ATE290210T1 - Verwendung von antikörpern gegen embryonales hämoglobin zur identifizierung von fötalen zellen - Google Patents
Verwendung von antikörpern gegen embryonales hämoglobin zur identifizierung von fötalen zellenInfo
- Publication number
- ATE290210T1 ATE290210T1 AT97911938T AT97911938T ATE290210T1 AT E290210 T1 ATE290210 T1 AT E290210T1 AT 97911938 T AT97911938 T AT 97911938T AT 97911938 T AT97911938 T AT 97911938T AT E290210 T1 ATE290210 T1 AT E290210T1
- Authority
- AT
- Austria
- Prior art keywords
- fetal
- fetal cells
- proteins
- determining
- antibodies
- Prior art date
Links
- 230000001605 fetal effect Effects 0.000 title abstract 6
- 102000001554 Hemoglobins Human genes 0.000 title abstract 3
- 108010054147 Hemoglobins Proteins 0.000 title abstract 3
- 108090000623 proteins and genes Proteins 0.000 abstract 4
- 102000004169 proteins and genes Human genes 0.000 abstract 4
- 150000007523 nucleic acids Chemical class 0.000 abstract 3
- 108091005461 Nucleic proteins Proteins 0.000 abstract 2
- 210000004027 cell Anatomy 0.000 abstract 2
- 210000003924 normoblast Anatomy 0.000 abstract 2
- 102000039446 nucleic acids Human genes 0.000 abstract 2
- 108020004707 nucleic acids Proteins 0.000 abstract 2
- 102000008394 Immunoglobulin Fragments Human genes 0.000 abstract 1
- 108010021625 Immunoglobulin Fragments Proteins 0.000 abstract 1
- 230000005856 abnormality Effects 0.000 abstract 1
- 230000003321 amplification Effects 0.000 abstract 1
- 210000004369 blood Anatomy 0.000 abstract 1
- 239000008280 blood Substances 0.000 abstract 1
- 230000002759 chromosomal effect Effects 0.000 abstract 1
- 238000011156 evaluation Methods 0.000 abstract 1
- 210000003754 fetus Anatomy 0.000 abstract 1
- 238000000338 in vitro Methods 0.000 abstract 1
- 238000000034 method Methods 0.000 abstract 1
- 238000003199 nucleic acid amplification method Methods 0.000 abstract 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
- G01N33/689—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids related to pregnancy or the gonads
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N15/14—Optical investigation techniques, e.g. flow cytometry
- G01N15/1456—Optical investigation techniques, e.g. flow cytometry without spatial resolution of the texture or inner structure of the particle, e.g. processing of pulse signals
- G01N15/1459—Optical investigation techniques, e.g. flow cytometry without spatial resolution of the texture or inner structure of the particle, e.g. processing of pulse signals the analysis being performed on a sample stream
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2800/00—Detection or diagnosis of diseases
- G01N2800/36—Gynecology or obstetrics
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2800/00—Detection or diagnosis of diseases
- G01N2800/36—Gynecology or obstetrics
- G01N2800/368—Pregnancy complicated by disease or abnormalities of pregnancy, e.g. preeclampsia, preterm labour
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S435/00—Chemistry: molecular biology and microbiology
- Y10S435/975—Kit
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Immunology (AREA)
- Urology & Nephrology (AREA)
- Biomedical Technology (AREA)
- Hematology (AREA)
- Molecular Biology (AREA)
- Physics & Mathematics (AREA)
- Pathology (AREA)
- General Physics & Mathematics (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Analytical Chemistry (AREA)
- Food Science & Technology (AREA)
- Microbiology (AREA)
- Medicinal Chemistry (AREA)
- Biotechnology (AREA)
- Cell Biology (AREA)
- Pregnancy & Childbirth (AREA)
- Reproductive Health (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Gynecology & Obstetrics (AREA)
- Dispersion Chemistry (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US08/734,556 US5731156A (en) | 1996-10-21 | 1996-10-21 | Use of anti-embryonic hemoglobin antibodies to identify fetal cells |
| PCT/US1997/019447 WO1998018005A1 (en) | 1996-10-21 | 1997-10-20 | Use of anti-embryonic hemoglobin antibodies to identify fetal cells |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ATE290210T1 true ATE290210T1 (de) | 2005-03-15 |
Family
ID=24952172
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AT97911938T ATE290210T1 (de) | 1996-10-21 | 1997-10-20 | Verwendung von antikörpern gegen embryonales hämoglobin zur identifizierung von fötalen zellen |
Country Status (13)
| Country | Link |
|---|---|
| US (1) | US5731156A (de) |
| EP (1) | EP1007965B1 (de) |
| JP (1) | JP4091123B2 (de) |
| KR (1) | KR100523381B1 (de) |
| CN (1) | CN1234117A (de) |
| AT (1) | ATE290210T1 (de) |
| AU (1) | AU735380B2 (de) |
| BR (1) | BR9712548A (de) |
| CA (1) | CA2269327A1 (de) |
| DE (1) | DE69732662T2 (de) |
| IL (1) | IL129382A (de) |
| RU (1) | RU2178703C2 (de) |
| WO (1) | WO1998018005A1 (de) |
Families Citing this family (76)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6509192B1 (en) * | 1992-02-24 | 2003-01-21 | Coulter International Corp. | Quality control method |
| US5629147A (en) * | 1992-07-17 | 1997-05-13 | Aprogenex, Inc. | Enriching and identifying fetal cells in maternal blood for in situ hybridization |
| DE69627183T2 (de) | 1995-11-30 | 2004-01-29 | Chromavision Med Sys Inc | Verfahren zur automatischen bildanalyse biologischer proben |
| US6718053B1 (en) * | 1996-11-27 | 2004-04-06 | Chromavision Medical Systems, Inc. | Method and apparatus for automated image analysis of biological specimens |
| JP2001514751A (ja) * | 1997-03-08 | 2001-09-11 | ザ・ユニバーシティー・オブ・ダンディー | 胎児診断方法 |
| US5962234A (en) * | 1997-10-20 | 1999-10-05 | Applied Imaging Corporation | Use of anti-embryonic epsilon hemoglobin antibodies to identify fetal cells |
| JP2002514762A (ja) * | 1998-05-09 | 2002-05-21 | アイコニシス，インコーポレーテッド | コンピュータによって制御された、胎児細胞を含む希少細胞に基づく診断のための方法および装置 |
| FR2781055B1 (fr) * | 1998-07-09 | 2000-10-13 | Immunotech Sa | Reactif et methode pour la permeabilisation et l'identification des erythrocytes |
| WO2000071987A2 (en) * | 1999-05-25 | 2000-11-30 | John Philip | Isolation and culturing of fetal cells |
| US6692952B1 (en) * | 1999-11-10 | 2004-02-17 | Massachusetts Institute Of Technology | Cell analysis and sorting apparatus for manipulation of cells |
| DE10035433C2 (de) * | 2000-07-20 | 2002-07-18 | Tuma Wolfgang | Schonende Hochanreicherung von fetalen Zellen aus pripherem Blut und Verwendung derselben |
| JP4229237B2 (ja) * | 2001-12-11 | 2009-02-25 | 株式会社ネーテック | 血液細胞分離システム |
| EP2359689B1 (de) | 2002-09-27 | 2015-08-26 | The General Hospital Corporation | Mikrofluid-Vorrichtung zur Zelltrennung, sowie Verwendung derselben |
| CA2445204C (en) | 2002-10-16 | 2014-08-12 | Streck Laboratories, Inc. | Method and device for collecting and preserving cells for analysis |
| US20040214243A1 (en) * | 2003-04-25 | 2004-10-28 | Beckman Coulter, Inc. | Differential determination of hemoglobins |
| EP1636564A1 (de) * | 2003-06-13 | 2006-03-22 | The General Hospital Corporation | Mikrofluidsysteme zur abtrennung roter blutkörperchen und von blutplättchen nach grösse aus blut |
| US20050266433A1 (en) * | 2004-03-03 | 2005-12-01 | Ravi Kapur | Magnetic device for isolation of cells and biomolecules in a microfluidic environment |
| US7653260B2 (en) * | 2004-06-17 | 2010-01-26 | Carl Zeis MicroImaging GmbH | System and method of registering field of view |
| US8582924B2 (en) * | 2004-06-30 | 2013-11-12 | Carl Zeiss Microimaging Gmbh | Data structure of an image storage and retrieval system |
| AU2005282720B2 (en) * | 2004-09-02 | 2011-08-04 | Genentech, Inc. | Anti-FC-gamma RIIB receptor antibody and uses therefor |
| US20070026415A1 (en) * | 2005-07-29 | 2007-02-01 | Martin Fuchs | Devices and methods for enrichment and alteration of circulating tumor cells and other particles |
| US20070196820A1 (en) | 2005-04-05 | 2007-08-23 | Ravi Kapur | Devices and methods for enrichment and alteration of cells and other particles |
| US20070026414A1 (en) * | 2005-07-29 | 2007-02-01 | Martin Fuchs | Devices and methods for enrichment and alteration of circulating tumor cells and other particles |
| US20070026417A1 (en) * | 2005-07-29 | 2007-02-01 | Martin Fuchs | Devices and methods for enrichment and alteration of circulating tumor cells and other particles |
| US20070026413A1 (en) * | 2005-07-29 | 2007-02-01 | Mehmet Toner | Devices and methods for enrichment and alteration of circulating tumor cells and other particles |
| US20070059680A1 (en) * | 2005-09-15 | 2007-03-15 | Ravi Kapur | System for cell enrichment |
| US8921102B2 (en) | 2005-07-29 | 2014-12-30 | Gpb Scientific, Llc | Devices and methods for enrichment and alteration of circulating tumor cells and other particles |
| US20070026416A1 (en) * | 2005-07-29 | 2007-02-01 | Martin Fuchs | Devices and methods for enrichment and alteration of circulating tumor cells and other particles |
| US20090181421A1 (en) * | 2005-07-29 | 2009-07-16 | Ravi Kapur | Diagnosis of fetal abnormalities using nucleated red blood cells |
| US20070059718A1 (en) * | 2005-09-15 | 2007-03-15 | Mehmet Toner | Systems and methods for enrichment of analytes |
| US20070059716A1 (en) * | 2005-09-15 | 2007-03-15 | Ulysses Balis | Methods for detecting fetal abnormality |
| US20070059781A1 (en) * | 2005-09-15 | 2007-03-15 | Ravi Kapur | System for size based separation and analysis |
| US20070059774A1 (en) * | 2005-09-15 | 2007-03-15 | Michael Grisham | Kits for Prenatal Testing |
| US20070059719A1 (en) * | 2005-09-15 | 2007-03-15 | Michael Grisham | Business methods for prenatal Diagnosis |
| US20070059683A1 (en) * | 2005-09-15 | 2007-03-15 | Tom Barber | Veterinary diagnostic system |
| CA2690612C (en) | 2007-05-14 | 2016-07-05 | Historx, Inc. | Compartment segregation by pixel characterization using image data clustering |
| ES2599902T3 (es) * | 2007-06-15 | 2017-02-06 | Novartis Ag | Sistema y método de microscopio para obtener datos de muestra normalizados |
| CA2604317C (en) | 2007-08-06 | 2017-02-28 | Historx, Inc. | Methods and system for validating sample images for quantitative immunoassays |
| CA2596204C (en) * | 2007-08-07 | 2019-02-26 | Historx, Inc. | Method and system for determining an optimal dilution of a reagent |
| WO2009029810A1 (en) * | 2007-08-31 | 2009-03-05 | Historx, Inc. | Automatic exposure time selection for imaging tissue |
| EP2232266B1 (de) | 2007-12-20 | 2012-12-05 | Cytyc Corporation | Verfahren zur magnetischen trennung von roten blutzellen aus einer patientenprobe |
| CA2752838A1 (en) * | 2008-02-18 | 2009-08-27 | Genetic Technologies Limited | Cell processing and/or enrichment methods |
| US20100159506A1 (en) * | 2008-07-25 | 2010-06-24 | Cellscape Corporation | Methods and systems for genetic analysis of fetal nucleated red blood cells |
| JP5551702B2 (ja) * | 2008-09-16 | 2014-07-16 | ヒストロックス，インコーポレイテッド． | バイオマーカー発現の再現性のある定量 |
| WO2010078194A1 (en) * | 2008-12-30 | 2010-07-08 | Streck, Inc. | Method for screening blood using a preservative that may be in a substantially solid state form |
| US11634747B2 (en) * | 2009-01-21 | 2023-04-25 | Streck Llc | Preservation of fetal nucleic acids in maternal plasma |
| EP2389455A4 (de) * | 2009-01-26 | 2012-12-05 | Verinata Health Inc | Verfahren und zusammensetzung zur identifizierung einer fetalen zelle |
| US8231007B2 (en) * | 2009-01-29 | 2012-07-31 | Wark Rickey E | Static classifier cage |
| NO2398912T3 (de) | 2009-02-18 | 2018-02-10 | ||
| WO2010121294A1 (en) * | 2009-04-21 | 2010-10-28 | Genetic Technologies Limited | Methods for obtaining fetal genetic material |
| EP2446267A1 (de) * | 2009-06-24 | 2012-05-02 | Health Corporation - Rambam | Verfahren und kits zur isolation von plazentalen mikropartikeln und ihre verwendung zur diagnose von fetalen erkrankungen |
| US20110111410A1 (en) * | 2009-11-09 | 2011-05-12 | Streck, Inc. | Stabilization of rna in intact cells within a blood sample |
| BR112012018166A2 (pt) | 2010-01-21 | 2015-09-15 | Biocep Ltd | separação magnética de células raras. |
| US8774488B2 (en) | 2010-03-11 | 2014-07-08 | Cellscape Corporation | Method and device for identification of nucleated red blood cells from a maternal blood sample |
| CA2796087A1 (en) * | 2010-04-20 | 2011-10-27 | Ventana Medical Systems, Inc. | Two-color chromogenic in situ hybridization |
| US9956281B2 (en) | 2011-05-04 | 2018-05-01 | Streck, Inc. | Inactivated virus compositions and methods of preparing such compositions |
| AU2012358810B2 (en) * | 2011-12-23 | 2018-03-15 | DePuy Synthes Products, Inc. | Detection of human umbilical cord tissue-derived cells |
| CN104508122A (zh) * | 2012-06-20 | 2015-04-08 | 赛璐拉有限公司 | 用于处理细胞群体的材料和方法 |
| CA2876692C (en) | 2012-06-22 | 2022-05-24 | Quantrx Biomedical Corporation | Method for obtaining fetal cells and fetal cellular components |
| CN109554350B (zh) * | 2012-11-27 | 2022-09-23 | 儿童医疗中心有限公司 | 用于胎儿血红蛋白再诱导的靶向bcl11a远端调控元件 |
| JP2016521353A (ja) * | 2013-03-27 | 2016-07-21 | セラノス， インコーポレイテッド | サンプル分析のための方法、機器、及びシステム |
| US10091984B2 (en) | 2013-07-24 | 2018-10-09 | Streck, Inc. | Compositions and methods for stabilizing circulating tumor cells |
| JP2017521091A (ja) * | 2014-05-15 | 2017-08-03 | ケルベンクス インコーポレイテッド | 診断試験用の胎児有核赤血球（ｎｒｂｃ）の調製 |
| US11168351B2 (en) | 2015-03-05 | 2021-11-09 | Streck, Inc. | Stabilization of nucleic acids in urine |
| CN104977284B (zh) * | 2015-07-03 | 2018-05-29 | 石莹 | 一种胎儿有核红细胞的捕获及鉴定方法 |
| KR101794218B1 (ko) * | 2015-08-19 | 2017-11-07 | 인제대학교 산학협력단 | 비침습적 산전 진단을 위한 산모 혈액 내 유핵 적혈구의 농축 분리 방법 |
| WO2017057076A1 (ja) * | 2015-09-28 | 2017-04-06 | 富士フイルム株式会社 | 有核赤血球の取得方法および有核赤血球の識別方法 |
| US20170145475A1 (en) | 2015-11-20 | 2017-05-25 | Streck, Inc. | Single spin process for blood plasma separation and plasma composition including preservative |
| WO2018022991A1 (en) | 2016-07-29 | 2018-02-01 | Streck, Inc. | Suspension composition for hematology analysis control |
| JP6234542B1 (ja) | 2016-12-27 | 2017-11-22 | 株式会社 TL Genomics | 胎児細胞由来染色体ｄｎａの取得方法 |
| CN115119829B (zh) | 2017-10-19 | 2024-07-12 | 斯特雷克股份有限公司 | 用于胞外囊泡的溶血和凝血调节以及稳定化的组合物 |
| CA3076601C (en) | 2017-10-19 | 2022-08-16 | Tl Genomics Inc. | Chip for cell classification |
| CN109115584A (zh) * | 2018-09-10 | 2019-01-01 | 首都医科大学附属北京妇产医院 | 一种检测红细胞中血红蛋白f的检测试剂及其应用 |
| AU2020315211A1 (en) * | 2019-07-15 | 2022-02-10 | A. Menarini Biomarkers Singapore Pte. Ltd. | Compositions and methods for isolating, detecting, and analyzing fetal cells |
| CN111948404B (zh) * | 2020-08-03 | 2023-04-07 | 融智生物科技（青岛）有限公司 | 用于筛查地中海贫血的特征蛋白标记组合物、质谱模型及其应用 |
| CN116183469B (zh) * | 2022-12-16 | 2025-12-02 | 河北明志康华生物科技有限公司 | 一种胎儿红细胞流式细胞检测的方法和应用 |
Family Cites Families (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH05501612A (ja) * | 1989-11-13 | 1993-03-25 | チルドレンズ・メデイカル・センター・コーポレーシヨン | 胎児ｄｎａ単離および検出のための非侵入性方法 |
| BR9306867A (pt) * | 1992-07-17 | 1998-12-08 | Aprogenex Inc | Enriquecimento e identificação de células fetais do sangue materno para a hibridização in situ |
| US5457024A (en) * | 1993-01-22 | 1995-10-10 | Aprogenex, Inc. | Isolation of fetal erythrocytes |
| AU7474394A (en) * | 1993-07-19 | 1995-02-20 | Aprogenex, Inc. | Enriching and identifying fetal cells in maternal blood for in situ hybridization |
| US5580724A (en) * | 1994-03-25 | 1996-12-03 | Board Of Regents, The University Of Texas System | Differential expansion of fetal stem cells in maternal circulation for use in prenatal genetic analysis |
-
1996
- 1996-10-21 US US08/734,556 patent/US5731156A/en not_active Expired - Lifetime
-
1997
- 1997-10-20 RU RU99110373/14A patent/RU2178703C2/ru not_active IP Right Cessation
- 1997-10-20 DE DE69732662T patent/DE69732662T2/de not_active Expired - Fee Related
- 1997-10-20 EP EP97911938A patent/EP1007965B1/de not_active Expired - Lifetime
- 1997-10-20 AU AU49200/97A patent/AU735380B2/en not_active Ceased
- 1997-10-20 CA CA002269327A patent/CA2269327A1/en not_active Abandoned
- 1997-10-20 WO PCT/US1997/019447 patent/WO1998018005A1/en not_active Ceased
- 1997-10-20 BR BR9712548-2A patent/BR9712548A/pt not_active Application Discontinuation
- 1997-10-20 CN CN97199005A patent/CN1234117A/zh active Pending
- 1997-10-20 IL IL12938297A patent/IL129382A/en not_active IP Right Cessation
- 1997-10-20 JP JP51969498A patent/JP4091123B2/ja not_active Expired - Fee Related
- 1997-10-20 AT AT97911938T patent/ATE290210T1/de not_active IP Right Cessation
- 1997-10-20 KR KR10-1999-7003439A patent/KR100523381B1/ko not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| EP1007965A4 (de) | 2000-06-14 |
| IL129382A (en) | 2002-09-12 |
| WO1998018005A1 (en) | 1998-04-30 |
| JP2001502805A (ja) | 2001-02-27 |
| RU2178703C2 (ru) | 2002-01-27 |
| EP1007965B1 (de) | 2005-03-02 |
| DE69732662T2 (de) | 2005-12-29 |
| BR9712548A (pt) | 1999-12-21 |
| AU4920097A (en) | 1998-05-15 |
| AU735380B2 (en) | 2001-07-05 |
| JP4091123B2 (ja) | 2008-05-28 |
| KR100523381B1 (ko) | 2005-10-24 |
| EP1007965A1 (de) | 2000-06-14 |
| IL129382A0 (en) | 2000-02-17 |
| HK1031255A1 (en) | 2001-06-08 |
| KR20000052662A (ko) | 2000-08-25 |
| CN1234117A (zh) | 1999-11-03 |
| DE69732662D1 (de) | 2005-04-07 |
| CA2269327A1 (en) | 1998-04-30 |
| US5731156A (en) | 1998-03-24 |
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