ATE355367T1 - Normalisierte nukleinsäurebibliotheken sowie herstellungsverfahren derselben - Google Patents

Normalisierte nukleinsäurebibliotheken sowie herstellungsverfahren derselben

Info

Publication number
ATE355367T1
ATE355367T1 AT98948499T AT98948499T ATE355367T1 AT E355367 T1 ATE355367 T1 AT E355367T1 AT 98948499 T AT98948499 T AT 98948499T AT 98948499 T AT98948499 T AT 98948499T AT E355367 T1 ATE355367 T1 AT E355367T1
Authority
AT
Austria
Prior art keywords
nucleic acid
library
libraries
normalized
acid molecules
Prior art date
Application number
AT98948499T
Other languages
English (en)
Inventor
Wu-Bo Li
Joel Jessee
Paul E Nisson
Original Assignee
Invitrogen Corp
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Publication date
Application filed by Invitrogen Corp filed Critical Invitrogen Corp
Application granted granted Critical
Publication of ATE355367T1 publication Critical patent/ATE355367T1/de

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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034Isolating an individual clone by screening libraries
    • C12N15/1093General methods of preparing gene libraries, not provided for in other subgroups
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07HSUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1096Processes for the isolation, preparation or purification of DNA or RNA cDNA Synthesis; Subtracted cDNA library construction, e.g. RT, RT-PCR
    • CCHEMISTRY; METALLURGY
    • C40COMBINATORIAL TECHNOLOGY
    • C40BCOMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B30/00Methods of screening libraries
    • C40B30/06Methods of screening libraries by measuring effects on living organisms, tissues or cells
    • CCHEMISTRY; METALLURGY
    • C40COMBINATORIAL TECHNOLOGY
    • C40BCOMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B30/00Methods of screening libraries
    • C40B30/08Methods of screening libraries by measuring catalytic activity
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2563/00Nucleic acid detection characterized by the use of physical, structural and functional properties
    • C12Q2563/131Nucleic acid detection characterized by the use of physical, structural and functional properties the label being a member of a cognate binding pair, i.e. extends to antibodies, haptens, avidin
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00Oligonucleotides characterized by their use
    • C12Q2600/158Expression markers

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Plant Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • Microbiology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Saccharide Compounds (AREA)
AT98948499T 1997-09-24 1998-09-24 Normalisierte nukleinsäurebibliotheken sowie herstellungsverfahren derselben ATE355367T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US5981797P 1997-09-24 1997-09-24
US09/159,496 US6399334B1 (en) 1997-09-24 1998-09-23 Normalized nucleic acid libraries and methods of production thereof

Publications (1)

Publication Number Publication Date
ATE355367T1 true ATE355367T1 (de) 2006-03-15

Family

ID=26739222

Family Applications (1)

Application Number Title Priority Date Filing Date
AT98948499T ATE355367T1 (de) 1997-09-24 1998-09-24 Normalisierte nukleinsäurebibliotheken sowie herstellungsverfahren derselben

Country Status (9)

Country Link
US (4) US6399334B1 (de)
EP (1) EP1017853B1 (de)
JP (1) JP2001517460A (de)
AT (1) ATE355367T1 (de)
AU (1) AU9505998A (de)
CA (1) CA2304895A1 (de)
DE (1) DE69837208T2 (de)
ES (1) ES2281138T3 (de)
WO (1) WO1999015702A1 (de)

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US20090264304A1 (en) 2009-10-22
CA2304895A1 (en) 1999-04-01
EP1017853B1 (de) 2007-02-28
US20060269940A1 (en) 2006-11-30
EP1017853A4 (de) 2004-11-17
US20020034790A1 (en) 2002-03-21
WO1999015702A1 (en) 1999-04-01
US20020177155A1 (en) 2002-11-28
JP2001517460A (ja) 2001-10-09
AU9505998A (en) 1999-04-12
EP1017853A1 (de) 2000-07-12
US6399334B1 (en) 2002-06-04
DE69837208D1 (de) 2007-04-12
DE69837208T2 (de) 2007-11-22
ES2281138T3 (es) 2007-09-16

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