ATE362532T1 - Verfahren zur amplifizierung einer nukleinsäuresequenz unter verwendung eines chimären primers - Google Patents
Verfahren zur amplifizierung einer nukleinsäuresequenz unter verwendung eines chimären primersInfo
- Publication number
- ATE362532T1 ATE362532T1 AT00908056T AT00908056T ATE362532T1 AT E362532 T1 ATE362532 T1 AT E362532T1 AT 00908056 T AT00908056 T AT 00908056T AT 00908056 T AT00908056 T AT 00908056T AT E362532 T1 ATE362532 T1 AT E362532T1
- Authority
- AT
- Austria
- Prior art keywords
- nucleic acid
- acid sequence
- amplifying
- detecting
- chimeric primer
- Prior art date
Links
- 238000000034 method Methods 0.000 title abstract 9
- 108091028043 Nucleic acid sequence Proteins 0.000 title abstract 5
- 150000007523 nucleic acids Chemical group 0.000 title abstract 5
- 230000004544 DNA amplification Effects 0.000 abstract 2
- 239000012634 fragment Substances 0.000 abstract 2
- 241000894006 Bacteria Species 0.000 abstract 1
- 239000003155 DNA primer Substances 0.000 abstract 1
- 230000006820 DNA synthesis Effects 0.000 abstract 1
- 241000233866 Fungi Species 0.000 abstract 1
- 240000004808 Saccharomyces cerevisiae Species 0.000 abstract 1
- 241000700605 Viruses Species 0.000 abstract 1
- 230000003321 amplification Effects 0.000 abstract 1
- 238000011065 in-situ storage Methods 0.000 abstract 1
- 244000005700 microbiome Species 0.000 abstract 1
- 238000003199 nucleic acid amplification method Methods 0.000 abstract 1
- 238000003786 synthesis reaction Methods 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Biophysics (AREA)
- General Engineering & Computer Science (AREA)
- Immunology (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Analytical Chemistry (AREA)
- Physics & Mathematics (AREA)
- Genetics & Genomics (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- General Health & Medical Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP7696699 | 1999-03-19 | ||
| JP37003599 | 1999-12-27 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ATE362532T1 true ATE362532T1 (de) | 2007-06-15 |
Family
ID=26418069
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AT00908056T ATE362532T1 (de) | 1999-03-19 | 2000-03-14 | Verfahren zur amplifizierung einer nukleinsäuresequenz unter verwendung eines chimären primers |
Country Status (11)
| Country | Link |
|---|---|
| EP (1) | EP1167524B1 (de) |
| JP (1) | JP3433929B2 (de) |
| KR (1) | KR100682576B1 (de) |
| CN (1) | CN1227357C (de) |
| AT (1) | ATE362532T1 (de) |
| AU (1) | AU773536B2 (de) |
| CA (1) | CA2365135C (de) |
| DE (1) | DE60034878T2 (de) |
| EA (2) | EA005577B1 (de) |
| TW (1) | TWI259204B (de) |
| WO (1) | WO2000056877A1 (de) |
Families Citing this family (68)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US7846733B2 (en) | 2000-06-26 | 2010-12-07 | Nugen Technologies, Inc. | Methods and compositions for transcription-based nucleic acid amplification |
| KR100735131B1 (ko) * | 2000-08-23 | 2007-07-03 | 다카라 바이오 가부시키가이샤 | 핵산 증폭 방법 |
| EA004630B1 (ru) * | 2000-09-19 | 2004-06-24 | Такара Био Инк. | Способ образования комплекса |
| AU2002228974A1 (en) | 2000-12-13 | 2002-06-24 | Nugen Technologies, Inc | Methods and compositions for generation of multiple copies of nucleic acid sequences and methods of detection thereof |
| EP1347060A4 (de) * | 2000-12-26 | 2004-08-18 | Takara Bio Inc | Verfahren zum nachweis pathogener mikroorganismen |
| EP1359219A4 (de) * | 2001-02-06 | 2004-09-22 | Takara Bio Inc | Amplifizierte nukleinsäuren und immobilisierte produkte davon |
| CN100351393C (zh) | 2001-02-15 | 2007-11-28 | 宝生物工程株式会社 | 核苷酸多态性的检测方法 |
| JP2002253257A (ja) * | 2001-03-02 | 2002-09-10 | Tosoh Corp | ベロ毒素検出のためのオリゴヌクレオチドおよび検出法 |
| US6946251B2 (en) | 2001-03-09 | 2005-09-20 | Nugen Technologies, Inc. | Methods and compositions for amplification of RNA sequences using RNA-DNA composite primers |
| CA2439074A1 (en) | 2001-03-09 | 2002-09-19 | Nugen Technologies, Inc. | Methods and compositions for amplification of rna sequences |
| WO2004059289A2 (en) | 2001-05-22 | 2004-07-15 | Epicentre Technologies | Target-dependent transcription using deletion mutants of n4 rna polymerase |
| US8137911B2 (en) | 2001-05-22 | 2012-03-20 | Cellscript, Inc. | Preparation and use of single-stranded transcription substrates for synthesis of transcription products corresponding to target sequences |
| CN100379861C (zh) * | 2001-06-12 | 2008-04-09 | 宝生物工程株式会社 | 核酸扩增或检测用试剂的稳定化方法和保存方法 |
| WO2002101041A1 (en) * | 2001-06-12 | 2002-12-19 | Takara Bio Inc. | Method of amplifying nucleic acid and method of detecting nucleotide polymorphism with the use of nucleotide analog |
| US7056671B2 (en) | 2001-08-20 | 2006-06-06 | Takara Bio Inc. | Isothermal chimeric primer nucleic acid amplification methods using blocking oglionucleotide |
| JP2005102502A (ja) * | 2001-11-21 | 2005-04-21 | Wakunaga Pharmaceut Co Ltd | 一本鎖目的核酸断片の増幅方法 |
| WO2003074696A1 (en) * | 2002-03-07 | 2003-09-12 | Takara Bio Inc. | Method of detecting base substitution |
| AU2003257699A1 (en) | 2002-08-30 | 2004-03-19 | Takara Bio Inc. | Thermostable ribonuclease h |
| EP1576188B1 (de) * | 2002-11-21 | 2008-10-15 | Epicentre Technologies | Verfahren zur verwendung von riboprimern zur strangverdrängungsreplikation von zielsequenzen |
| WO2004092418A2 (en) | 2003-04-14 | 2004-10-28 | Nugen Technologies, Inc. | Global amplification using a randomly primed composite primer |
| CA2521520A1 (en) * | 2003-04-29 | 2004-11-11 | Amersham Biosciences Corp | Multiply-primed amplification of nucleic acid sequences |
| US20060257878A1 (en) * | 2003-07-11 | 2006-11-16 | Taiyo Yuden Co., Ltd. | Nucleic acid amplifier and method of nucleic acid amplification |
| ATE458044T1 (de) | 2003-12-10 | 2010-03-15 | Takara Bio Inc | Verfahren zur nukleinsäureamplifikation |
| JP4548174B2 (ja) | 2005-03-24 | 2010-09-22 | コニカミノルタエムジー株式会社 | 検査用マイクロチップおよびそれを用いた検査装置 |
| EP2597472A3 (de) | 2005-04-01 | 2014-03-05 | Konica Minolta Medical & Graphic, Inc. | Mikrointegriertes Analysesystem, Prüfchip und Prüfverfahren |
| AU2006251866B2 (en) | 2005-05-26 | 2007-11-29 | Human Genetic Signatures Pty Ltd | Isothermal strand displacement amplification using primers containing a non-regular base |
| EP1929046B1 (de) | 2005-09-07 | 2012-07-11 | Nugen Technologies, Inc. | Verbessertes nukleinsäureamplifikationsverfahren |
| WO2007052471A1 (ja) | 2005-11-07 | 2007-05-10 | Konica Minolta Medical & Graphic, Inc. | マイクロリアクタおよびそれを用いた送液方法 |
| JP2009219355A (ja) * | 2006-07-05 | 2009-10-01 | Synthera Technologies Co Ltd | 標的物質の検出方法 |
| JP4918409B2 (ja) | 2006-07-26 | 2012-04-18 | 西川ゴム工業株式会社 | 核酸配列の増幅方法 |
| US8202972B2 (en) | 2007-01-10 | 2012-06-19 | General Electric Company | Isothermal DNA amplification |
| JP4340298B2 (ja) | 2007-03-01 | 2009-10-07 | 株式会社日立ハイテクノロジーズ | 核酸回収方法及び核酸回収装置 |
| US8143006B2 (en) | 2007-08-03 | 2012-03-27 | Igor Kutyavin | Accelerated cascade amplification (ACA) of nucleic acids comprising strand and sequence specific DNA nicking |
| JPWO2009044773A1 (ja) | 2007-10-04 | 2011-02-10 | 東ソー株式会社 | レジオネラ属菌rRNA増幅用プライマー、検出方法および検出キット |
| US20090203531A1 (en) | 2008-02-12 | 2009-08-13 | Nurith Kurn | Method for Archiving and Clonal Expansion |
| WO2009117698A2 (en) | 2008-03-21 | 2009-09-24 | Nugen Technologies, Inc. | Methods of rna amplification in the presence of dna |
| ES2384681T3 (es) | 2008-08-12 | 2012-07-10 | F. Hoffmann-La Roche Ag | Extensión de cebadores correctora |
| US9080211B2 (en) | 2008-10-24 | 2015-07-14 | Epicentre Technologies Corporation | Transposon end compositions and methods for modifying nucleic acids |
| DK2787565T3 (en) | 2008-10-24 | 2015-10-12 | Epict Technologies Corp | Transposon-end COMPOSITIONS AND METHODS TO MODIFY nucleic acids |
| JPWO2010061922A1 (ja) | 2008-11-27 | 2012-04-26 | 独立行政法人理化学研究所 | 新規MutSタンパク質およびそれを用いた変異の判定方法 |
| US20110269194A1 (en) * | 2010-04-20 | 2011-11-03 | Swift Biosciences, Inc. | Materials and methods for nucleic acid fractionation by solid phase entrapment and enzyme-mediated detachment |
| JP5454338B2 (ja) | 2010-04-28 | 2014-03-26 | 株式会社島津製作所 | 液滴操作によるリアルタイム核酸増幅法 |
| US9517472B2 (en) | 2010-12-21 | 2016-12-13 | Shimadzu Corporation | Device and method for processing target component in tube |
| JP5807542B2 (ja) | 2011-12-22 | 2015-11-10 | 株式会社島津製作所 | 対象成分を操作するためのチップデバイス及びそれを用いた方法 |
| US9777319B2 (en) | 2012-06-29 | 2017-10-03 | General Electric Company | Method for isothermal DNA amplification starting from an RNA template |
| CA2886334C (en) | 2012-09-28 | 2021-12-21 | Bna Inc. | Bna clamp method |
| WO2014088979A1 (en) * | 2012-12-03 | 2014-06-12 | Yilin Zhang | Compositions and methods of nucleic acid preparation and analyses |
| CN105026576A (zh) * | 2012-12-03 | 2015-11-04 | 以琳生物药物有限公司 | 单链多核苷酸扩增方法 |
| KR101589483B1 (ko) * | 2014-02-21 | 2016-01-28 | 디엑스진주식회사 | 핵산과 신호 프로브의 비대칭 등온증폭을 이용한 핵산의 검출방법 |
| US10017759B2 (en) | 2014-06-26 | 2018-07-10 | Illumina, Inc. | Library preparation of tagged nucleic acid |
| WO2016077350A1 (en) | 2014-11-11 | 2016-05-19 | Illumina, Inc. | Polynucleotide amplification using crispr-cas systems |
| CN108473970B (zh) | 2015-11-27 | 2022-09-13 | 国立大学法人九州大学 | Dna聚合酶变体 |
| WO2017197027A1 (en) | 2016-05-11 | 2017-11-16 | Illumina, Inc. | Polynucleotide enrichment and amplification using argonaute systems |
| GB201612011D0 (en) | 2016-07-11 | 2016-08-24 | Glaxosmithkline Ip Dev Ltd | Novel processes for the production of oligonucleotides |
| WO2018129368A2 (en) | 2017-01-06 | 2018-07-12 | Editas Medicine, Inc. | Methods of assessing nuclease cleavage |
| US10914729B2 (en) | 2017-05-22 | 2021-02-09 | The Trustees Of Princeton University | Methods for detecting protein binding sequences and tagging nucleic acids |
| CN108642144B (zh) * | 2018-05-18 | 2020-06-09 | 贠红岩 | 一种恒温链置换扩增技术及试剂盒 |
| EP3812461A4 (de) | 2018-06-21 | 2022-03-23 | Public University Corporation Nagoya City University | Magenkrebs-biomarker und verwendung davon |
| CN108913736B (zh) * | 2018-07-10 | 2021-08-24 | 中国海洋大学 | 单链寡核苷酸的制备方法 |
| WO2021019944A1 (ja) | 2019-07-29 | 2021-02-04 | 公立大学法人名古屋市立大学 | 食道癌バイオマーカー及びその用途 |
| TWI732404B (zh) * | 2019-12-30 | 2021-07-01 | 源點生物科技股份有限公司 | 使用酶製備核酸序列的裝置及方法 |
| TWI732405B (zh) * | 2019-12-30 | 2021-07-01 | 源點生物科技股份有限公司 | 使用酶製備核酸序列的方法 |
| WO2022192191A1 (en) | 2021-03-09 | 2022-09-15 | Illumina, Inc. | Analyzing expression of protein-coding variants in cells |
| IL305444B2 (en) | 2021-03-09 | 2025-02-01 | Illumina Inc | Genomic library preparation and targeted epigenetic testing using Cas–Grna ribonucleoproteins |
| CA3223731A1 (en) | 2021-08-11 | 2023-02-16 | Illumina, Inc. | Detection of analytes using targeted epigenetic assays, proximity-induced tagmentation, strand invasion, restriction, or ligation |
| DE102022211087A1 (de) * | 2022-10-19 | 2024-04-25 | Robert Bosch Gesellschaft mit beschränkter Haftung | Verfahren und Vorrichtung zur Voramplifikation eines Nukleinsäuregemisches |
| JP2025023671A (ja) | 2023-08-04 | 2025-02-17 | シスメックス株式会社 | Bst DNAポリメラーゼ、試薬組成物及び核酸増幅方法 |
| CN118207295A (zh) * | 2024-04-10 | 2024-06-18 | 浙江省人民医院 | 一种快速特异性逆转录试剂及其使用方法 |
Family Cites Families (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5648211A (en) * | 1994-04-18 | 1997-07-15 | Becton, Dickinson And Company | Strand displacement amplification using thermophilic enzymes |
| WO1996040901A1 (en) * | 1995-06-07 | 1996-12-19 | Gen-Probe Incorporated | Template and primer based synthesis of enzymatically cleavable oligonucleotides |
| FR2737223B1 (fr) * | 1995-07-24 | 1997-09-12 | Bio Merieux | Procede d'amplification de sequences d'acide nucleique par deplacement, a l'aide d'amorces chimeres |
| GB9717061D0 (en) * | 1997-08-13 | 1997-10-15 | Tepnel Medical Ltd | Amplification of nucleic acids |
| DE19813624B4 (de) * | 1998-03-27 | 2004-11-11 | Motorenfabrik Hatz Gmbh & Co Kg | Luftgekühlter Verbrennungsmotor mit um eine vertikale Achse rotierender Kurbelwelle, insbesondere Einzylinder-Dieselmotor |
-
2000
- 2000-03-14 CN CNB008075344A patent/CN1227357C/zh not_active Expired - Fee Related
- 2000-03-14 EP EP00908056A patent/EP1167524B1/de not_active Expired - Lifetime
- 2000-03-14 AU AU29442/00A patent/AU773536B2/en not_active Ceased
- 2000-03-14 KR KR1020017011331A patent/KR100682576B1/ko not_active Expired - Fee Related
- 2000-03-14 AT AT00908056T patent/ATE362532T1/de not_active IP Right Cessation
- 2000-03-14 JP JP2000606736A patent/JP3433929B2/ja not_active Expired - Fee Related
- 2000-03-14 EA EA200300991A patent/EA005577B1/ru not_active IP Right Cessation
- 2000-03-14 DE DE60034878T patent/DE60034878T2/de not_active Expired - Lifetime
- 2000-03-14 WO PCT/JP2000/001534 patent/WO2000056877A1/ja not_active Ceased
- 2000-03-14 CA CA002365135A patent/CA2365135C/en not_active Expired - Fee Related
- 2000-03-14 EA EA200100990A patent/EA004327B1/ru not_active IP Right Cessation
- 2000-03-17 TW TW089104975A patent/TWI259204B/zh not_active IP Right Cessation
Also Published As
| Publication number | Publication date |
|---|---|
| CN1350581A (zh) | 2002-05-22 |
| AU2944200A (en) | 2000-10-09 |
| KR100682576B1 (ko) | 2007-02-15 |
| KR20010104720A (ko) | 2001-11-26 |
| EA004327B1 (ru) | 2004-04-29 |
| CA2365135A1 (en) | 2000-09-28 |
| EP1167524A1 (de) | 2002-01-02 |
| EA200300991A1 (ru) | 2004-02-26 |
| DE60034878D1 (de) | 2007-06-28 |
| EP1167524B1 (de) | 2007-05-16 |
| WO2000056877A1 (en) | 2000-09-28 |
| AU773536B2 (en) | 2004-05-27 |
| EP1167524A4 (de) | 2005-03-09 |
| DE60034878T2 (de) | 2008-01-17 |
| CA2365135C (en) | 2009-08-18 |
| EA005577B1 (ru) | 2005-04-28 |
| EA200100990A1 (ru) | 2002-02-28 |
| CN1227357C (zh) | 2005-11-16 |
| TWI259204B (en) | 2006-08-01 |
| JP3433929B2 (ja) | 2003-08-04 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| RER | Ceased as to paragraph 5 lit. 3 law introducing patent treaties |