ATE357515T1 - Ligations-basierende synthese von oligonucleotiden mit blockstrukturen - Google Patents
Ligations-basierende synthese von oligonucleotiden mit blockstrukturenInfo
- Publication number
- ATE357515T1 ATE357515T1 AT04727252T AT04727252T ATE357515T1 AT E357515 T1 ATE357515 T1 AT E357515T1 AT 04727252 T AT04727252 T AT 04727252T AT 04727252 T AT04727252 T AT 04727252T AT E357515 T1 ATE357515 T1 AT E357515T1
- Authority
- AT
- Austria
- Prior art keywords
- oligo
- polynucleotides
- polynucleotide
- terminus
- nucleic acid
- Prior art date
Links
- 108091034117 Oligonucleotide Proteins 0.000 title 1
- JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 title 1
- 230000015572 biosynthetic process Effects 0.000 title 1
- 238000003786 synthesis reaction Methods 0.000 title 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/66—General methods for inserting a gene into a vector to form a recombinant vector using cleavage and ligation; Use of non-functional linkers or adaptors, e.g. linkers containing the sequence for a restriction endonuclease
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/26—Preparation of nitrogen-containing carbohydrates
- C12P19/28—N-glycosides
- C12P19/30—Nucleotides
- C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- General Health & Medical Sciences (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Plant Pathology (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Crystallography & Structural Chemistry (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP03008653 | 2003-04-15 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ATE357515T1 true ATE357515T1 (de) | 2007-04-15 |
Family
ID=33185845
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AT04727252T ATE357515T1 (de) | 2003-04-15 | 2004-04-14 | Ligations-basierende synthese von oligonucleotiden mit blockstrukturen |
Country Status (5)
| Country | Link |
|---|---|
| US (1) | US20090035823A1 (de) |
| EP (1) | EP1616008B1 (de) |
| AT (1) | ATE357515T1 (de) |
| DE (1) | DE602004005439D1 (de) |
| WO (1) | WO2004092375A2 (de) |
Families Citing this family (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2004029223A2 (en) * | 2002-09-30 | 2004-04-08 | Parallele Bioscience, Inc. | Polynucleotide synthesis and labeling by kinetic sampling ligation |
| EP2285974A2 (de) | 2008-05-14 | 2011-02-23 | British Columbia Cancer Agency Branch | Gensynthese durch konvergentes zusammenfügen von oligonukleotid-bauteilsätzen |
| CN104404134B (zh) * | 2009-04-03 | 2017-05-10 | 莱弗斯基因股份有限公司 | 多重核酸检测方法和系统 |
| US20130123121A1 (en) | 2010-11-22 | 2013-05-16 | The University Of Chicago | Methods and/or Use of Oligonucleotide-Bead Conjugates for Assays and Detections |
| GB2496016B (en) | 2011-09-09 | 2016-03-16 | Univ Leland Stanford Junior | Methods for obtaining a sequence |
| WO2013177046A1 (en) | 2012-05-21 | 2013-11-28 | Solulink, Inc. | Methods and/or use of oligonucleotide conjugates for suppressing background due to cross-hybridization |
| WO2015089053A1 (en) * | 2013-12-09 | 2015-06-18 | Integrated Dna Technologies, Inc. | Long nucleic acid sequences containing variable regions |
| EP2883963A1 (de) | 2013-12-16 | 2015-06-17 | Alacris Theranostics GmbH | Verfahren zur Erzeugung von Oligonukleotid-Arrays mit In-situ-Blocksyntheseansatz |
| US20180216253A1 (en) * | 2017-01-31 | 2018-08-02 | Counsyl, Inc. | Sequencing library preparation in small well format |
| AU2019207910A1 (en) * | 2018-01-12 | 2019-11-21 | Stem Arts Projects, Llc | Reaction condition composition for circularizing oligonucleotide probes |
Family Cites Families (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5114839A (en) * | 1988-05-24 | 1992-05-19 | Gesellschaft Fur Biotechnologische Forsching Mbh | Process for dna sequencing using oligonucleotide bank |
| US5851804A (en) * | 1996-05-06 | 1998-12-22 | Apollon, Inc. | Chimeric kanamycin resistance gene |
| US5942609A (en) * | 1998-11-12 | 1999-08-24 | The Porkin-Elmer Corporation | Ligation assembly and detection of polynucleotides on solid-support |
| EP1327682B1 (de) * | 2002-01-11 | 2009-05-13 | BioSpring Gesellschaft für Biotechnologie mbH | Verfahren zur Herstellung von DNA |
| EP1530581A4 (de) * | 2002-06-12 | 2006-08-30 | Datascope Investment Corp | Polymere markermoleküle |
-
2004
- 2004-04-14 US US10/553,104 patent/US20090035823A1/en not_active Abandoned
- 2004-04-14 EP EP04727252A patent/EP1616008B1/de not_active Expired - Lifetime
- 2004-04-14 WO PCT/EP2004/003921 patent/WO2004092375A2/en not_active Ceased
- 2004-04-14 AT AT04727252T patent/ATE357515T1/de not_active IP Right Cessation
- 2004-04-14 DE DE602004005439T patent/DE602004005439D1/de not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| WO2004092375A3 (en) | 2005-01-06 |
| WO2004092375A2 (en) | 2004-10-28 |
| US20090035823A1 (en) | 2009-02-05 |
| EP1616008B1 (de) | 2007-03-21 |
| DE602004005439D1 (de) | 2007-05-03 |
| EP1616008A2 (de) | 2006-01-18 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| RER | Ceased as to paragraph 5 lit. 3 law introducing patent treaties |