ATE368129T1 - Verfahren unter verwendung ungleicher primer- konzentrationen zur herstellung von nukleinsäure- amplifikaten - Google Patents

Verfahren unter verwendung ungleicher primer- konzentrationen zur herstellung von nukleinsäure- amplifikaten

Info

Publication number
ATE368129T1
ATE368129T1 AT99924172T AT99924172T ATE368129T1 AT E368129 T1 ATE368129 T1 AT E368129T1 AT 99924172 T AT99924172 T AT 99924172T AT 99924172 T AT99924172 T AT 99924172T AT E368129 T1 ATE368129 T1 AT E368129T1
Authority
AT
Austria
Prior art keywords
primer
target sequence
nucleic acid
primer concentrations
producing nucleic
Prior art date
Application number
AT99924172T
Other languages
English (en)
Inventor
John Salituro
John Carrino
Original Assignee
Abbott Lab
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Abbott Lab filed Critical Abbott Lab
Application granted granted Critical
Publication of ATE368129T1 publication Critical patent/ATE368129T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
AT99924172T 1998-05-15 1999-05-11 Verfahren unter verwendung ungleicher primer- konzentrationen zur herstellung von nukleinsäure- amplifikaten ATE368129T1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US09/079,675 US6391544B1 (en) 1998-05-15 1998-05-15 Method for using unequal primer concentrations for generating nucleic acid amplification products

Publications (1)

Publication Number Publication Date
ATE368129T1 true ATE368129T1 (de) 2007-08-15

Family

ID=22152084

Family Applications (1)

Application Number Title Priority Date Filing Date
AT99924172T ATE368129T1 (de) 1998-05-15 1999-05-11 Verfahren unter verwendung ungleicher primer- konzentrationen zur herstellung von nukleinsäure- amplifikaten

Country Status (7)

Country Link
US (2) US6391544B1 (de)
EP (1) EP1076721B1 (de)
JP (1) JP4634608B2 (de)
AT (1) ATE368129T1 (de)
CA (1) CA2332518C (de)
DE (1) DE69936642T2 (de)
WO (1) WO1999060159A2 (de)

Families Citing this family (37)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6391544B1 (en) * 1998-05-15 2002-05-21 Abbott Laboratories Method for using unequal primer concentrations for generating nucleic acid amplification products
US7115365B2 (en) * 1998-12-07 2006-10-03 Olympus Optical Co., Ltd. Method of analyzing a target nucleic acid
JP4824236B2 (ja) * 1999-07-09 2011-11-30 ジェン−プローブ・インコーポレーテッド 核酸増幅によるｈｉｖ−１の検出
US6852491B2 (en) * 2001-09-04 2005-02-08 Abbott Laboratories Amplification and detection reagents for HIV-1
US7198897B2 (en) * 2001-12-19 2007-04-03 Brandeis University Late-PCR
WO2004068112A2 (en) * 2003-01-28 2004-08-12 Gorilla Genomics, Inc. Hairpin primer amplification
WO2005067646A2 (en) * 2004-01-07 2005-07-28 Hitachi Chemical Research Center, Inc. Primers and probes for the detection of hiv
AU2005216549A1 (en) * 2004-02-27 2005-09-09 President And Fellows Of Harvard College Polony fluorescent in situ sequencing beads
US20090068650A1 (en) * 2005-02-11 2009-03-12 Southern Illinois University Metabolic Primers for the Detection of (Per) Chlorate-Reducing Bacteria and Methods of Use Thereof
US7700756B2 (en) * 2006-07-27 2010-04-20 Southern Illinois University Metabolic primers for the detection of perchlorate-reducing bacteria and methods of use thereof
EP2542698B1 (de) 2010-03-03 2015-04-22 Zymo Research Corporation Erkennung einer dna-methylierung
GB2497912B (en) 2010-10-08 2014-06-04 Harvard College High-throughput single cell barcoding
CA2814047C (en) 2010-10-08 2017-11-14 President And Fellows Of Harvard College High-throughput immune sequencing
US8409807B2 (en) 2010-10-22 2013-04-02 T2 Biosystems, Inc. NMR systems and methods for the rapid detection of analytes
CA2815085C (en) 2010-10-22 2022-06-21 T2 Biosystems, Inc. Nmr systems and methods for the rapid detection of analytes
US8563298B2 (en) 2010-10-22 2013-10-22 T2 Biosystems, Inc. NMR systems and methods for the rapid detection of analytes
EP3604555B1 (de) 2011-10-14 2024-12-25 President and Fellows of Harvard College Sequenzierung durch strukturanordnung
US10202628B2 (en) 2012-02-17 2019-02-12 President And Fellows Of Harvard College Assembly of nucleic acid sequences in emulsions
EP2839038B1 (de) 2012-04-20 2019-01-16 T2 Biosystems, Inc. Zusammensetzungen und verfahren für den nachweis einer candida-spezies
US9914967B2 (en) 2012-06-05 2018-03-13 President And Fellows Of Harvard College Spatial sequencing of nucleic acids using DNA origami probes
EP2875458A2 (de) 2012-07-19 2015-05-27 President and Fellows of Harvard College Verfahren zur speicherung von informationen mithilfe von nukleinsäuren
US9476089B2 (en) 2012-10-18 2016-10-25 President And Fellows Of Harvard College Methods of making oligonucleotide probes
EP2971184B1 (de) 2013-03-12 2019-04-17 President and Fellows of Harvard College Verfahren zur erzeugung einer dreidimensionalen nukleinsäure mit matrix
US9765404B2 (en) * 2013-09-04 2017-09-19 Dow Agrosciences Llc Rapid assay for identifying transformants having targeted donor insertion
CA2940322C (en) 2014-02-21 2021-05-18 President And Fellows Of Harvard College De novo design of allosteric proteins
TWI638047B (zh) 2014-04-09 2018-10-11 史基普研究協會 藉由核酸三磷酸酯轉運子將非天然或經修飾的核苷三磷酸酯輸入至細胞中
EP3218467A4 (de) 2014-11-13 2018-04-11 The Board of Trustees of the University of Illinois Biologisch manipulierte hyperfunktionale »super -helikasen
EP3640341B1 (de) 2015-02-24 2022-07-27 Zymo Research Corporation Tests zur bestimmung von dna-methylierung und dna-methylierungsmarkern von krebs
DE112016003948T5 (de) 2015-08-31 2018-05-09 City Of Sapporo Molekulare verfahren zum beurteilen einer urothelialen erkrankung
JP7523776B2 (ja) 2016-01-21 2024-07-29 ティー２ バイオシステムズ，インコーポレーテッド 細菌を迅速に検出するｎｍｒ法及びシステム
WO2018213803A1 (en) 2017-05-19 2018-11-22 Neon Therapeutics, Inc. Immunogenic neoantigen identification
JP7325341B2 (ja) 2017-07-11 2023-08-14 シンソークス，インク． 非天然ヌクレオチドの組み込み及びその方法
KR20240141853A (ko) 2017-08-03 2024-09-27 신톡스, 인크. 자가면역 질환의 치료를 위한 사이토카인 접합체
EP3613855A1 (de) 2018-08-23 2020-02-26 Clariant Produkte (Deutschland) GmbH Verfahren zur herstellung einer nukleinsäurenbibliothek
US20210388431A1 (en) * 2018-10-29 2021-12-16 Igor V. Kutyavin Thermokinetically balanced isothermal amplification of nucleic acid sequences
EP3923974A4 (de) 2019-02-06 2023-02-08 Synthorx, Inc. Il-2-konjugate und verfahren zur verwendung davon
CN119968468A (zh) 2022-07-12 2025-05-09 拓扑基因股份有限公司 Dna芯片亚微米精度大规模复制方法

Family Cites Families (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4948882A (en) 1983-02-22 1990-08-14 Syngene, Inc. Single-stranded labelled oligonucleotides, reactive monomers and methods of synthesis
US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US4683195A (en) 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
US5066584A (en) 1988-09-23 1991-11-19 Cetus Corporation Methods for generating single stranded dna by the polymerase chain reaction
US5629158A (en) 1989-03-22 1997-05-13 Cemu Bitecknik Ab Solid phase diagnosis of medical conditions
US5180820A (en) * 1989-08-30 1993-01-19 Barde Yves Alain Brain-derived neurotrophic factor
CA2024978A1 (en) 1989-09-22 1991-03-23 Annie L. Wu Nucleic acid detection method using unequal primer concentrations in polymerase chain reaction
EP0516753A4 (en) * 1990-02-26 1994-05-18 Us Health A method for the fluorescent detection of a dna sequence in real time
US5833976A (en) * 1991-08-06 1998-11-10 Schering Corporation Use of interleukin-10 (IL-10) to treat endotoxin- or superantigen-induced toxicity
ZA929351B (en) * 1991-12-11 1993-06-04 Igen Inc Electrochemiluminescent label for DNA assays.
US5424414A (en) 1991-12-17 1995-06-13 Abbott Laboratories Haptens, tracers, immunogens and antibodies for 3-phenyl-1-adamantaneacetic acids
US5464746A (en) 1991-12-17 1995-11-07 Abbott Laboratories Haptens, tracers, immunogens and antibodies for carbazole and dibenzofuran derivatives
NZ263779A (en) 1993-04-16 1997-03-24 F B Investments Pty Ltd Former Method for random amplification of dna
US5763174A (en) * 1994-02-17 1998-06-09 The Wistar Institute Of Anatomy & Biology RNA editing enzyme and methods of use thereof
US5710029A (en) * 1995-06-07 1998-01-20 Gen-Probe Incorporated Methods for determining pre-amplification levels of a nucleic acid target sequence from post-amplification levels of product
DE19808534A1 (de) 1998-02-28 1999-09-02 Roche Diagnostics Gmbh Verfahren zum Nachweis hochkonzentrierter Nukleinsäuren
US6391544B1 (en) * 1998-05-15 2002-05-21 Abbott Laboratories Method for using unequal primer concentrations for generating nucleic acid amplification products

Also Published As

Publication number Publication date
CA2332518C (en) 2009-07-14
US20020160406A1 (en) 2002-10-31
US7037688B2 (en) 2006-05-02
EP1076721A2 (de) 2001-02-21
WO1999060159A3 (en) 2000-02-24
US20020045163A1 (en) 2002-04-18
JP2002515261A (ja) 2002-05-28
DE69936642T2 (de) 2008-05-21
DE69936642D1 (de) 2007-09-06
CA2332518A1 (en) 1999-11-25
JP4634608B2 (ja) 2011-02-16
EP1076721B1 (de) 2007-07-25
US6391544B1 (en) 2002-05-21
WO1999060159A2 (en) 1999-11-25

Similar Documents

Publication Publication Date Title
ATE368129T1 (de) Verfahren unter verwendung ungleicher primer- konzentrationen zur herstellung von nukleinsäure- amplifikaten
DE3777213D1 (de)
RU2149186C1 (ru) Способ обнаружения изменения длины олигонуклеотида, меченного светоиспускающей меткой
KR870007427A (ko) 증폭 및 하이브리드화로 바이러스를 검출하는 방법
Nyrén et al. Detection of single-base changes using a bioluminometric primer extension assay
DE69837913D1 (de) Verfahren zur vervielfältigung von nukleinsäure
CA2292787A1 (en) Nucleic acid primers and probes for detecting hiv-1 and hiv-2
ATE210734T1 (de) Verfahren zur quantifizierung von genomischer dna
KR910009926A (ko) 연결 가능한 헤어핀 프로브와 전사를 이용한 핵산 증폭 방법
ATE83505T1 (de) Verfahren zur amplifikation von nukleinsaeuresequenzen.
EP3568493B1 (de) Verfahren und zusammensetzungen zur verringerung von redundanten molekularen barcodes in primerextensionsreaktionen
DE69333649D1 (de) Verbessertes Verfahren für nuklein-säure Amplifikation durch NASBA
CA2319662A1 (en) Modified nucleic acid probes and uses thereof
EP0932700A1 (de) Verfahren zur sequenzierung von dna
DE69028325D1 (de) Nukleinsäure-Amplifikation unter Verwendung eines Einzelprimers
DE69217501D1 (de) Verfahren zur feststellung der anzahl eines bestimmten dns fragments von interesse bei der enzymatischen amplifikation von dns
ATE206764T1 (de) Verfahren zum sensitiven nachweis von nukleinsäuren
BR9604814A (pt) Método para amplificar sequências de ácido nucleico especìficas
KR102098710B1 (ko) 헤어핀 프로브 기반 등온 핵산 증폭을 이용한 표적핵산 검출 방법
CA2268753A1 (en) Nucleic acid primers and probes for detecting oncogenic human papillomaviruses
AU7492894A (en) Improved method for the quantification of nucleic acid
ATE200108T1 (de) Verfahren zum nachweis von telomerase-aktivität
EP0855447A3 (de) Verfahren zur Bestimmung von Nukleinsäuresequenzen
CA2349810C (en) Isometric primer extension method and kit for detection and quantification of specific nucleic acid
CA2384917A1 (en) Nucleic acid primers and probes for detecting tumor cells

Legal Events

Date Code Title Description
RER Ceased as to paragraph 5 lit. 3 law introducing patent treaties