ATE371731T1 - Verfahren zur skalierbaren reinigung von plasmid- dns - Google Patents

Verfahren zur skalierbaren reinigung von plasmid- dns

Info

Publication number
ATE371731T1
ATE371731T1 AT00989348T AT00989348T ATE371731T1 AT E371731 T1 ATE371731 T1 AT E371731T1 AT 00989348 T AT00989348 T AT 00989348T AT 00989348 T AT00989348 T AT 00989348T AT E371731 T1 ATE371731 T1 AT E371731T1
Authority
AT
Austria
Prior art keywords
plasmid dna
purification
scalable purification
clinical grade
dna
Prior art date
Application number
AT00989348T
Other languages
English (en)
Inventor
Russel Lander
Michael Winters
Francis Meacle
Original Assignee
Merck & Co Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Merck & Co Inc filed Critical Merck & Co Inc
Application granted granted Critical
Publication of ATE371731T1 publication Critical patent/ATE371731T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Saccharide Compounds (AREA)
  • Peptides Or Proteins (AREA)
  • Solid-Sorbent Or Filter-Aiding Compositions (AREA)
AT00989348T 1999-12-22 2000-12-21 Verfahren zur skalierbaren reinigung von plasmid- dns ATE371731T1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US17147299P 1999-12-22 1999-12-22

Publications (1)

Publication Number Publication Date
ATE371731T1 true ATE371731T1 (de) 2007-09-15

Family

ID=22623856

Family Applications (1)

Application Number Title Priority Date Filing Date
AT00989348T ATE371731T1 (de) 1999-12-22 2000-12-21 Verfahren zur skalierbaren reinigung von plasmid- dns

Country Status (12)

Country Link
US (1) US20010044136A1 (de)
EP (1) EP1242442B1 (de)
JP (1) JP4856833B2 (de)
AT (1) ATE371731T1 (de)
AU (1) AU781200B2 (de)
CA (1) CA2393479C (de)
CY (1) CY1107777T1 (de)
DE (1) DE60036226T2 (de)
DK (1) DK1242442T3 (de)
ES (1) ES2291233T3 (de)
PT (1) PT1242442E (de)
WO (1) WO2001046215A1 (de)

Families Citing this family (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6995246B1 (en) * 2000-10-19 2006-02-07 Akzo Nobel N.V. Methods for removing suspended particles from soluble protein solutions
JP4723254B2 (ja) * 2002-12-23 2011-07-13 バイカル インコーポレイテッド プラスミドｄｎａを精製するための方法
AU2006211216B2 (en) 2005-01-31 2011-02-03 Merck Sharp & Dohme Llc Purification process for plasmid DNA
US20060223072A1 (en) * 2005-03-31 2006-10-05 Boyes Barry E Methods of using a DNase I-like enzyme
US20060223073A1 (en) * 2005-03-31 2006-10-05 Boyes Barry E Methods of using a DNase I-like enzyme
US20060270843A1 (en) * 2005-05-26 2006-11-30 Hall Gerald E Jr Methods for isolation of nucleic acids
JP5793726B2 (ja) * 2007-05-23 2015-10-14 ブイジーエックスアイ，インコーポレイティド 高濃度の生物学的に活性な分子を含む組成物とその製造方法
US20090227011A1 (en) * 2008-02-29 2009-09-10 Shizhong Chen Methods of purifying plasmid dna
CA2965500A1 (en) * 2014-10-24 2016-04-28 Abbott Molecular Inc. Enrichment of small nucleic acids
CN105002160B (zh) * 2015-07-15 2018-01-12 澳门科技大学 中成药或含中药材保健品的dna的提取方法
MX2020012910A (es) * 2018-05-31 2021-05-27 Vgxi Inc Aparato de espiral de lisis y usos del mismo para el aislamiento y purificacion de polinucleotidos.
WO2025252480A1 (en) 2024-06-07 2025-12-11 F. Hoffmann-La Roche Ag Method for purifying plasmid dna

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DK0915906T3 (da) * 1996-07-10 2000-11-20 American Nat Red Cross Fremgangsmåder til selektiv adskillelse af organiske komponenter fra biologiske væsker
CA2214495C (en) * 1996-09-25 2002-02-05 Daniel L. Woodard Hydrated zirconium silicate composition for purification of nucleic acids

Also Published As

Publication number Publication date
PT1242442E (pt) 2007-11-08
DK1242442T3 (da) 2007-12-17
AU2585901A (en) 2001-07-03
ES2291233T3 (es) 2008-03-01
CA2393479C (en) 2010-10-05
CY1107777T1 (el) 2013-06-19
CA2393479A1 (en) 2001-06-28
EP1242442A1 (de) 2002-09-25
DE60036226T2 (de) 2008-05-21
AU781200B2 (en) 2005-05-12
DE60036226D1 (de) 2007-10-11
JP4856833B2 (ja) 2012-01-18
US20010044136A1 (en) 2001-11-22
WO2001046215A1 (en) 2001-06-28
EP1242442B1 (de) 2007-08-29
JP2004504006A (ja) 2004-02-12
EP1242442A4 (de) 2005-02-16

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