ATE362532T1 - Verfahren zur amplifizierung einer nukleinsäuresequenz unter verwendung eines chimären primers - Google Patents

Verfahren zur amplifizierung einer nukleinsäuresequenz unter verwendung eines chimären primers

Info

Publication number
ATE362532T1
ATE362532T1 AT00908056T AT00908056T ATE362532T1 AT E362532 T1 ATE362532 T1 AT E362532T1 AT 00908056 T AT00908056 T AT 00908056T AT 00908056 T AT00908056 T AT 00908056T AT E362532 T1 ATE362532 T1 AT E362532T1
Authority
AT
Austria
Prior art keywords
nucleic acid
acid sequence
amplifying
detecting
chimeric primer
Prior art date
Application number
AT00908056T
Other languages
English (en)
Inventor
Hiroyuki Mukai
Junko Yamamoto
Osamu Takeda
Kazue Miyake
Takashi Uemori
Yoshimi Sato
Mariko Moriyama
Haruhisa Sawaragi
Michio Hagiya
Kiyozo Asada
Ikunoshin Kato
Original Assignee
Takara Bio Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Takara Bio Inc filed Critical Takara Bio Inc
Application granted granted Critical
Publication of ATE362532T1 publication Critical patent/ATE362532T1/de

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • General Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
AT00908056T 1999-03-19 2000-03-14 Verfahren zur amplifizierung einer nukleinsäuresequenz unter verwendung eines chimären primers ATE362532T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP7696699 1999-03-19
JP37003599 1999-12-27

Publications (1)

Publication Number Publication Date
ATE362532T1 true ATE362532T1 (de) 2007-06-15

Family

ID=26418069

Family Applications (1)

Application Number Title Priority Date Filing Date
AT00908056T ATE362532T1 (de) 1999-03-19 2000-03-14 Verfahren zur amplifizierung einer nukleinsäuresequenz unter verwendung eines chimären primers

Country Status (11)

Country Link
EP (1) EP1167524B1 (de)
JP (1) JP3433929B2 (de)
KR (1) KR100682576B1 (de)
CN (1) CN1227357C (de)
AT (1) ATE362532T1 (de)
AU (1) AU773536B2 (de)
CA (1) CA2365135C (de)
DE (1) DE60034878T2 (de)
EA (2) EA004327B1 (de)
TW (1) TWI259204B (de)
WO (1) WO2000056877A1 (de)

Families Citing this family (69)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7846733B2 (en) 2000-06-26 2010-12-07 Nugen Technologies, Inc. Methods and compositions for transcription-based nucleic acid amplification
AU7878301A (en) * 2000-08-23 2002-03-04 Takara Shuzo Co Method of amplifying nucleic acid
KR100689795B1 (ko) * 2000-09-19 2007-03-09 다카라 바이오 가부시키가이샤 복합체 형성 방법
EP1427847B1 (de) 2000-12-13 2010-07-28 Nugen Technologies, Inc. Methoden und zusammensetzungen zur generierung einer vielzahl von kopien von nukleinsäuresequenzen und methoden zur detektion derselben
EP1347060A4 (de) * 2000-12-26 2004-08-18 Takara Bio Inc Verfahren zum nachweis pathogener mikroorganismen
EP1359219A4 (de) * 2001-02-06 2004-09-22 Takara Bio Inc Amplifizierte nukleinsäuren und immobilisierte produkte davon
KR100809949B1 (ko) * 2001-02-15 2008-03-06 다카라 바이오 가부시키가이샤 염기 치환의 검출 방법
JP2002253257A (ja) * 2001-03-02 2002-09-10 Tosoh Corp ベロ毒素検出のためのオリゴヌクレオチドおよび検出法
MXPA02012739A (es) 2001-03-09 2004-04-20 Nugen Technologies Inc Metodos y composiciones para amplificacion de secuencias de arn.
EP1390537B1 (de) 2001-03-09 2013-11-13 Nugen Technologies, Inc. Verfahren und zusammensetzungen zur amplifikation von rna-sequenzen
AU2003303395A1 (en) 2001-05-22 2004-07-22 Dahl, Gary, A. Target-dependent transcription using deletion mutants of n4 rna polymerase
US8137911B2 (en) 2001-05-22 2012-03-20 Cellscript, Inc. Preparation and use of single-stranded transcription substrates for synthesis of transcription products corresponding to target sequences
EA006679B1 (ru) * 2001-06-12 2006-02-24 Такара Био Инк. Способ стабилизации реагента для амплификации или детекции нуклеиновой кислоты и способ хранения
WO2002101041A1 (en) * 2001-06-12 2002-12-19 Takara Bio Inc. Method of amplifying nucleic acid and method of detecting nucleotide polymorphism with the use of nucleotide analog
US7056671B2 (en) 2001-08-20 2006-06-06 Takara Bio Inc. Isothermal chimeric primer nucleic acid amplification methods using blocking oglionucleotide
JP2005102502A (ja) * 2001-11-21 2005-04-21 Wakunaga Pharmaceut Co Ltd 一本鎖目的核酸断片の増幅方法
WO2003074696A1 (en) * 2002-03-07 2003-09-12 Takara Bio Inc. Method of detecting base substitution
JP4105693B2 (ja) 2002-08-30 2008-06-25 タカラバイオ株式会社 耐熱性リボヌクレアーゼｈ
AU2003297557B2 (en) 2002-11-21 2009-02-26 Cellscript, Inc. Methods for using primers that encode one strand of a double-stranded promoter
JP2006523465A (ja) 2003-04-14 2006-10-19 ニューゲン テクノロジーズ， インコーポレイテッド ランダムにプライミングされる複合プライマーを用いる大規模増幅
US20050239087A1 (en) * 2003-04-29 2005-10-27 Haiguang Xiao Multiply-primed amplification of nucleic acid sequences
CN100434502C (zh) * 2003-07-11 2008-11-19 太阳诱电株式会社 核酸扩增装置及核酸扩增方法
CN1890368A (zh) 2003-12-10 2007-01-03 宝生物工程株式会社 扩增核酸的方法
JP4548174B2 (ja) 2005-03-24 2010-09-22 コニカミノルタエムジー株式会社 検査用マイクロチップおよびそれを用いた検査装置
EP1887363A4 (de) 2005-04-01 2012-08-22 Konica Minolta Med & Graphic Mikrogesamtanalysesystem, inspektionschip und inspektionsverfahren
EP1883707B1 (de) 2005-05-26 2014-04-09 Human Genetic Signatures PTY Ltd Isothermische strangverdrängungsamplifikation unter verwendung von eine nichtreguläre base enthaltenden primern
EP1929046B1 (de) 2005-09-07 2012-07-11 Nugen Technologies, Inc. Verbessertes nukleinsäureamplifikationsverfahren
US8133456B2 (en) 2005-11-07 2012-03-13 Konica Minolta Medical & Graphic, Inc. Microreactor and method of liquid feeding making use of the same
JP2009219355A (ja) * 2006-07-05 2009-10-01 Synthera Technologies Co Ltd 標的物質の検出方法
JP4918409B2 (ja) 2006-07-26 2012-04-18 西川ゴム工業株式会社 核酸配列の増幅方法
US8202972B2 (en) 2007-01-10 2012-06-19 General Electric Company Isothermal DNA amplification
JP4340298B2 (ja) 2007-03-01 2009-10-07 株式会社日立ハイテクノロジーズ 核酸回収方法及び核酸回収装置
US8143006B2 (en) 2007-08-03 2012-03-27 Igor Kutyavin Accelerated cascade amplification (ACA) of nucleic acids comprising strand and sequence specific DNA nicking
EP2202305A4 (de) 2007-10-04 2011-01-19 Tosoh Corp Primer zur rrna-amplifikation eines bakteriums der gattung legionella, nachweisverfahren und nachweis-kit
US8034568B2 (en) 2008-02-12 2011-10-11 Nugen Technologies, Inc. Isothermal nucleic acid amplification methods and compositions
US7846666B2 (en) 2008-03-21 2010-12-07 Nugen Technologies, Inc. Methods of RNA amplification in the presence of DNA
JP5596684B2 (ja) * 2008-08-12 2014-09-24 エフ．ホフマン−ラ ロシュ アーゲー プルーフリーディング・プライマー伸長
US9080211B2 (en) 2008-10-24 2015-07-14 Epicentre Technologies Corporation Transposon end compositions and methods for modifying nucleic acids
EP2664678B1 (de) 2008-10-24 2014-10-08 Epicentre Technologies Corporation Transposonendenzusammensetzungen und Verfahren zur Modifizierung von Nukleinsäuren
US20110236900A1 (en) 2008-11-27 2011-09-29 Riken Novel muts protein and method for determing mutation using the same
WO2011133695A2 (en) * 2010-04-20 2011-10-27 Swift Biosciences, Inc. Materials and methods for nucleic acid fractionation by solid phase entrapment and enzyme-mediated detachment
JP5454338B2 (ja) 2010-04-28 2014-03-26 株式会社島津製作所 液滴操作によるリアルタイム核酸増幅法
US9517472B2 (en) 2010-12-21 2016-12-13 Shimadzu Corporation Device and method for processing target component in tube
JP5807542B2 (ja) 2011-12-22 2015-11-10 株式会社島津製作所 対象成分を操作するためのチップデバイス及びそれを用いた方法
US9777319B2 (en) 2012-06-29 2017-10-03 General Electric Company Method for isothermal DNA amplification starting from an RNA template
WO2014051076A1 (ja) 2012-09-28 2014-04-03 株式会社Ｂｎａ Ｂｎａクランプ法
US20150275285A1 (en) * 2012-12-03 2015-10-01 Yilin Zhang Compositions and methods of nucleic acid preparation and analyses
CN105026576A (zh) * 2012-12-03 2015-11-04 以琳生物药物有限公司 单链多核苷酸扩增方法
KR101589483B1 (ko) * 2014-02-21 2016-01-28 디엑스진주식회사 핵산과 신호 프로브의 비대칭 등온증폭을 이용한 핵산의 검출방법
US10017759B2 (en) 2014-06-26 2018-07-10 Illumina, Inc. Library preparation of tagged nucleic acid
SG11201703779VA (en) 2014-11-11 2017-06-29 Illumina Inc Polynucleotide amplification using crispr-cas systems
US11046939B2 (en) 2015-11-27 2021-06-29 Kyushu University, National University Corporation DNA polymerase variant
EP3656873A3 (de) 2016-05-11 2020-07-29 Illumina, Inc. Polynukleotidanreicherung und -amplifikation unter verwendung von argonautensystemen
GB201612011D0 (en) 2016-07-11 2016-08-24 Glaxosmithkline Ip Dev Ltd Novel processes for the production of oligonucleotides
WO2018129368A2 (en) 2017-01-06 2018-07-12 Editas Medicine, Inc. Methods of assessing nuclease cleavage
US10914729B2 (en) 2017-05-22 2021-02-09 The Trustees Of Princeton University Methods for detecting protein binding sequences and tagging nucleic acids
GB201721307D0 (en) 2017-12-19 2018-01-31 Glaxosmithkline Ip Dev Ltd Novel processes for the production of oligonucleotides
CN108642144B (zh) * 2018-05-18 2020-06-09 贠红岩 一种恒温链置换扩增技术及试剂盒
EP3812461A4 (de) 2018-06-21 2022-03-23 Public University Corporation Nagoya City University Magenkrebs-biomarker und verwendung davon
CN108913736B (zh) * 2018-07-10 2021-08-24 中国海洋大学 单链寡核苷酸的制备方法
US20220325354A1 (en) 2019-07-29 2022-10-13 Public University Corporation Nagoya City University Esophageal cancer biomarker and use therefor
TWI732404B (zh) * 2019-12-30 2021-07-01 源點生物科技股份有限公司 使用酶製備核酸序列的裝置及方法
TWI732405B (zh) * 2019-12-30 2021-07-01 源點生物科技股份有限公司 使用酶製備核酸序列的方法
KR20230154078A (ko) 2021-03-09 2023-11-07 일루미나, 인코포레이티드 CAS-gRNA 리보핵단백질을 사용한 게놈 라이브러리 제작 및 표적화된 후성적 검정
KR102809622B1 (ko) 2021-03-09 2025-05-19 일루미나, 인코포레이티드 세포에서 단백질-코딩 변이체의 발현 분석
EP4614146A3 (de) 2021-08-11 2025-11-12 Illumina, Inc. Nachweis von analyten unter verwendung gezielter epigenetischer tests, proximitätsinduzierter tagmentation, stranginvasion, -einschränkung oder ligation
DE102022211087A1 (de) * 2022-10-19 2024-04-25 Robert Bosch Gesellschaft mit beschränkter Haftung Verfahren und Vorrichtung zur Voramplifikation eines Nukleinsäuregemisches
JP2025023671A (ja) 2023-08-04 2025-02-17 シスメックス株式会社 Ｂｓｔ ＤＮＡポリメラーゼ、試薬組成物及び核酸増幅方法
CN118207295A (zh) * 2024-04-10 2024-06-18 浙江省人民医院 一种快速特异性逆转录试剂及其使用方法

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5648211A (en) * 1994-04-18 1997-07-15 Becton, Dickinson And Company Strand displacement amplification using thermophilic enzymes
KR19990022596A (ko) * 1995-06-07 1999-03-25 다니엘 엘. 캐시앙, 헨리 엘. 노르호프 효소적으로 절단 가능한 주형 및 시발체 기제 올리고뉴클레오티드의 합성
FR2737223B1 (fr) * 1995-07-24 1997-09-12 Bio Merieux Procede d'amplification de sequences d'acide nucleique par deplacement, a l'aide d'amorces chimeres
GB9717061D0 (en) * 1997-08-13 1997-10-15 Tepnel Medical Ltd Amplification of nucleic acids
DE19813624B4 (de) * 1998-03-27 2004-11-11 Motorenfabrik Hatz Gmbh & Co Kg Luftgekühlter Verbrennungsmotor mit um eine vertikale Achse rotierender Kurbelwelle, insbesondere Einzylinder-Dieselmotor

Also Published As

Publication number Publication date
AU773536B2 (en) 2004-05-27
EP1167524A1 (de) 2002-01-02
AU2944200A (en) 2000-10-09
CN1350581A (zh) 2002-05-22
TWI259204B (en) 2006-08-01
EA005577B1 (ru) 2005-04-28
CA2365135A1 (en) 2000-09-28
WO2000056877A1 (en) 2000-09-28
DE60034878T2 (de) 2008-01-17
EA200300991A1 (ru) 2004-02-26
DE60034878D1 (de) 2007-06-28
CN1227357C (zh) 2005-11-16
JP3433929B2 (ja) 2003-08-04
EA200100990A1 (ru) 2002-02-28
KR20010104720A (ko) 2001-11-26
EP1167524B1 (de) 2007-05-16
EP1167524A4 (de) 2005-03-09
CA2365135C (en) 2009-08-18
KR100682576B1 (ko) 2007-02-15
EA004327B1 (ru) 2004-04-29

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