ATE317545T1 - Anordnung und verfahren zum feststellen und/oder überwachen elektrophysiologischer eigenschaften von ionenkanälen - Google Patents

Anordnung und verfahren zum feststellen und/oder überwachen elektrophysiologischer eigenschaften von ionenkanälen

Info

Publication number
ATE317545T1
ATE317545T1 AT00962271T AT00962271T ATE317545T1 AT E317545 T1 ATE317545 T1 AT E317545T1 AT 00962271 T AT00962271 T AT 00962271T AT 00962271 T AT00962271 T AT 00962271T AT E317545 T1 ATE317545 T1 AT E317545T1
Authority
AT
Austria
Prior art keywords
measuring
cell
electrode
substrate
determining
Prior art date
Application number
AT00962271T
Other languages
English (en)
Inventor
Jon Wulff Petersen
Pieter Telleman
Ole Hansen
Palle Christophersen
Morten Bech
Soren Peter Olesen
Jorgen Due
Lars Thomsen
Original Assignee
Sophion Bioscience As
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Sophion Bioscience As filed Critical Sophion Bioscience As
Application granted granted Critical
Publication of ATE317545T1 publication Critical patent/ATE317545T1/de

Links

Classifications

    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/483Physical analysis of biological material
    • G01N33/487Physical analysis of biological material of liquid biological material
    • G01N33/48707Physical analysis of biological material of liquid biological material by electrical means
    • G01N33/48728Investigating individual cells, e.g. by patch clamp, voltage clamp

Landscapes

  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Food Science & Technology (AREA)
  • Biochemistry (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Investigating Or Analyzing Materials By The Use Of Electric Means (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
AT00962271T 1999-10-01 2000-10-02 Anordnung und verfahren zum feststellen und/oder überwachen elektrophysiologischer eigenschaften von ionenkanälen ATE317545T1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
DKPA199901407 1999-10-01

Publications (1)

Publication Number Publication Date
ATE317545T1 true ATE317545T1 (de) 2006-02-15

Family

ID=8104506

Family Applications (1)

Application Number Title Priority Date Filing Date
AT00962271T ATE317545T1 (de) 1999-10-01 2000-10-02 Anordnung und verfahren zum feststellen und/oder überwachen elektrophysiologischer eigenschaften von ionenkanälen

Country Status (11)

Country Link
EP (1) EP1221046B1 (de)
JP (1) JP4861584B2 (de)
CN (1) CN1204404C (de)
AT (1) ATE317545T1 (de)
AU (1) AU780157B2 (de)
CA (1) CA2385482C (de)
DE (1) DE60025929T2 (de)
DK (1) DK1221046T3 (de)
IL (1) IL148650A0 (de)
NZ (1) NZ518057A (de)
WO (1) WO2001025769A2 (de)

Families Citing this family (91)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20020144905A1 (en) 1997-12-17 2002-10-10 Christian Schmidt Sample positioning and analysis system
US7244349B2 (en) 1997-12-17 2007-07-17 Molecular Devices Corporation Multiaperture sample positioning and analysis system
DE19948473A1 (de) 1999-10-08 2001-04-12 Nmi Univ Tuebingen Verfahren und Vorrichtung zum Messen an in einer flüssigen Umgebung befindlichen Zellen
FR2802078B1 (fr) * 1999-12-14 2003-10-03 Univ Joseph Fourier Microelectrode support de cellules a membrane excitable
WO2002004943A2 (en) * 2000-07-07 2002-01-17 Bristol-Myers Squibb Company Electrophysiology configuration suitable for high throughput screening of compounds for drug discovery
US7270730B2 (en) 2000-08-04 2007-09-18 Essen Instruments, Inc. High-throughput electrophysiological measurement system
US7067046B2 (en) 2000-08-04 2006-06-27 Essen Instruments, Inc. System for rapid chemical activation in high-throughput electrophysiological measurements
AU2001287472A1 (en) * 2000-09-19 2002-04-02 Cytion Sa Sample positioning and analysis system
US6932893B2 (en) 2000-10-02 2005-08-23 Sophion Bioscience A/S System for electrophysiological measurements
AU9367601A (en) * 2000-10-02 2002-04-15 Sophion Bioscience As System for electrophysiological measurements
US6776896B1 (en) 2000-10-11 2004-08-17 Axon Instruments, Inc. Method of positioning cells for electrophysiological testing
WO2002055653A1 (en) * 2001-01-09 2002-07-18 Matsushita Electric Industrial Co., Ltd. Device for measuring extracellular potential, method of measuring extracellular potential by using the same and apparatus for quickly screening drug provided therewith
AU2002307218A1 (en) 2001-03-24 2002-10-08 Aviva Biosciences Corporation Biochips including ion transport detecting structures and methods of use
US20060029955A1 (en) 2001-03-24 2006-02-09 Antonio Guia High-density ion transport measurement biochip devices and methods
WO2002103354A1 (en) * 2001-06-20 2002-12-27 Sophion Bioscience A/S An apparatus and method for determining and/or monitoring electrophysiological properties of ion channels
US6899800B2 (en) 2001-06-20 2005-05-31 Axon Instruments, Inc. Polymeric electrode for electrophysiological testing
JP2003043009A (ja) 2001-07-30 2003-02-13 Matsushita Electric Ind Co Ltd 生体試料の発する物理化学的変化を検出するデバイスおよび装置
DE10142393C1 (de) * 2001-08-30 2003-01-23 Fraunhofer Ges Forschung Vorrichtung und Verfahren zur Erfassung bioelektrischer Signale aus elektrophysiologisch aktiven Bereichen in Sphäroiden
DE10202887B4 (de) * 2002-01-25 2004-05-06 Advalytix Ag Zellanalyseverfahren
US8003372B2 (en) 2002-02-05 2011-08-23 The University Court Of The University Of Glasgow Device for performing cell assays
ATE516879T1 (de) 2002-02-12 2011-08-15 Cellectricon Ab Systeme und verfahren zur schnellen änderung der lösungsumgebung von sensoren
US7470518B2 (en) 2002-02-12 2008-12-30 Cellectricon Ab Systems and method for rapidly changing the solution environment around sensors
GB0207845D0 (en) * 2002-04-04 2002-05-15 Lgc Ltd Apparatus, methods and means for biochemical analysis
DK1495105T3 (da) * 2002-04-17 2007-06-04 Sophion Bioscience As Substrat og fremgangsmåde til måling af elektrofysiologiske egenskaber for cellemenbraner
GB2398635A (en) * 2003-02-21 2004-08-25 Sophion Bioscience As A substrate providing a cell gigaseal for a patch clamp
AU2003231300A1 (en) 2002-05-04 2003-11-17 Aviva Biosciences Corporation Apparatus including ion transport detecting structures and methods of use
WO2003096002A1 (en) * 2002-05-13 2003-11-20 Matsushita Electric Industrial Co., Ltd. Instrument and method for measuring action signal of biological sample
ES2208082B1 (es) * 2002-05-29 2005-09-16 Jose Luis Bardasano Rubio Dispositivo para la medida del potencial de la accion transmembrana en preparaciones celulares bajo la accion de campos electromagneticos de frecuencia e intensidad variables.
JP3861831B2 (ja) * 2003-03-07 2006-12-27 松下電器産業株式会社 細胞外電位測定デバイスおよびその製造方法
JP3925439B2 (ja) * 2003-03-07 2007-06-06 松下電器産業株式会社 細胞外電位測定デバイスおよびその製造方法
JP4552423B2 (ja) * 2003-11-21 2010-09-29 パナソニック株式会社 細胞外電位測定デバイスおよびこれを用いた細胞外電位の測定方法
WO2006132116A1 (ja) 2005-06-07 2006-12-14 Matsushita Electric Industrial Co., Ltd. 細胞電気生理測定デバイスおよびその製造方法
US8257962B2 (en) 2003-03-07 2012-09-04 Panasonic Corporation Extracellular potential measuring device and its manufacturing method
US8202439B2 (en) 2002-06-05 2012-06-19 Panasonic Corporation Diaphragm and device for measuring cellular potential using the same, manufacturing method of the diaphragm
WO2009034697A1 (ja) 2007-09-11 2009-03-19 Panasonic Corporation シリコン構造体およびその製造方法並びにセンサチップ
JP3945317B2 (ja) * 2002-06-05 2007-07-18 松下電器産業株式会社 細胞外電位測定デバイスおよびその製造方法
EP1411351A4 (de) * 2002-06-05 2010-07-07 Panasonic Corp Vorrichtung zur messung des extrazellulären potenzials und verfahren zur herstellung der vorrichtung
JP3945338B2 (ja) * 2002-08-01 2007-07-18 松下電器産業株式会社 細胞外電位測定デバイスおよびその製造方法
DK1511843T3 (da) * 2002-06-07 2006-07-24 Sophion Bioscience As Screeningsmetoder
US7468255B2 (en) 2002-12-20 2008-12-23 Acea Biosciences Method for assaying for natural killer, cytotoxic T-lymphocyte and neutrophil-mediated killing of target cells using real-time microelectronic cell sensing technology
WO2005077104A2 (en) * 2004-02-09 2005-08-25 Xiao Xu Real-time electronic cell sensing system and applications for cytotoxicity profiling and compound assays
US7470533B2 (en) 2002-12-20 2008-12-30 Acea Biosciences Impedance based devices and methods for use in assays
US7732127B2 (en) 2002-12-20 2010-06-08 Acea Biosciences, Inc. Dynamic monitoring of cell adhesion and spreading using the RT-CES system
US8263375B2 (en) 2002-12-20 2012-09-11 Acea Biosciences Dynamic monitoring of activation of G-protein coupled receptor (GPCR) and receptor tyrosine kinase (RTK) in living cells using real-time microelectronic cell sensing technology
US8206903B2 (en) 2002-12-20 2012-06-26 Acea Biosciences Device and method for electroporation-based delivery of molecules into cells and dynamic monitoring of cell responses
US7560269B2 (en) 2002-12-20 2009-07-14 Acea Biosciences, Inc. Real time electronic cell sensing system and applications for cytotoxicity profiling and compound assays
CA2493101C (en) 2002-07-20 2012-05-08 Acea Biosciences, Inc. Impedance based apparatuses and methods for analyzing cells and particles
WO2005047482A2 (en) 2003-11-12 2005-05-26 Xiao Xu Real time electronic cell sensing systems and applications for cell-based assays
US7810380B2 (en) 2003-03-25 2010-10-12 Tearlab Research, Inc. Systems and methods for collecting tear film and measuring tear film osmolarity
EP1801586B1 (de) * 2002-08-21 2010-10-13 Cellectricon Ab System zur Gewinnung und Erhaltung hochresistenter Verschlüsse bei Patch-Clamp-Aufzeichnungen
JP4386835B2 (ja) 2002-08-21 2009-12-16 セレクトリコン アーベー パッチクランプ記録において高抵抗固着を獲得して維持するシステム及び方法
FR2844052B1 (fr) 2002-08-28 2005-07-01 Commissariat Energie Atomique Dispositif de mesure de l'activite electrique d'elements biologiques et ses applications
AU2003278461A1 (en) 2002-10-16 2004-05-04 Cellectricon Ab Nanoelectrodes and nanotips for recording transmembrane currents in a plurality of cells
US10551371B2 (en) 2003-11-10 2020-02-04 Acea Biosciences, Inc. System and method for monitoring cardiomyocyte beating, viability and morphology and for screening for pharmacological agents which may induce cardiotoxicity or modulate cardiomyocyte function
US10215748B2 (en) 2002-12-20 2019-02-26 Acea Biosciences, Inc. Using impedance-based cell response profiling to identify putative inhibitors for oncogene addicted targets or pathways
US10539523B2 (en) 2002-12-20 2020-01-21 Acea Biosciences, Inc. System and method for monitoring cardiomyocyte beating, viability, morphology, and electrophysiological properties
US11346797B2 (en) 2002-12-20 2022-05-31 Agilent Technologies, Inc. System and method for monitoring cardiomyocyte beating, viability, morphology and electrophysiological properties
GB0303920D0 (en) * 2003-02-21 2003-03-26 Sophion Bioscience As Capillary stop
WO2004109282A1 (en) * 2003-06-10 2004-12-16 Yissum Research Development Company Of The Hebrew University Of Jerusalem Electronic device for communication with living cells
FR2866958B1 (fr) 2004-02-26 2006-08-04 Commissariat Energie Atomique Procede et dispositif de controle du positionnement d'un element biologique sur un support
GB2431013B (en) * 2004-07-23 2008-05-21 Electronic Bio Sciences Llc Method and apparatus for sensing a time varying current passing through an ion channel
JPWO2006022092A1 (ja) 2004-08-25 2008-05-08 松下電器産業株式会社 細胞電位測定プローブ
JP2006184207A (ja) * 2004-12-28 2006-07-13 Matsushita Electric Ind Co Ltd 細胞膜の特性および状態の少なくとも一方の電気的測定方法および電気的測定装置
US20060216203A1 (en) * 2005-03-28 2006-09-28 Mds Sciex (Us) A Division Of Mds Pharma Services (Us) Inc. Multiwell sample plate with integrated impedance electrodes and connection scheme
JP4661539B2 (ja) * 2005-11-11 2011-03-30 パナソニック株式会社 細胞電気生理センサおよびその製造方法
US7608417B2 (en) * 2005-11-14 2009-10-27 Panasonic Corporation Cell electro-physiological sensor and method of manufacturing the same
JP4470999B2 (ja) 2005-12-20 2010-06-02 パナソニック株式会社 細胞電気生理センサ
JP2009156572A (ja) * 2006-04-06 2009-07-16 National Institutes Of Natural Sciences イオンチャンネルタンパク質バイオセンサー
US8071363B2 (en) 2006-05-25 2011-12-06 Panasonic Corporation Chip for cell electrophysiological sensor, cell electrophysiological sensor using the same, and manufacturing method of chip for cell electrophysiological sensor
EP2037258A4 (de) 2006-07-06 2013-12-04 Panasonic Corp Vorrichtung für zellelektrophysiologiesensor, zellelektrophysiologiesensor unter verwendung der vorrichtung sowie verfahren zur herstellung der zellelektrophysiologiesensorvorrichtung
US8041515B2 (en) 2006-09-20 2011-10-18 Acea Biosciences, Inc. Use of impedance-based cytological profiling to classify cellular response profiles upon exposure to biologically active agents
US8163527B2 (en) * 2007-05-04 2012-04-24 Tecella Llc Subsystems and methods for use in patch clamp systems
WO2009137440A1 (en) 2008-05-05 2009-11-12 Acea Biosciences, Inc. Label-free monitoring of excitation-contraction coupling and excitable cells using impedance based systems with millisecond time resolution
WO2011146531A1 (en) 2010-05-18 2011-11-24 Acea Biosciences, Inc Data analysis of impedance-based cardiomyocyte-beating signals as detected on real-time cell analysis (rtca) cardio instruments
US20130136673A1 (en) 2010-07-09 2013-05-30 Sophion Bioscience A/S Chip assembly for use in a microfluidic analysis sytem
JP5575623B2 (ja) * 2010-12-03 2014-08-20 マルボシ酢株式会社 誘電特性測定用耐圧容器センサーに用いる絶縁体
JP6037717B2 (ja) * 2011-12-20 2016-12-07 国立研究開発法人科学技術振興機構 プレーナーパッチクランプ装置、該装置用電極部及び細胞イオンチャンネル電流計測方法
CA2860501C (en) * 2012-01-09 2021-05-18 Sophion Bioscience A/S Improved patch area cell adhesion
DE102013007295B4 (de) * 2013-04-26 2015-06-25 Universität Rostock Elektrophysiologische Messanordnung und elektrophysiologisches Messverfahren
EP3040410B1 (de) 2013-08-30 2018-04-25 Japan Science and Technology Agency Flache patch-clamp-vorrichtung und verfahren verwendend die patch-clamp vorrichtung
US11536707B2 (en) 2014-09-23 2022-12-27 Tearlab Research, Inc. Systems and methods for integration of microfluidic tear collection and lateral flow analysis of analytes of interest
US12066428B2 (en) 2015-11-20 2024-08-20 Agilent Technologies, Inc. Cell-substrate impedance monitoring of cancer cells
JP6676486B2 (ja) * 2016-04-05 2020-04-08 シャープ株式会社 検出方法
WO2017175879A1 (ja) * 2016-04-05 2017-10-12 シャープ株式会社 センサ装置、検出方法、及びセンサユニット
US11746328B2 (en) 2017-03-03 2023-09-05 Agilent Technologies, Inc. Methods and systems for functional maturation of iPSC and ESC derived cardiomyocytes
TWM565302U (zh) * 2017-11-03 2018-08-11 國立臺灣大學 一種多離子感測電極陣列試片及其感測裝置
JP7229110B2 (ja) 2019-06-25 2023-02-27 株式会社Screenホールディングス 細胞電位測定装置
JP7469092B2 (ja) 2020-03-23 2024-04-16 株式会社Screenホールディングス 細胞保持容器
US20210301245A1 (en) 2020-03-29 2021-09-30 Agilent Technologies, Inc. Systems and methods for electronically and optically monitoring biological samples
WO2023131698A1 (en) 2022-01-07 2023-07-13 Sophion Bioscience A/S Semi-automated patch-clamp apparatus and a method for carrying out a patch-clamp procedure
WO2023131694A1 (en) 2022-01-07 2023-07-13 Sophion Bioscience A/S Pipette guidance in multiple-well plate patch-clamp

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4062750A (en) * 1974-12-18 1977-12-13 James Francis Butler Thin film electrochemical electrode and cell
US4225410A (en) * 1978-12-04 1980-09-30 Technicon Instruments Corporation Integrated array of electrochemical sensors
US4874500A (en) * 1987-07-15 1989-10-17 Sri International Microelectrochemical sensor and sensor array
US4812221A (en) * 1987-07-15 1989-03-14 Sri International Fast response time microsensors for gaseous and vaporous species
DE4115414C2 (de) * 1991-05-10 1995-07-06 Meinhard Prof Dr Knoll Verfahren zur Herstellung von miniaturisierten Chemo- und Biosensorelementen mit ionenselektiver Membran sowie von Trägern für diese Elemente
IL103674A0 (en) * 1991-11-19 1993-04-04 Houston Advanced Res Center Method and apparatus for molecule detection
US6758961B1 (en) * 1997-12-17 2004-07-06 Ecole Polytechnique Federale De Lausanne Positioning and electrophysiological characterization of individual cells and reconstituted membrane systems on microstructured carriers

Also Published As

Publication number Publication date
CA2385482C (en) 2011-01-18
DE60025929T2 (de) 2006-11-09
DK1221046T3 (da) 2006-06-12
NZ518057A (en) 2004-01-30
JP2003511668A (ja) 2003-03-25
EP1221046A2 (de) 2002-07-10
WO2001025769A3 (en) 2001-08-30
WO2001025769A2 (en) 2001-04-12
CA2385482A1 (en) 2001-04-12
CN1377464A (zh) 2002-10-30
AU7406500A (en) 2001-05-10
DE60025929D1 (de) 2006-04-20
JP4861584B2 (ja) 2012-01-25
CN1204404C (zh) 2005-06-01
EP1221046B1 (de) 2006-02-08
IL148650A0 (en) 2002-09-12
AU780157B2 (en) 2005-03-03

Similar Documents

Publication Publication Date Title
DE60025929D1 (de) Anordnung und verfahren zum feststellen und/oder überwachen elektrophysiologischer eigenschaften von ionenkanälen
JP3093274B2 (ja) 気体濃度測定の方法及びそれを実施するための微細加工検出装置
DE60330385D1 (de) Verfahren und vorrichtung zum einfangen geladener moleküle eines stromes
US2913386A (en) Electrochemical device for chemical analysis
GB0801142D0 (en) Label-free molecule detection and measurement
WO2005026178A3 (en) Electrochemical affinity biosensor system and methods
CA2424498A1 (en) System for electrophysiological measurements
MXPA03000160A (es) Configuracion de electrofisiologia adecuada para separacion por exclusion de alta produccion de compuestos para el descubrimiento de farmacos.
DE60329187D1 (de) Impedanz basierende vorrichtungen und verfahren zur analyse von zellen und partikeln
DE60220035D1 (de) Sensor-array-vorrichtung sowie ein verfahren zur überwachung von elektrochemischer korrosion
GB2436774A (en) Electrically active combinatorial-chemical (EACC) chip for biochemical analyte detection
US20130168247A1 (en) Ion analyzer
JP2016508616A5 (de)
US20180125400A1 (en) Glucose measuring device and apparatus
EP4645448A4 (de) Lithium-supplementierendes material, positivelektrode, elektrochemische vorrichtung und elektrische vorrichtung
JP7330519B2 (ja) 生物学的または化学的因子試料のインピーダンス測定のためのセンサ、およびこのようなセンサを使用する、試料における生物学的または化学的因子の検出方法
DE60311973D1 (de) Substrat und verfahren zum messen elektrophysiologischer eigenschaften von zellmembranen
EP3236248B1 (de) Ionenleitfähigkeitstestvorrichtung und testverfahren damit
EP4394939A4 (de) Positivelektrodenmaterial, elektrochemische vorrichtung und elektrische vorrichtung
WO2009008334A1 (ja) イオン伝導性電解質膜の検査方法及び装置
JP2008298644A (ja) 電気泳動兼転写装置、電気泳動兼転写用チップ、ならびに電気泳動および転写方法
US7652479B2 (en) Electrolyte measurement device and measurement procedure
CN105911101B (zh) 一种简单灵敏的膜性能检测方法
US9702851B1 (en) Gel electrophoresis and transfer combination using conductive polymers and method of use
EP4517902A4 (de) Elektrolyt für natriumionenbatterie, natriumionenbatterie damit und elektrische vorrichtung

Legal Events

Date Code Title Description
RER Ceased as to paragraph 5 lit. 3 law introducing patent treaties