ATE334146T1 - METHOD FOR PRODUCING ERYTHROPOIETIN FREE OF ANIMAL PROTEINS - Google Patents

METHOD FOR PRODUCING ERYTHROPOIETIN FREE OF ANIMAL PROTEINS

Info

Publication number
ATE334146T1
ATE334146T1 AT03016548T AT03016548T ATE334146T1 AT E334146 T1 ATE334146 T1 AT E334146T1 AT 03016548 T AT03016548 T AT 03016548T AT 03016548 T AT03016548 T AT 03016548T AT E334146 T1 ATE334146 T1 AT E334146T1
Authority
AT
Austria
Prior art keywords
host cell
animal proteins
producing erythropoietin
protein
free
Prior art date
Application number
AT03016548T
Other languages
German (de)
Inventor
Josef Burg
Werner Fuerst
Walter Schneider
Karl-Heinz Sellinger
Alexander Wrba
Original Assignee
Roche Diagnostics Gmbh
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Roche Diagnostics Gmbh filed Critical Roche Diagnostics Gmbh
Application granted granted Critical
Publication of ATE334146T1 publication Critical patent/ATE334146T1/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/475—Growth factors; Growth regulators
    • C07K14/505—Erythropoietin [EPO]

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Toxicology (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

New therapeutic compsn. contains a protein (I) with erythropoietin activity produced in a host cell and: (a) contg. \~ 100 ppm protein from the host cell; (b) contg. \~ 10 pg DNA from the host cell per 83 ~mg (I), and (c) being entirely free of natural mammalian protein not derived from the host cell.
AT03016548T 1995-05-11 1996-05-10 METHOD FOR PRODUCING ERYTHROPOIETIN FREE OF ANIMAL PROTEINS ATE334146T1 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP95107165 1995-05-11
DE19522461 1995-06-21

Publications (1)

Publication Number Publication Date
ATE334146T1 true ATE334146T1 (en) 2006-08-15

Family

ID=26016131

Family Applications (2)

Application Number Title Priority Date Filing Date
AT96919753T ATE258189T1 (en) 1995-05-11 1996-05-10 METHOD FOR PRODUCING ERYTHROPOIETIN FREE OF ANIMAL PROTEINS
AT03016548T ATE334146T1 (en) 1995-05-11 1996-05-10 METHOD FOR PRODUCING ERYTHROPOIETIN FREE OF ANIMAL PROTEINS

Family Applications Before (1)

Application Number Title Priority Date Filing Date
AT96919753T ATE258189T1 (en) 1995-05-11 1996-05-10 METHOD FOR PRODUCING ERYTHROPOIETIN FREE OF ANIMAL PROTEINS

Country Status (12)

Country Link
US (2) US6399333B1 (en)
EP (2) EP1394179B1 (en)
JP (1) JP3061200B2 (en)
AT (2) ATE258189T1 (en)
AU (1) AU5817496A (en)
CA (1) CA2220515C (en)
DE (2) DE59611371D1 (en)
DK (2) DK0830376T3 (en)
ES (2) ES2268232T3 (en)
IL (2) IL118201A (en)
PT (2) PT1394179E (en)
WO (1) WO1996035718A1 (en)

Families Citing this family (38)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU729459B2 (en) * 1996-11-15 2001-02-01 Genentech Inc. Purification of neurotrophins
JP4541539B2 (en) * 1997-07-23 2010-09-08 ロシュ ダイアグノスティックス ゲーエムベーハー Production of erythropoietin by endogenous gene activation
US6548296B1 (en) * 1997-07-23 2003-04-15 Roche Diagnostics Gmbh Methods for identifying human cell lines useful for endogenous gene activation, isolated human lines identified thereby, and uses thereof
WO1999028346A1 (en) 1997-12-03 1999-06-10 Roche Diagnostics Gmbh Erythropoietin with high specific activity
NZ507557A (en) 1998-05-06 2003-10-31 Genentech Inc Protein purification by ion exchange chromatography
BR9917606A (en) * 1998-11-06 2002-12-31 Bio Sidus S A Procedure for the purification of recombinant human erythropoietin from cell culture supernatants and recombinant human erythropoietin obtained with such procedure
BR9905868A (en) 1998-11-06 2001-01-23 Bio Sidus S A Mass culture procedure for mammalian cells to obtain recombinant human erythropoietin and recombinant human erythropoietin obtained with such procedure
BR9905867A (en) * 1998-11-06 2001-01-23 Bio Sidus S A Recombinant human erythropoietin-producing cell line and the recombinant human erythropoietin produced by this cell
JO2291B1 (en) 1999-07-02 2005-09-12 اف . هوفمان لاروش ايه جي Erythopintin derivatives
AU2002233230B2 (en) 2000-12-20 2007-02-01 F. Hoffmann-La Roche Ag Erythropoietin conjugates
CN1596310A (en) * 2001-11-28 2005-03-16 桑多斯有限公司 Method for producing a recombinant polypeptide
ATE363541T1 (en) * 2002-03-26 2007-06-15 Lek Tovarna Farmacevtskih METHOD FOR PRODUCING A DESIRED PROFILE OF ERYTHROPOIETIN GLYCO ISOFORMS
DE60228460D1 (en) * 2002-12-13 2008-10-02 Bioceuticals Arzneimittel Ag Process for the preparation and purification of erythropoietin
US20050019914A1 (en) * 2003-07-24 2005-01-27 Aventis Pharma Deutschland Gmbh Perfusion process for producing erythropoietin
EP1670821A4 (en) * 2003-10-09 2006-11-29 Daewoong Co Ltd PROCESS FOR THE PURIFICATION OF HUMAN THROMBOPOIETIN WITH A HIGH SIALIC ACID CONTENT
DE102004027816A1 (en) * 2004-06-08 2006-01-05 Bioceuticals Arzneimittel Ag Process for the purification of erythropoietin
US7598356B2 (en) * 2004-07-08 2009-10-06 Board of Regents of the University of Nebraska by and on behalf of the University of Nebraska Medical Center Method for purifying a protein of the cystine-knot superfamily
DE602004013847D1 (en) * 2004-11-09 2008-06-26 Usv Ltd NEW PROCESS FOR CLEANING HUMAN GROWTH HORMONE
ES2509872T3 (en) * 2005-03-03 2014-10-20 Allergan, Inc. Procedures for obtaining a clostridic toxin
EP2495308A1 (en) 2005-12-08 2012-09-05 Amgen Inc. Improved production of glycoproteins using manganese
PL2054074T3 (en) 2006-08-04 2015-03-31 Prolong Pharmaceuticals Llc Modified erythropoietin
EP2089424B1 (en) * 2006-12-06 2015-07-01 JCR PHARMACEUTICALS Co., LTD. Method for production of human erythropoietin
KR100988706B1 (en) 2007-03-07 2010-10-18 한미홀딩스 주식회사 Novel Purification Method of Recombinant Erythropoietin
CL2008002053A1 (en) 2007-07-17 2009-05-22 Hoffmann La Roche Method for the purification of a monopeglated erythropoietin (epompeg) which consists of providing a solution containing mono, poly and non-peglated erythropoietin and passing it through two steps of cation exchange chromatography and a method to produce epo mpeg that includes a purification method.
AR067536A1 (en) * 2007-07-17 2009-10-14 Hoffmann La Roche METHOD FOR OBTAINING A MONO-PEGILATED ERYTHROPOYETIN IN A SUBSTANTIALLY HOMOGENOUS FORM
KR100900033B1 (en) 2007-11-21 2009-06-01 한국생명공학연구원 Method for Purifying Human Erythropoietin
MX344166B (en) 2008-02-08 2016-12-07 Ambrx Inc Modified leptin polypeptides and their uses.
DE102008002210A1 (en) * 2008-06-04 2009-12-10 Evonik Degussa Gmbh Process for the fermentative production of erythropoietin
DE102008002209A1 (en) 2008-06-04 2009-12-10 Evonik Degussa Gmbh Process for the purification of erythropoietin
CN102164954B (en) 2008-09-23 2018-04-06 弗·哈夫曼-拉罗切有限公司 The purifying of hematopoietin
BR122012024318A2 (en) 2008-09-26 2019-07-30 Ambrx, Inc. MODIFIED ANIMAL ERYTHROPOETIN POLYPEPTIDES AND THEIR USES
DE102008054716A1 (en) 2008-12-16 2010-06-17 Evonik Degussa Gmbh In-process control in a process for the production of EPO
US20120177640A1 (en) 2009-07-24 2012-07-12 Josef Burg Optimizing the production of antibodies
EA022396B1 (en) 2009-09-23 2015-12-30 Ратиофарм Гмбх Process for the purification of recombinant human erythropoietin (epo), epo thus purified and pharmaceutical compositions comprising same
KR101443257B1 (en) * 2011-10-18 2014-09-19 주식회사 종근당 Methods for Purifying Erythropoietin Analogs Having Lower Isoelectric Point
US10066001B2 (en) 2013-03-15 2018-09-04 Apotex Inc. Enhanced liquid formulation stability of erythropoietin alpha through purification processing
WO2016116966A1 (en) * 2015-01-22 2016-07-28 Jcr Pharmaceuticals Co., Ltd. Method for purification of recombinant human alpha-galactosidase a from material containing contaminant host cell proteins
DK3613486T3 (en) * 2018-08-24 2021-01-04 Uga Biopharma Gmbh METHOD AND SYSTEM FOR CLEANING EPO AND / OR AN EPO DERIVATIVE

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Publication number Priority date Publication date Assignee Title
NZ210501A (en) * 1983-12-13 1991-08-27 Kirin Amgen Inc Erythropoietin produced by procaryotic or eucaryotic expression of an exogenous dna sequence
US4677195A (en) * 1985-01-11 1987-06-30 Genetics Institute, Inc. Method for the purification of erythropoietin and erythropoietin compositions
IL79176A (en) * 1985-06-20 1992-06-21 Kirin Amgen Inc Process for the recovery of erythropoietin from a fluid
US4954437A (en) * 1986-09-15 1990-09-04 Integrated Genetics, Inc. Cell encoding recombinant human erythropoietin
AU4104889A (en) 1988-09-07 1990-03-15 Bioclones (Pty) Ltd Purification of erythropoietin
DE4115722A1 (en) * 1991-05-14 1992-11-19 Boehringer Mannheim Gmbh SERUM-FREE MEDIUM FOR CULTIVATING SUGAR CELLS

Also Published As

Publication number Publication date
IL118201A0 (en) 1996-09-12
DK0830376T3 (en) 2004-05-24
PT1394179E (en) 2006-11-30
DE59610898D1 (en) 2004-02-26
WO1996035718A1 (en) 1996-11-14
JP3061200B2 (en) 2000-07-10
JPH10506924A (en) 1998-07-07
ATE258189T1 (en) 2004-02-15
PT830376E (en) 2004-05-31
CA2220515C (en) 2003-07-22
EP1394179A1 (en) 2004-03-03
EP0830376B1 (en) 2004-01-21
US6471500B1 (en) 2002-10-29
ES2211961T3 (en) 2004-07-16
AU5817496A (en) 1996-11-29
IL157644A0 (en) 2004-03-28
DK1394179T3 (en) 2006-11-20
US20020146771A1 (en) 2002-10-10
IL118201A (en) 2004-12-15
EP1394179B1 (en) 2006-07-26
CA2220515A1 (en) 1996-11-14
DE59611371D1 (en) 2006-09-07
EP0830376A1 (en) 1998-03-25
ES2268232T3 (en) 2007-03-16
US6399333B1 (en) 2002-06-04

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