ATE368129T1 - Verfahren unter verwendung ungleicher primer- konzentrationen zur herstellung von nukleinsäure- amplifikaten - Google Patents

Verfahren unter verwendung ungleicher primer- konzentrationen zur herstellung von nukleinsäure- amplifikaten

Info

Publication number
ATE368129T1
ATE368129T1 AT99924172T AT99924172T ATE368129T1 AT E368129 T1 ATE368129 T1 AT E368129T1 AT 99924172 T AT99924172 T AT 99924172T AT 99924172 T AT99924172 T AT 99924172T AT E368129 T1 ATE368129 T1 AT E368129T1
Authority
AT
Austria
Prior art keywords
primer
target sequence
nucleic acid
primer concentrations
producing nucleic
Prior art date
Application number
AT99924172T
Other languages
English (en)
Inventor
John Salituro
John Carrino
Original Assignee
Abbott Lab
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Abbott Lab filed Critical Abbott Lab
Application granted granted Critical
Publication of ATE368129T1 publication Critical patent/ATE368129T1/de

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
AT99924172T 1998-05-15 1999-05-11 Verfahren unter verwendung ungleicher primer- konzentrationen zur herstellung von nukleinsäure- amplifikaten ATE368129T1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US09/079,675 US6391544B1 (en) 1998-05-15 1998-05-15 Method for using unequal primer concentrations for generating nucleic acid amplification products

Publications (1)

Publication Number Publication Date
ATE368129T1 true ATE368129T1 (de) 2007-08-15

Family

ID=22152084

Family Applications (1)

Application Number Title Priority Date Filing Date
AT99924172T ATE368129T1 (de) 1998-05-15 1999-05-11 Verfahren unter verwendung ungleicher primer- konzentrationen zur herstellung von nukleinsäure- amplifikaten

Country Status (7)

Country Link
US (2) US6391544B1 (de)
EP (1) EP1076721B1 (de)
JP (1) JP4634608B2 (de)
AT (1) ATE368129T1 (de)
CA (1) CA2332518C (de)
DE (1) DE69936642T2 (de)
WO (1) WO1999060159A2 (de)

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US6852491B2 (en) * 2001-09-04 2005-02-08 Abbott Laboratories Amplification and detection reagents for HIV-1
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AU2005216549A1 (en) * 2004-02-27 2005-09-09 President And Fellows Of Harvard College Polony fluorescent in situ sequencing beads
US20090068650A1 (en) * 2005-02-11 2009-03-12 Southern Illinois University Metabolic Primers for the Detection of (Per) Chlorate-Reducing Bacteria and Methods of Use Thereof
US7700756B2 (en) * 2006-07-27 2010-04-20 Southern Illinois University Metabolic primers for the detection of perchlorate-reducing bacteria and methods of use thereof
EP2542698B1 (de) * 2010-03-03 2015-04-22 Zymo Research Corporation Erkennung einer dna-methylierung
EP2625320B1 (de) 2010-10-08 2019-03-27 President and Fellows of Harvard College Einzelzellen-barcoding mit hohem durchsatz
DK2625295T3 (da) 2010-10-08 2019-06-11 Harvard College High-throughput-immunsekvensering
CA2815085C (en) 2010-10-22 2022-06-21 T2 Biosystems, Inc. Nmr systems and methods for the rapid detection of analytes
US8409807B2 (en) 2010-10-22 2013-04-02 T2 Biosystems, Inc. NMR systems and methods for the rapid detection of analytes
US8563298B2 (en) 2010-10-22 2013-10-22 T2 Biosystems, Inc. NMR systems and methods for the rapid detection of analytes
EP3604555B1 (de) 2011-10-14 2024-12-25 President and Fellows of Harvard College Sequenzierung durch strukturanordnung
WO2014163886A1 (en) 2013-03-12 2014-10-09 President And Fellows Of Harvard College Method of generating a three-dimensional nucleic acid containing matrix
US10202628B2 (en) 2012-02-17 2019-02-12 President And Fellows Of Harvard College Assembly of nucleic acid sequences in emulsions
WO2013158281A1 (en) 2012-04-20 2013-10-24 T2 Biosystems, Inc. Compositions and methods for detection of candida species
US9914967B2 (en) 2012-06-05 2018-03-13 President And Fellows Of Harvard College Spatial sequencing of nucleic acids using DNA origami probes
EP2875458A2 (de) 2012-07-19 2015-05-27 President and Fellows of Harvard College Verfahren zur speicherung von informationen mithilfe von nukleinsäuren
US9476089B2 (en) 2012-10-18 2016-10-25 President And Fellows Of Harvard College Methods of making oligonucleotide probes
CN105682452B (zh) * 2013-09-04 2018-10-16 美国陶氏益农公司 用于在作物中确定供体插入的快速靶向分析
KR102328069B1 (ko) 2014-02-21 2021-11-17 프레지던트 앤드 펠로우즈 오브 하바드 칼리지 알로스테릭 단백질의 신생 설계
TWI638047B (zh) 2014-04-09 2018-10-11 史基普研究協會 藉由核酸三磷酸酯轉運子將非天然或經修飾的核苷三磷酸酯輸入至細胞中
WO2016077763A1 (en) 2014-11-13 2016-05-19 The Board Of Trustees Of The University Of Illinois Bio-engineered hyper-functional "super" helicases
EP3640341B1 (de) 2015-02-24 2022-07-27 Zymo Research Corporation Tests zur bestimmung von dna-methylierung und dna-methylierungsmarkern von krebs
JP6624704B2 (ja) 2015-08-31 2019-12-25 日立化成株式会社 尿路上皮疾患の評価のための分子法
AU2017210033B2 (en) 2016-01-21 2022-09-29 T2 Biosystems, Inc. NMR methods and systems for the rapid detection of bacteria
WO2018213803A1 (en) 2017-05-19 2018-11-22 Neon Therapeutics, Inc. Immunogenic neoantigen identification
SG11202000167SA (en) 2017-07-11 2020-02-27 Synthorx Inc Incorporation of unnatural nucleotides and methods thereof
KR20250145137A (ko) 2017-08-03 2025-10-13 신톡스, 인크. 자가면역 질환의 치료를 위한 사이토카인 접합체
EP3613855A1 (de) 2018-08-23 2020-02-26 Clariant Produkte (Deutschland) GmbH Verfahren zur herstellung einer nukleinsäurenbibliothek
WO2020092268A2 (en) * 2018-10-29 2020-05-07 Kutyavin Igor V Thermokinetically balanced isothermal amplification of nucleic acid sequences
US20200246467A1 (en) 2019-02-06 2020-08-06 Synthorx, Inc. Il-2 conjugates and methods of use thereof
WO2024015766A1 (en) 2022-07-12 2024-01-18 Topogene Inc. Scalable, submicron-resolution replication of dna arrays

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US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
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US5066584A (en) 1988-09-23 1991-11-19 Cetus Corporation Methods for generating single stranded dna by the polymerase chain reaction
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US5180820A (en) * 1989-08-30 1993-01-19 Barde Yves Alain Brain-derived neurotrophic factor
CA2024978A1 (en) 1989-09-22 1991-03-23 Annie L. Wu Nucleic acid detection method using unequal primer concentrations in polymerase chain reaction
AU638568B2 (en) * 1990-02-26 1993-07-01 United States of America, as represented by the Secretary, U.S. Department of Commerce, The A method for the fluorescent detection of a dna sequence in real time
US5833976A (en) * 1991-08-06 1998-11-10 Schering Corporation Use of interleukin-10 (IL-10) to treat endotoxin- or superantigen-induced toxicity
ZA929351B (en) * 1991-12-11 1993-06-04 Igen Inc Electrochemiluminescent label for DNA assays.
US5424414A (en) * 1991-12-17 1995-06-13 Abbott Laboratories Haptens, tracers, immunogens and antibodies for 3-phenyl-1-adamantaneacetic acids
US5464746A (en) * 1991-12-17 1995-11-07 Abbott Laboratories Haptens, tracers, immunogens and antibodies for carbazole and dibenzofuran derivatives
EP0695366A4 (de) 1993-04-16 1999-07-28 F B Investments Pty Ltd Methode bezüglich zufällig amplifizierter polymorpher dna (rapd)
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US5710029A (en) * 1995-06-07 1998-01-20 Gen-Probe Incorporated Methods for determining pre-amplification levels of a nucleic acid target sequence from post-amplification levels of product
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US6391544B1 (en) * 1998-05-15 2002-05-21 Abbott Laboratories Method for using unequal primer concentrations for generating nucleic acid amplification products

Also Published As

Publication number Publication date
WO1999060159A3 (en) 2000-02-24
US20020160406A1 (en) 2002-10-31
EP1076721A2 (de) 2001-02-21
CA2332518A1 (en) 1999-11-25
CA2332518C (en) 2009-07-14
US7037688B2 (en) 2006-05-02
WO1999060159A2 (en) 1999-11-25
US20020045163A1 (en) 2002-04-18
DE69936642D1 (de) 2007-09-06
US6391544B1 (en) 2002-05-21
EP1076721B1 (de) 2007-07-25
DE69936642T2 (de) 2008-05-21
JP4634608B2 (ja) 2011-02-16
JP2002515261A (ja) 2002-05-28

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