ATE329020T1 - Verfahren zur klonierung von genen - Google Patents

Verfahren zur klonierung von genen

Info

Publication number
ATE329020T1
ATE329020T1 AT99918302T AT99918302T ATE329020T1 AT E329020 T1 ATE329020 T1 AT E329020T1 AT 99918302 T AT99918302 T AT 99918302T AT 99918302 T AT99918302 T AT 99918302T AT E329020 T1 ATE329020 T1 AT E329020T1
Authority
AT
Austria
Prior art keywords
protein
membrane
gene encoding
cloning genes
fused
Prior art date
Application number
AT99918302T
Other languages
English (en)
Inventor
Masayuki Tsuchiya
Mikiyoshi Saito
Toshihiko Ohtomo
Original Assignee
Chugai Pharmaceutical Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Chugai Pharmaceutical Co Ltd filed Critical Chugai Pharmaceutical Co Ltd
Application granted granted Critical
Publication of ATE329020T1 publication Critical patent/ATE329020T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1051—Gene trapping, e.g. exon-, intron-, IRES-, signal sequence-trap cloning, trap vectors
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/28—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
    • C07K16/2866—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against receptors for cytokines, lymphokines, interferons
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/20—Immunoglobulins specific features characterized by taxonomic origin
    • C07K2317/24—Immunoglobulins specific features characterized by taxonomic origin containing regions, domains or residues from different species, e.g. chimeric, humanized or veneered
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/60—Immunoglobulins specific features characterized by non-natural combinations of immunoglobulin fragments
    • C07K2317/62—Immunoglobulins specific features characterized by non-natural combinations of immunoglobulin fragments comprising only variable region components
    • C07K2317/622—Single chain antibody (scFv)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Immunology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Medicinal Chemistry (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Toxicology (AREA)
  • Cell Biology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Saccharide Compounds (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
AT99918302T 1998-05-20 1999-04-30 Verfahren zur klonierung von genen ATE329020T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP13865298 1998-05-20
JP27987698 1998-10-01

Publications (1)

Publication Number Publication Date
ATE329020T1 true ATE329020T1 (de) 2006-06-15

Family

ID=26471649

Family Applications (1)

Application Number Title Priority Date Filing Date
AT99918302T ATE329020T1 (de) 1998-05-20 1999-04-30 Verfahren zur klonierung von genen

Country Status (13)

Country Link
US (3) US6610485B1 (de)
EP (1) EP1081224B1 (de)
JP (1) JP3471006B2 (de)
KR (1) KR100518958B1 (de)
CN (1) CN1310758A (de)
AT (1) ATE329020T1 (de)
AU (1) AU753310B2 (de)
CA (1) CA2331917A1 (de)
DE (1) DE69931776T2 (de)
IL (1) IL139705A0 (de)
NO (1) NO20005810L (de)
TW (1) TW593679B (de)
WO (1) WO1999060113A1 (de)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
IL139705A0 (en) * 1998-05-20 2002-02-10 Chugai Pharmaceutical Co Ltd Novel method for gene cloning
WO2002023199A2 (en) 2000-09-14 2002-03-21 Reverse Proteomics Research Institute Co., Ltd. Method, system, apparatus and device for discovering and preparing chemical compounds
US20090137416A1 (en) 2001-01-16 2009-05-28 Regeneron Pharmaceuticals, Inc. Isolating Cells Expressing Secreted Proteins
PL369953A1 (en) * 2001-10-13 2005-05-02 Asterion Limited Glycosylphosphatidylinositol containing polypeptides
US20080020376A1 (en) * 2004-03-10 2008-01-24 Akopian Armen N Enriched Cdna Expression Libraries and Methods of Making and Using Same
WO2014006225A1 (en) * 2012-07-06 2014-01-09 Innovative Technologies In Biological Systems S.L. Fluorescent fusion polypeptide, biosensor comprising said polypeptide and uses thereof
TWI864338B (zh) 2012-11-14 2024-12-01 美商再生元醫藥公司 重組細胞表面捕捉蛋白質
WO2025252840A1 (en) * 2024-06-04 2025-12-11 Technische Universitaet Muenchen, In Vertretung Des Freistaates Bayern Half-life modulation by a tag comprising at least one constant domain of an immunoglobulin light chain

Family Cites Families (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5474899A (en) * 1987-05-13 1995-12-12 Cistron Biotechnology, Inc. Selective immunoassay for IL-1 β
US5223409A (en) 1988-09-02 1993-06-29 Protein Engineering Corp. Directed evolution of novel binding proteins
US5283173A (en) * 1990-01-24 1994-02-01 The Research Foundation Of State University Of New York System to detect protein-protein interactions
AU656934B2 (en) * 1990-11-23 1995-02-23 General Hospital Corporation, The Inhibition of cell adhesion protein-carbohydrate interactions
US6099842A (en) 1990-12-03 2000-08-08 The United States Of America As Represented By The Department Of Health And Human Services Recombinant immunotoxin composed of a single chain antibody reacting with the human transferrin receptor and diptheria toxin
US5571894A (en) * 1991-02-05 1996-11-05 Ciba-Geigy Corporation Recombinant antibodies specific for a growth factor receptor
EP0610286B1 (de) 1991-09-30 2000-03-15 THE UNITED STATES OF AMERICA, as represented by the Secretary of the Department of Health and Human Services Rekombinante immuntoxine
JP2879303B2 (ja) 1993-01-14 1999-04-05 佑 本庶 ｃＤＮＡライブラリーの作製方法、および新規なポリペプチドとそれをコードするＤＮＡ
US5817308A (en) * 1994-02-14 1998-10-06 University Of Rochester Tolerogenic fusion proteins of immunoglobulins and methods for inducing and maintaining tolerance
US5858752A (en) * 1995-06-07 1999-01-12 The General Hospital Corporation Fucosyltransferase genes and uses thereof
CA2223155C (en) 1995-06-07 2003-08-12 Zymogenetics, Inc. Secretion leader trap cloning method
WO1997040146A1 (en) 1996-04-24 1997-10-30 Genetics Institute, Inc. Yeast invertase gene as reporter system for isolating cytokines
JP2002238557A (ja) * 1996-07-23 2002-08-27 Fujisawa Pharmaceut Co Ltd トランスメンブレントラップ法
JPH1132779A (ja) 1997-07-24 1999-02-09 Fujita Gakuen 蛋白質の遺伝子クローニング法
US6030834A (en) * 1997-12-30 2000-02-29 Chiron Corporation Human IKK-beta DNA constructs and cells
JP2002513589A (ja) 1998-04-30 2002-05-14 マックス−プランク−ゲゼルシャフト・ツア・フェルデルング・デア・ヴィッセンシャフテン・エー・ファオ 再アレー化を伴う、発現ライブラリーのクローンを選択するための新規方法
IL139705A0 (en) 1998-05-20 2002-02-10 Chugai Pharmaceutical Co Ltd Novel method for gene cloning

Also Published As

Publication number Publication date
US7338797B2 (en) 2008-03-04
KR100518958B1 (ko) 2005-10-06
NO20005810D0 (no) 2000-11-17
EP1081224B1 (de) 2006-06-07
DE69931776D1 (de) 2006-07-20
JP3471006B2 (ja) 2003-11-25
CN1310758A (zh) 2001-08-29
KR20010043688A (ko) 2001-05-25
TW593679B (en) 2004-06-21
EP1081224A4 (de) 2003-07-02
US20050019780A1 (en) 2005-01-27
EP1081224A1 (de) 2001-03-07
AU753310B2 (en) 2002-10-17
CA2331917A1 (en) 1999-11-25
DE69931776T2 (de) 2007-05-16
AU3628399A (en) 1999-12-06
WO1999060113A1 (en) 1999-11-25
IL139705A0 (en) 2002-02-10
US6610485B1 (en) 2003-08-26
NO20005810L (no) 2001-01-22
US20030175794A1 (en) 2003-09-18

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