ATE358182T1 - Genomteilung - Google Patents
GenomteilungInfo
- Publication number
- ATE358182T1 ATE358182T1 AT03793900T AT03793900T ATE358182T1 AT E358182 T1 ATE358182 T1 AT E358182T1 AT 03793900 T AT03793900 T AT 03793900T AT 03793900 T AT03793900 T AT 03793900T AT E358182 T1 ATE358182 T1 AT E358182T1
- Authority
- AT
- Austria
- Prior art keywords
- nucleic acid
- layers
- fragments
- library
- sample nucleic
- Prior art date
Links
- 150000007523 nucleic acids Chemical class 0.000 abstract 6
- 102000039446 nucleic acids Human genes 0.000 abstract 5
- 108020004707 nucleic acids Proteins 0.000 abstract 5
- 239000012634 fragment Substances 0.000 abstract 4
- 238000000034 method Methods 0.000 abstract 4
- 238000005192 partition Methods 0.000 abstract 2
- 108091008146 restriction endonucleases Proteins 0.000 abstract 2
- 238000000638 solvent extraction Methods 0.000 abstract 2
- 239000013598 vector Substances 0.000 abstract 2
- 238000010276 construction Methods 0.000 abstract 1
- 238000012258 culturing Methods 0.000 abstract 1
- 238000003205 genotyping method Methods 0.000 abstract 1
- 238000004519 manufacturing process Methods 0.000 abstract 1
- 238000012216 screening Methods 0.000 abstract 1
- 230000001131 transforming effect Effects 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/66—General methods for inserting a gene into a vector to form a recombinant vector using cleavage and ligation; Use of non-functional linkers or adaptors, e.g. linkers containing the sequence for a restriction endonuclease
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
- C12N15/1089—Design, preparation, screening or analysis of libraries using computer algorithms
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
- C12N15/1093—General methods of preparing gene libraries, not provided for in other subgroups
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- General Engineering & Computer Science (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Organic Chemistry (AREA)
- Biomedical Technology (AREA)
- Wood Science & Technology (AREA)
- Biotechnology (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- Plant Pathology (AREA)
- Physics & Mathematics (AREA)
- Microbiology (AREA)
- Biophysics (AREA)
- General Health & Medical Sciences (AREA)
- Crystallography & Structural Chemistry (AREA)
- Bioinformatics & Computational Biology (AREA)
- Analytical Chemistry (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Saccharide Compounds (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GB0220649A GB0220649D0 (en) | 2002-09-05 | 2002-09-05 | Genome partitioning |
| GB0220773A GB0220773D0 (en) | 2002-09-06 | 2002-09-06 | Genome partitioning |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ATE358182T1 true ATE358182T1 (de) | 2007-04-15 |
Family
ID=31980014
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AT03793900T ATE358182T1 (de) | 2002-09-05 | 2003-09-05 | Genomteilung |
Country Status (7)
| Country | Link |
|---|---|
| US (1) | US20060281082A1 (de) |
| EP (1) | EP1546345B1 (de) |
| AT (1) | ATE358182T1 (de) |
| AU (1) | AU2003260790A1 (de) |
| CA (1) | CA2496517A1 (de) |
| DE (1) | DE60312875T2 (de) |
| WO (1) | WO2004022758A1 (de) |
Families Citing this family (31)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US8785353B2 (en) | 2005-06-23 | 2014-07-22 | Keygene N.V. | Strategies for high throughput identification and detection of polymorphisms |
| AU2006266251A1 (en) * | 2005-06-30 | 2007-01-11 | Syngenta Participations Ag | Methods for screening for gene specific hybridization polymorphisms (GSHPs) and their use in genetic mapping and marker development |
| WO2007028147A2 (en) * | 2005-09-01 | 2007-03-08 | Philadelphia Health & Education Corporation D.B.A. Drexel University College Of Medicin | Identification of a prostatic intraepithelial neoplasia (pin)-specific gene and protein (pin-1) useful as a diagnostic treatment for prostate cancer |
| US10316364B2 (en) | 2005-09-29 | 2019-06-11 | Keygene N.V. | Method for identifying the source of an amplicon |
| CA2910861C (en) | 2005-09-29 | 2018-08-07 | Michael Josephus Theresia Van Eijk | High throughput screening of mutagenized populations |
| ES2882401T3 (es) | 2005-12-22 | 2021-12-01 | Keygene Nv | Método de detección de polimorfismos basado en AFLP de alto rendimiento |
| EP2002017B1 (de) | 2006-04-04 | 2015-06-10 | Keygene N.V. | Nachweis von molekularmarkern mit hohem durchsatz auf der basis von restriktionsfragmenten |
| GB2487341A (en) * | 2009-11-02 | 2012-07-18 | Nugen Technologies Inc | Compositions and methods for targeted nucleic acid sequence selection and amplification |
| HK1199745A1 (en) * | 2011-09-09 | 2015-07-17 | The Board Of Trustees Of The Leland Stanford Junior University | Methods for obtaining a sequence |
| EP2769007B1 (de) | 2011-10-19 | 2016-12-07 | Nugen Technologies, Inc. | Zusammensetzungen und verfahren zur direktionalen amplifikation und sequenzierung von nukleinsäuren |
| CN104334739A (zh) | 2012-01-13 | 2015-02-04 | Data生物有限公司 | 通过新一代测序进行基因分型 |
| CN105861487B (zh) | 2012-01-26 | 2020-05-05 | 纽亘技术公司 | 用于靶向核酸序列富集和高效文库产生的组合物和方法 |
| CA2877094A1 (en) | 2012-06-18 | 2013-12-27 | Nugen Technologies, Inc. | Compositions and methods for negative selection of non-desired nucleic acid sequences |
| US20150011396A1 (en) | 2012-07-09 | 2015-01-08 | Benjamin G. Schroeder | Methods for creating directional bisulfite-converted nucleic acid libraries for next generation sequencing |
| EP2971130A4 (de) | 2013-03-15 | 2016-10-05 | Nugen Technologies Inc | Sequentielle sequenzierung |
| JP6525473B2 (ja) | 2013-11-13 | 2019-06-05 | ニューゲン テクノロジーズ， インコーポレイテッド | 複製物配列決定リードを同定するための組成物および方法 |
| WO2015131107A1 (en) | 2014-02-28 | 2015-09-03 | Nugen Technologies, Inc. | Reduced representation bisulfite sequencing with diversity adaptors |
| US12492430B2 (en) | 2017-04-11 | 2025-12-09 | Tecan Genomics, Inc. | Library quantitation and qualification |
| US11099202B2 (en) | 2017-10-20 | 2021-08-24 | Tecan Genomics, Inc. | Reagent delivery system |
| CN113166798B (zh) | 2018-11-28 | 2025-05-02 | 主基因有限公司 | 通过核酸内切酶保护的靶向富集 |
| EP3927840A1 (de) | 2019-02-21 | 2021-12-29 | Keygene N.V. | Genotypisierung von polyploiden |
| EP4073245A1 (de) | 2019-12-12 | 2022-10-19 | Keygene N.V. | Halbfestkörper-nukleinsäuremanipulation |
| JP7766029B2 (ja) | 2019-12-20 | 2025-11-07 | キージーン ナムローゼ フェンノートシャップ | 共有結合で閉端された核酸分子末端を使用したｎｇｓライブラリー調製 |
| EP3851542A1 (de) | 2020-01-20 | 2021-07-21 | Tecan Genomics, Inc. | Verarmung von reichlich uninformativen sequenzen |
| CN113275053A (zh) | 2020-02-03 | 2021-08-20 | 帝肯基因组学公司 | 试剂存储系统 |
| JP2023543602A (ja) | 2020-10-06 | 2023-10-17 | キージーン ナムローゼ フェンノートシャップ | 標的化された配列付加 |
| US20240002904A1 (en) | 2020-11-24 | 2024-01-04 | Keygene N.V. | Targeted enrichment using nanopore selective sequencing |
| US20240093288A1 (en) | 2020-11-25 | 2024-03-21 | Koninklijke Nederlandse Akademie Van Wetenschappen | Ribosomal profiling in single cells |
| WO2024121354A1 (en) | 2022-12-08 | 2024-06-13 | Keygene N.V. | Duplex sequencing with covalently closed dna ends |
| CN116286991B (zh) * | 2023-02-10 | 2023-10-13 | 中国农业科学院农业基因组研究所 | 全基因组增强子筛选系统、筛选方法及应用 |
| WO2024209000A1 (en) | 2023-04-04 | 2024-10-10 | Keygene N.V. | Linkers for duplex sequencing |
Family Cites Families (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1995011995A1 (en) * | 1993-10-26 | 1995-05-04 | Affymax Technologies N.V. | Arrays of nucleic acid probes on biological chips |
| JP3954092B2 (ja) * | 1993-06-25 | 2007-08-08 | アフィメトリックス インコーポレイテッド | 核酸配列のハイブリダイゼーションと配列決定 |
| WO1998051789A2 (en) * | 1997-05-13 | 1998-11-19 | Display Systems Biotech A/S | A METHOD TO CLONE mRNAs AND DISPLAY OF DIFFERENTIALLY EXPRESSED TRANSCRIPTS (DODET) |
| EP1001037A3 (de) * | 1998-09-28 | 2003-10-01 | Whitehead Institute For Biomedical Research | Vorselektion und Isolierung eines Einzelnukleotidpolymorphismus |
| US20020090612A1 (en) * | 1999-01-08 | 2002-07-11 | Jonathan M. Rothberg | Method of identifying nucleic acids |
| EP1196554B1 (de) * | 1999-03-18 | 2009-02-25 | Complete Genomics AS | Methoden zur klonierung und herstelung von kettenfragmenten mit lesbarem inhalt an information |
-
2003
- 2003-09-05 CA CA002496517A patent/CA2496517A1/en not_active Abandoned
- 2003-09-05 DE DE60312875T patent/DE60312875T2/de not_active Expired - Fee Related
- 2003-09-05 US US10/526,571 patent/US20060281082A1/en not_active Abandoned
- 2003-09-05 EP EP03793900A patent/EP1546345B1/de not_active Expired - Lifetime
- 2003-09-05 AT AT03793900T patent/ATE358182T1/de not_active IP Right Cessation
- 2003-09-05 WO PCT/GB2003/003866 patent/WO2004022758A1/en not_active Ceased
- 2003-09-05 AU AU2003260790A patent/AU2003260790A1/en not_active Abandoned
Also Published As
| Publication number | Publication date |
|---|---|
| AU2003260790A1 (en) | 2004-03-29 |
| US20060281082A1 (en) | 2006-12-14 |
| CA2496517A1 (en) | 2004-03-18 |
| EP1546345B1 (de) | 2007-03-28 |
| DE60312875T2 (de) | 2007-12-06 |
| WO2004022758A1 (en) | 2004-03-18 |
| DE60312875D1 (de) | 2007-05-10 |
| EP1546345A1 (de) | 2005-06-29 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| RER | Ceased as to paragraph 5 lit. 3 law introducing patent treaties |