ATE363530T1 - Enzymstabilisierung durch kationische, oberflächenaktive substanzen - Google Patents
Enzymstabilisierung durch kationische, oberflächenaktive substanzenInfo
- Publication number
- ATE363530T1 ATE363530T1 AT99931875T AT99931875T ATE363530T1 AT E363530 T1 ATE363530 T1 AT E363530T1 AT 99931875 T AT99931875 T AT 99931875T AT 99931875 T AT99931875 T AT 99931875T AT E363530 T1 ATE363530 T1 AT E363530T1
- Authority
- AT
- Austria
- Prior art keywords
- cationic
- active substances
- enzyme stabilization
- stabilization
- stabilize
- Prior art date
Links
- 102000004190 Enzymes Human genes 0.000 title abstract 3
- 108090000790 Enzymes Proteins 0.000 title abstract 3
- 230000006641 stabilisation Effects 0.000 title abstract 2
- 238000011105 stabilization Methods 0.000 title abstract 2
- 239000013543 active substance Substances 0.000 title 1
- 102000016928 DNA-directed DNA polymerase Human genes 0.000 abstract 2
- 108010014303 DNA-directed DNA polymerase Proteins 0.000 abstract 2
- 239000003093 cationic surfactant Substances 0.000 abstract 2
- 102100034343 Integrase Human genes 0.000 abstract 1
- 108010092799 RNA-directed DNA polymerase Proteins 0.000 abstract 1
- 150000001412 amines Chemical class 0.000 abstract 1
- 239000007864 aqueous solution Substances 0.000 abstract 1
- 238000000034 method Methods 0.000 abstract 1
- 239000000203 mixture Substances 0.000 abstract 1
- 230000029983 protein stabilization Effects 0.000 abstract 1
- 239000000243 solution Substances 0.000 abstract 1
- 239000004094 surface-active agent Substances 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
- C12N9/1241—Nucleotidyltransferases (2.7.7)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
- C12N9/1241—Nucleotidyltransferases (2.7.7)
- C12N9/1252—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
- C12N9/1241—Nucleotidyltransferases (2.7.7)
- C12N9/1276—RNA-directed DNA polymerase (2.7.7.49), i.e. reverse transcriptase or telomerase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/96—Stabilising an enzyme by forming an adduct or a composition; Forming enzyme conjugates
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Enzymes And Modification Thereof (AREA)
- Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
- Detergent Compositions (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US9053998P | 1998-06-24 | 1998-06-24 | |
| US09/338,174 US6242235B1 (en) | 1998-06-24 | 1999-06-22 | Polymerase stabilization by polyethoxylated amine surfactants |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ATE363530T1 true ATE363530T1 (de) | 2007-06-15 |
Family
ID=26782391
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AT99931875T ATE363530T1 (de) | 1998-06-24 | 1999-06-23 | Enzymstabilisierung durch kationische, oberflächenaktive substanzen |
Country Status (11)
| Country | Link |
|---|---|
| US (1) | US6242235B1 (de) |
| EP (2) | EP1918368A3 (de) |
| JP (1) | JP3673175B2 (de) |
| CN (1) | CN1188518C (de) |
| AT (1) | ATE363530T1 (de) |
| AU (1) | AU761757B2 (de) |
| BR (1) | BR9911396A (de) |
| CA (1) | CA2335153C (de) |
| DE (1) | DE69936201T2 (de) |
| ES (1) | ES2288317T3 (de) |
| WO (1) | WO1999067371A1 (de) |
Families Citing this family (47)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE19520398B4 (de) | 1995-06-08 | 2009-04-16 | Roche Diagnostics Gmbh | Magnetisches Pigment |
| KR100463475B1 (ko) | 1995-06-08 | 2005-06-22 | 로셰 디아그노스틱스 게엠베하 | 자기성피그먼트 |
| DE19743518A1 (de) | 1997-10-01 | 1999-04-15 | Roche Diagnostics Gmbh | Automatisierbare universell anwendbare Probenvorbereitungsmethode |
| US6632645B1 (en) | 2000-03-02 | 2003-10-14 | Promega Corporation | Thermophilic DNA polymerases from Thermoactinomyces vulgaris |
| US6617136B2 (en) | 2001-04-24 | 2003-09-09 | 3M Innovative Properties Company | Biological sample processing methods and compositions that include surfactants |
| US20030134292A1 (en) * | 2001-10-30 | 2003-07-17 | Farchaus Joseph W. | Thermostable DNA polymerases and methods of making same |
| US6814845B2 (en) * | 2001-11-21 | 2004-11-09 | University Of Kansas | Method for depositing an enzyme on an electrically conductive substrate |
| EP2345738A1 (de) * | 2002-08-05 | 2011-07-20 | Quanta Biosciences, Inc. | Verbesserte Zusammensetzung zur In-vitro-Amplifikation von Nukleinsäuren |
| EP1558748A4 (de) * | 2002-11-01 | 2008-10-22 | Promega Corp | Zellysezusammensetzungen, verwendungsverfahren, vorrichtung und kit |
| AU2006287548A1 (en) * | 2005-09-06 | 2007-03-15 | Gen-Probe Incorporated | Methods, compositions and kits for isothermal amplification of nucleic acids |
| EP2210955A3 (de) * | 2006-05-23 | 2010-09-15 | Molecular Detection, Inc. | Bei Umgebungstemperatur beständige Kits zur Molekulardiagnostik |
| JP5479895B2 (ja) * | 2006-07-25 | 2014-04-23 | アジレント・テクノロジーズ・インク | Ｄｎａポリメラーゼの保存及び使用のための両性イオン洗剤 |
| US7846703B2 (en) * | 2006-10-02 | 2010-12-07 | Takara Bio Inc. | Method for enhancing polymerase activity |
| WO2008045288A2 (en) * | 2006-10-06 | 2008-04-17 | Vandalia Research, Inc. | Method for a continuous rapid thermo cycle system |
| US7972828B2 (en) * | 2006-12-19 | 2011-07-05 | Sigma-Aldrich Co. | Stabilized compositions of thermostable DNA polymerase and anionic or zwitterionic detergent |
| EP1970440A1 (de) * | 2007-03-06 | 2008-09-17 | Qiagen GmbH | Polymerasestabilisierung durch ionische Reinigungsmittel |
| CN101679957A (zh) * | 2007-06-13 | 2010-03-24 | 通用电气医疗集团生物科学公司 | 聚合酶稳定 |
| WO2009006301A2 (en) * | 2007-06-29 | 2009-01-08 | Battelle Memorial Institute | Protein stabilization |
| EP2075331A1 (de) * | 2007-12-28 | 2009-07-01 | Qiagen GmbH | Modifizierte Enzyme und deren Verwendung |
| WO2009098485A1 (en) * | 2008-02-07 | 2009-08-13 | Forensic Sciences Service Ltd | Improvements in and relating to analysis |
| US20120111727A1 (en) * | 2009-01-26 | 2012-05-10 | Vladislav Dolnik | Capillary sieving electrophoresis with a cationic surfactant for size separation of proteins |
| US20100267092A1 (en) * | 2009-02-09 | 2010-10-21 | Frederic Zenhausern | Components |
| GB0915796D0 (en) | 2009-09-09 | 2009-10-07 | Fermentas Uab | Polymerase compositions and uses |
| CN102051352B (zh) * | 2009-11-02 | 2012-07-04 | 刘萍 | 一种酶结合物稳定液 |
| US9376709B2 (en) | 2010-07-26 | 2016-06-28 | Biomatrica, Inc. | Compositions for stabilizing DNA and RNA in blood and other biological samples during shipping and storage at ambient temperatures |
| EP2598661B1 (de) | 2010-07-26 | 2017-09-27 | Biomatrica, INC. | Zusammensetzungen zur stabilisierung von dna, rna und proteinen in speichel- und anderen biologischen proben während des transports und der lagerung bei umgebungstemperaturen |
| US8715987B2 (en) | 2011-05-02 | 2014-05-06 | New England Biolabs, Inc. | Solubilized phospholipids for stabilizing nucleic acid polymerases |
| EP3461807B1 (de) | 2011-06-08 | 2023-07-12 | Life Technologies Corporation | Design und entwicklung neuartiger reinigungsmittel zur verwendung in pcr-systemen |
| US9567628B2 (en) | 2011-06-08 | 2017-02-14 | Life Technologies Corporation | Polymerization of nucleic acids using proteins having low isoelectric points |
| MX345557B (es) | 2011-07-06 | 2017-02-02 | Quest Diagnostics Invest Inc | Amplificacion y deteccion directa de patogenos virales y bacteriales. |
| CN102559647A (zh) * | 2011-12-31 | 2012-07-11 | 湖南利尔康生物有限公司 | 提高液体酶应用效果的方法 |
| JP6333737B2 (ja) | 2012-02-09 | 2018-05-30 | ライフ テクノロジーズ コーポレーション | コンジュゲートされたポリマー粒子およびその製造方法 |
| US8956816B2 (en) | 2012-06-05 | 2015-02-17 | Pacific Biosciences Of California, Inc. | Methods and compositions for performing analytical operations |
| US9085761B1 (en) | 2012-06-14 | 2015-07-21 | Affymetrix, Inc. | Methods and compositions for amplification of nucleic acids |
| US9725703B2 (en) | 2012-12-20 | 2017-08-08 | Biomatrica, Inc. | Formulations and methods for stabilizing PCR reagents |
| JP6742238B2 (ja) | 2013-10-25 | 2020-08-19 | ライフ テクノロジーズ コーポレーション | Ｐｃｒシステムにおいて使用するための新規化合物及びその用途 |
| JP2015188343A (ja) * | 2014-03-27 | 2015-11-02 | セイコーエプソン株式会社 | バイオチップ |
| WO2015191632A1 (en) | 2014-06-10 | 2015-12-17 | Biomatrica, Inc. | Stabilization of thrombocytes at ambient temperatures |
| WO2016014493A1 (en) * | 2014-07-22 | 2016-01-28 | Bio-Rad Laboratories, Inc. | Buffers for use with polymerases |
| EP3317426B1 (de) | 2015-07-02 | 2020-01-15 | Life Technologies Corporation | Konjugation von funktionellen hydrophilen carboxylkügelchen |
| WO2017007774A1 (en) | 2015-07-06 | 2017-01-12 | Life Technologies Corporation | Substrates and methods useful in sequencing |
| ES2946184T3 (es) | 2015-12-08 | 2023-07-13 | Biomatrica Inc | Reducción de la velocidad de eritrosedimentación |
| LT3402880T (lt) | 2016-01-15 | 2024-03-12 | Thermo Fisher Scientific Baltics Uab | Termofiliniai dnr polimerazės mutantai |
| US11618891B2 (en) | 2017-06-26 | 2023-04-04 | Thermo Fisher Scientific Baltics Uab | Thermophilic DNA polymerase mutants |
| EP3665278A1 (de) * | 2017-08-08 | 2020-06-17 | Thermo Fisher Scientific Baltics UAB | Glycerinfreie formulierungen für reverse-transkriptasen |
| DE112017008224B4 (de) | 2017-11-21 | 2023-12-14 | Nanohelix Co., Ltd. | Zusammensetzung für polymerasereaktion |
| US11512268B2 (en) * | 2017-11-29 | 2022-11-29 | Basf Se | Storage-stable enzyme preparations, their production and use |
Family Cites Families (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB1604249A (en) * | 1977-05-31 | 1981-12-02 | Kolehmainen S | Selective measurement of somatic and microbial cells |
| US4683195A (en) | 1986-01-30 | 1987-07-28 | Cetus Corporation | Process for amplifying, detecting, and/or-cloning nucleic acid sequences |
| US4965188A (en) | 1986-08-22 | 1990-10-23 | Cetus Corporation | Process for amplifying, detecting, and/or cloning nucleic acid sequences using a thermostable enzyme |
| US4683202A (en) | 1985-03-28 | 1987-07-28 | Cetus Corporation | Process for amplifying nucleic acid sequences |
| CA1338457C (en) | 1986-08-22 | 1996-07-16 | Henry A. Erlich | Purified thermostable enzyme |
| US5352600A (en) | 1986-08-22 | 1994-10-04 | Hoffmann-La Roche Inc. | Purified thermostable enzyme |
| EP0295859B1 (de) * | 1987-06-15 | 1994-11-17 | Southern Cross Biotech Pty.Ltd. | Herstellung von Proteinen in aktiver Form |
| USRE34157E (en) * | 1988-06-23 | 1993-01-05 | S. C. Johnson & Son, Inc. | Hairspray with improved adhesion/removability upon washing |
| US5210036A (en) | 1990-04-26 | 1993-05-11 | New England Biolabs, Inc. | Purified thermostable DNA polymerase obtainable from thermococcus litoralis |
| US5356800A (en) * | 1992-11-30 | 1994-10-18 | Buckman Laboratories International, Inc. | Stabilized liquid enzymatic compositions |
-
1999
- 1999-06-22 US US09/338,174 patent/US6242235B1/en not_active Expired - Lifetime
- 1999-06-23 AU AU48293/99A patent/AU761757B2/en not_active Expired
- 1999-06-23 AT AT99931875T patent/ATE363530T1/de active
- 1999-06-23 CN CNB998088617A patent/CN1188518C/zh not_active Expired - Lifetime
- 1999-06-23 CA CA002335153A patent/CA2335153C/en not_active Expired - Lifetime
- 1999-06-23 ES ES99931875T patent/ES2288317T3/es not_active Expired - Lifetime
- 1999-06-23 BR BR9911396-1A patent/BR9911396A/pt not_active Application Discontinuation
- 1999-06-23 EP EP07010583A patent/EP1918368A3/de not_active Withdrawn
- 1999-06-23 DE DE69936201T patent/DE69936201T2/de not_active Expired - Lifetime
- 1999-06-23 JP JP2000556016A patent/JP3673175B2/ja not_active Expired - Lifetime
- 1999-06-23 WO PCT/US1999/014217 patent/WO1999067371A1/en not_active Ceased
- 1999-06-23 EP EP99931875A patent/EP1088060B1/de not_active Expired - Lifetime
Also Published As
| Publication number | Publication date |
|---|---|
| US6242235B1 (en) | 2001-06-05 |
| EP1918368A2 (de) | 2008-05-07 |
| JP2004500005A (ja) | 2004-01-08 |
| BR9911396A (pt) | 2002-01-29 |
| AU761757B2 (en) | 2003-06-12 |
| CN1315996A (zh) | 2001-10-03 |
| DE69936201T2 (de) | 2008-02-07 |
| CN1188518C (zh) | 2005-02-09 |
| ES2288317T3 (es) | 2008-01-01 |
| CA2335153A1 (en) | 1999-12-29 |
| EP1088060A4 (de) | 2004-12-01 |
| DE69936201D1 (de) | 2007-07-12 |
| EP1918368A3 (de) | 2008-06-18 |
| AU4829399A (en) | 2000-01-10 |
| CA2335153C (en) | 2007-04-10 |
| EP1088060B1 (de) | 2007-05-30 |
| EP1088060A1 (de) | 2001-04-04 |
| HK1040262A1 (en) | 2002-05-31 |
| WO1999067371A1 (en) | 1999-12-29 |
| JP3673175B2 (ja) | 2005-07-20 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| UEP | Publication of translation of european patent specification |
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