ATE296310T1 - Syntheseverfahren zum ökonomischen aufbau langer dna-sequenzen und zusammensetzungen hierfür - Google Patents

Syntheseverfahren zum ökonomischen aufbau langer dna-sequenzen und zusammensetzungen hierfür

Info

Publication number
ATE296310T1
ATE296310T1 AT00914914T AT00914914T ATE296310T1 AT E296310 T1 ATE296310 T1 AT E296310T1 AT 00914914 T AT00914914 T AT 00914914T AT 00914914 T AT00914914 T AT 00914914T AT E296310 T1 ATE296310 T1 AT E296310T1
Authority
AT
Austria
Prior art keywords
dna sequences
synthesis process
long dna
economical construction
compositions therefor
Prior art date
Application number
AT00914914T
Other languages
English (en)
Inventor
Thomas M Brennan
Herbert L Heyneker
Original Assignee
Metrigen Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Metrigen Inc filed Critical Metrigen Inc
Application granted granted Critical
Publication of ATE296310T1 publication Critical patent/ATE296310T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C12N15/1031—Mutagenizing nucleic acids mutagenesis by gene assembly, e.g. assembly by oligonucleotide extension PCR
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J19/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J19/0046—Sequential or parallel reactions, e.g. for the synthesis of polypeptides or polynucleotides; Apparatus and devices for combinatorial chemistry or for making molecular arrays
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/66—General methods for inserting a gene into a vector to form a recombinant vector using cleavage and ligation; Use of non-functional linkers or adaptors, e.g. linkers containing the sequence for a restriction endonuclease
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00277—Apparatus
    • B01J2219/00351—Means for dispensing and evacuation of reagents
    • B01J2219/00378—Piezoelectric or ink jet dispensers
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00277—Apparatus
    • B01J2219/00497—Features relating to the solid phase supports
    • B01J2219/00527—Sheets
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00585—Parallel processes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/0059—Sequential processes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00596—Solid-phase processes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00603—Making arrays on substantially continuous surfaces
    • B01J2219/00605—Making arrays on substantially continuous surfaces the compounds being directly bound or immobilised to solid supports
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00603—Making arrays on substantially continuous surfaces
    • B01J2219/00605—Making arrays on substantially continuous surfaces the compounds being directly bound or immobilised to solid supports
    • B01J2219/00612—Making arrays on substantially continuous surfaces the compounds being directly bound or immobilised to solid supports the surface being inorganic
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00603—Making arrays on substantially continuous surfaces
    • B01J2219/00605—Making arrays on substantially continuous surfaces the compounds being directly bound or immobilised to solid supports
    • B01J2219/00614—Delimitation of the attachment areas
    • B01J2219/00617—Delimitation of the attachment areas by chemical means
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00603—Making arrays on substantially continuous surfaces
    • B01J2219/00605—Making arrays on substantially continuous surfaces the compounds being directly bound or immobilised to solid supports
    • B01J2219/00614—Delimitation of the attachment areas
    • B01J2219/00617—Delimitation of the attachment areas by chemical means
    • B01J2219/00619—Delimitation of the attachment areas by chemical means using hydrophilic or hydrophobic regions
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00603—Making arrays on substantially continuous surfaces
    • B01J2219/00605—Making arrays on substantially continuous surfaces the compounds being directly bound or immobilised to solid supports
    • B01J2219/00623—Immobilisation or binding
    • B01J2219/00626—Covalent
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00603—Making arrays on substantially continuous surfaces
    • B01J2219/00605—Making arrays on substantially continuous surfaces the compounds being directly bound or immobilised to solid supports
    • B01J2219/00632—Introduction of reactive groups to the surface
    • B01J2219/00637—Introduction of reactive groups to the surface by coating it with another layer
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00603—Making arrays on substantially continuous surfaces
    • B01J2219/00659—Two-dimensional arrays
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00718—Type of compounds synthesised
    • B01J2219/0072—Organic compounds
    • B01J2219/00722—Nucleotides
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07B—GENERAL METHODS OF ORGANIC CHEMISTRY; APPARATUS THEREFOR
    • C07B2200/00—Indexing scheme relating to specific properties of organic compounds
    • C07B2200/11—Compounds covalently bound to a solid support
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B40/00—Libraries per se, e.g. arrays, mixtures
    • C40B40/04—Libraries containing only organic compounds
    • C40B40/06—Libraries containing nucleotides or polynucleotides, or derivatives thereof
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B60/00—Apparatus specially adapted for use in combinatorial chemistry or with libraries
    • C40B60/14—Apparatus specially adapted for use in combinatorial chemistry or with libraries for creating libraries

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
  • Saccharide Compounds (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
AT00914914T 1999-03-08 2000-03-07 Syntheseverfahren zum ökonomischen aufbau langer dna-sequenzen und zusammensetzungen hierfür ATE296310T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US26438899A 1999-03-08 1999-03-08
PCT/US2000/006335 WO2000053617A1 (en) 1999-03-08 2000-03-07 Methods and compositions for economically synthesizing and assembling long dna sequences

Publications (1)

Publication Number Publication Date
ATE296310T1 true ATE296310T1 (de) 2005-06-15

Family

ID=23005849

Family Applications (1)

Application Number Title Priority Date Filing Date
AT00914914T ATE296310T1 (de) 1999-03-08 2000-03-07 Syntheseverfahren zum ökonomischen aufbau langer dna-sequenzen und zusammensetzungen hierfür

Country Status (7)

Country Link
US (2) US20030186226A1 (de)
EP (1) EP1159285B1 (de)
JP (1) JP2002538790A (de)
AT (1) ATE296310T1 (de)
AU (1) AU3623800A (de)
DE (1) DE60020340T2 (de)
WO (1) WO2000053617A1 (de)

Families Citing this family (125)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6589726B1 (en) * 1991-09-04 2003-07-08 Metrigen, Inc. Method and apparatus for in situ synthesis on a solid support
EP1054726B1 (de) * 1998-02-11 2003-07-30 University of Houston, Office of Technology Transfer Vorrichtung zur durchführung chemischer und biochemischer reaktionen unter anwendung von photoerzeugten reagenzien
JP2002525590A (ja) 1998-08-28 2002-08-13 フェビット フェラリウス バイオテクノロジー ゲゼルシャフト ミット ベシュレンクテル ハフツング 分析物測定法のための支持体および該支持体を製造する方法
JP2002536977A (ja) 1999-02-19 2002-11-05 フェビット フェラリウス バイオテクノロジー ゲゼルシャフト ミット ベシュレンクテル ハフツング ポリマーの製法
JP2003519495A (ja) * 2000-01-11 2003-06-24 マキシジェン， インコーポレイテッド 多様性生成およびスクリーニングのための一体化されたシステムおよび方法
US7211654B2 (en) * 2001-03-14 2007-05-01 Regents Of The University Of Michigan Linkers and co-coupling agents for optimization of oligonucleotide synthesis and purification on solid supports
EP1392868B2 (de) † 2001-05-18 2013-09-04 Wisconsin Alumni Research Foundation Verfahren zur synthese von dna-sequenzen die photolabile linker verwenden
US10539561B1 (en) 2001-08-30 2020-01-21 Customarray, Inc. Enzyme-amplified redox microarray detection process
WO2003033718A1 (en) * 2001-10-17 2003-04-24 Global Genomics Ab Synthesis of oligonucleotides on solid support and assembly into doublestranded polynucleotides
DE60327183D1 (de) * 2002-02-28 2009-05-28 Wisconsin Alumni Res Found Verfahren zur fehlerreduzierung in nukleinsäurepopulationen
US7563600B2 (en) 2002-09-12 2009-07-21 Combimatrix Corporation Microarray synthesis and assembly of gene-length polynucleotides
US7871799B2 (en) * 2002-11-22 2011-01-18 Lawrence Livermore National Security, Llc Sequential addition of short DNA oligos in DNA-polymerase-based synthesis reactions
EP2664672A1 (de) 2003-04-17 2013-11-20 Alnylam Pharmaceuticals Inc. Modifizierte iRNA-Wirkstoffe
US7723509B2 (en) * 2003-04-17 2010-05-25 Alnylam Pharmaceuticals IRNA agents with biocleavable tethers
US8133670B2 (en) 2003-06-13 2012-03-13 Cold Spring Harbor Laboratory Method for making populations of defined nucleic acid molecules
EP1544296B8 (de) * 2003-12-18 2009-08-05 Biomethodes Verfahren zur ortspezifischen Massenmutagenese
US7314714B2 (en) * 2003-12-19 2008-01-01 Affymetrix, Inc. Method of oligonucleotide synthesis
JP2007534320A (ja) * 2004-02-27 2007-11-29 プレジデント・アンド・フェロウズ・オブ・ハーバード・カレッジ ポリヌクレオチド合成法
WO2005088310A2 (en) * 2004-03-05 2005-09-22 The Scripps Research Institute High throughput glycan microarrays
CA2571431A1 (en) * 2004-06-24 2006-01-05 The Scripps Research Institute Arrays with cleavable linkers
US20060008833A1 (en) * 2004-07-12 2006-01-12 Jacobson Joseph M Method for long, error-reduced DNA synthesis
US20070122817A1 (en) * 2005-02-28 2007-05-31 George Church Methods for assembly of high fidelity synthetic polynucleotides
CA2584984A1 (en) * 2004-10-18 2006-04-27 Codon Devices, Inc. Methods for assembly of high fidelity synthetic polynucleotides
US20060102471A1 (en) 2004-11-18 2006-05-18 Karl Maurer Electrode array device having an adsorbed porous reaction layer
WO2006068758A2 (en) * 2004-11-19 2006-06-29 The Scripps Research Institute Detection, prevention and treatment of breast cancer
US20060234264A1 (en) * 2005-03-14 2006-10-19 Affymetrix, Inc. Multiplex polynucleotide synthesis
US20070034513A1 (en) 2005-03-25 2007-02-15 Combimatrix Corporation Electrochemical deblocking solution for electrochemical oligomer synthesis on an electrode array
US9394167B2 (en) 2005-04-15 2016-07-19 Customarray, Inc. Neutralization and containment of redox species produced by circumferential electrodes
WO2007005053A1 (en) * 2005-06-30 2007-01-11 Codon Devices, Inc. Hierarchical assembly methods for genome engineering
GB0514936D0 (en) 2005-07-20 2005-08-24 Solexa Ltd Preparation of templates for nucleic acid sequencing
US20070065877A1 (en) 2005-09-19 2007-03-22 Combimatrix Corporation Microarray having a base cleavable succinate linker
US20070231805A1 (en) * 2006-03-31 2007-10-04 Baynes Brian M Nucleic acid assembly optimization using clamped mismatch binding proteins
US20070265170A1 (en) * 2006-05-15 2007-11-15 Ola Blixt Detection, prevention and treatment of ovarian cancer
WO2007136834A2 (en) * 2006-05-19 2007-11-29 Codon Devices, Inc. Combined extension and ligation for nucleic acid assembly
US8053191B2 (en) * 2006-08-31 2011-11-08 Westend Asset Clearinghouse Company, Llc Iterative nucleic acid assembly using activation of vector-encoded traits
US20090036660A1 (en) * 2007-07-31 2009-02-05 Joel Myerson Methods and compositions for generating mixtures of nucleic acid molecules
US20090075840A1 (en) * 2007-09-18 2009-03-19 Joel Myerson Methods And Compositions For Generating Mixtures Of Nucleic Acid Molecules
JP2009268665A (ja) * 2008-05-07 2009-11-19 Canon Inc 吸入装置
US8808986B2 (en) 2008-08-27 2014-08-19 Gen9, Inc. Methods and devices for high fidelity polynucleotide synthesis
WO2010091024A1 (en) * 2009-02-03 2010-08-12 Complete Genomics, Inc. Oligomer sequences mapping
EP2394164A4 (de) * 2009-02-03 2014-01-08 Complete Genomics Inc Zuordnung von oligomersequenzen
WO2010091023A2 (en) * 2009-02-03 2010-08-12 Complete Genomics, Inc. Indexing a reference sequence for oligomer sequence mapping
CN102460155B (zh) * 2009-04-29 2015-03-25 考利达基因组股份有限公司 用于关于参考多核苷酸序列标注样本多核苷酸序列中的变异的方法和系统
US10207240B2 (en) 2009-11-03 2019-02-19 Gen9, Inc. Methods and microfluidic devices for the manipulation of droplets in high fidelity polynucleotide assembly
ES2574055T3 (es) 2009-11-25 2016-06-14 Gen9, Inc. Métodos y aparatos para la reducción de errores en el ADN basada en un chip
WO2011066185A1 (en) 2009-11-25 2011-06-03 Gen9, Inc. Microfluidic devices and methods for gene synthesis
WO2011085075A2 (en) 2010-01-07 2011-07-14 Gen9, Inc. Assembly of high fidelity polynucleotides
WO2011090793A2 (en) 2010-01-20 2011-07-28 Customarray, Inc. Multiplex microarray of serially deposited biomolecules on a microarray
WO2011143556A1 (en) 2010-05-13 2011-11-17 Gen9, Inc. Methods for nucleotide sequencing and high fidelity polynucleotide synthesis
US9187777B2 (en) 2010-05-28 2015-11-17 Gen9, Inc. Methods and devices for in situ nucleic acid synthesis
GB2481425A (en) 2010-06-23 2011-12-28 Iti Scotland Ltd Method and device for assembling polynucleic acid sequences
US8865404B2 (en) 2010-11-05 2014-10-21 President And Fellows Of Harvard College Methods for sequencing nucleic acid molecules
EP3000883B8 (de) 2010-11-12 2018-02-28 Gen9, Inc. Verfahren und vorrichtungen für nukleinsäuresynthese
US10457935B2 (en) 2010-11-12 2019-10-29 Gen9, Inc. Protein arrays and methods of using and making the same
PT105960A (pt) * 2010-12-07 2012-08-16 Ass For The Advancement Of Tissue Engineering Cell Based Technologies And Therapies A4Tec Associacao Processo para deposição de biomateriais em substratos repelentes a água e resultantes biomateriais
US9752176B2 (en) 2011-06-15 2017-09-05 Ginkgo Bioworks, Inc. Methods for preparative in vitro cloning
IL280334B2 (en) 2011-08-26 2023-09-01 Gen9 Inc Preparations and methods for high-fidelity assembly of nucleic acids
US10501791B2 (en) 2011-10-14 2019-12-10 President And Fellows Of Harvard College Sequencing by structure assembly
WO2013123125A1 (en) 2012-02-17 2013-08-22 President And Fellows Of Harvard College Assembly of nucleic acid sequences in emulsions
US9150853B2 (en) 2012-03-21 2015-10-06 Gen9, Inc. Methods for screening proteins using DNA encoded chemical libraries as templates for enzyme catalysis
CN104603286B (zh) 2012-04-24 2020-07-31 Gen9股份有限公司 在体外克隆中分选核酸和多重制备物的方法
US9914967B2 (en) 2012-06-05 2018-03-13 President And Fellows Of Harvard College Spatial sequencing of nucleic acids using DNA origami probes
WO2014004393A1 (en) * 2012-06-25 2014-01-03 Gen9, Inc. Methods for nucleic acid assembly and high throughput sequencing
US9476089B2 (en) 2012-10-18 2016-10-25 President And Fellows Of Harvard College Methods of making oligonucleotide probes
TWI805996B (zh) 2013-08-05 2023-06-21 美商扭轉生物科技有限公司 重新合成之基因庫
CN105637099B (zh) 2013-08-23 2020-05-19 深圳华大智造科技有限公司 使用短读段的长片段从头组装
WO2016126987A1 (en) 2015-02-04 2016-08-11 Twist Bioscience Corporation Compositions and methods for synthetic gene assembly
WO2016126882A1 (en) 2015-02-04 2016-08-11 Twist Bioscience Corporation Methods and devices for de novo oligonucleic acid assembly
US9981239B2 (en) 2015-04-21 2018-05-29 Twist Bioscience Corporation Devices and methods for oligonucleic acid library synthesis
HK1258869A1 (zh) 2015-09-18 2019-11-22 Twist Bioscience Corporation 寡核酸变体文库及其合成
CN108698012A (zh) 2015-09-22 2018-10-23 特韦斯特生物科学公司 用于核酸合成的柔性基底
CN108603307A (zh) 2015-12-01 2018-09-28 特韦斯特生物科学公司 功能化表面及其制备
WO2018026920A1 (en) * 2016-08-03 2018-02-08 Twist Bioscience Corporation Textured surfaces for polynucleotide synthesis
WO2018038772A1 (en) 2016-08-22 2018-03-01 Twist Bioscience Corporation De novo synthesized nucleic acid libraries
EP3516528B1 (de) 2016-09-21 2025-10-15 Atlas Data Storage, Inc. Nukleinsäurebasierte datenspeicherung
WO2018112426A1 (en) 2016-12-16 2018-06-21 Twist Bioscience Corporation Variant libraries of the immunological synapse and synthesis thereof
KR20240158370A (ko) 2017-02-22 2024-11-04 트위스트 바이오사이언스 코포레이션 핵산 기반 데이터 저장
CA3056388A1 (en) 2017-03-15 2018-09-20 Twist Bioscience Corporation Variant libraries of the immunological synapse and synthesis thereof
WO2018231864A1 (en) 2017-06-12 2018-12-20 Twist Bioscience Corporation Methods for seamless nucleic acid assembly
KR102628876B1 (ko) 2017-06-12 2024-01-23 트위스트 바이오사이언스 코포레이션 심리스 핵산 어셈블리를 위한 방법
CN111566125A (zh) 2017-09-11 2020-08-21 特韦斯特生物科学公司 Gpcr结合蛋白及其合成
US10894242B2 (en) 2017-10-20 2021-01-19 Twist Bioscience Corporation Heated nanowells for polynucleotide synthesis
EP3735459B1 (de) 2018-01-04 2026-03-04 Atlas Data Storage, Inc. Digitale informationsspeicherung auf dna-basis
SG11202011467RA (en) 2018-05-18 2020-12-30 Twist Bioscience Corp Polynucleotides, reagents, and methods for nucleic acid hybridization
JP7430701B2 (ja) 2018-07-23 2024-02-13 ディーエヌエー スクリプト 核酸鎖の大規模並列酵素合成
CN113692409B (zh) 2018-12-26 2025-01-10 特韦斯特生物科学公司 高度准确的从头多核苷酸合成
SG11202106445RA (en) 2019-01-03 2021-07-29 Dna Script One pot synthesis of sets of oligonucleotides
EP3930753A4 (de) 2019-02-26 2023-03-29 Twist Bioscience Corporation Variante nukleinsäurebibliotheken für glp1-rezeptor
US11492728B2 (en) 2019-02-26 2022-11-08 Twist Bioscience Corporation Variant nucleic acid libraries for antibody optimization
CA3144644A1 (en) 2019-06-21 2020-12-24 Twist Bioscience Corporation Barcode-based nucleic acid sequence assembly
CA3145783A1 (en) 2019-07-30 2021-02-04 Tillmann HEINISCH Template-free enzymatic synthesis of polynucleotides using poly(a) and poly(u) polymerases
CN114430778B (zh) 2019-08-01 2024-07-05 Dna斯克瑞普特公司 增加多核苷酸的无模板酶促合成中长序列的产量
CN115023440B (zh) 2019-09-23 2025-09-12 特韦斯特生物科学公司 结合CD3ε的抗体
CA3151411A1 (en) 2019-09-23 2021-04-01 Adrian Horgan Increasing long-sequence yields in template-free enzymatic synthesis of polynucleotides
EP4464827A3 (de) 2019-09-23 2025-02-26 Twist Bioscience Corporation Varianten von nukleinsäurebibliotheken für crth2
US12570750B2 (en) 2019-12-09 2026-03-10 Twist Bioscience Corporation Antibodies that bind adenosine A2A receptors and methods of use thereof to treat cancer and neurological diseases
CN115151652B (zh) 2020-02-25 2025-02-07 Dna斯克瑞普特公司 用于酶促合成多核苷酸的方法和设备
EP4114936A4 (de) 2020-03-03 2024-03-27 Codex Dna, Inc. Verfahren zur montage von nukleinsäuren
JP2023522234A (ja) 2020-04-20 2023-05-29 ディーエヌエー スクリプト 末端デオキシヌクレオチジルトランスフェラーゼ変異体およびその使用
KR20230017781A (ko) 2020-04-27 2023-02-06 트위스트 바이오사이언스 코포레이션 코로나바이러스에 대한 변이체 핵산 라이브러리
EP4164784B1 (de) 2020-06-16 2025-06-25 DNA Script Verfahren zur enzymatischen polynukleotidsynthese
WO2022013094A1 (en) 2020-07-15 2022-01-20 Dna Script Massively parallel enzymatic synthesis of polynucleotides
CA3190667A1 (en) 2020-08-26 2022-03-03 Aaron Sato Methods and compositions relating to glp1r variants
US12534494B2 (en) 2020-08-28 2026-01-27 Twist Bioscience Corporation Devices and methods for synthesis
US20240093256A1 (en) 2020-09-22 2024-03-21 Dna Script Stabilized N-Terminally Truncated Terminal Deoxynucleotidyl Transferase Variants and Uses Thereof
EP4229210A4 (de) 2020-10-19 2025-01-08 Twist Bioscience Corporation Verfahren zur synthese von oligonukleotiden unter verwendung von gebundenen nukleotiden
KR20230142832A (ko) 2021-01-08 2023-10-11 셀라노메, 인크. 생물학적 샘플을 분석하기 위한 디바이스 및 방법
US12576400B2 (en) 2021-01-08 2026-03-17 Cellanome, Inc. Methods for incubating and analyzing a cell in a compartment of a fluidic device
US12202905B2 (en) 2021-01-21 2025-01-21 Twist Bioscience Corporation Methods and compositions relating to adenosine receptors
EP4314075A4 (de) 2021-03-24 2025-04-09 Twist Bioscience Corporation Varianten von nukleinsäurebibliotheken für cd3
AU2022249826A1 (en) 2021-04-02 2023-09-21 Dna Script Methods and kits for enzymatic synthesis of g4-prone polynucleotides
US12553151B2 (en) 2021-05-03 2026-02-17 Twist Bioscience Corporation Variant nucleic acid libraries for glycans
US12201857B2 (en) 2021-06-22 2025-01-21 Twist Bioscience Corporation Methods and compositions relating to covid antibody epitopes
WO2023023285A2 (en) 2021-08-19 2023-02-23 Twist Bioscience Corporation Methods and compositions relating to covalently closed nucleic acids
WO2023035003A1 (en) 2021-09-03 2023-03-09 Elegen Corp. Multi-way bead-sorting devices, systems, and methods of use thereof using pressure sources
EP4460516A2 (de) 2022-01-03 2024-11-13 Twist Bioscience Corporation Bispezifische sars-cov-2-antikörper und verfahren zur verwendung
WO2023170259A1 (en) 2022-03-11 2023-09-14 Dna Script Modular accessory rack
WO2023170258A1 (en) 2022-03-11 2023-09-14 Dna Script Apparatus for enzymatic synthesis of a plurality of polynucleotides comprising a condensation trap
WO2023170286A2 (en) 2022-03-11 2023-09-14 Dna Script Alignment post and secure mechanism for enzymatic polynucleotide synthesis
US20250205700A1 (en) 2022-03-11 2025-06-26 Dna Script Automation station for enzymatic polynucleotide synthesis
CN120603956A (zh) 2022-12-31 2025-09-05 Dna斯克瑞普特公司 用于酶试剂的喷墨递送的可变粘度油墨
WO2024153643A1 (en) 2023-01-16 2024-07-25 Dna Script Inkjet-assisted enzymatic nucleic acid synthesis
WO2025003434A1 (en) 2023-06-30 2025-01-02 Dna Script Nucleic acid synthesis on reusable support
WO2025019586A1 (en) 2023-07-18 2025-01-23 Twist Bioscience Corporation Spatially controlled data transfer
WO2025072571A1 (en) 2023-09-27 2025-04-03 Cellanome, Inc. Cell culture within microfluidic structures

Family Cites Families (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4744796A (en) * 1986-02-04 1988-05-17 Arco Chemical Company Microemulsion fuel system
US5641648A (en) * 1986-11-04 1997-06-24 Protein Polymer Technologies, Inc. Methods for preparing synthetic repetitive DNA
US5132215A (en) * 1988-09-15 1992-07-21 Eastman Kodak Company Method of making double-stranded dna sequences
US6921636B1 (en) * 1991-09-04 2005-07-26 Metrigen, Inc. Method and apparatus for conducting an array of chemical reactions on a support surface
US6589726B1 (en) * 1991-09-04 2003-07-08 Metrigen, Inc. Method and apparatus for in situ synthesis on a solid support
US5474796A (en) * 1991-09-04 1995-12-12 Protogene Laboratories, Inc. Method and apparatus for conducting an array of chemical reactions on a support surface
EP1588761A3 (de) * 1991-11-22 2005-11-23 Affymetrix, Inc. Verfahren zur Herstellung von Polymerarrays
US5834252A (en) * 1995-04-18 1998-11-10 Glaxo Group Limited End-complementary polymerase reaction
US5605793A (en) * 1994-02-17 1997-02-25 Affymax Technologies N.V. Methods for in vitro recombination
US5837458A (en) * 1994-02-17 1998-11-17 Maxygen, Inc. Methods and compositions for cellular and metabolic engineering
US6015880A (en) * 1994-03-16 2000-01-18 California Institute Of Technology Method and substrate for performing multiple sequential reactions on a matrix
US5739386A (en) * 1994-06-23 1998-04-14 Affymax Technologies N.V. Photolabile compounds and methods for their use
US6558633B1 (en) * 1994-09-21 2003-05-06 Isis Pharmaceuticals, Inc. Chemical reaction apparatus and methods
US5830655A (en) * 1995-05-22 1998-11-03 Sri International Oligonucleotide sizing using cleavable primers
US5700642A (en) * 1995-05-22 1997-12-23 Sri International Oligonucleotide sizing using immobilized cleavable primers
US5843655A (en) * 1995-09-18 1998-12-01 Affymetrix, Inc. Methods for testing oligonucleotide arrays
US6083762A (en) * 1996-05-31 2000-07-04 Packard Instruments Company Microvolume liquid handling system
DE19736591A1 (de) * 1997-08-22 1999-02-25 Peter Prof Dr Hegemann Verfahren zum Herstellen von Nukleinsäurepolymeren
US5942609A (en) * 1998-11-12 1999-08-24 The Porkin-Elmer Corporation Ligation assembly and detection of polynucleotides on solid-support

Also Published As

Publication number Publication date
US20030068643A1 (en) 2003-04-10
US20030186226A1 (en) 2003-10-02
JP2002538790A (ja) 2002-11-19
DE60020340T2 (de) 2006-03-16
WO2000053617A1 (en) 2000-09-14
EP1159285A1 (de) 2001-12-05
DE60020340D1 (de) 2005-06-30
AU3623800A (en) 2000-09-28
EP1159285B1 (de) 2005-05-25

Similar Documents

Publication Publication Date Title
ATE296310T1 (de) Syntheseverfahren zum ökonomischen aufbau langer dna-sequenzen und zusammensetzungen hierfür
DE69835357D1 (de) Verfahren zur Herstellung von Synthesegas
ATE317023T1 (de) Methode zum nachweis von variationen oder polymorphismen
ID29538A (id) SENYAWA LOGAM TRANSISI, KATALIS UNTUK PRODUKSI α-OLEFIN-OLEFIN DAN PROSES UNTUK MEMPRODUKSI α-OLEFIN-OLEFIN
DE69813792T2 (de) Zwischenprodukte und verfahren zu ihrer herstellung
DE69821347D1 (de) (2-(2-thienyl)-ethylamino)-(2-halophenyl)-acetonitrile als zwischenprodukte und verfahren zu ihrer herstellung
NO986116D0 (no) Forbedret metode for fremstilling av katalysator bestÕende av metaller av h
ATE457972T1 (de) Verfahren zur herstellung von 7-amino-syn-3,5- dihydroxyheptansäurederivaten und zwischenverbindungen diesesverfahrens und deren herestellung
ATE277915T1 (de) Verfahren sowie neue zwischenprodukte zur herstellung von isoxazolin-3-yl-acylbenzolen
EA200100213A1 (ru) Способ синтеза производных (1h)-бензо[c]хинолизин-3-онов
ATE278702T1 (de) Verfahren zur synthese von phosphorothioat- oligonukelotiden
DE60012587D1 (de) Friedel-craft verfahren zur herstellung von thioxanthonen
ATE281434T1 (de) Verfahren zur herstellung von benzoperhydroisoindolen
HUP0100943A2 (hu) Eljárás poliamidok előállítására
ATE277919T1 (de) Verfahren zur herstellung von baccatin iii- derivativen
ATE223414T1 (de) Prozess zur herstellung von zwischebprodukten für imidazodeazepinen
DE69621463D1 (de) Verfahren zum ausgewähltem schutz von baccatinderivaten und ihre verwendung für die synthese von taxanen
ATE244706T1 (de) Enantiomere von mercaptolactonen und verfahren zu ihrer herstellung
ATE191907T1 (de) Verfahren zur herstellung von zwischenprodukten, die für die herstellung von retroviral-protease hemmenden verbindungen verwendbar sind
ATE300534T1 (de) Verfahren und zwischenverbindungen zur herstellung von imidazopyridinen
DE60030674D1 (de) Verfahren zur herstellung cyclohexandimethanole und zwischenprodukte davon
NO20060816L (no) Fremgangsmate for fremstilling av a,B-umettede amidforbindelser
MX2022002533A (es) Ácidos nucleicos modificados con ligandos 2', síntesis de estos y compuestos intermedios de estos.
DE50002865D1 (de) Verfahren zur herstellung von benzofuranonoximen
PT1317427E (pt) Processo para a producao de compostos quirais

Legal Events

Date Code Title Description
RER Ceased as to paragraph 5 lit. 3 law introducing patent treaties