ATE314465T1 - Verfahren zum screening von hochaktiven proteasen und hemmstoffen - Google Patents

Verfahren zum screening von hochaktiven proteasen und hemmstoffen

Info

Publication number
ATE314465T1
ATE314465T1 AT01960178T AT01960178T ATE314465T1 AT E314465 T1 ATE314465 T1 AT E314465T1 AT 01960178 T AT01960178 T AT 01960178T AT 01960178 T AT01960178 T AT 01960178T AT E314465 T1 ATE314465 T1 AT E314465T1
Authority
AT
Austria
Prior art keywords
protease
gene
inhibitors
highly active
active proteases
Prior art date
Application number
AT01960178T
Other languages
English (en)
Inventor
Poul Erik Pedersen
Mads Noerregaard-Madsen
Original Assignee
Novozymes As
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Novozymes As filed Critical Novozymes As
Application granted granted Critical
Publication of ATE314465T1 publication Critical patent/ATE314465T1/de

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/48Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6402Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from non-mammals
    • C12N9/6405Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from non-mammals not being snakes
    • C12N9/6408Serine endopeptidases (3.4.21)
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K14/00Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/81Protease inhibitors
    • C07K14/8107Endopeptidase (E.C. 3.4.21-99) inhibitors
    • C07K14/811Serine protease (E.C. 3.4.21) inhibitors
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034Isolating an individual clone by screening libraries
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/025Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • C12Q1/37Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving peptidase or proteinase

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biochemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Immunology (AREA)
  • Analytical Chemistry (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Toxicology (AREA)
  • Plant Pathology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Detergent Compositions (AREA)
AT01960178T 2000-08-29 2001-07-17 Verfahren zum screening von hochaktiven proteasen und hemmstoffen ATE314465T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DKPA200001273 2000-08-29
PCT/DK2001/000503 WO2002018588A1 (en) 2000-08-29 2001-07-17 Method for screening highly active proteases and inhibitors

Publications (1)

Publication Number Publication Date
ATE314465T1 true ATE314465T1 (de) 2006-01-15

Family

ID=8159678

Family Applications (1)

Application Number Title Priority Date Filing Date
AT01960178T ATE314465T1 (de) 2000-08-29 2001-07-17 Verfahren zum screening von hochaktiven proteasen und hemmstoffen

Country Status (7)

Country Link
US (1) US20040038375A1 (de)
EP (1) EP1315806B1 (de)
AT (1) ATE314465T1 (de)
AU (1) AU2001281734A1 (de)
CA (1) CA2419860A1 (de)
DE (1) DE60116337D1 (de)
WO (1) WO2002018588A1 (de)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20080090745A1 (en) * 2006-10-13 2008-04-17 Fox Bryan P Expression of Streptomyces subtilism inhibitor (SSI) proteins in Bacillus and Streptomyces sp.
US9403897B2 (en) 2011-10-12 2016-08-02 Sanyo Chemical Industries, Ltd. Proteinaceous protease inhibitor and protein solution and detergent composition containing it
JP6591998B2 (ja) * 2014-03-28 2019-10-16 ノボザイムス アクティーゼルスカブ 低pHでのタンパク質結晶の再可溶化
US12019066B2 (en) * 2016-05-16 2024-06-25 Biomadison, Inc. Assay with synaptobrevin based moiety
WO2017201079A1 (en) 2016-05-16 2017-11-23 Biomadison, Inc. Improved assay with synaptobrevin based moiety
WO2020074517A1 (en) * 2018-10-10 2020-04-16 Novozymes A/S Chymotrypsin inhibitor variants and the use thereof
CA3257053A1 (en) 2022-05-14 2023-11-23 Novozymes A/S COMPOSITIONS AND METHODS FOR THE PREVENTION, TREATMENT, SUPPRESSION AND/OR ELIMINATION OF PHYTOPATHOGENIC INFESTATIONS AND INFECTIONS

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2377392A (en) * 1991-07-09 1993-02-11 Robert Balint A method for the identification of protease inhibitors
US6159688A (en) * 1997-03-18 2000-12-12 Novo Nordisk A/S Methods of producing polynucleotide variants
CA2360022A1 (en) * 1999-01-08 2000-07-13 Bristol-Myers Squibb Company Prokaryotic system designed to monitor protease activity

Also Published As

Publication number Publication date
WO2002018588A1 (en) 2002-03-07
CA2419860A1 (en) 2002-03-07
EP1315806A1 (de) 2003-06-04
EP1315806B1 (de) 2005-12-28
AU2001281734A1 (en) 2002-03-13
DE60116337D1 (de) 2006-02-02
US20040038375A1 (en) 2004-02-26

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