ATE296354T1 - Methode zur plasmidstabilisierung durch in vivo deletion der antibiotikaresistenz und selektion mit einem essentiellen gen - Google Patents

Methode zur plasmidstabilisierung durch in vivo deletion der antibiotikaresistenz und selektion mit einem essentiellen gen

Info

Publication number
ATE296354T1
ATE296354T1 AT02740737T AT02740737T ATE296354T1 AT E296354 T1 ATE296354 T1 AT E296354T1 AT 02740737 T AT02740737 T AT 02740737T AT 02740737 T AT02740737 T AT 02740737T AT E296354 T1 ATE296354 T1 AT E296354T1
Authority
AT
Austria
Prior art keywords
gene
vector
antibiotic resistance
essential
cell
Prior art date
Application number
AT02740737T
Other languages
English (en)
Inventor
Leif Isaksson
Peter Joergen Haegg
Farhad Maruf Abdulkarim
Original Assignee
Leif Isaksson
Peter Joergen Haegg
Farhad Maruf Abdulkarim
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Leif Isaksson, Peter Joergen Haegg, Farhad Maruf Abdulkarim filed Critical Leif Isaksson
Application granted granted Critical
Publication of ATE296354T1 publication Critical patent/ATE296354T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/64—General methods for preparing the vector, for introducing it into the cell or for selecting the vector-containing host
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67—General methods for enhancing the expression
    • C12N15/68—Stabilisation of the vector
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K2039/51—Medicinal preparations containing antigens or antibodies comprising whole cells, viruses or DNA/RNA
    • A61K2039/52—Bacterial cells; Fungal cells; Protozoal cells

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Cell Biology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Saccharide Compounds (AREA)
AT02740737T 2001-06-21 2002-06-19 Methode zur plasmidstabilisierung durch in vivo deletion der antibiotikaresistenz und selektion mit einem essentiellen gen ATE296354T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
SE0102204A SE0102204D0 (sv) 2001-06-21 2001-06-21 New method
PCT/EP2002/006780 WO2003000881A2 (en) 2001-06-21 2002-06-19 Method of plasmid stabilisation by in vivo deleting the antibiotic resistence and selecting with an essential gene

Publications (1)

Publication Number Publication Date
ATE296354T1 true ATE296354T1 (de) 2005-06-15

Family

ID=20284558

Family Applications (1)

Application Number Title Priority Date Filing Date
AT02740737T ATE296354T1 (de) 2001-06-21 2002-06-19 Methode zur plasmidstabilisierung durch in vivo deletion der antibiotikaresistenz und selektion mit einem essentiellen gen

Country Status (10)

Country Link
US (1) US20040234984A1 (de)
EP (1) EP1404847B1 (de)
CN (1) CN1249244C (de)
AT (1) ATE296354T1 (de)
AU (1) AU2002314183A1 (de)
DE (1) DE60204317T2 (de)
ES (1) ES2240758T3 (de)
SE (1) SE0102204D0 (de)
WO (1) WO2003000881A2 (de)
ZA (1) ZA200309767B (de)

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2868422B1 (fr) * 2004-03-31 2006-07-14 Aventis Pharma Sa Nouveaux derives pyrrolo(2,3-b) pyridine, leur preparation et leur utilisation pharmaceutique comme inhibiteurs de kinases
TWI311152B (en) * 2004-09-17 2009-06-21 Boehringer Ingelheim Rcv Gmbh & Co K Host-vector system for antibiotic-free cole1 plasmid propagation
EP2683732B1 (de) * 2011-03-11 2016-08-24 DSM IP Assets B.V. Vektor-host-system
US10987432B2 (en) * 2013-09-05 2021-04-27 The University Of Hong Kong Therapeutic delivery and expression system, methods and uses thereof
EP3083965B1 (de) * 2013-12-20 2019-07-10 Basf Se Verfahren zur produktion von bestimmten proteinen in einem mikrobiellen wirtsorganismus
ES3011213T3 (en) * 2015-12-18 2025-04-07 Glycom As Fermentative production of oligosaccharides
CA3042321A1 (en) 2016-11-01 2018-05-11 Novo Nordisk A/S Tolerogenic dna vaccine
RU2712838C1 (ru) * 2018-09-04 2020-01-31 Генетик Диагностик Энд Терапи 21 Лтд Генотерапевтический ДНК-вектор GDTT1.8NAS12, способ его получения, штамм Escherichia coli JM110-NAS, способ его получения, штамм Escherichia coli JM110-NAS/GDTT1.8NAS12, несущий генотерапевтический ДНК-вектор GDTT1.8NAS12, способ его получения, способ производства в промышленных масштабах генотерапевтического ДНК-вектора
US11279745B2 (en) 2019-04-26 2022-03-22 Novo Nordisk A/S Tolerogenic DNA vaccine
US20240271084A1 (en) 2021-05-20 2024-08-15 Gen-H Genrtic Engineering Heidelberg Gmbh Microorganism strain and method for antibiotic-free plasmid-based fermentation
JP2025507940A (ja) 2022-03-04 2025-03-21 ゲン－ハー ジェネティック エンジニアリング ハイデルベルク ゲーエムベーハー 抗生物質を含まないプラスミドベースの発酵のための微生物株及びその生成方法

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0972838B1 (de) * 1998-07-15 2004-09-15 Roche Diagnostics GmbH Escherichia coli Wirt/Vektorsystem basierend auf einer antibiotikafreien Selektion durch Komplementation einer Auxotrophie
US6291245B1 (en) * 1998-07-15 2001-09-18 Roche Diagnostics Gmbh Host-vector system
FR2784394B1 (fr) * 1998-10-09 2001-01-05 Lesaffre & Cie Cassette d'integration-excision

Also Published As

Publication number Publication date
SE0102204D0 (sv) 2001-06-21
EP1404847B1 (de) 2005-05-25
AU2002314183A1 (en) 2003-01-08
DE60204317D1 (de) 2005-06-30
WO2003000881A3 (en) 2003-05-01
US20040234984A1 (en) 2004-11-25
CN1518599A (zh) 2004-08-04
EP1404847A2 (de) 2004-04-07
DE60204317T2 (de) 2006-01-26
ES2240758T3 (es) 2005-10-16
ZA200309767B (en) 2005-07-27
WO2003000881A2 (en) 2003-01-03
CN1249244C (zh) 2006-04-05

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