ATE360063T1 - Nicht störendes, dreidimensionales system für die kultivierung und ernte verankerungsabhängiger zellen - Google Patents
Nicht störendes, dreidimensionales system für die kultivierung und ernte verankerungsabhängiger zellenInfo
- Publication number
- ATE360063T1 ATE360063T1 AT01981506T AT01981506T ATE360063T1 AT E360063 T1 ATE360063 T1 AT E360063T1 AT 01981506 T AT01981506 T AT 01981506T AT 01981506 T AT01981506 T AT 01981506T AT E360063 T1 ATE360063 T1 AT E360063T1
- Authority
- AT
- Austria
- Prior art keywords
- interruptional
- anchorage
- harvesting
- cultivation
- dimensional system
- Prior art date
Links
- 238000003306 harvesting Methods 0.000 title 1
- 102000010834 Extracellular Matrix Proteins Human genes 0.000 abstract 2
- 108010037362 Extracellular Matrix Proteins Proteins 0.000 abstract 2
- 210000002744 extracellular matrix Anatomy 0.000 abstract 2
- 229920001222 biopolymer Polymers 0.000 abstract 1
- 230000003915 cell function Effects 0.000 abstract 1
- 239000003094 microcapsule Substances 0.000 abstract 1
- 229920000642 polymer Polymers 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/0062—General methods for three-dimensional culture
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/0012—Cell encapsulation
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/067—Hepatocytes
- C12N5/0671—Three-dimensional culture, tissue culture or organ culture; Encapsulated cells
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
- A61K2035/126—Immunoprotecting barriers, e.g. jackets, diffusion chambers
- A61K2035/128—Immunoprotecting barriers, e.g. jackets, diffusion chambers capsules, e.g. microcapsules
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2533/00—Supports or coatings for cell culture, characterised by material
- C12N2533/30—Synthetic polymers
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2533/00—Supports or coatings for cell culture, characterised by material
- C12N2533/50—Proteins
- C12N2533/54—Collagen; Gelatin
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2533/00—Supports or coatings for cell culture, characterised by material
- C12N2533/70—Polysaccharides
- C12N2533/72—Chitin, chitosan
Landscapes
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Life Sciences & Earth Sciences (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- Organic Chemistry (AREA)
- Chemical & Material Sciences (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Cell Biology (AREA)
- Gastroenterology & Hepatology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Materials For Medical Uses (AREA)
- Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
- Prostheses (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US23925900P | 2000-10-12 | 2000-10-12 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ATE360063T1 true ATE360063T1 (de) | 2007-05-15 |
Family
ID=22901354
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AT01981506T ATE360063T1 (de) | 2000-10-12 | 2001-10-12 | Nicht störendes, dreidimensionales system für die kultivierung und ernte verankerungsabhängiger zellen |
Country Status (6)
| Country | Link |
|---|---|
| US (4) | US6905875B2 (de) |
| EP (1) | EP1326968B1 (de) |
| AT (1) | ATE360063T1 (de) |
| AU (1) | AU2002213143A1 (de) |
| DE (1) | DE60127983T2 (de) |
| WO (1) | WO2002031135A1 (de) |
Families Citing this family (79)
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| WO2004069169A2 (en) | 2003-01-31 | 2004-08-19 | Scimed Life Systems, Inc. | Localized drug delivery using drug-loaded nanocapsules and implantable device coated with the same |
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| US7927599B2 (en) | 2003-09-08 | 2011-04-19 | Ethicon, Inc. | Chondrocyte therapeutic delivery system |
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| US20050272153A1 (en) | 2004-01-27 | 2005-12-08 | Zou Xuenong | Bone tissue engineering by ex vivo stem cells ongrowth into three-dimensional trabecular metal |
| US20060134779A1 (en) * | 2004-03-10 | 2006-06-22 | Banes Albert J | Modulation of cell intrinsic strain to control cell modulus, matrix synthesis, secretion, organization, material properties and remodeling of tissue engineered constructs |
| US7700333B2 (en) * | 2004-07-26 | 2010-04-20 | Agency For Science Technology & Research | Immobilization of cells in a matrix formed by biocompatible charged polymers under laminar flow conditions |
| US7704714B2 (en) | 2004-07-26 | 2010-04-27 | Agency For Science, Technology & Research | Encapsulation of cells in biologic compatible scaffolds by coacervation of charged polymers |
| JP2008519598A (ja) * | 2004-11-11 | 2008-06-12 | エージェンシー フォー サイエンス,テクノロジー アンド リサーチ | 細胞培養デバイス |
| US7851189B2 (en) | 2005-03-07 | 2010-12-14 | Boston Scientific Scimed, Inc. | Microencapsulated compositions for endoluminal tissue engineering |
| US7674864B2 (en) * | 2005-12-23 | 2010-03-09 | Boston Scientific Scimed, Inc. | Polymeric hybrid precursors, polymeric hybrid precursor composite matrices, medical devices, and methods |
| US8455088B2 (en) | 2005-12-23 | 2013-06-04 | Boston Scientific Scimed, Inc. | Spun nanofiber, medical devices, and methods |
| AU2007212085B2 (en) * | 2006-02-07 | 2011-11-17 | Spinalcyte, Llc | Methods and compositions for repair of cartilage using an in vivo bioreactor |
| ES2549528T3 (es) * | 2006-03-23 | 2015-10-29 | Pluristem Ltd. | Métodos de expansión de células y usos de células y de medios de acondicionamiento producidos por los mismos para terapia |
| US20070237749A1 (en) * | 2006-04-07 | 2007-10-11 | Wang Taylor G | Multi-membrane immunoisolation system for cellular transplant |
| US20070238169A1 (en) * | 2006-04-11 | 2007-10-11 | The Board Of Trustees Of The Leland Stanford Junior University | Cell sorter and culture system |
| US20090263849A1 (en) * | 2006-04-21 | 2009-10-22 | Drexel University | Bioprinting Three-Dimensional Structure Onto Microscale Tissue Analog Devices for Pharmacokinetic Study and Other Uses |
| WO2007127231A2 (en) * | 2006-04-24 | 2007-11-08 | The Johns Hopkins University | Magnetic resonance-detectable, ultrasound-detectable and/or radiopaque microcapsules and uses thereof |
| US8679809B2 (en) * | 2006-05-19 | 2014-03-25 | The University Of Hong Kong | Cell-matrix microspheres, methods for preparation and applications |
| ATE552867T1 (de) * | 2006-06-16 | 2012-04-15 | Fmc Biopolymer As | Alginatbeschichtete collagenmatrix- zellvorrichtung, herstellungsverfahren dafür und verwendungen davon |
| US8206816B2 (en) * | 2006-08-29 | 2012-06-26 | Florida State University Research Foundation, Inc. | Polymer mechanical damping composites and methods of production |
| US8449981B2 (en) * | 2006-12-13 | 2013-05-28 | Basf Se | Microcapsules |
| WO2008130529A1 (en) * | 2007-04-16 | 2008-10-30 | University Of Toledo | Hybrid biomimetic particles, methods of making same and uses therefor |
| US20080281419A1 (en) * | 2007-05-10 | 2008-11-13 | Matheny Robert G | Breast implants and compositions of extracellular matrix |
| EP2173858A4 (de) * | 2007-07-02 | 2010-09-08 | Univ Columbia | Verarbeitung von biologisch abgeleitetem verbundgewebe |
| KR20100049084A (ko) | 2007-08-23 | 2010-05-11 | 인트렉손 코포레이션 | 질병 진단을 위한 방법 및 조성물 |
| US20100278798A1 (en) * | 2007-09-27 | 2010-11-04 | The Trustees Of Columbia University In The City Of New York | Methods and systems for forming biocompatible materials |
| KR101666228B1 (ko) | 2007-09-28 | 2016-10-13 | 인트렉손 코포레이션 | 생물치료학적 분자를 발현시키기 위한 치료학적 유전자-스위치 작제물 및 생물반응기, 및 이의 용도 |
| EP2203548A4 (de) * | 2007-10-11 | 2014-01-15 | Agency Science Tech & Res | Bildung einer zellstruktur mit transientem linker in einem käfig |
| US20090137925A1 (en) * | 2007-11-23 | 2009-05-28 | Divya Cantor | Impedance Spectroscopy Cervix Scanning Apparatus and Method |
| US8613776B2 (en) * | 2007-12-27 | 2013-12-24 | The Trustees Of Columbia University In The City Of New York | Systems and methods for forming patterned extracellular matrix materials |
| US8114918B2 (en) | 2008-08-15 | 2012-02-14 | The Florida State University Research Foundation, Inc. | Compacted polyelectrolyte complexes and articles |
| US20100166822A1 (en) * | 2008-12-31 | 2010-07-01 | Howmedica Osteonics Corp. | Adhesive cartilage implant |
| US20110136162A1 (en) * | 2009-08-31 | 2011-06-09 | Drexel University | Compositions and Methods for Functionalized Patterning of Tissue Engineering Substrates Including Bioprinting Cell-Laden Constructs for Multicompartment Tissue Chambers |
| WO2012048275A2 (en) | 2010-10-08 | 2012-04-12 | Caridianbct, Inc. | Configurable methods and systems of growing and harvesting cells in a hollow fiber bioreactor system |
| EP3260533B1 (de) | 2011-03-22 | 2019-09-11 | Pluristem Ltd. | Verfahren zur behandlung von strahlungs- oder chemischen verletzungen |
| US10016461B2 (en) | 2012-12-03 | 2018-07-10 | The Regents Of The University Of California | Apparatus and process for growing a heart valve in three-dimensions |
| US9968446B2 (en) | 2011-03-23 | 2018-05-15 | The Regents Of The University Of California | Tubular scaffold for fabrication of heart valves |
| US10085946B2 (en) * | 2012-09-04 | 2018-10-02 | Technion Research & Development Foundation Limited | Use of decellularized extracellular matrix for encapsulating cells |
| TWI512103B (zh) | 2012-11-12 | 2015-12-11 | Ind Tech Res Inst | 細胞培養系統、以及無血清細胞培養方法 |
| US20160053231A1 (en) * | 2013-04-10 | 2016-02-25 | Tufts University | Two and three dimensional decellularized ecm constructs and uses therefor |
| KR102232650B1 (ko) | 2013-06-11 | 2021-03-29 | 프레지던트 앤드 펠로우즈 오브 하바드 칼리지 | SC-β 세포 및 조성물 그리고 그 생성 방법 |
| WO2014204806A2 (en) | 2013-06-19 | 2014-12-24 | Spinalcyte, Llc | Adipose cells for chondrocyte applications |
| CN105793411B (zh) | 2013-11-16 | 2018-04-17 | 泰尔茂比司特公司 | 生物反应器中的细胞扩增 |
| US10253203B2 (en) | 2014-02-04 | 2019-04-09 | The Florida State University Research Foundation, Inc. | Rough polyelectrolyte complex coatings and methods of forming |
| JP6783143B2 (ja) | 2014-03-25 | 2020-11-11 | テルモ ビーシーティー、インコーポレーテッド | 培地の受動的補充 |
| US20170287540A1 (en) * | 2014-09-25 | 2017-10-05 | Hewlett Packard Enterprise Development Lp | Crosspoint array decoder |
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| US9919280B2 (en) | 2014-11-24 | 2018-03-20 | The Florida State University Research Foundation, Inc. | Method of forming polyelectrolyte complex capsules |
| CN107614678B (zh) | 2014-12-18 | 2021-04-30 | 哈佛学院校长同事会 | 干细胞来源的β细胞的产生方法及其使用方法 |
| ES2985135T3 (es) | 2014-12-18 | 2024-11-04 | Harvard College | Procedimientos para generar células ß derivadas de células madre y usos de las mismas |
| WO2016100898A1 (en) | 2014-12-18 | 2016-06-23 | President And Fellows Of Harvard College | Serum-free in vitro directed differentiation protocol for generating stem cell-derived b cells and uses thereof |
| US20160175363A1 (en) * | 2014-12-18 | 2016-06-23 | President And Fellows Of Harvard College | Methods for generating stem cell-derived beta cells and uses thereof |
| US10647953B2 (en) | 2015-02-11 | 2020-05-12 | The Florida State University Research Foundation, Inc. | Surface treatment for cell culture |
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| CA2998991A1 (en) | 2015-09-25 | 2017-04-20 | Maxivax Sa | Vaccination with immuno-isolated cells producing an immunomodulator |
| US20190060367A1 (en) * | 2016-03-03 | 2019-02-28 | Henry Ford Health System | 3-d collagen scaffold-generated exosomes and uses thereof |
| US11965175B2 (en) | 2016-05-25 | 2024-04-23 | Terumo Bct, Inc. | Cell expansion |
| US11685883B2 (en) | 2016-06-07 | 2023-06-27 | Terumo Bct, Inc. | Methods and systems for coating a cell growth surface |
| US11104874B2 (en) | 2016-06-07 | 2021-08-31 | Terumo Bct, Inc. | Coating a bioreactor |
| TR201611332A2 (tr) | 2016-08-11 | 2018-02-21 | Mehmet Dogan Asik | Bi̇r mi̇krokapsül ve üreti̇m yöntemi̇ |
| US10328535B2 (en) * | 2016-11-07 | 2019-06-25 | International Business Machines Corporation | Self-heating solder flux material |
| US12234441B2 (en) | 2017-03-31 | 2025-02-25 | Terumo Bct, Inc. | Cell expansion |
| US11629332B2 (en) | 2017-03-31 | 2023-04-18 | Terumo Bct, Inc. | Cell expansion |
| US11624046B2 (en) | 2017-03-31 | 2023-04-11 | Terumo Bct, Inc. | Cell expansion |
| US11964250B2 (en) * | 2017-08-15 | 2024-04-23 | University Of Maryland, College Park | Multicompartment capsules and methods and systems for forming same |
| AU2018370029B2 (en) | 2017-11-15 | 2024-11-07 | Vertex Pharmaceuticals Incorporated | Islet cell manufacturing compositions and methods of use |
| WO2019136021A1 (en) * | 2018-01-03 | 2019-07-11 | Sakkos Jonathan Konstantine | Biological assembly including biological component and shield |
| CN108251410A (zh) * | 2018-01-24 | 2018-07-06 | 南京大学 | 一种包埋气凝胶的固定化微生物制备方法 |
| WO2020033879A1 (en) | 2018-08-10 | 2020-02-13 | Semma Therapeutics, Inc. | Stem cell derived islet differentiation |
| US11518971B2 (en) | 2018-11-27 | 2022-12-06 | Research Triangle Institute | Method and apparatus for spatial control of cellular growth |
| CN111621493B (zh) * | 2020-06-10 | 2022-02-15 | 航天中心医院 | 可裂解细胞微囊及其制备方法和细胞培养方法 |
| EP4314244B1 (de) | 2021-03-23 | 2025-07-23 | Terumo BCT, Inc. | Zellerfassung und -erweiterung |
| CN114177279B (zh) * | 2021-12-23 | 2024-03-26 | 广西信业生物技术有限公司 | 一种纳米微囊结构的医用眼贴及制备方法 |
| US12152699B2 (en) | 2022-02-28 | 2024-11-26 | Terumo Bct, Inc. | Multiple-tube pinch valve assembly |
| USD1099116S1 (en) | 2022-09-01 | 2025-10-21 | Terumo Bct, Inc. | Display screen or portion thereof with a graphical user interface for displaying cell culture process steps and measurements of an associated bioreactor device |
Family Cites Families (19)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3959078A (en) | 1973-05-18 | 1976-05-25 | Midwest Research Institute | Enzyme immobilization with a thermochemical-photochemical bifunctional agent |
| US4293654A (en) | 1977-10-17 | 1981-10-06 | Massachusetts Institute Of Technology | Cell culture microcarriers |
| US4743545A (en) * | 1984-08-09 | 1988-05-10 | Torobin Leonard B | Hollow porous microspheres containing biocatalyst |
| US4798786A (en) | 1982-05-06 | 1989-01-17 | Stolle Research And Development Corporation | Living cells encapsulated in crosslinked protein |
| SE464816B (sv) | 1985-10-15 | 1991-06-17 | Nilsson Kjell | Makroporoesa partiklar, foerfarande foer dess framstaellning och dess anvaendning |
| US4994388A (en) | 1988-04-15 | 1991-02-19 | Solohill Engineering, Inc. | Collagen-coated polystyrene microcarrier beads |
| AU7787191A (en) | 1989-11-09 | 1991-06-13 | Bio-Metric Systems, Inc. | Improved bioreactor surfaces and methods of making same |
| EP0529751A1 (de) * | 1991-08-09 | 1993-03-03 | W.R. Grace & Co.-Conn. | Zellkulturträger und -testmaterial |
| US5908623A (en) * | 1993-08-12 | 1999-06-01 | Cytotherapeutics, Inc. | Compositions and methods for the delivery of biologically active molecules using genetically altered cells contained in biocompatible immunoisolatory capsules |
| US5846952A (en) | 1993-12-01 | 1998-12-08 | Marine Polymer Technologies, Inc. | Methods and compositions for poly-β-1-4-N-acetylglucosamine drug delivery |
| US5858350A (en) | 1993-12-01 | 1999-01-12 | Marine Polymer Technologies | Methods and compositions for poly-β-1→4-N-acetylglucosamine cell therapy system |
| US5620883A (en) * | 1994-04-01 | 1997-04-15 | The Johns Hopkins University | Living cells microencapsulated in a polymeric membrane having two layers |
| FR2721516B1 (fr) * | 1994-06-27 | 1996-09-13 | Inst Rech Biolog Sa | Nouvelles utilisations d'un complexe à base de phospholipides cérébraux en thérapeutique et dans l'alimentation. |
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| US7160724B2 (en) * | 2000-03-09 | 2007-01-09 | University Of South Florida | Human cord blood as a source of neural tissue for repair of the brain and spinal cord |
| US20010049139A1 (en) * | 2000-03-23 | 2001-12-06 | Eric Lagasse | Hepatic regeneration from hematopoietic stem cells |
| US20040136973A1 (en) * | 2002-11-07 | 2004-07-15 | Eliezer Huberman | Human stem cell materials and methods |
-
2001
- 2001-10-12 AT AT01981506T patent/ATE360063T1/de not_active IP Right Cessation
- 2001-10-12 US US10/398,957 patent/US6905875B2/en not_active Expired - Fee Related
- 2001-10-12 EP EP01981506A patent/EP1326968B1/de not_active Expired - Lifetime
- 2001-10-12 AU AU2002213143A patent/AU2002213143A1/en not_active Abandoned
- 2001-10-12 WO PCT/US2001/031890 patent/WO2002031135A1/en not_active Ceased
- 2001-10-12 US US09/975,273 patent/US6916640B2/en not_active Expired - Fee Related
- 2001-10-12 DE DE60127983T patent/DE60127983T2/de not_active Expired - Lifetime
-
2005
- 2005-05-26 US US11/137,483 patent/US20050220891A1/en not_active Abandoned
-
2009
- 2009-07-23 US US12/507,900 patent/US7943353B2/en not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| EP1326968A4 (de) | 2004-10-06 |
| US20020094569A1 (en) | 2002-07-18 |
| US20050220891A1 (en) | 2005-10-06 |
| US6905875B2 (en) | 2005-06-14 |
| EP1326968A1 (de) | 2003-07-16 |
| DE60127983D1 (de) | 2007-05-31 |
| US20040023370A1 (en) | 2004-02-05 |
| US20090286278A1 (en) | 2009-11-19 |
| US7943353B2 (en) | 2011-05-17 |
| US6916640B2 (en) | 2005-07-12 |
| WO2002031135A1 (en) | 2002-04-18 |
| EP1326968B1 (de) | 2007-04-18 |
| AU2002213143A1 (en) | 2002-04-22 |
| DE60127983T2 (de) | 2008-01-17 |
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