ATE362532T1 - Verfahren zur amplifizierung einer nukleinsäuresequenz unter verwendung eines chimären primers - Google Patents

Verfahren zur amplifizierung einer nukleinsäuresequenz unter verwendung eines chimären primers

Info

Publication number
ATE362532T1
ATE362532T1 AT00908056T AT00908056T ATE362532T1 AT E362532 T1 ATE362532 T1 AT E362532T1 AT 00908056 T AT00908056 T AT 00908056T AT 00908056 T AT00908056 T AT 00908056T AT E362532 T1 ATE362532 T1 AT E362532T1
Authority
AT
Austria
Prior art keywords
nucleic acid
acid sequence
amplifying
detecting
chimeric primer
Prior art date
Application number
AT00908056T
Other languages
English (en)
Inventor
Hiroyuki Mukai
Junko Yamamoto
Osamu Takeda
Kazue Miyake
Takashi Uemori
Yoshimi Sato
Mariko Moriyama
Haruhisa Sawaragi
Michio Hagiya
Kiyozo Asada
Ikunoshin Kato
Original Assignee
Takara Bio Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Takara Bio Inc filed Critical Takara Bio Inc
Application granted granted Critical
Publication of ATE362532T1 publication Critical patent/ATE362532T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • General Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
AT00908056T 1999-03-19 2000-03-14 Verfahren zur amplifizierung einer nukleinsäuresequenz unter verwendung eines chimären primers ATE362532T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP7696699 1999-03-19
JP37003599 1999-12-27

Publications (1)

Publication Number Publication Date
ATE362532T1 true ATE362532T1 (de) 2007-06-15

Family

ID=26418069

Family Applications (1)

Application Number Title Priority Date Filing Date
AT00908056T ATE362532T1 (de) 1999-03-19 2000-03-14 Verfahren zur amplifizierung einer nukleinsäuresequenz unter verwendung eines chimären primers

Country Status (11)

Country Link
EP (1) EP1167524B1 (de)
JP (1) JP3433929B2 (de)
KR (1) KR100682576B1 (de)
CN (1) CN1227357C (de)
AT (1) ATE362532T1 (de)
AU (1) AU773536B2 (de)
CA (1) CA2365135C (de)
DE (1) DE60034878T2 (de)
EA (2) EA005577B1 (de)
TW (1) TWI259204B (de)
WO (1) WO2000056877A1 (de)

Families Citing this family (68)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7846733B2 (en) 2000-06-26 2010-12-07 Nugen Technologies, Inc. Methods and compositions for transcription-based nucleic acid amplification
AU2001278783B2 (en) * 2000-08-23 2006-04-27 Takara Bio Inc. Method of amplifying nucleic acid
CA2422736A1 (en) * 2000-09-19 2003-03-18 Takara Bio Inc. Method of forming complex
WO2002048402A2 (en) 2000-12-13 2002-06-20 Nugen Technologies, Inc. Methods and compositions for generation of multiple copies of nucleic acid sequences and methods of detection thereof
EP1347060A4 (de) * 2000-12-26 2004-08-18 Takara Bio Inc Verfahren zum nachweis pathogener mikroorganismen
JPWO2002062993A1 (ja) * 2001-02-06 2004-06-10 タカラバイオ株式会社 増幅核酸及びその固定化物
JP3681729B2 (ja) * 2001-02-15 2005-08-10 タカラバイオ株式会社 塩基置換の検出方法
JP2002253257A (ja) * 2001-03-02 2002-09-10 Tosoh Corp ベロ毒素検出のためのオリゴヌクレオチドおよび検出法
CA2439074A1 (en) 2001-03-09 2002-09-19 Nugen Technologies, Inc. Methods and compositions for amplification of rna sequences
KR20030082535A (ko) 2001-03-09 2003-10-22 뉴젠 테크놀로지스 인코포레이티드 Ｒｎａ 서열의 증폭을 위한 방법 및 조성물
WO2004059289A2 (en) 2001-05-22 2004-07-15 Epicentre Technologies Target-dependent transcription using deletion mutants of n4 rna polymerase
US8137911B2 (en) 2001-05-22 2012-03-20 Cellscript, Inc. Preparation and use of single-stranded transcription substrates for synthesis of transcription products corresponding to target sequences
WO2002101041A1 (en) * 2001-06-12 2002-12-19 Takara Bio Inc. Method of amplifying nucleic acid and method of detecting nucleotide polymorphism with the use of nucleotide analog
EA006679B1 (ru) * 2001-06-12 2006-02-24 Такара Био Инк. Способ стабилизации реагента для амплификации или детекции нуклеиновой кислоты и способ хранения
JPWO2003016569A1 (ja) 2001-08-20 2004-12-02 タカラバイオ株式会社 核酸の増幅方法
JP2005102502A (ja) * 2001-11-21 2005-04-21 Wakunaga Pharmaceut Co Ltd 一本鎖目的核酸断片の増幅方法
WO2003074696A1 (en) * 2002-03-07 2003-09-12 Takara Bio Inc. Method of detecting base substitution
WO2004020621A1 (ja) 2002-08-30 2004-03-11 Takara Bio Inc. 耐熱性リボヌクレアーゼh
US7413857B2 (en) 2002-11-21 2008-08-19 Epicentre Technologies Methods for using riboprimers for strand displacement replication of target sequences
JP2006523465A (ja) 2003-04-14 2006-10-19 ニューゲン テクノロジーズ， インコーポレイテッド ランダムにプライミングされる複合プライマーを用いる大規模増幅
JP2006525023A (ja) * 2003-04-29 2006-11-09 ジーイー・ヘルスケア・バイオサイエンス・コーポレイション 核酸配列の多重開始増幅
EP1659170A1 (de) * 2003-07-11 2006-05-24 Taiyo Yuden Co., Ltd. Nukleinsäureamplifikationsvorrichtung und - verfahren
CN1890368A (zh) 2003-12-10 2007-01-03 宝生物工程株式会社 扩增核酸的方法
JP4548174B2 (ja) 2005-03-24 2010-09-22 コニカミノルタエムジー株式会社 検査用マイクロチップおよびそれを用いた検査装置
EP2597472A3 (de) 2005-04-01 2014-03-05 Konica Minolta Medical & Graphic, Inc. Mikrointegriertes Analysesystem, Prüfchip und Prüfverfahren
EP1883707B1 (de) 2005-05-26 2014-04-09 Human Genetic Signatures PTY Ltd Isothermische strangverdrängungsamplifikation unter verwendung von eine nichtreguläre base enthaltenden primern
US7939258B2 (en) 2005-09-07 2011-05-10 Nugen Technologies, Inc. Nucleic acid amplification procedure using RNA and DNA composite primers
EP1946830B1 (de) 2005-11-07 2019-04-03 Konica Minolta Medical & Graphic, Inc. Mikroreaktor
JP2009219355A (ja) * 2006-07-05 2009-10-01 Synthera Technologies Co Ltd 標的物質の検出方法
JP4918409B2 (ja) 2006-07-26 2012-04-18 西川ゴム工業株式会社 核酸配列の増幅方法
US8202972B2 (en) 2007-01-10 2012-06-19 General Electric Company Isothermal DNA amplification
JP4340298B2 (ja) 2007-03-01 2009-10-07 株式会社日立ハイテクノロジーズ 核酸回収方法及び核酸回収装置
US8143006B2 (en) 2007-08-03 2012-03-27 Igor Kutyavin Accelerated cascade amplification (ACA) of nucleic acids comprising strand and sequence specific DNA nicking
JPWO2009044773A1 (ja) 2007-10-04 2011-02-10 東ソー株式会社 レジオネラ属菌ｒＲＮＡ増幅用プライマー、検出方法および検出キット
US8034568B2 (en) 2008-02-12 2011-10-11 Nugen Technologies, Inc. Isothermal nucleic acid amplification methods and compositions
GB2470672B (en) 2008-03-21 2012-09-12 Nugen Technologies Inc Methods of RNA amplification in the presence of DNA
JP5596684B2 (ja) * 2008-08-12 2014-09-24 エフ．ホフマン−ラ ロシュ アーゲー プルーフリーディング・プライマー伸長
ES2743029T3 (es) 2008-10-24 2020-02-18 Epicentre Tech Corporation Composiciones de extremo del transposón y métodos para modificar ácidos nucleicos
US9080211B2 (en) 2008-10-24 2015-07-14 Epicentre Technologies Corporation Transposon end compositions and methods for modifying nucleic acids
EP2371951A4 (de) 2008-11-27 2012-07-18 Riken NEUARTIGES MutS-PROTEIN UND SEINE VERWENDUNG ZUR BESTIMMUNG VON MUTATIONEN
WO2011133695A2 (en) * 2010-04-20 2011-10-27 Swift Biosciences, Inc. Materials and methods for nucleic acid fractionation by solid phase entrapment and enzyme-mediated detachment
JP5454338B2 (ja) 2010-04-28 2014-03-26 株式会社島津製作所 液滴操作によるリアルタイム核酸増幅法
CN103269787B9 (zh) 2010-12-21 2016-07-20 株式会社岛津制作所 用于在管内操作对象成分的器件和方法
JP5807542B2 (ja) 2011-12-22 2015-11-10 株式会社島津製作所 対象成分を操作するためのチップデバイス及びそれを用いた方法
US9777319B2 (en) 2012-06-29 2017-10-03 General Electric Company Method for isothermal DNA amplification starting from an RNA template
CA2886334C (en) 2012-09-28 2021-12-21 Bna Inc. Bna clamp method
EP2925893A4 (de) * 2012-12-03 2016-09-07 Elim Biopharmaceuticals Inc Zusammensetzungen und verfahren zur nukleinsäureherstellung und -analyse
US20150299772A1 (en) * 2012-12-03 2015-10-22 Elim Biopharmaceuticals, Inc. Single-stranded polynucleotide amplification methods
KR101589483B1 (ko) * 2014-02-21 2016-01-28 디엑스진주식회사 핵산과 신호 프로브의 비대칭 등온증폭을 이용한 핵산의 검출방법
US10017759B2 (en) 2014-06-26 2018-07-10 Illumina, Inc. Library preparation of tagged nucleic acid
CN107109495B (zh) 2014-11-11 2021-12-24 伊鲁米那股份有限公司 使用crispr-cas系统的多核苷酸扩增
US11046939B2 (en) 2015-11-27 2021-06-29 Kyushu University, National University Corporation DNA polymerase variant
EP3455372B1 (de) 2016-05-11 2019-10-30 Illumina, Inc. Polynukleotidanreicherung und -amplifikation unter verwendung von argonautensystemen
GB201612011D0 (en) 2016-07-11 2016-08-24 Glaxosmithkline Ip Dev Ltd Novel processes for the production of oligonucleotides
EP3565907B1 (de) 2017-01-06 2022-05-04 Editas Medicine, Inc. Verfahren zur beurteilung der nukleasespaltung
US10914729B2 (en) 2017-05-22 2021-02-09 The Trustees Of Princeton University Methods for detecting protein binding sequences and tagging nucleic acids
CN108642144B (zh) * 2018-05-18 2020-06-09 贠红岩 一种恒温链置换扩增技术及试剂盒
US20210269884A1 (en) 2018-06-21 2021-09-02 Public University Corporation Nagoya City University Gastric cancer biomarker and use thereof
CN108913736B (zh) * 2018-07-10 2021-08-24 中国海洋大学 单链寡核苷酸的制备方法
EP4006151A4 (de) 2019-07-29 2023-10-25 Public University Corporation Nagoya City University Biomarker für speiseröhrenkrebs und verwendung davon
TWI732405B (zh) * 2019-12-30 2021-07-01 源點生物科技股份有限公司 使用酶製備核酸序列的方法
TWI732404B (zh) * 2019-12-30 2021-07-01 源點生物科技股份有限公司 使用酶製備核酸序列的裝置及方法
KR20230154078A (ko) 2021-03-09 2023-11-07 일루미나, 인코포레이티드 CAS-gRNA 리보핵단백질을 사용한 게놈 라이브러리 제작 및 표적화된 후성적 검정
BR112023018157A2 (pt) 2021-03-09 2023-10-31 Illumina Inc Análise de expressão de variantes codificantes de proteína em células
ES3035187T3 (en) 2021-08-11 2025-08-29 Illumina Inc Detection of analytes using targeted epigenetic assays, proximity-induced tagmentation, strand invasion, restriction, or ligation
DE102022211087A1 (de) * 2022-10-19 2024-04-25 Robert Bosch Gesellschaft mit beschränkter Haftung Verfahren und Vorrichtung zur Voramplifikation eines Nukleinsäuregemisches
JP2025023671A (ja) 2023-08-04 2025-02-17 シスメックス株式会社 Ｂｓｔ ＤＮＡポリメラーゼ、試薬組成物及び核酸増幅方法
CN118207295A (zh) * 2024-04-10 2024-06-18 浙江省人民医院 一种快速特异性逆转录试剂及其使用方法

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5648211A (en) * 1994-04-18 1997-07-15 Becton, Dickinson And Company Strand displacement amplification using thermophilic enzymes
WO1996040901A1 (en) * 1995-06-07 1996-12-19 Gen-Probe Incorporated Template and primer based synthesis of enzymatically cleavable oligonucleotides
FR2737223B1 (fr) * 1995-07-24 1997-09-12 Bio Merieux Procede d'amplification de sequences d'acide nucleique par deplacement, a l'aide d'amorces chimeres
GB9717061D0 (en) * 1997-08-13 1997-10-15 Tepnel Medical Ltd Amplification of nucleic acids
DE19813624B4 (de) * 1998-03-27 2004-11-11 Motorenfabrik Hatz Gmbh & Co Kg Luftgekühlter Verbrennungsmotor mit um eine vertikale Achse rotierender Kurbelwelle, insbesondere Einzylinder-Dieselmotor

Also Published As

Publication number Publication date
EA005577B1 (ru) 2005-04-28
EA200100990A1 (ru) 2002-02-28
EA200300991A1 (ru) 2004-02-26
EP1167524A1 (de) 2002-01-02
DE60034878T2 (de) 2008-01-17
KR20010104720A (ko) 2001-11-26
CA2365135A1 (en) 2000-09-28
AU2944200A (en) 2000-10-09
CN1350581A (zh) 2002-05-22
EP1167524B1 (de) 2007-05-16
CA2365135C (en) 2009-08-18
CN1227357C (zh) 2005-11-16
DE60034878D1 (de) 2007-06-28
KR100682576B1 (ko) 2007-02-15
WO2000056877A1 (en) 2000-09-28
TWI259204B (en) 2006-08-01
JP3433929B2 (ja) 2003-08-04
EA004327B1 (ru) 2004-04-29
AU773536B2 (en) 2004-05-27
EP1167524A4 (de) 2005-03-09

Similar Documents

Publication Publication Date Title
DE60034878D1 (de) Verfahren zur Amplifizierung einer Nukleinsäuresequenz unter Verwendung eines chimären Primers
DK1330546T3 (da) Fremgangsmåde til amplifikation og eventuel karakterisering af nucleinsyrer
EP2272952B8 (de) Thermostabiler oder thermoactiver DNA-polymerase mit abgeschwächter 3'-5' Exonukleaseaktivität
EA200300284A1 (ru) Способ амплификации нуклеиновых кислот
EP0714987A3 (de) Verfahren zur Quantifizierung von genomischer DNA
AU6846698A (en) Method of nucleic acid amplification
DE69515029D1 (de) Nachweis von pathogenen pilzen unter verwendung der polymerase-kettenreaktion
IL137909A0 (en) Method for detection of target nucleic acids using pcr
DK0776981T3 (da) Oligonucleotidprimere til amplifikation af HCV-nukleinsyre
NO20000538L (no) Forsterkning av spesifisiteten til nukleinsyreamplifisering ved bærer nukleinsyre
EP1686191A3 (de) Fluorometrisches Verfahren zur Überwachung und zum Nachweis von Nukleinsäure- Amplifizierung
DE50013309D1 (de) Verfahren und nukleinsäuren zum nachweis von brauereirelevanten mikroorganismen
EP2180051A3 (de) Verfahren für den Nachweis und die Quantifizierung einer endogenen DNA-Sequenz aus Weizen
AU5026196A (en) Methods and reagents for typing hla class i genes
EP1548112A4 (de) Verfahren zur amplifikation von nukleinsäure, kit zur amplifikation von nukleinsäure, verfahren zum nachweis von einzelnukleotid-polymorphismus und reagentienkit zum nachweis von einzelnukleotid-polymorphismus
EP1862558A4 (de) Gennachweisverfahren
ATE320507T1 (de) Methoden zur dns vervielfältigung und material dafür
AU2002312804A1 (en) Method for detecting gram-positive bacteria having a high gc content
ES2129365A1 (es) Metodo para la deteccion de secuencias especificas de acidos nucleicos en presencia de un vector util como control positivo interno.
WO2001023611A3 (en) Methods for detecting amplification by change of fluorescence

Legal Events

Date Code Title Description
RER Ceased as to paragraph 5 lit. 3 law introducing patent treaties