ATE309260T1 - Elektrophoretische reinigungsmethode von nukleinsäuren - Google Patents

Elektrophoretische reinigungsmethode von nukleinsäuren

Info

Publication number
ATE309260T1
ATE309260T1 AT99902378T AT99902378T ATE309260T1 AT E309260 T1 ATE309260 T1 AT E309260T1 AT 99902378 T AT99902378 T AT 99902378T AT 99902378 T AT99902378 T AT 99902378T AT E309260 T1 ATE309260 T1 AT E309260T1
Authority
AT
Austria
Prior art keywords
nucleic acid
nucleic acids
purification method
electrophoretic purification
concentration
Prior art date
Application number
AT99902378T
Other languages
English (en)
Inventor
Gary Slater
J William Efcavitch
Guy Drouin
Pascal Mayer
Jean Rousseau
Hong Yan Zhou
Claudia Chiesa
Robert Ruhfel
Roger O'neill
Original Assignee
Applera Corp
Univ Ottawa
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Applera Corp, Univ Ottawa filed Critical Applera Corp
Application granted granted Critical
Publication of ATE309260T1 publication Critical patent/ATE309260T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • C12N15/101—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by chromatography, e.g. electrophoresis, ion-exchange, reverse phase
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Analytical Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Wood Science & Technology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Immunology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Electrochemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Information Retrieval, Db Structures And Fs Structures Therefor (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Electrostatic Separation (AREA)
AT99902378T 1998-01-30 1999-01-19 Elektrophoretische reinigungsmethode von nukleinsäuren ATE309260T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US09/016,531 US6146511A (en) 1998-01-30 1998-01-30 Electrophoretic nucleic acid purification method
PCT/US1999/001137 WO1999038874A2 (en) 1998-01-30 1999-01-19 Electrophoretic nucleic acid purification method

Publications (1)

Publication Number Publication Date
ATE309260T1 true ATE309260T1 (de) 2005-11-15

Family

ID=21777601

Family Applications (1)

Application Number Title Priority Date Filing Date
AT99902378T ATE309260T1 (de) 1998-01-30 1999-01-19 Elektrophoretische reinigungsmethode von nukleinsäuren

Country Status (8)

Country Link
US (2) US6146511A (de)
EP (1) EP1056758B1 (de)
JP (1) JP2002502020A (de)
AT (1) ATE309260T1 (de)
AU (1) AU734170B2 (de)
CA (1) CA2318973A1 (de)
DE (1) DE69928230T2 (de)
WO (1) WO1999038874A2 (de)

Families Citing this family (37)

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US6146511A (en) 1998-01-30 2000-11-14 The Perkin-Elmer Corporation Electrophoretic nucleic acid purification method
US6511831B1 (en) * 1998-05-04 2003-01-28 Fraunhofer-Gesellschaft Zur Forderung Der Angewandten Forschung E.V. Electrical integrated nucleic acid isolation, purification and detection
US20020003089A1 (en) * 2000-05-23 2002-01-10 Devault James D. Electrophoretic stacking of DNA and other samples prior to electrophoresis
IL157813A0 (en) * 2001-03-08 2004-03-28 Ethrog Biotechnology Ltd Apparatus and method for electrophoresis
US7503904B2 (en) * 2002-04-25 2009-03-17 Cardiac Pacemakers, Inc. Dual balloon telescoping guiding catheter
JP4554365B2 (ja) * 2002-09-11 2010-09-29 テンプル・ユニバーシティ−オブ・ザ・コモンウェルス・システム・オブ・ハイアー・エデュケイション 高処理能力電気泳動分離のための自動化システム
DE60324458D1 (de) * 2002-11-28 2008-12-11 Arkray Inc Verfahren und apparat zum anreichern und zur aufreinigung von nukleinsäure
CN100491391C (zh) * 2002-11-28 2009-05-27 爱科来株式会社 浓缩和纯化核酸的方法和装置
US7601491B2 (en) 2003-02-06 2009-10-13 Becton, Dickinson And Company Pretreatment method for extraction of nucleic acid from biological samples and kits therefor
JP2005110503A (ja) * 2003-10-02 2005-04-28 Arkray Inc 核酸の精製方法及びデバイス
WO2005045023A1 (ja) 2003-11-10 2005-05-19 Arkray Inc. 核酸の濃縮精製方法および装置
US8518228B2 (en) 2011-05-20 2013-08-27 The University Of British Columbia Systems and methods for enhanced SCODA
US8529744B2 (en) 2004-02-02 2013-09-10 Boreal Genomics Corp. Enrichment of nucleic acid targets
US9186685B2 (en) 2012-01-13 2015-11-17 The University Of British Columbia Multiple arm apparatus and methods for separation of particles
EP1720636A4 (de) * 2004-02-02 2012-06-20 Univ British Columbia Scodaphorese und verfahren und vorrichtungen zum bewegen und aufkonzentrieren von teilchen
US10337054B2 (en) 2004-02-02 2019-07-02 Quantum-Si Incorporated Enrichment of nucleic acid targets
CA2496294A1 (en) * 2005-02-07 2006-08-07 The University Of British Columbia Apparatus and methods for concentrating and separating particles such as molecules
EP1931979B1 (de) 2005-10-04 2015-11-18 Headway Technologies, Inc. Mikrofluidischer nachweis von analyten
JP4195039B2 (ja) * 2006-02-16 2008-12-10 善之 辻本 検体の前処理方法および試薬
KR100661482B1 (ko) * 2006-04-03 2006-12-27 전남대학교산학협력단 Dna 정제를 위한 수집홈을 갖는 전기영동장치
US20080047835A1 (en) * 2006-08-22 2008-02-28 Macconnell William P Genomic DNA Purifier
EP2062036A4 (de) * 2006-08-31 2016-09-21 Life Technologies Israel Ltd Verfahren, kassetten, gele und vorrichtungen zur isolation und sammlung von biomolekülen aus elektrophorese-gelen
CA2713313A1 (en) * 2008-02-01 2009-08-06 The University Of British Columbia Methods and apparatus for particle introduction and recovery
CA2717308A1 (en) * 2008-03-03 2009-09-17 Oregon Health & Science University Method for the purification of biological macromolecules
WO2010121381A1 (en) 2009-04-21 2010-10-28 The University Of British Columbia System and methods for detection of particles
SG178581A1 (en) * 2009-09-01 2012-04-27 Univ Oregon Health & Science Reversible current gel electrophoresis device for separating biological macromolecules
JP5246128B2 (ja) * 2009-10-09 2013-07-24 凸版印刷株式会社 電気泳動用ゲルカセット製造方法及び電気泳動用ゲルの充填方法
JP6285859B2 (ja) * 2011-06-16 2018-02-28 ブリティッシュ コロンビア キャンサー エイジェンシー ブランチＢｒｉｔｉｓｈ Ｃｏｌｕｍｂｉａ Ｃａｎｃｅｒ Ａｇｅｎｃｙ Ｂｒａｎｃｈ 核酸の自動サイズ選択
WO2013166444A2 (en) 2012-05-04 2013-11-07 Boreal Genomics Corp. Biomarker analysis using scodaphoresis
US9340835B2 (en) 2013-03-15 2016-05-17 Boreal Genomics Corp. Method for separating homoduplexed and heteroduplexed nucleic acids
WO2016185284A1 (en) 2015-05-20 2016-11-24 Boreal Genomics, Inc. Method for isolating target nucleic acid using heteroduplex binding proteins
US11207679B2 (en) 2018-04-13 2021-12-28 Regents Of The University Of Minnesota DNA extraction device
JP6905961B2 (ja) * 2018-07-12 2021-07-21 株式会社日立ハイテク 電気泳動方法、電気泳動システム及び電気泳動ゲル
EP4051903A1 (de) 2019-10-29 2022-09-07 Quantum-Si Incorporated Peristaltisches pumpen von fluiden und zugehörige verfahren, systeme und vorrichtungen
WO2022064587A1 (ja) * 2020-09-24 2022-03-31 株式会社日立ハイテク 電気泳動回収装置、及び核酸前処理装置
CN114277027A (zh) * 2021-12-23 2022-04-05 深圳大学 一种用于电泳分离物回收的用具及其使用方法
CN115228262B (zh) * 2022-08-22 2023-12-01 湖南国测生物科技有限公司 实验室密闭空间内核酸污染的立体净化方法

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Publication number Priority date Publication date Assignee Title
JPS6113148A (ja) * 1984-06-29 1986-01-21 Hitachi Ltd 連続型核酸断片電気泳動装置
DE3428385A1 (de) * 1984-08-01 1986-02-20 Detlev Prof.Dr. 4000 Düsseldorf Riesner Elektrophoretisches verfahren zur trennung von biopolymeren
CA2012379C (en) * 1989-04-24 2000-01-25 Gary W. Slater Processes for the preparation and separation of macromolecules
US5284940A (en) * 1990-11-14 1994-02-08 Hri Research, Inc. Preparation for nucleic acid samples
US5217593A (en) * 1991-03-13 1993-06-08 Macconnell William P Nucleic acid purification system and method
US5139637A (en) * 1991-03-13 1992-08-18 Macconnell William P Plasmid purification system and method
US5209831A (en) * 1991-06-14 1993-05-11 Macconnell William P Bufferless electrophoresis system and method
US5178737A (en) * 1991-12-04 1993-01-12 The University Of North Carolina At Chapel Hill Electrophoretic resolution of single strand DNA by asymmetric field inversion
EP0590327B1 (de) * 1992-09-11 2003-04-09 F. Hoffmann-La Roche Ag Nachweis von Nukleinsäuren im Blut
US5482836A (en) * 1993-01-14 1996-01-09 The Regents Of The University Of California DNA purification by triplex-affinity capture and affinity capture electrophoresis
DE69427690T2 (de) * 1993-04-07 2002-05-02 Gradipore Ltd., Frenchs Forest Verbesserungen im auftrennen von proteinen
US5384022A (en) * 1993-09-08 1995-01-24 Cornell Research Foundation, Inc. Method and apparatus for electrophoretic DNA band isolation
US5453162A (en) * 1993-09-09 1995-09-26 University Of North Carolina At Chapel Hill Method and apparatus for gel electrophoresis using two electric fields
US5891313A (en) * 1993-11-23 1999-04-06 The Perkin-Elmer Corp. Entrapment of nucleic acid sequencing template in sample mixtures by entangled polymer networks
WO1995014921A1 (en) * 1993-11-23 1995-06-01 Perkin-Elmer Corporation Entrapment of nucleic acid sequencing template in sample mixtures by entangled polymer networks
US5538614A (en) * 1994-08-17 1996-07-23 Han; Dawn D. Macromolecule recovery cassette
US5587062A (en) * 1996-01-24 1996-12-24 Shimadzu Corporation Sample collecting apparatus by gel electrophoresis
US6120985A (en) * 1997-10-31 2000-09-19 Bbi Bioseq, Inc. Pressure-enhanced extraction and purification
US6146511A (en) 1998-01-30 2000-11-14 The Perkin-Elmer Corporation Electrophoretic nucleic acid purification method

Also Published As

Publication number Publication date
DE69928230D1 (de) 2005-12-15
WO1999038874A2 (en) 1999-08-05
US6827830B1 (en) 2004-12-07
CA2318973A1 (en) 1999-08-05
EP1056758A2 (de) 2000-12-06
AU734170B2 (en) 2001-06-07
EP1056758B1 (de) 2005-11-09
DE69928230T2 (de) 2006-07-20
WO1999038874A3 (en) 1999-09-23
AU2237499A (en) 1999-08-16
US6146511A (en) 2000-11-14
JP2002502020A (ja) 2002-01-22

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