696 T AT 00904696T AT E286144 T1 ATE286144 T1 AT E286144T1
- Authority
- AT
- Austria
- Prior art keywords
- nucleic acid
- stem
- region
- nucleotides
- nucleic acids
- Prior art date
Links
- 108020004707 nucleic acids Proteins 0.000 title abstract 5
- 150000007523 nucleic acids Chemical class 0.000 title abstract 5
- 102000039446 nucleic acids Human genes 0.000 title abstract 5
- 125000003729 nucleotide group Chemical group 0.000 abstract 4
- 239000000523 sample Substances 0.000 abstract 4
- 239000002773 nucleotide Substances 0.000 abstract 3
- 108091034117 Oligonucleotide Proteins 0.000 abstract 2
- 108020005187 Oligonucleotide Probes Proteins 0.000 abstract 1
- 230000000295 complement effect Effects 0.000 abstract 1
- 238000009396 hybridization Methods 0.000 abstract 1
- 238000000034 method Methods 0.000 abstract 1
- 230000011987 methylation Effects 0.000 abstract 1
- 238000007069 methylation reaction Methods 0.000 abstract 1
- 238000007899 nucleic acid hybridization Methods 0.000 abstract 1
- 239000002751 oligonucleotide probe Substances 0.000 abstract 1
- 238000010791 quenching Methods 0.000 abstract 1
- 230000000171 quenching effect Effects 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
- C12Q1/6886—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material for cancer
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6816—Hybridisation assays characterised by the detection means
- C12Q1/6818—Hybridisation assays characterised by the detection means involving interaction of two or more labels, e.g. resonant energy transfer
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/154—Methylation markers
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- Immunology (AREA)
- Analytical Chemistry (AREA)
- Genetics & Genomics (AREA)
- Biochemistry (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Pathology (AREA)
- Hospice & Palliative Care (AREA)
- Oncology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| AUPP8448A AUPP844899A0 (en) | 1999-02-01 | 1999-02-01 | A method for detecting methylated nucleic acids |
| PCT/AU2000/000053 WO2000046398A1 (en) | 1999-02-01 | 2000-02-01 | Use of fluorescent molecular beacons in the detection of methylated nucleic acids |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ATE286144T1 true ATE286144T1 (de) | 2005-01-15 |
Family
ID=3812648
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AT00904696T ATE286144T1 (de) | 1999-02-01 | 2000-02-01 | VERWENDUNG VON FLUORESZIERENDEN ßMOLECULAR BEACONSß ZUM NACHWEIS VON METHYLIERTEN NUKLEINSÄUREN |
Country Status (9)
| Country | Link |
|---|---|
| US (2) | US20030017465A1 (de) |
| EP (1) | EP1151138B1 (de) |
| JP (1) | JP2002535998A (de) |
| AT (1) | ATE286144T1 (de) |
| AU (1) | AUPP844899A0 (de) |
| CA (1) | CA2361654A1 (de) |
| DE (1) | DE60017089T2 (de) |
| ES (1) | ES2235832T3 (de) |
| WO (1) | WO2000046398A1 (de) |
Families Citing this family (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6960436B2 (en) * | 2002-02-06 | 2005-11-01 | Epigenomics Ag | Quantitative methylation detection in DNA samples |
| DE10304219B3 (de) * | 2003-01-30 | 2004-08-19 | Epigenomics Ag | Verfahren zum Nachweis von Cytosin-Methylierungsmustern mit hoher Sensitivität |
| US7741031B2 (en) * | 2003-03-07 | 2010-06-22 | The Public Health Research Institute Of The City Of New York | Optically decodable microcarries, arrays and methods |
| DE10338308B4 (de) * | 2003-08-15 | 2006-10-19 | Epigenomics Ag | Verfahren zum Nachweis von Cytosin-Methylierungen in DNA |
| WO2005098036A1 (en) * | 2004-04-12 | 2005-10-20 | Postech Foundation | Oligonucleotide for detecting target dna or rna |
| GB0714058D0 (en) * | 2007-07-19 | 2007-08-29 | Abertec Ltd | detection of methylation in nucleic acid sequences |
| US9393566B2 (en) | 2008-06-23 | 2016-07-19 | Canon U.S. Life Sciences, Inc. | System and method for temperature referencing for melt curve data collection |
| US8691504B2 (en) | 2009-05-26 | 2014-04-08 | Xiamen University | Method for detecting variations in nucleic acid sequences |
| CN102051408B (zh) * | 2009-11-04 | 2012-07-04 | 高新 | 用于前列腺癌转移早期诊断的试剂盒 |
| EP2865764B1 (de) * | 2012-06-20 | 2018-09-05 | Toray Industries, Inc. | Verfahren zum nachweis von nukleinsäure und verwendung eines kits zum nachweis von nukleinsäure |
| GB201402644D0 (en) | 2014-02-14 | 2014-04-02 | Base4 Innovation Ltd | Methylation detection method |
| KR102923870B1 (ko) | 2019-09-02 | 2026-02-06 | 주식회사 시선바이오머티리얼스 | 신규한 pna 올리고머, 이의 dna 메틸화 검출용도 및 이를 이용한 dna 메틸화 검출방법 |
| WO2023278744A1 (en) * | 2021-07-02 | 2023-01-05 | Illumina, Inc. | Detecting methylcytosine using a modified base opposite to the methylcytosine |
| CN113552191B (zh) * | 2021-07-28 | 2023-11-21 | 江苏师范大学 | 基于多层dna放大回路检测甲基化dna的比例型电化学传感器的构建方法 |
Family Cites Families (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5426180A (en) * | 1991-03-27 | 1995-06-20 | Research Corporation Technologies, Inc. | Methods of making single-stranded circular oligonucleotides |
| US5808036A (en) * | 1993-09-01 | 1998-09-15 | Research Corporation Technologies Inc. | Stem-loop oligonucleotides containing parallel and antiparallel binding domains |
| AU713667B2 (en) * | 1996-04-12 | 1999-12-09 | Phri Properties, Inc. | Detection probes, kits and assays |
| US6017704A (en) * | 1996-06-03 | 2000-01-25 | The Johns Hopkins University School Of Medicine | Method of detection of methylated nucleic acid using agents which modify unmethylated cytosine and distinguishing modified methylated and non-methylated nucleic acids |
| US6117635A (en) * | 1996-07-16 | 2000-09-12 | Intergen Company | Nucleic acid amplification oligonucleotides with molecular energy transfer labels and methods based thereon |
| AU7847798A (en) * | 1996-12-13 | 1998-07-03 | Government Of The United States Of America, As Represented By The Secretary Of The Department Of Health And Human Services, The | Fluorescent nucleotide analog hairpin formation for detection of nuclei c acid hybridization |
| EP1025120B1 (de) * | 1997-10-27 | 2010-08-18 | Boston Probes, Inc. | Sich auf "pna molecular beacons" beziehende verfahren, testsätze und zusammensetzungen |
| GB9725237D0 (en) * | 1997-11-29 | 1998-01-28 | Secr Defence | Amplification system |
| AU6414199A (en) * | 1998-10-06 | 2000-04-26 | Emory University | Molecular diagnostics for galactosemia |
-
1999
- 1999-02-01 AU AUPP8448A patent/AUPP844899A0/en not_active Abandoned
-
2000
- 2000-02-01 WO PCT/AU2000/000053 patent/WO2000046398A1/en not_active Ceased
- 2000-02-01 ES ES00904696T patent/ES2235832T3/es not_active Expired - Lifetime
- 2000-02-01 EP EP00904696A patent/EP1151138B1/de not_active Expired - Lifetime
- 2000-02-01 AT AT00904696T patent/ATE286144T1/de not_active IP Right Cessation
- 2000-02-01 CA CA002361654A patent/CA2361654A1/en not_active Abandoned
- 2000-02-01 DE DE60017089T patent/DE60017089T2/de not_active Expired - Lifetime
- 2000-02-01 JP JP2000597457A patent/JP2002535998A/ja active Pending
-
2001
- 2001-07-31 US US09/920,000 patent/US20030017465A1/en not_active Abandoned
-
2003
- 2003-07-10 US US10/617,060 patent/US20050221329A1/en not_active Abandoned
Also Published As
| Publication number | Publication date |
|---|---|
| US20050221329A1 (en) | 2005-10-06 |
| DE60017089D1 (de) | 2005-02-03 |
| EP1151138A1 (de) | 2001-11-07 |
| WO2000046398A1 (en) | 2000-08-10 |
| EP1151138B1 (de) | 2004-12-29 |
| EP1151138A4 (de) | 2003-01-22 |
| DE60017089T2 (de) | 2006-01-05 |
| US20030017465A1 (en) | 2003-01-23 |
| CA2361654A1 (en) | 2000-08-10 |
| ES2235832T3 (es) | 2005-07-16 |
| AUPP844899A0 (en) | 1999-02-25 |
| JP2002535998A (ja) | 2002-10-29 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| RER | Ceased as to paragraph 5 lit. 3 law introducing patent treaties |