ATE309387T1 - Methode zur vervielfältigung und zum nachweis eines gewünschten nukleinsäurefragments - Google Patents

Methode zur vervielfältigung und zum nachweis eines gewünschten nukleinsäurefragments

Info

Publication number
ATE309387T1
ATE309387T1 AT97914790T AT97914790T ATE309387T1 AT E309387 T1 ATE309387 T1 AT E309387T1 AT 97914790 T AT97914790 T AT 97914790T AT 97914790 T AT97914790 T AT 97914790T AT E309387 T1 ATE309387 T1 AT E309387T1
Authority
AT
Austria
Prior art keywords
probe
nucleic acid
detection
analyte
duplication
Prior art date
Application number
AT97914790T
Other languages
English (en)
Inventor
Richard C Ebersole
Edwin R Hendrickson
Sandra Fitzpatrick-Mcelligott
Michael P Perry
Original Assignee
Du Pont
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Du Pont filed Critical Du Pont
Application granted granted Critical
Publication of ATE309387T1 publication Critical patent/ATE309387T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
AT97914790T 1996-03-01 1997-02-27 Methode zur vervielfältigung und zum nachweis eines gewünschten nukleinsäurefragments ATE309387T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US1263696P 1996-03-01 1996-03-01
PCT/US1997/002892 WO1997032044A1 (en) 1996-03-01 1997-02-27 A method for the amplification and detection of a nucleic acid fragment of interest

Publications (1)

Publication Number Publication Date
ATE309387T1 true ATE309387T1 (de) 2005-11-15

Family

ID=21755944

Family Applications (1)

Application Number Title Priority Date Filing Date
AT97914790T ATE309387T1 (de) 1996-03-01 1997-02-27 Methode zur vervielfältigung und zum nachweis eines gewünschten nukleinsäurefragments

Country Status (6)

Country Link
US (1) US20030087271A1 (de)
EP (1) EP0892856B1 (de)
AT (1) ATE309387T1 (de)
CA (1) CA2246238A1 (de)
DE (1) DE69734576T2 (de)
WO (1) WO1997032044A1 (de)

Families Citing this family (22)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20030040114A1 (en) * 1996-09-05 2003-02-27 Warner-Lambert Company, Small molecule modulation of ribozymes
US7803528B1 (en) 1997-02-28 2010-09-28 Quest Diagnostics Incorporated Fluorescence energy transfer by competitive hybridization
GB9807045D0 (en) * 1998-04-01 1998-06-03 Rudi Knut Nucleic acid detection method
DE60028233T2 (de) * 1999-10-27 2007-03-08 Universite De Liege Immuno-real-time-pcr unter verwendung einer chimären dna als amplifikationsmarker
US7306904B2 (en) * 2000-02-18 2007-12-11 Olink Ab Methods and kits for proximity probing
CN1348096A (zh) * 2000-10-10 2002-05-08 栾国彦 一种均相特异性检测核酸的探针及应用方法
GB0029617D0 (en) 2000-12-05 2001-01-17 Norchip As Ligand detection method
ES2385485T3 (es) 2003-12-03 2012-07-25 Abbott Laboratories Sonda de ácido nucleico lineal de doble hebra y usos de la misma
US7494776B2 (en) * 2005-07-07 2009-02-24 Beckman Coulter, Inc. Labeled complementary oligonucleotides to detect oligonucleotide-linked ligands
JP4977708B2 (ja) * 2005-09-06 2012-07-18 フィンザイムズ・オサケユキテュア サンプルプレートアセンブリおよび生体サンプルを処理する方法
US7855051B2 (en) 2005-09-12 2010-12-21 Research & Diagnostic Systems, Inc. Mycoplasma detection method and composition
US20070212775A1 (en) * 2006-01-13 2007-09-13 Finnzymes Instruments Oy Microtiter plate, method of manufacturing thereof and kit
US7501254B2 (en) * 2006-07-20 2009-03-10 Ghc Technologies, Inc. Methods and compositions for amplification and capture of nucleic acid sequences
WO2010120686A2 (en) * 2009-04-13 2010-10-21 Parsons J Kellogg Methods for the diagnosis and treatment of benign prostatic hyperplasia
EP2603799A4 (de) * 2010-08-12 2014-02-12 Clontech Lab Inc Seitenflussassays für nicht-diagnostische analyte
US9133509B2 (en) * 2012-03-22 2015-09-15 Lgc Genomics Limited Polymerase chain reaction detection system
US10472620B2 (en) 2014-07-01 2019-11-12 General Electric Company Method, substrate and device for separating nucleic acids
US9593368B2 (en) 2014-07-01 2017-03-14 General Electric Company Methods for amplifying nucleic acids on substrates
US10870845B2 (en) 2014-07-01 2020-12-22 Global Life Sciences Solutions Operations UK Ltd Methods for capturing nucleic acids
DK3237636T3 (da) * 2014-12-22 2021-01-25 Anapa Biotech As Dobbelt-quenching analyse til multiplex detektion af målnukleinsyrer
WO2021210298A1 (ja) * 2020-04-14 2021-10-21 Ｐｈｃホールディングス株式会社 核酸検査装置および核酸検査方法
WO2021263013A1 (en) * 2020-06-25 2021-12-30 President And Fellows Of Harvard College Multiplex, sensitive and rapid nucleic acid detection

Family Cites Families (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4683202A (en) * 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US5310650A (en) * 1986-09-29 1994-05-10 Abbott Laboratoires Method and device for improved reaction kinetics in nucleic acid hybridizations
US5210015A (en) * 1990-08-06 1993-05-11 Hoffman-La Roche Inc. Homogeneous assay system using the nuclease activity of a nucleic acid polymerase
CA2100159C (en) * 1991-11-15 2003-01-21 John R. Link Rapid assays for amplification products
US5849544A (en) * 1992-07-24 1998-12-15 University Of Australia Amplification and detection process
CA2141430C (en) * 1992-08-04 2009-05-12 Sherrol H. Mcdonough Nucleic acid sequence amplification
DE4344742A1 (de) * 1993-06-09 1994-12-15 Boehringer Mannheim Gmbh Verfahren zur Immobilisierung von Nukleinsäuren
DK1921169T3 (da) * 1993-11-12 2012-06-04 Phri Properties Inc Hybridiseringssonder til nukleinsyredetektion, universelle stamceller, fremgangsmåder og kits
US5925517A (en) * 1993-11-12 1999-07-20 The Public Health Research Institute Of The City Of New York, Inc. Detectably labeled dual conformation oligonucleotide probes, assays and kits
JP3418622B2 (ja) * 1995-08-14 2003-06-23 アボツト・ラボラトリーズ オールインワン核酸増幅アッセイ
US5619766A (en) * 1996-02-15 1997-04-15 Zhadanov; Sam Water driven cleaning device
AU713667B2 (en) * 1996-04-12 1999-12-09 Phri Properties, Inc. Detection probes, kits and assays
US6037130A (en) * 1998-07-28 2000-03-14 The Public Health Institute Of The City Of New York, Inc. Wavelength-shifting probes and primers and their use in assays and kits

Also Published As

Publication number Publication date
CA2246238A1 (en) 1997-09-04
EP0892856B1 (de) 2005-11-09
US20030087271A1 (en) 2003-05-08
EP0892856A1 (de) 1999-01-27
WO1997032044A1 (en) 1997-09-04
DE69734576D1 (de) 2005-12-15
DE69734576T2 (de) 2006-08-03

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