ATE349555T1 - Entdeckungs- und diagnoseverfahren mit 5- methylcytosin-dna-glycosylase - Google Patents

Entdeckungs- und diagnoseverfahren mit 5- methylcytosin-dna-glycosylase

Info

Publication number
ATE349555T1
ATE349555T1 AT03716589T AT03716589T ATE349555T1 AT E349555 T1 ATE349555 T1 AT E349555T1 AT 03716589 T AT03716589 T AT 03716589T AT 03716589 T AT03716589 T AT 03716589T AT E349555 T1 ATE349555 T1 AT E349555T1
Authority
AT
Austria
Prior art keywords
genomic dna
sequences
methods
present
methylated
Prior art date
Application number
AT03716589T
Other languages
English (en)
Inventor
Cathy E Lofton-Day
John K Day
Original Assignee
Epigenomics Ag
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Epigenomics Ag filed Critical Epigenomics Ag
Application granted granted Critical
Publication of ATE349555T1 publication Critical patent/ATE349555T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
AT03716589T 2002-03-15 2003-03-14 Entdeckungs- und diagnoseverfahren mit 5- methylcytosin-dna-glycosylase ATE349555T1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US36468902P 2002-03-15 2002-03-15

Publications (1)

Publication Number Publication Date
ATE349555T1 true ATE349555T1 (de) 2007-01-15

Family

ID=28041950

Family Applications (1)

Application Number Title Priority Date Filing Date
AT03716589T ATE349555T1 (de) 2002-03-15 2003-03-14 Entdeckungs- und diagnoseverfahren mit 5- methylcytosin-dna-glycosylase

Country Status (6)

Country Link
US (1) US7285394B2 (de)
EP (1) EP1487999B1 (de)
AT (1) ATE349555T1 (de)
AU (1) AU2003220291A1 (de)
DE (1) DE60310697D1 (de)
WO (1) WO2003078593A2 (de)

Families Citing this family (100)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7332275B2 (en) 1999-10-13 2008-02-19 Sequenom, Inc. Methods for detecting methylated nucleotides
US20110151438A9 (en) * 2001-11-19 2011-06-23 Affymetrix, Inc. Methods of Analysis of Methylation
WO2004011665A2 (en) * 2002-05-17 2004-02-05 Nugen Technologies, Inc. Methods for fragmentation, labeling and immobilization of nucleic acids
JP4384603B2 (ja) * 2002-06-26 2009-12-16 コールド スプリング ハーバー ラボラトリー メチル化プロファイルを測定するための方法および組成物
CN1774511B (zh) 2002-11-27 2013-08-21 斯昆诺有限公司 用于序列变异检测和发现的基于断裂的方法和系统
US20050009059A1 (en) * 2003-05-07 2005-01-13 Affymetrix, Inc. Analysis of methylation status using oligonucleotide arrays
WO2005024068A2 (en) 2003-09-05 2005-03-17 Sequenom, Inc. Allele-specific sequence variation analysis
EP1689887B1 (de) * 2003-10-21 2012-03-21 Orion Genomics, LLC Verfahren zur quantitativen bestimmung der methylierungsdichte in einem dna-locus
CA2552007A1 (en) * 2003-12-29 2005-07-21 Nugen Technologies, Inc. Methods for analysis of nucleic acid methylation status and methods for fragmentation, labeling and immobilization of nucleic acids
WO2005085477A1 (en) * 2004-03-02 2005-09-15 Orion Genomics Llc Differential enzymatic fragmentation by whole genome amplification
US7608394B2 (en) 2004-03-26 2009-10-27 Sequenom, Inc. Methods and compositions for phenotype identification based on nucleic acid methylation
EP1727911B1 (de) 2004-03-26 2013-01-23 Sequenom, Inc. Basenspezifische spaltung methylierungsspezifischer amplifikationsprodukte in kombination mit massenanalyse
DE102005023055A1 (de) * 2005-05-13 2006-11-16 Epigenomics Ag Verfahren zur Herstellung einer Mischung von Fragmenten einer Polynukleinsäure
US20060292585A1 (en) * 2005-06-24 2006-12-28 Affymetrix, Inc. Analysis of methylation using nucleic acid arrays
US7901882B2 (en) 2006-03-31 2011-03-08 Affymetrix, Inc. Analysis of methylation using nucleic acid arrays
EP3260556B1 (de) 2006-05-31 2019-07-31 Sequenom, Inc. Verfahren zur nukleinsäureextraktion aus einer probe
WO2008005459A2 (en) * 2006-06-30 2008-01-10 Nugen Technologies, Inc. Methods for fragmentation and labeling of nucleic acids
US7902345B2 (en) 2006-12-05 2011-03-08 Sequenom, Inc. Detection and quantification of biomolecules using mass spectrometry
CA2694820C (en) * 2007-07-30 2015-06-16 F. Hoffman-La Roche Ag Methods of identification using methylation of cpg
WO2009032781A2 (en) 2007-08-29 2009-03-12 Sequenom, Inc. Methods and compositions for universal size-specific polymerase chain reaction
US20090286286A1 (en) * 2007-11-06 2009-11-19 Ambergen , Inc. Methods for controlling amplification
US8486634B2 (en) 2007-11-06 2013-07-16 Ambergen, Inc. Amplifying bisulfite-treated template
US8932879B2 (en) * 2007-11-06 2015-01-13 Ambergen, Inc. Methods and compounds for phototransfer
US8906700B2 (en) 2007-11-06 2014-12-09 Ambergen, Inc. Methods and compositions for phototransfer
US8481263B2 (en) * 2007-11-06 2013-07-09 Ambergen Bead-ligand-nascent protein complexes
US20090270278A1 (en) * 2007-11-06 2009-10-29 Ambergen, Inc. Methods and compounds for making arrays
US20090264298A1 (en) * 2007-11-06 2009-10-22 Ambergen, Inc. Methods for enriching subpopulations
US20100075374A1 (en) * 2007-11-08 2010-03-25 Ambergen, Inc. Methods for capturing nascent proteins
US20090137024A1 (en) * 2007-11-28 2009-05-28 Canon U.S. Life Sciences, Inc. Method of Separating Target DNA from Mixed DNA
US7888127B2 (en) 2008-01-15 2011-02-15 Sequenom, Inc. Methods for reducing adduct formation for mass spectrometry analysis
EP4450642A3 (de) 2008-01-17 2025-01-08 Sequenom, Inc. Einzelmolekülnukleinsäurensequenzanalyseverfahren und zusammensetzungen
WO2009092035A2 (en) 2008-01-17 2009-07-23 Sequenom, Inc. Methods and compositions for the analysis of biological molecules
WO2009114543A2 (en) 2008-03-11 2009-09-17 Sequenom, Inc. Nucleic acid-based tests for prenatal gender determination
US8476013B2 (en) 2008-09-16 2013-07-02 Sequenom, Inc. Processes and compositions for methylation-based acid enrichment of fetal nucleic acid from a maternal sample useful for non-invasive prenatal diagnoses
US8962247B2 (en) 2008-09-16 2015-02-24 Sequenom, Inc. Processes and compositions for methylation-based enrichment of fetal nucleic acid from a maternal sample useful for non invasive prenatal diagnoses
EP2358911B1 (de) 2008-11-24 2016-12-21 Sequenom, Inc. Verbesserung von hybridisierungsverfahren durch kompression des dynamischen bereichs von molekülen die in hoher zahl vorhanden sind
US8771948B2 (en) 2009-04-03 2014-07-08 Sequenom, Inc. Nucleic acid preparation compositions and methods
DK2516680T3 (en) 2009-12-22 2016-05-02 Sequenom Inc Method and kits to identify aneuploidy
CA2831613A1 (en) 2011-03-31 2012-10-04 Moderna Therapeutics, Inc. Delivery and formulation of engineered nucleic acids
WO2012149339A2 (en) 2011-04-29 2012-11-01 Sequenom, Inc. Quantification of a minority nucleic acid species
WO2012177792A2 (en) 2011-06-24 2012-12-27 Sequenom, Inc. Methods and processes for non-invasive assessment of a genetic variation
US9984198B2 (en) 2011-10-06 2018-05-29 Sequenom, Inc. Reducing sequence read count error in assessment of complex genetic variations
WO2013052913A2 (en) 2011-10-06 2013-04-11 Sequenom, Inc. Methods and processes for non-invasive assessment of genetic variations
US10196681B2 (en) 2011-10-06 2019-02-05 Sequenom, Inc. Methods and processes for non-invasive assessment of genetic variations
EP2764458B1 (de) 2011-10-06 2021-04-07 Sequenom, Inc. Verfahren und prozesse zur nicht-invasiven beurteilung genetischer variationen
US10424394B2 (en) 2011-10-06 2019-09-24 Sequenom, Inc. Methods and processes for non-invasive assessment of genetic variations
US8688388B2 (en) 2011-10-11 2014-04-01 Sequenom, Inc. Methods and processes for non-invasive assessment of genetic variations
EP2769007B1 (de) 2011-10-19 2016-12-07 Nugen Technologies, Inc. Zusammensetzungen und verfahren zur direktionalen amplifikation und sequenzierung von nukleinsäuren
ES2929923T3 (es) 2012-01-20 2022-12-02 Sequenom Inc Procesos de diagnóstico que condicionan las condiciones experimentales
CN105861487B (zh) 2012-01-26 2020-05-05 纽亘技术公司 用于靶向核酸序列富集和高效文库产生的组合物和方法
EP4155401A1 (de) 2012-03-02 2023-03-29 Sequenom, Inc. Verfahren und prozesse zur nichtinvasiven beurteilung genetischer variationen
WO2013151664A1 (en) 2012-04-02 2013-10-10 modeRNA Therapeutics Modified polynucleotides for the production of proteins
US9283287B2 (en) 2012-04-02 2016-03-15 Moderna Therapeutics, Inc. Modified polynucleotides for the production of nuclear proteins
US9920361B2 (en) 2012-05-21 2018-03-20 Sequenom, Inc. Methods and compositions for analyzing nucleic acid
US10504613B2 (en) 2012-12-20 2019-12-10 Sequenom, Inc. Methods and processes for non-invasive assessment of genetic variations
CA2877094A1 (en) 2012-06-18 2013-12-27 Nugen Technologies, Inc. Compositions and methods for negative selection of non-desired nucleic acid sequences
US10497461B2 (en) 2012-06-22 2019-12-03 Sequenom, Inc. Methods and processes for non-invasive assessment of genetic variations
US20140004105A1 (en) 2012-06-29 2014-01-02 Sequenom, Inc. Age-related macular degeneration diagnostics
US20150011396A1 (en) 2012-07-09 2015-01-08 Benjamin G. Schroeder Methods for creating directional bisulfite-converted nucleic acid libraries for next generation sequencing
US20140093873A1 (en) 2012-07-13 2014-04-03 Sequenom, Inc. Processes and compositions for methylation-based enrichment of fetal nucleic acid from a maternal sample useful for non-invasive prenatal diagnoses
US10482994B2 (en) 2012-10-04 2019-11-19 Sequenom, Inc. Methods and processes for non-invasive assessment of genetic variations
US20130309666A1 (en) 2013-01-25 2013-11-21 Sequenom, Inc. Methods and processes for non-invasive assessment of genetic variations
US20130189684A1 (en) 2013-03-12 2013-07-25 Sequenom, Inc. Quantification of cell-specific nucleic acid markers
EP3597774A1 (de) 2013-03-13 2020-01-22 Sequenom, Inc. Primer zur analyse der dna-methylierung
US9305756B2 (en) 2013-03-13 2016-04-05 Agena Bioscience, Inc. Preparation enhancements and methods of use for MALDI mass spectrometry
EP4279610A3 (de) 2013-03-15 2024-01-03 ModernaTX, Inc. Ribonukleinsäurereinigung
EP2971033B8 (de) 2013-03-15 2019-07-10 ModernaTX, Inc. Herstellungsverfahren zur herstellung von rna-transkripten
WO2014144767A1 (en) 2013-03-15 2014-09-18 Moderna Therapeutics, Inc. Ion exchange purification of mrna
US10077439B2 (en) 2013-03-15 2018-09-18 Modernatx, Inc. Removal of DNA fragments in mRNA production process
EP2971130A4 (de) 2013-03-15 2016-10-05 Nugen Technologies Inc Sequentielle sequenzierung
EP4187543A1 (de) 2013-04-03 2023-05-31 Sequenom, Inc. Verfahren und prozesse zur nichtinvasiven beurteilung genetischer variationen
AU2014268377B2 (en) 2013-05-24 2020-10-08 Sequenom, Inc. Methods and processes for non-invasive assessment of genetic variations
DK3011051T3 (en) 2013-06-21 2019-04-23 Sequenom Inc Method for non-invasive evaluation of genetic variations
PL3019619T3 (pl) 2013-07-11 2022-01-10 Modernatx, Inc. Kompozycje zawierające syntetyczne polinkleotydy kodujące białka powiązane z crispr i syntetyczne sgrna oraz sposoby ich stosowania
EP3052511A4 (de) 2013-10-02 2017-05-31 Moderna Therapeutics, Inc. Polynukleotidmoleküle und verwendungen davon
US10964409B2 (en) 2013-10-04 2021-03-30 Sequenom, Inc. Methods and processes for non-invasive assessment of genetic variations
JP6680680B2 (ja) 2013-10-07 2020-04-15 セクエノム， インコーポレイテッド 染色体変化の非侵襲性評価のための方法およびプロセス
JP6525473B2 (ja) 2013-11-13 2019-06-05 ニューゲン テクノロジーズ， インコーポレイテッド 複製物配列決定リードを同定するための組成物および方法
WO2015131107A1 (en) 2014-02-28 2015-09-03 Nugen Technologies, Inc. Reduced representation bisulfite sequencing with diversity adaptors
WO2015138774A1 (en) 2014-03-13 2015-09-17 Sequenom, Inc. Methods and processes for non-invasive assessment of genetic variations
US10286086B2 (en) 2014-06-19 2019-05-14 Modernatx, Inc. Alternative nucleic acid molecules and uses thereof
US20170136132A1 (en) * 2014-06-19 2017-05-18 Moderna Therapeutics, Inc. Alternative nucleic acid molecules and uses thereof
AU2015289656A1 (en) 2014-07-16 2017-02-16 Modernatx, Inc. Circular polynucleotides
US11783911B2 (en) 2014-07-30 2023-10-10 Sequenom, Inc Methods and processes for non-invasive assessment of genetic variations
KR101654004B1 (ko) * 2014-10-30 2016-09-05 서울대학교산학협력단 애기장대 ap 엔도뉴클레아제를 이용한 dna 메틸화 부위의 형광 표지 방법
WO2016183289A1 (en) 2015-05-12 2016-11-17 Wake Forest University Health Services Identification of genetic modifications
EP4101930A1 (de) 2015-09-17 2022-12-14 ModernaTX, Inc. Polynukleotide mit stabilisierendem schwanzbereich
WO2017049286A1 (en) 2015-09-17 2017-03-23 Moderna Therapeutics, Inc. Polynucleotides containing a morpholino linker
US12385034B2 (en) 2016-06-24 2025-08-12 Modernatx, Inc. Methods and apparatus for filtration
US11200963B2 (en) 2016-07-27 2021-12-14 Sequenom, Inc. Genetic copy number alteration classifications
CA3207879C (en) 2017-01-24 2026-06-16 Sequenom, Inc. Methods and processes for assessment of genetic variations
EP3998350B1 (de) 2017-03-17 2025-05-07 Sequenom, Inc. Verfahren und vorgänge zur beurteilung von genetischem mosaizismus
US12492430B2 (en) 2017-04-11 2025-12-09 Tecan Genomics, Inc. Library quantitation and qualification
US11099202B2 (en) 2017-10-20 2021-08-24 Tecan Genomics, Inc. Reagent delivery system
EP3851542A1 (de) 2020-01-20 2021-07-21 Tecan Genomics, Inc. Verarmung von reichlich uninformativen sequenzen
CN113275053A (zh) 2020-02-03 2021-08-20 帝肯基因组学公司 试剂存储系统
CN116096915A (zh) * 2020-06-19 2023-05-09 格里尔公司 甲基化dna片段富集方法、组合物及套组
WO2023244983A1 (en) * 2022-06-13 2023-12-21 Freenome Holdings, Inc. Sequence process validation methods and compositions
US20250388955A1 (en) * 2022-06-30 2025-12-25 Illumina, Inc. Methylation detection with a non-natural/unnatural base
JP2026502527A (ja) * 2023-01-13 2026-01-23 エフ ホフマン－ラ ロッシュ アクチェン ゲゼルシャフト Ｄｎａ試料中の修飾核酸塩基の検出

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU5195398A (en) * 1996-10-25 1998-05-15 Advanced Research And Technology Institute, Inc. Dna sequences encoding fusions of dna repair proteins and uses thereof
US20040137456A1 (en) * 2002-04-04 2004-07-15 Hiroki Yokota Method for identifying and characterizing individual dna molecules

Also Published As

Publication number Publication date
EP1487999A4 (de) 2005-08-17
WO2003078593A2 (en) 2003-09-25
EP1487999A2 (de) 2004-12-22
US7285394B2 (en) 2007-10-23
US20030180779A1 (en) 2003-09-25
DE60310697D1 (de) 2007-02-08
AU2003220291A1 (en) 2003-09-29
WO2003078593A3 (en) 2004-07-29
EP1487999B1 (de) 2006-12-27
AU2003220291A8 (en) 2003-09-29

Similar Documents

Publication Publication Date Title
DE60310697D1 (de) Entdeckungs- und diagnoseverfahren mit 5-methylcytosin-dna-glycosylase
WO2003064701A3 (en) Method for the analysis of cytosine methylation patterns
DK0954608T3 (da) Methyleringsspecifik påvisning
BR0107574A (pt) Métodos e kits para identificar evento de elite gat-zm1 em amostras biológicas
WO2003027259A3 (en) Assays for dna methylation changes
WO2001057251A3 (en) Methods and apparatus for predicting, confirming, and displaying functional information derived from genomic sequence
AU2003291481A1 (en) Methods for identifying dna copy number changes
MXPA03003281A (es) Deteccion enzimatica no invasiva de estados asociados con la remodelacion de tejidos.
AU2001268449A1 (en) Toxicity typing using liver stem cells
CA2438574A1 (en) Method of detecting nucleotide polymorphism
AU2002252297A1 (en) Methods and tools for nucleic acid sequence analysis selection and generation
AU7486601A (en) Methods for assaying gene imprinting and methylated cpg islands
WO2001096604A3 (en) Assay for genetic polymorphisms using scattered light detectable labels
IL149791A0 (en) Oligonucleotides and assemblies thereof useful in the detection of the presence or absence of target nucleic acid sequences in a sample
WO2005012548A3 (en) Self-hybridizing multiple target nucleic acid probes and methods of use
WO2004062487A3 (en) Methods of detecting gene expression in normal and cancerous cells
ES2175095T3 (es) Marcadores de adn respecto al tamaño de una camada de cerdos.
AU2002303234A1 (en) Methods and apparatus for discovering, identifying and comparing biological activity mechanisms
DE602005020592D1 (de) Cpg-amplicon und array-protokoll
EP1258018A4 (de) Reagenzien, verfahren und kit zur erkennung von /i-proteinen des i-bazillus thuringiensis
WO2001009384A3 (en) Serial analysis of genetic alterations
AU2001286756A1 (en) Device for identifying the presence of a nucleotide sequence in a dna sample
AU2159801A (en) Test for micro-organisms
AU2003268634A1 (en) Culture having phenol oxidase-like activity
AU2003233219A1 (en) Ssh based methods for identifying and isolating unique nucleic acid sequences

Legal Events

Date Code Title Description
RER Ceased as to paragraph 5 lit. 3 law introducing patent treaties