ATE368129T1 - Verfahren unter verwendung ungleicher primer- konzentrationen zur herstellung von nukleinsäure- amplifikaten - Google Patents

Verfahren unter verwendung ungleicher primer- konzentrationen zur herstellung von nukleinsäure- amplifikaten

Info

Publication number
ATE368129T1
ATE368129T1 AT99924172T AT99924172T ATE368129T1 AT E368129 T1 ATE368129 T1 AT E368129T1 AT 99924172 T AT99924172 T AT 99924172T AT 99924172 T AT99924172 T AT 99924172T AT E368129 T1 ATE368129 T1 AT E368129T1
Authority
AT
Austria
Prior art keywords
primer
target sequence
nucleic acid
primer concentrations
producing nucleic
Prior art date
Application number
AT99924172T
Other languages
English (en)
Inventor
John Salituro
John Carrino
Original Assignee
Abbott Lab
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Abbott Lab filed Critical Abbott Lab
Application granted granted Critical
Publication of ATE368129T1 publication Critical patent/ATE368129T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
AT99924172T 1998-05-15 1999-05-11 Verfahren unter verwendung ungleicher primer- konzentrationen zur herstellung von nukleinsäure- amplifikaten ATE368129T1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US09/079,675 US6391544B1 (en) 1998-05-15 1998-05-15 Method for using unequal primer concentrations for generating nucleic acid amplification products

Publications (1)

Publication Number Publication Date
ATE368129T1 true ATE368129T1 (de) 2007-08-15

Family

ID=22152084

Family Applications (1)

Application Number Title Priority Date Filing Date
AT99924172T ATE368129T1 (de) 1998-05-15 1999-05-11 Verfahren unter verwendung ungleicher primer- konzentrationen zur herstellung von nukleinsäure- amplifikaten

Country Status (7)

Country Link
US (2) US6391544B1 (de)
EP (1) EP1076721B1 (de)
JP (1) JP4634608B2 (de)
AT (1) ATE368129T1 (de)
CA (1) CA2332518C (de)
DE (1) DE69936642T2 (de)
WO (1) WO1999060159A2 (de)

Families Citing this family (37)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6391544B1 (en) * 1998-05-15 2002-05-21 Abbott Laboratories Method for using unequal primer concentrations for generating nucleic acid amplification products
US7115365B2 (en) * 1998-12-07 2006-10-03 Olympus Optical Co., Ltd. Method of analyzing a target nucleic acid
WO2001004361A2 (en) 1999-07-09 2001-01-18 Gen-Probe Incorporated Detection of hiv-1 by nucleic acid amplification
US6852491B2 (en) * 2001-09-04 2005-02-08 Abbott Laboratories Amplification and detection reagents for HIV-1
US7198897B2 (en) * 2001-12-19 2007-04-03 Brandeis University Late-PCR
US20040259116A1 (en) * 2003-01-28 2004-12-23 Gorilla Genomics, Inc. Hairpin primer amplification
CN1910289B (zh) * 2004-01-07 2012-05-23 日立化成研究中心公司 用于检测hiv的引物和探针
CN1950519A (zh) * 2004-02-27 2007-04-18 哈佛大学的校长及成员们 聚合酶群落荧光原位测序珠子
WO2006088445A2 (en) * 2005-02-11 2006-08-24 Southern Illinois University Metabolic primers for the detection of (per)chlorate-reducing bacteria and methods of use thereof
US7700756B2 (en) * 2006-07-27 2010-04-20 Southern Illinois University Metabolic primers for the detection of perchlorate-reducing bacteria and methods of use thereof
EP2542698B1 (de) * 2010-03-03 2015-04-22 Zymo Research Corporation Erkennung einer dna-methylierung
ES2730951T3 (es) 2010-10-08 2019-11-13 Harvard College Secuenciación inmune de alto rendimiento
WO2012048341A1 (en) 2010-10-08 2012-04-12 President And Fellows Of Harvard College High-throughput single cell barcoding
WO2012054639A2 (en) 2010-10-22 2012-04-26 T2 Biosystems, Inc. Nmr systems and methods for the rapid detection of analytes
US8563298B2 (en) 2010-10-22 2013-10-22 T2 Biosystems, Inc. NMR systems and methods for the rapid detection of analytes
US8409807B2 (en) 2010-10-22 2013-04-02 T2 Biosystems, Inc. NMR systems and methods for the rapid detection of analytes
EP3604555B1 (de) 2011-10-14 2024-12-25 President and Fellows of Harvard College Sequenzierung durch strukturanordnung
WO2014163886A1 (en) 2013-03-12 2014-10-09 President And Fellows Of Harvard College Method of generating a three-dimensional nucleic acid containing matrix
US10202628B2 (en) 2012-02-17 2019-02-12 President And Fellows Of Harvard College Assembly of nucleic acid sequences in emulsions
WO2013158281A1 (en) 2012-04-20 2013-10-24 T2 Biosystems, Inc. Compositions and methods for detection of candida species
WO2013184754A2 (en) 2012-06-05 2013-12-12 President And Fellows Of Harvard College Spatial sequencing of nucleic acids using dna origami probes
CN108875312A (zh) 2012-07-19 2018-11-23 哈佛大学校长及研究员协会 利用核酸存储信息的方法
US9476089B2 (en) 2012-10-18 2016-10-25 President And Fellows Of Harvard College Methods of making oligonucleotide probes
RU2668819C2 (ru) * 2013-09-04 2018-10-02 ДАУ АГРОСАЙЕНСИЗ ЭлЭлСи Быстрый направленный анализ сельскохозяйственных культур для определения донорной вставки
KR102328069B1 (ko) 2014-02-21 2021-11-17 프레지던트 앤드 펠로우즈 오브 하바드 칼리지 알로스테릭 단백질의 신생 설계
EP3129493B1 (de) 2014-04-09 2021-07-07 The Scripps Research Institute Import von unnatürlichen oder modifizierten nukleosid-triphosphaten in zellen mittels nukleinsäure-triphosphattransportern
CA2967036A1 (en) 2014-11-13 2016-05-19 The Board Of Trustees Of The University Of Illinois Bio-engineered hyper-functional "super" helicases
WO2016138105A2 (en) 2015-02-24 2016-09-01 Zymo Research Corporation Assays to determine dna methylation and dna methylation markers of cancer
US11028443B2 (en) 2015-08-31 2021-06-08 Showa Denko Materials Co., Ltd. Molecular methods for assessing urothelial disease
US11519016B2 (en) 2016-01-21 2022-12-06 T2 Biosystems, Inc. NMR methods and systems for the rapid detection of bacteria
WO2018213803A1 (en) 2017-05-19 2018-11-22 Neon Therapeutics, Inc. Immunogenic neoantigen identification
TW202426655A (zh) 2017-07-11 2024-07-01 美商新索思股份有限公司 非天然核苷酸之導入及其方法
MA49767A (fr) 2017-08-03 2021-05-26 Synthorx Inc Conjugués de cytokine pour le traitement de maladies auto-immunes
EP3613855A1 (de) 2018-08-23 2020-02-26 Clariant Produkte (Deutschland) GmbH Verfahren zur herstellung einer nukleinsäurenbibliothek
WO2020092255A1 (en) * 2018-10-29 2020-05-07 Kutyavin Igor V Accelerated polymerase chain reaction
CA3127689A1 (en) 2019-02-06 2020-08-13 Synthorx, Inc. Il-2 conjugates and methods of use thereof
EP4555102A1 (de) 2022-07-12 2025-05-21 Topogene Inc. Skalierbare replikation von dna-arrays mit submikron-auflösung

Family Cites Families (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4948882A (en) 1983-02-22 1990-08-14 Syngene, Inc. Single-stranded labelled oligonucleotides, reactive monomers and methods of synthesis
US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US4683195A (en) 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
US5066584A (en) 1988-09-23 1991-11-19 Cetus Corporation Methods for generating single stranded dna by the polymerase chain reaction
US5629158A (en) 1989-03-22 1997-05-13 Cemu Bitecknik Ab Solid phase diagnosis of medical conditions
US5180820A (en) * 1989-08-30 1993-01-19 Barde Yves Alain Brain-derived neurotrophic factor
CA2024978A1 (en) 1989-09-22 1991-03-23 Annie L. Wu Nucleic acid detection method using unequal primer concentrations in polymerase chain reaction
JPH0632639B2 (ja) * 1990-02-26 1994-05-02 アメリカ合衆国 Ｄｎａ配列を即時に蛍光検出する方法
US5833976A (en) * 1991-08-06 1998-11-10 Schering Corporation Use of interleukin-10 (IL-10) to treat endotoxin- or superantigen-induced toxicity
ZA929351B (en) * 1991-12-11 1993-06-04 Igen Inc Electrochemiluminescent label for DNA assays.
US5424414A (en) 1991-12-17 1995-06-13 Abbott Laboratories Haptens, tracers, immunogens and antibodies for 3-phenyl-1-adamantaneacetic acids
US5464746A (en) 1991-12-17 1995-11-07 Abbott Laboratories Haptens, tracers, immunogens and antibodies for carbazole and dibenzofuran derivatives
US5759821A (en) 1993-04-16 1998-06-02 F B Investments Pty Ltd Method of random amplification of polymorphic DNA
US5763174A (en) * 1994-02-17 1998-06-09 The Wistar Institute Of Anatomy & Biology RNA editing enzyme and methods of use thereof
US5710029A (en) * 1995-06-07 1998-01-20 Gen-Probe Incorporated Methods for determining pre-amplification levels of a nucleic acid target sequence from post-amplification levels of product
DE19808534A1 (de) 1998-02-28 1999-09-02 Roche Diagnostics Gmbh Verfahren zum Nachweis hochkonzentrierter Nukleinsäuren
US6391544B1 (en) * 1998-05-15 2002-05-21 Abbott Laboratories Method for using unequal primer concentrations for generating nucleic acid amplification products

Also Published As

Publication number Publication date
US6391544B1 (en) 2002-05-21
JP2002515261A (ja) 2002-05-28
US20020045163A1 (en) 2002-04-18
EP1076721B1 (de) 2007-07-25
US7037688B2 (en) 2006-05-02
CA2332518A1 (en) 1999-11-25
WO1999060159A3 (en) 2000-02-24
WO1999060159A2 (en) 1999-11-25
DE69936642T2 (de) 2008-05-21
US20020160406A1 (en) 2002-10-31
EP1076721A2 (de) 2001-02-21
CA2332518C (en) 2009-07-14
DE69936642D1 (de) 2007-09-06
JP4634608B2 (ja) 2011-02-16

Similar Documents

Publication Publication Date Title
ATE368129T1 (de) Verfahren unter verwendung ungleicher primer- konzentrationen zur herstellung von nukleinsäure- amplifikaten
DE3777213D1 (de)
RU2149186C1 (ru) Способ обнаружения изменения длины олигонуклеотида, меченного светоиспускающей меткой
US5849547A (en) Method for nucleic acid amplification by transcription using displacement, and reagents and kit therefor
KR870007427A (ko) 증폭 및 하이브리드화로 바이러스를 검출하는 방법
Nyrén et al. Detection of single-base changes using a bioluminometric primer extension assay
DE69837913D1 (de) Verfahren zur vervielfältigung von nukleinsäure
CA2292787A1 (en) Nucleic acid primers and probes for detecting hiv-1 and hiv-2
DE59509937D1 (de) Verfahren zur Quantifizierung von genomischer DNA
KR910009926A (ko) 연결 가능한 헤어핀 프로브와 전사를 이용한 핵산 증폭 방법
ATE83505T1 (de) Verfahren zur amplifikation von nukleinsaeuresequenzen.
EP3568493B1 (de) Verfahren und zusammensetzungen zur verringerung von redundanten molekularen barcodes in primerextensionsreaktionen
HUP0101385A2 (hu) Eljárás nukleinsavak detektálására PCR-módszerrel
DE69333649D1 (de) Verbessertes Verfahren für nuklein-säure Amplifikation durch NASBA
CA2319662A1 (en) Modified nucleic acid probes and uses thereof
WO1998013523A1 (en) Method of sequencing dna
DE69028325D1 (de) Nukleinsäure-Amplifikation unter Verwendung eines Einzelprimers
DE69217501D1 (de) Verfahren zur feststellung der anzahl eines bestimmten dns fragments von interesse bei der enzymatischen amplifikation von dns
ATE206764T1 (de) Verfahren zum sensitiven nachweis von nukleinsäuren
BR9604814A (pt) Método para amplificar sequências de ácido nucleico especìficas
KR102098710B1 (ko) 헤어핀 프로브 기반 등온 핵산 증폭을 이용한 표적핵산 검출 방법
AU7492894A (en) Improved method for the quantification of nucleic acid
ATE200108T1 (de) Verfahren zum nachweis von telomerase-aktivität
EP0855447A3 (de) Verfahren zur Bestimmung von Nukleinsäuresequenzen
CA2349810C (en) Isometric primer extension method and kit for detection and quantification of specific nucleic acid

Legal Events

Date Code Title Description
RER Ceased as to paragraph 5 lit. 3 law introducing patent treaties