ATE309387T1 - Methode zur vervielfältigung und zum nachweis eines gewünschten nukleinsäurefragments - Google Patents

Methode zur vervielfältigung und zum nachweis eines gewünschten nukleinsäurefragments

Info

Publication number
ATE309387T1
ATE309387T1 AT97914790T AT97914790T ATE309387T1 AT E309387 T1 ATE309387 T1 AT E309387T1 AT 97914790 T AT97914790 T AT 97914790T AT 97914790 T AT97914790 T AT 97914790T AT E309387 T1 ATE309387 T1 AT E309387T1
Authority
AT
Austria
Prior art keywords
probe
nucleic acid
detection
analyte
duplication
Prior art date
Application number
AT97914790T
Other languages
English (en)
Inventor
Richard C Ebersole
Edwin R Hendrickson
Sandra Fitzpatrick-Mcelligott
Michael P Perry
Original Assignee
Du Pont
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Du Pont filed Critical Du Pont
Application granted granted Critical
Publication of ATE309387T1 publication Critical patent/ATE309387T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
AT97914790T 1996-03-01 1997-02-27 Methode zur vervielfältigung und zum nachweis eines gewünschten nukleinsäurefragments ATE309387T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US1263696P 1996-03-01 1996-03-01
PCT/US1997/002892 WO1997032044A1 (en) 1996-03-01 1997-02-27 A method for the amplification and detection of a nucleic acid fragment of interest

Publications (1)

Publication Number Publication Date
ATE309387T1 true ATE309387T1 (de) 2005-11-15

Family

ID=21755944

Family Applications (1)

Application Number Title Priority Date Filing Date
AT97914790T ATE309387T1 (de) 1996-03-01 1997-02-27 Methode zur vervielfältigung und zum nachweis eines gewünschten nukleinsäurefragments

Country Status (6)

Country Link
US (1) US20030087271A1 (de)
EP (1) EP0892856B1 (de)
AT (1) ATE309387T1 (de)
CA (1) CA2246238A1 (de)
DE (1) DE69734576T2 (de)
WO (1) WO1997032044A1 (de)

Families Citing this family (23)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20030040114A1 (en) * 1996-09-05 2003-02-27 Warner-Lambert Company, Small molecule modulation of ribozymes
US7803528B1 (en) 1997-02-28 2010-09-28 Quest Diagnostics Incorporated Fluorescence energy transfer by competitive hybridization
GB9807045D0 (en) * 1998-04-01 1998-06-03 Rudi Knut Nucleic acid detection method
AU1259801A (en) * 1999-10-27 2001-05-08 Universite De Liege Detection method by pcr
US7306904B2 (en) * 2000-02-18 2007-12-11 Olink Ab Methods and kits for proximity probing
CN1348096A (zh) 2000-10-10 2002-05-08 栾国彦 一种均相特异性检测核酸的探针及应用方法
GB0029617D0 (en) * 2000-12-05 2001-01-17 Norchip As Ligand detection method
EP1709200B1 (de) 2003-12-03 2012-05-09 Abbott Laboratories Doppelsträngige lineare nukleinsäuresonde und deren verwendung
US7494776B2 (en) * 2005-07-07 2009-02-24 Beckman Coulter, Inc. Labeled complementary oligonucleotides to detect oligonucleotide-linked ligands
EP1945359B1 (de) * 2005-09-06 2014-06-11 Thermo Fisher Scientific Oy Probenplatte und verfahren zur verarbeitung von biologischen proben
WO2007033171A2 (en) 2005-09-12 2007-03-22 Research & Diagnostic Systems, Inc. Mycoplasma detection method and composition
US20070212775A1 (en) * 2006-01-13 2007-09-13 Finnzymes Instruments Oy Microtiter plate, method of manufacturing thereof and kit
US7501254B2 (en) * 2006-07-20 2009-03-10 Ghc Technologies, Inc. Methods and compositions for amplification and capture of nucleic acid sequences
WO2010120686A2 (en) * 2009-04-13 2010-10-21 Parsons J Kellogg Methods for the diagnosis and treatment of benign prostatic hyperplasia
WO2012021741A2 (en) * 2010-08-12 2012-02-16 Clontech Laboratories, Inc. Lateral flow assays for non-diagnostic analytes
US9133509B2 (en) * 2012-03-22 2015-09-15 Lgc Genomics Limited Polymerase chain reaction detection system
US9593368B2 (en) 2014-07-01 2017-03-14 General Electric Company Methods for amplifying nucleic acids on substrates
US10870845B2 (en) 2014-07-01 2020-12-22 Global Life Sciences Solutions Operations UK Ltd Methods for capturing nucleic acids
US10472620B2 (en) 2014-07-01 2019-11-12 General Electric Company Method, substrate and device for separating nucleic acids
JP2018500932A (ja) * 2014-12-22 2018-01-18 アナーパ バイオテック エー／エス 標的核酸のマルチプレックス検出のためのデュアルクエンチングアッセイ
JP7366247B2 (ja) 2020-04-14 2023-10-20 Ｐｈｃホールディングス株式会社 核酸検査装置および核酸検査方法
WO2021263013A1 (en) * 2020-06-25 2021-12-30 President And Fellows Of Harvard College Multiplex, sensitive and rapid nucleic acid detection
EP3950955A1 (de) * 2020-08-06 2022-02-09 Albert-Ludwigs-Universität Freiburg Multianalyt-test zum gleichzeitigen nachweis von nukleinsäuren und analyten

Family Cites Families (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4683202A (en) * 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US5310650A (en) * 1986-09-29 1994-05-10 Abbott Laboratoires Method and device for improved reaction kinetics in nucleic acid hybridizations
US5210015A (en) * 1990-08-06 1993-05-11 Hoffman-La Roche Inc. Homogeneous assay system using the nuclease activity of a nucleic acid polymerase
DK0567635T3 (da) * 1991-11-15 2000-09-25 Igen Int Inc Hurtige assays for amplifikationsprodukter
WO1994002634A1 (en) * 1992-07-24 1994-02-03 University Of South Australia Amplification and detection process
JP4372837B2 (ja) * 1992-08-04 2009-11-25 ジェン−プローブ・インコーポレイテッド 核酸配列増幅
DE4344742A1 (de) * 1993-06-09 1994-12-15 Boehringer Mannheim Gmbh Verfahren zur Immobilisierung von Nukleinsäuren
EP2423322A1 (de) * 1993-11-12 2012-02-29 PHRI Properties, Inc. Hybridisierungsprobe zur Nukleinsäuredetektion, universelle Stammstrukturen, Methoden und Reagentiensätze
US5925517A (en) * 1993-11-12 1999-07-20 The Public Health Research Institute Of The City Of New York, Inc. Detectably labeled dual conformation oligonucleotide probes, assays and kits
WO1997007235A2 (en) * 1995-08-14 1997-02-27 Abbott Laboratories All-in-one nucleic acid amplification assay
US5619766A (en) * 1996-02-15 1997-04-15 Zhadanov; Sam Water driven cleaning device
AU713667B2 (en) * 1996-04-12 1999-12-09 Phri Properties, Inc. Detection probes, kits and assays
US6037130A (en) * 1998-07-28 2000-03-14 The Public Health Institute Of The City Of New York, Inc. Wavelength-shifting probes and primers and their use in assays and kits

Also Published As

Publication number Publication date
US20030087271A1 (en) 2003-05-08
WO1997032044A1 (en) 1997-09-04
EP0892856A1 (de) 1999-01-27
DE69734576T2 (de) 2006-08-03
EP0892856B1 (de) 2005-11-09
CA2246238A1 (en) 1997-09-04
DE69734576D1 (de) 2005-12-15

Similar Documents

Publication Publication Date Title
DE69734576D1 (de) Methode zur vervielfältigung und zum nachweis eines gewünschten nukleinsäurefragments
DE69533771D1 (de) Verfahren zum Einfangen und selektivem Freisetzen von Nukleinsäuren unter Verwendung eines schwach basischen Polymers und Amplifikation dieser Nukleinsäuren
ATE353372T1 (de) Verfahen zum nachweis von nukleinsäure mit einer spezifischen sequenzzusammensetzung
ATE333517T1 (de) Verfahren und sondenset zur erkennung von krebs
DE69708865D1 (de) Verfahren zur direkten sequenzierung während "template"-amplifikation
DE60041355D1 (de) Verfahren zur detektion von erkrankungen
DE60128908D1 (de) Verfahren zum Nachweis und zur Lokalisierung von Genen in situ durch Hybridisierung einer verzweigten DNA
ATE106452T1 (de) Verfahren zum kontrollieren und/oder überprüfen von biologischen prozessen.
NO910183L (no) Fremgangsmaate ved paavisning av vannbaarne mikrobiske patogener og indikatorer paa human fecal kontaminering i vannproever og tilhoerende kits.
ATE340868T1 (de) Zwei-schritt hybridisierung und einfang von einem polynukleotid
ATE236400T1 (de) Vorrichtung und verfahren zum nachweis von zielliganden
FR2737502B1 (fr) Procede de detection d'acides nucleiques utilisant des sondes nucleotidiques permettant a la fois une capture specifique et une detection
ATE98300T1 (de) Verfahren zum aufspueren, identifizieren und quantifizieren von organismen und viren.
ATE219151T1 (de) Verfahren zur quantifizierung von tumorzellen in einer körperflüssigkeit und dazu geeignete testkits
DE69534301D1 (de) Kulturmedium und vorrichtung zum nachweis und zur quantifizierung von enterobakteriaceae
ATE170001T1 (de) Vorrichtung und verfahren zur überführung einer probe eines fluids
ATE206764T1 (de) Verfahren zum sensitiven nachweis von nukleinsäuren
MXPA03001879A (es) Metodo y sistema para detectar acidos nucleicos.
ATE360092T1 (de) Nukleinsäureprimer und -sonden zur detektion onkogener humaner papillomaviren
CA2140877A1 (en) Amplification and detection process
DE69632252D1 (de) Verfahren zur erkennung von klonalen populationen von transformierten zellen in einer genomisch heterogenen zellulären probe
DE68912342D1 (de) Verfahren zum nachweis des menschlichen papillomavirus (hpv) für diagnosezwecke.
ATE318930T1 (de) Nukleinsäuresonden zur detektion von candida- spezies
ATE40571T1 (de) Verfahren und mittel zur durchfuehrung mikrochemischer und mikrobiologischer tests.
DE68919387D1 (de) DNS- und RNS-Proben, Methoden und Testsätze zum Nachweis von Salmonella.

Legal Events

Date Code Title Description
RER Ceased as to paragraph 5 lit. 3 law introducing patent treaties