ATE361308T1 - Verdrängungsreagenzien mit niedrigem molekulargewicht und hoher affinität zur reinigung von oligonukleotiden - Google Patents

Verdrängungsreagenzien mit niedrigem molekulargewicht und hoher affinität zur reinigung von oligonukleotiden

Info

Publication number
ATE361308T1
ATE361308T1 AT00943241T AT00943241T ATE361308T1 AT E361308 T1 ATE361308 T1 AT E361308T1 AT 00943241 T AT00943241 T AT 00943241T AT 00943241 T AT00943241 T AT 00943241T AT E361308 T1 ATE361308 T1 AT E361308T1
Authority
AT
Austria
Prior art keywords
molecular weight
low molecular
high affinity
oligonucleotides
purifying oligonucleotides
Prior art date
Application number
AT00943241T
Other languages
English (en)
Inventor
Abhinav A Shukla
Ranjit R Deshmukh
Steven M Cramer
James A Moore
Original Assignee
Isis Pharmaceuticals Inc
Rensselaer Polytech Inst
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Isis Pharmaceuticals Inc, Rensselaer Polytech Inst filed Critical Isis Pharmaceuticals Inc
Application granted granted Critical
Publication of ATE361308T1 publication Critical patent/ATE361308T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • C12N15/101—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by chromatography, e.g. electrophoresis, ion-exchange, reverse phase
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/42—Selective adsorption, e.g. chromatography characterised by the development mode, e.g. by displacement or by elution
    • B01D15/422—Displacement mode

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Saccharide Compounds (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Detergent Compositions (AREA)
AT00943241T 1999-06-29 2000-06-28 Verdrängungsreagenzien mit niedrigem molekulargewicht und hoher affinität zur reinigung von oligonukleotiden ATE361308T1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US09/343,006 US6573373B1 (en) 1999-06-29 1999-06-29 High affinity, low molecular weight displacers for oligonucleotide purification

Publications (1)

Publication Number Publication Date
ATE361308T1 true ATE361308T1 (de) 2007-05-15

Family

ID=23344283

Family Applications (1)

Application Number Title Priority Date Filing Date
AT00943241T ATE361308T1 (de) 1999-06-29 2000-06-28 Verdrängungsreagenzien mit niedrigem molekulargewicht und hoher affinität zur reinigung von oligonukleotiden

Country Status (7)

Country Link
US (2) US6573373B1 (de)
EP (1) EP1192172B1 (de)
JP (1) JP2003503418A (de)
AT (1) ATE361308T1 (de)
AU (1) AU5774000A (de)
DE (1) DE60034676T2 (de)
WO (1) WO2001000642A1 (de)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6573373B1 (en) * 1999-06-29 2003-06-03 Isis Pharmaceuticals, Inc. High affinity, low molecular weight displacers for oligonucleotide purification
AU2003213642A1 (en) 2002-02-28 2003-09-16 Rensselaer Polytechnic Institute High-affinity, low-molecular-mass displacers for ion-exchange chromatography
US8080560B2 (en) * 2004-12-17 2011-12-20 3M Innovative Properties Company Immune response modifier formulations containing oleic acid and methods
WO2006116064A2 (en) * 2005-04-22 2006-11-02 Waters Investments Limited Apparatus and methods for mass-spectrometric directed purification of biopolymers
US7994220B2 (en) 2005-09-28 2011-08-09 Cypress Bioscience, Inc. Milnacipran for the long-term treatment of fibromyalgia syndrome
KR101408026B1 (ko) 2005-12-02 2014-06-20 사켐,인코포레이티드 음이온-교환 치환 크로마토그래피 공정 및 음이온-교환치환 크로마토그래피 공정에서 디스플레이서 화합물들로서사용하기 위한 음이온성 유기 화합물들
GB201115218D0 (en) * 2011-09-02 2011-10-19 Glaxo Group Ltd Novel process
TWI432499B (zh) 2011-12-26 2014-04-01 Ind Tech Res Inst 複合原料、碳纖維材料及其形成方法
EP4070102A1 (de) * 2019-12-05 2022-10-12 Waters Technologies Corporation Polyphosphonsäuren zur verbesserung der rückgewinnung und minimierung des systemverlustes

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
IT1232311B (it) 1989-09-04 1992-01-28 Sclavo Spa Metodo di purificazione di composti a struttura peptidica o pseudo peptidica a basso peso molecolare.
US5866429A (en) * 1991-04-03 1999-02-02 Bloch; Will Precision and accuracy of anion-exchange separation of nucleic acids
CA2106612C (en) 1993-09-21 2001-02-06 Diana Pliura Displacement chromatography process
US5478924A (en) 1994-02-16 1995-12-26 Cramer; Steven M. Displacement chromatography of proteins using low molecular weight displacers
US5427686A (en) 1994-04-05 1995-06-27 Sri International Process for separating material from mixture using displacement chromatography
US6329145B1 (en) 1999-02-09 2001-12-11 Gilead Science, Inc. Determining non-nucleic acid molecule binding to target by competition with nucleic acid ligand
US6573373B1 (en) * 1999-06-29 2003-06-03 Isis Pharmaceuticals, Inc. High affinity, low molecular weight displacers for oligonucleotide purification

Also Published As

Publication number Publication date
WO2001000642A9 (en) 2002-07-25
JP2003503418A (ja) 2003-01-28
US6828436B2 (en) 2004-12-07
AU5774000A (en) 2001-01-31
US20020156267A1 (en) 2002-10-24
WO2001000642A1 (en) 2001-01-04
EP1192172B1 (de) 2007-05-02
US6573373B1 (en) 2003-06-03
EP1192172A1 (de) 2002-04-03
DE60034676T2 (de) 2008-01-17
DE60034676D1 (de) 2007-06-14
EP1192172A4 (de) 2002-08-28

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