ATE297980T1 - Verfahren zur klonierung von schweinen - Google Patents

Verfahren zur klonierung von schweinen

Info

Publication number
ATE297980T1
ATE297980T1 AT99958900T AT99958900T ATE297980T1 AT E297980 T1 ATE297980 T1 AT E297980T1 AT 99958900 T AT99958900 T AT 99958900T AT 99958900 T AT99958900 T AT 99958900T AT E297980 T1 ATE297980 T1 AT E297980T1
Authority
AT
Austria
Prior art keywords
cloning
cells
porcine
relates
porcine animals
Prior art date
Application number
AT99958900T
Other languages
English (en)
Inventor
Philip Damiani
Jeffrey M Betthauser
Erik J Forsberg
Michael D Bishop
Original Assignee
Infigen Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Infigen Inc filed Critical Infigen Inc
Application granted granted Critical
Publication of ATE297980T1 publication Critical patent/ATE297980T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/873—Techniques for producing new embryos, e.g. nuclear transfer, manipulation of totipotent cells or production of chimeric embryos
    • C12N15/877—Techniques for producing new mammalian cloned embryos
    • C12N15/8778—Swine embryos
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0603—Embryonic cells ; Embryoid bodies
    • C12N5/0606—Pluripotent embryonic cells, e.g. embryonic stem cells [ES]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0608—Germ cells
    • C12N5/0611—Primordial germ cells, e.g. embryonic germ cells [EG]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/115—Basic fibroblast growth factor (bFGF, FGF-2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/20—Cytokines; Chemokines
    • C12N2501/23—Interleukins [IL]
    • C12N2501/235—Leukemia inhibitory factor [LIF]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2502/00—Coculture with; Conditioned medium produced by
    • C12N2502/13—Coculture with; Conditioned medium produced by connective tissue cells; generic mesenchyme cells, e.g. so-called "embryonic fibroblasts"

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Developmental Biology & Embryology (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Reproductive Health (AREA)
  • Gynecology & Obstetrics (AREA)
  • Cell Biology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Fodder In General (AREA)
  • Feed For Specific Animals (AREA)
  • Peptides Or Proteins (AREA)
AT99958900T 1998-11-24 1999-11-12 Verfahren zur klonierung von schweinen ATE297980T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US09/199,138 US6258998B1 (en) 1998-11-24 1998-11-24 Method of cloning porcine animals
PCT/US1999/026710 WO2000031237A2 (en) 1998-11-24 1999-11-12 Method of cloning porcine animals

Publications (1)

Publication Number Publication Date
ATE297980T1 true ATE297980T1 (de) 2005-07-15

Family

ID=22736378

Family Applications (1)

Application Number Title Priority Date Filing Date
AT99958900T ATE297980T1 (de) 1998-11-24 1999-11-12 Verfahren zur klonierung von schweinen

Country Status (12)

Country Link
US (1) US6258998B1 (de)
EP (2) EP1586633A1 (de)
JP (1) JP2002530105A (de)
AT (1) ATE297980T1 (de)
AU (1) AU774430B2 (de)
BR (1) BR9915642A (de)
CA (1) CA2350831A1 (de)
DE (1) DE69925856T2 (de)
IL (1) IL143326A0 (de)
NZ (1) NZ530018A (de)
WO (1) WO2000031237A2 (de)
ZA (1) ZA200104230B (de)

Families Citing this family (54)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2000022098A1 (en) 1998-10-12 2000-04-20 Geron Bio-Med Limited Porcine oocytes with improved developmental competence
US6700037B2 (en) * 1998-11-24 2004-03-02 Infigen, Inc. Method of cloning porcine animals
US20040180430A1 (en) * 1999-09-07 2004-09-16 West Michael D. Methods of restoring telomere length and extending cell lifespan using nuclear transfer
US20050255596A1 (en) * 1999-09-07 2005-11-17 West Michael D Methods of repairing tandemly repeated DNA sequences and extending cell life-span nuclear transfer
US7820878B2 (en) * 1999-11-19 2010-10-26 Kyowa Hakko Kirin Co., Ltd. Production of ungulates, preferably bovines that produce human immunoglobulins
US7074983B2 (en) * 1999-11-19 2006-07-11 Kirin Beer Kabushiki Kaisha Transgenic bovine comprising human immunoglobulin loci and producing human immunoglobulin
US7253334B2 (en) * 2000-12-22 2007-08-07 Aurox, Llc Methods for cloning non-human mammals using reprogrammed donor chromatin or donor cells
US7414170B2 (en) * 1999-11-19 2008-08-19 Kirin Beer Kabushiki Kaisha Transgenic bovines capable of human antibody production
AU1777301A (en) * 1999-11-19 2001-05-30 Hematech, Llc Production of ungulates, preferably bovines that produce human immunoglobulins
US20060174358A1 (en) * 2001-01-04 2006-08-03 Xiangzhong Yang Method for cloning animals with targetted genetic alterations by transfer of long-term cultured male or female somatic cell nuclei, comprising artificially-induced genetic alterations, to enucleated recipient cells
CN1293188C (zh) * 2000-01-04 2007-01-03 康涅狄格州大学 一种将长期培养的可包括人工诱导基因改变的雌或雄体细胞的细胞核转移到去核受体细胞中实现靶向基因改变的克隆动物的方法
US7491534B2 (en) * 2000-12-22 2009-02-17 Kirin Holdings Kabushiki Kaisha Methods for altering cell fate to generate T-cells specific for an antigen of interest
US20020142397A1 (en) * 2000-12-22 2002-10-03 Philippe Collas Methods for altering cell fate
AUPR247901A0 (en) * 2001-01-10 2001-02-01 Garelag Pty Ltd Activation of nuclear transfer embryos
CA2435857A1 (en) 2001-01-30 2002-09-26 Rebholtz, Amy K. Methods for embryo recipient management
WO2002062131A2 (en) * 2001-02-02 2002-08-15 Infigen, Inc. Method of cloning transgenic mammalian animals using pseudonuclei
US7265262B2 (en) 2001-03-21 2007-09-04 Roslin Institute (Edinburgh) Telomerizing nuclear donor cells and improving the efficiency on nuclear transfer
AUPR446701A0 (en) 2001-04-18 2001-05-17 Gene Stream Pty Ltd Transgenic mammals for pharmacological and toxicological studies
AU2002326353A1 (en) * 2001-07-09 2003-01-29 Alexion Pharmaceuticals Inc. Cloned non-human mammals from contact inhibited donor cells
US20030170678A1 (en) * 2001-10-25 2003-09-11 Neurogenetics, Inc. Genetic markers for Alzheimer's disease and methods using the same
US20030224380A1 (en) * 2001-10-25 2003-12-04 The General Hospital Corporation Genes and polymorphisms on chromosome 10 associated with Alzheimer's disease and other neurodegenerative diseases
AU2002364945A1 (en) * 2001-10-25 2003-07-09 Neurogenetics, Inc. Genes and polymorphisms on chromosome 10 associated with alzheimer's disease and other neurodegenerative diseases
CA2471035C (en) * 2001-12-21 2014-05-13 The Curators Of The University Of Missouri Knockout swine and methods for making the same
AU2002217606A1 (en) * 2001-12-29 2003-11-03 Woo Suk Hwang Gfp-transfected clon pig, gt knock-out clon pig and methods for production thereof
JP2003225089A (ja) * 2002-02-04 2003-08-12 National Institute Of Agrobiological Sciences ブタrag−1遺伝子およびその利用
US7736892B2 (en) 2002-02-25 2010-06-15 Kansas State University Research Foundation Cultures, products and methods using umbilical cord matrix cells
US20030161818A1 (en) * 2002-02-25 2003-08-28 Kansas State University Research Foundation Cultures, products and methods using stem cells
US20030217378A1 (en) * 2002-04-05 2003-11-20 The University Of Georgia Research Foundation, Inc Cloning using rapidly matured oocytes
US7371922B2 (en) * 2002-07-31 2008-05-13 The Board Of Trustees Of The University Of Illinois Nuclear transfer with porcine embryonic stem cells
WO2004013299A2 (en) * 2002-08-02 2004-02-12 The General Hospital Corporation Methods for the production of cells and mammals with desired genetic modifications
WO2004013606A2 (en) 2002-08-02 2004-02-12 Stratatech Corporation Species specific dna detection
ES2338111T3 (es) 2002-08-21 2010-05-04 Revivicor, Inc. Animales porcinos que carecen de cualquier expresion de alfa 1,3 galactosiltransferasa funcional.
US20040077077A1 (en) * 2002-10-18 2004-04-22 Xiangzhong Yang Novel methods for the production of cloned mammals, mammals cloned according to the methods, and methods of use of same
JP2006505291A (ja) * 2002-11-08 2006-02-16 ヘマテック,エルエルシー プリオンタンパク質活性が低減されたトランスジェニック有蹄動物及びその用途
US7560538B2 (en) * 2003-11-05 2009-07-14 University Of Pittsburgh Porcine isogloboside 3 synthase protein, cDNA, genomic organization, and regulatory region
DK1734814T3 (en) 2004-03-17 2015-04-07 Revivicor Inc Tissue DERIVED FROM ANIMALS IS MISSING ANY EXPRESSION OF FUNCTIONAL ALPHA-1,3 galactosyltransferase
CA2563064A1 (en) 2004-04-22 2005-11-10 Kirin Beer Kabushiki Kaisha Transgenic animals and uses thereof
JP2006081542A (ja) * 2004-08-18 2006-03-30 Institute Of Physical & Chemical Research クローン哺乳動物の作成方法
WO2006036975A2 (en) * 2004-09-28 2006-04-06 Viagen, Inc. Methods of embryo transfer
US20060148080A1 (en) * 2004-12-30 2006-07-06 Paul Diamond Methods for supporting and producing human cells and tissues in non-human mammal hosts
US20060147429A1 (en) * 2004-12-30 2006-07-06 Paul Diamond Facilitated cellular reconstitution of organs and tissues
US20090186097A1 (en) 2005-08-09 2009-07-23 David Lee Ayares Transgenic Ungulates Expressing CTLA4-IG and Uses Thereof
KR100807644B1 (ko) 2007-03-19 2008-02-28 충남대학교산학협력단 체외수정 수정란 및 체세포 복제 수정란의 동시 이식에의한 돼지 생산 방법
US9420770B2 (en) 2009-12-01 2016-08-23 Indiana University Research & Technology Corporation Methods of modulating thrombocytopenia and modified transgenic pigs
CA2827348C (en) 2011-02-14 2021-02-23 Revivicor, Inc. Genetically modified pigs for xenotransplantation of vascularized xenografts and derivatives thereof
US9781919B2 (en) 2011-06-01 2017-10-10 Inguran, Llc Compositions and methods for improving the quality of processed sperm
BR112013030815B1 (pt) 2011-06-01 2019-02-19 Inguran, Llc Composições e método para aprimorar a qualidade do esperma processado
US20140115728A1 (en) 2012-10-24 2014-04-24 A. Joseph Tector Double knockout (gt/cmah-ko) pigs, organs and tissues
EP2993234B1 (de) 2013-04-30 2018-10-10 Konkuk University Industrial Cooperation Corp. Cmp-acetylneuraminsäure-hydroxylase-targeting-vektor, transgenes tier zur mit dem vektor eingeführten xenotransplantation und verfahren zur herstellung davon
GB201314452D0 (en) * 2013-08-13 2013-09-25 Ostara Biomedical Ltd Embryo implantation
GB201501302D0 (en) 2015-01-27 2015-03-11 Ostara Biomedical Ltd Embryo implantation
GB201517523D0 (en) 2015-10-05 2015-11-18 Ostara Biomedical Ltd Methods and compositions for managing reproduction
CN117279502A (zh) 2020-11-20 2023-12-22 雷维维科公司 用于异种移植的生长激素受体敲除的多转基因猪
EP4404740A1 (de) 2021-09-20 2024-07-31 Revivicor Inc. Multitransgene schweine mit zehn genetischen modifikationen zur xenotransplantation

Family Cites Families (34)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4664097A (en) 1984-05-09 1987-05-12 The Wistar Institute Of Anatomy & Biology Nuclear transplantation in the mammalian embryo by microsurgery and cell fusion
ATE141646T1 (de) 1986-04-09 1996-09-15 Genzyme Corp Genetisch transformierte tiere, die ein gewünschtes protein in milch absondern
US4987071A (en) 1986-12-03 1991-01-22 University Patents, Inc. RNA ribozyme polymerases, dephosphorylases, restriction endoribonucleases and methods
US4994384A (en) 1986-12-31 1991-02-19 W. R. Grace & Co.-Conn. Multiplying bovine embryos
US5346990A (en) 1987-04-08 1994-09-13 Cytogam, Inc. Sex-associated membrane proteins and methods for increasing the probability that offspring will be of a desired sex
US5021244A (en) 1988-12-06 1991-06-04 Cytogam, Inc. Sex-associated membrane antibodies and their use for increasing the probability that offspring will be of a desired sex
US5057420A (en) 1987-06-05 1991-10-15 Granada Biosciences, Inc. Bovine nuclear transplantation
US5213979A (en) 1987-12-30 1993-05-25 W. R. Grace & Co.-Conn. In vitro culture of bovine embryos
US5633076A (en) 1989-12-01 1997-05-27 Pharming Bv Method of producing a transgenic bovine or transgenic bovine embryo
US5160312A (en) 1990-02-09 1992-11-03 W. R. Grace & Co.-Conn. Cryopreservation process for direct transfer of embryos
US5096822A (en) 1990-07-26 1992-03-17 W. R. Grace & Co.- Conn. Bovine embryo medium
DE69133557D1 (de) 1990-08-29 2007-03-15 Pharming Intellectual Pty Bv Homologe rekombination in säugetier-zellen
AU3779693A (en) 1992-02-28 1993-09-13 Biotransplant Incorporated Antibodies for and treatment to prevent or reduce the severity of xenograft rejection
WO1993022432A1 (en) 1992-05-01 1993-11-11 Genzyme Corporation Method for identifying transgenic preimplantation embryos
US5645986A (en) 1992-05-13 1997-07-08 Board Of Reagents, The University Of Texas System Therapy and diagnosis of conditions related to telomere length and/or telomerase activity
US5453357A (en) 1992-10-08 1995-09-26 Vanderbilt University Pluripotential embryonic stem cells and methods of making same
US5589582A (en) 1992-10-27 1996-12-31 Biotransplant, Inc. Polynucleotides en coding porcine cytokines
US5496720A (en) 1993-02-10 1996-03-05 Susko-Parrish; Joan L. Parthenogenic oocyte activation
ES2146648T3 (es) 1993-03-09 2000-08-16 Genzyme Corp Procedimiento de aislamiento de proteinas de la leche.
US5523226A (en) * 1993-05-14 1996-06-04 Biotechnology Research And Development Corp. Transgenic swine compositions and methods
WO1995010599A1 (en) 1993-10-15 1995-04-20 The University Of Melbourne Embryonic stem cell-like cells
US5827690A (en) 1993-12-20 1998-10-27 Genzyme Transgenics Corporatiion Transgenic production of antibodies in milk
GB9401380D0 (en) 1994-01-25 1994-03-23 Pharmaceutical Proteins Ltd Embryonic cell isolation
JPH10504442A (ja) 1994-04-13 1998-05-06 バイオトランスプラント,インコーポレイテッド α(1,3)ガラクトシルトランスフェラーゼ陰性ブタ
WO1996006165A1 (en) 1994-08-19 1996-02-29 The General Hospital Corporation Genetically engineered swine cells
GB9417831D0 (en) 1994-09-05 1994-10-26 Biotech & Biolog Scien Res Biological manipulation
GB9517780D0 (en) 1995-08-31 1995-11-01 Roslin Inst Edinburgh Biological manipulation
GB9517779D0 (en) 1995-08-31 1995-11-01 Roslin Inst Edinburgh Biological manipulation
US6166288A (en) 1995-09-27 2000-12-26 Nextran Inc. Method of producing transgenic animals for xenotransplantation expressing both an enzyme masking or reducing the level of the gal epitope and a complement inhibitor
WO1997020035A1 (en) * 1995-11-29 1997-06-05 Utah State University Establishment, maintenance, and transfection of totipotent embryonic stem cells from the embryos of domestic animals
US5905042A (en) 1996-04-01 1999-05-18 University Of Massachusetts, A Public Institution Of Higher Education Of The Commonwealth Of Massachusetts, As Represented By Its Amherst Campus Cultured inner cell mass cell lines derived from bovine or porcine embryos
WO1998016630A1 (en) 1996-10-11 1998-04-23 The Texas A & M University System Methods for the generation of primordial germ cells and transgenic animal species
JP2002512510A (ja) * 1997-03-06 2002-04-23 インフィジェン・インコーポレーテッド 動物のクローニング方法
WO1999009141A1 (en) * 1997-08-14 1999-02-25 Biotransplant, Inc. Porcine totipotent cells and method for long-term culture

Also Published As

Publication number Publication date
BR9915642A (pt) 2001-08-07
IL143326A0 (en) 2002-04-21
EP1131409B1 (de) 2005-06-15
EP1586633A1 (de) 2005-10-19
WO2000031237A3 (en) 2000-11-16
EP1131409A2 (de) 2001-09-12
AU1617500A (en) 2000-06-13
WO2000031237A2 (en) 2000-06-02
ZA200104230B (en) 2002-08-23
CA2350831A1 (en) 2000-06-02
JP2002530105A (ja) 2002-09-17
DE69925856T2 (de) 2006-05-11
AU774430B2 (en) 2004-06-24
US6258998B1 (en) 2001-07-10
DE69925856D1 (de) 2005-07-21
NZ530018A (en) 2005-08-26

Similar Documents

Publication Publication Date Title
DE69925856D1 (de) Verfahren zur klonierung von schweinen
WO2002010337A3 (en) Method of cloning porcine animals
BR9808192A (pt) Processo de clonagem de animais
MEP44408A (en) Anti-il-12 antibodies, compositions, methods and uses
DK0713495T3 (da) Fremgangsmåder til forstærkning af den biologiske aktivitet af chemokiner
DE68919856D1 (de) Nachrichtenvermittlungselement zur übertragung von zellen unterteilt in teilzellen.
DE3882822D1 (de) Verfahren zur betriebsmittellokalisierung in rechnernetzen.
DK1133558T3 (da) Sammensætninger og fremgangsmåder til forögelse af knoglemineralisering
ATE536372T1 (de) Verfahren zur hoher expression der aktiven form von lymphotoxin-beta-rezeptor-immunglobulin fusionsproteinen, und deren reinigung
ATE279441T1 (de) Verfahren zur herstellung von genetisch veränderten lymphozyten vorläuferzellen von wirbeltieren und deren gebrauch zur produktion von heterologen bindeproteinen
DE602004003605D1 (de) Auf einem System basierende, automatische Vorrichtung zum Trainieren von Tieren
DE59504922D1 (de) Verfahren zur herstellung von 2-hydroxy-4-methylthiobuttersäure (mha) und deren verwendung als tierfuttermittelsupplement
DE59903972D1 (de) Verfahren zur hydrierung von aliphatischen alpha, omega-dinitrilen
DE59912242D1 (de) Verfahren zur herstellung von (s,s)-benzyl-2,8-diazabicyclo(4.3.0)nonan
ES2175095T3 (es) Marcadores de adn respecto al tamaño de una camada de cerdos.
FR2756398B1 (fr) Procede de codage avec information de region
DE59713035D1 (de) Verfahren zur Regelung der Leistungsverstärkung
DE60105175D1 (de) Verfahren und vorrichtung zur eröffnung von eiern
DE69926059D1 (de) Verfahren zur herstellung von replikas unter bewahrung des originals
DE3689276D1 (de) Verfahren zur Aktualisierung der Informationen in einem Adressenübersetzungspufferspeicher.
ATE364693T1 (de) Verfahren zur herstellung von nicht-humanen geklonten und transgenen säugetieren
DE50111689D1 (de) Verfahren zur übertragung von zusätzlichen optischen signalen im s-band
ATE341618T1 (de) Verfahren zur stabilisierung von alaninaminotransferase und die entsprechenden zusammensetzungen
ATE212969T1 (de) Verfahren zur herstellung von 2-halogenindan-1- onen
ATE407555T1 (de) Efficienter kerntransfer mit primordialen keimzellen

Legal Events

Date Code Title Description
RER Ceased as to paragraph 5 lit. 3 law introducing patent treaties