ATE372381T1 - Bedingtes 'gene trapping'-konstrukt zur unterbrechung von genen - Google Patents

Bedingtes 'gene trapping'-konstrukt zur unterbrechung von genen

Info

Publication number
ATE372381T1
ATE372381T1 AT00974397T AT00974397T ATE372381T1 AT E372381 T1 ATE372381 T1 AT E372381T1 AT 00974397 T AT00974397 T AT 00974397T AT 00974397 T AT00974397 T AT 00974397T AT E372381 T1 ATE372381 T1 AT E372381T1
Authority
AT
Austria
Prior art keywords
genes
construct
gene trapping
relates
cell
Prior art date
Application number
AT00974397T
Other languages
English (en)
Inventor
Ralf Kuehn
Melchener Harald Von
Joachim Altschmied
Original Assignee
Artemis Pharmaceuticals Gmbh
Frankgen Biotechnologie Ag
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Artemis Pharmaceuticals Gmbh, Frankgen Biotechnologie Ag filed Critical Artemis Pharmaceuticals Gmbh
Application granted granted Critical
Publication of ATE372381T1 publication Critical patent/ATE372381T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2217/00—Genetically modified animals
    • A01K2217/05—Animals comprising random inserted nucleic acids (transgenic)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2800/00—Nucleic acids vectors
    • C12N2800/30—Vector systems comprising sequences for excision in presence of a recombinase, e.g. loxP or FRT
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2800/00—Nucleic acids vectors
    • C12N2800/60—Vectors containing traps for, e.g. exons, promoters
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2840/00—Vectors comprising a special translation-regulating system
    • C12N2840/20—Vectors comprising a special translation-regulating system translation of more than one cistron
    • C12N2840/203—Vectors comprising a special translation-regulating system translation of more than one cistron having an IRES
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2840/00—Vectors comprising a special translation-regulating system
    • C12N2840/44—Vectors comprising a special translation-regulating system being a specific part of the splice mechanism, e.g. donor, acceptor

Landscapes

  • Genetics & Genomics (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Mycology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
AT00974397T 1999-10-16 2000-10-16 Bedingtes 'gene trapping'-konstrukt zur unterbrechung von genen ATE372381T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP99120592A EP1092768A1 (de) 1999-10-16 1999-10-16 Konstrukt zum bedingten einfangen und Disruption von Genen
US16201699P 1999-10-27 1999-10-27

Publications (1)

Publication Number Publication Date
ATE372381T1 true ATE372381T1 (de) 2007-09-15

Family

ID=8239213

Family Applications (1)

Application Number Title Priority Date Filing Date
AT00974397T ATE372381T1 (de) 1999-10-16 2000-10-16 Bedingtes 'gene trapping'-konstrukt zur unterbrechung von genen

Country Status (8)

Country Link
EP (2) EP1092768A1 (de)
JP (1) JP2003512053A (de)
AT (1) ATE372381T1 (de)
AU (1) AU782960B2 (de)
CA (1) CA2387737A1 (de)
DE (1) DE60036297T2 (de)
ES (1) ES2295063T3 (de)
WO (1) WO2001029208A1 (de)

Families Citing this family (21)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7449179B2 (en) 2000-11-16 2008-11-11 Cornell Research Foundation, Inc. Vectors for conditional gene inactivation
WO2003015505A2 (en) * 2001-08-14 2003-02-27 Frankgen Biotechnologie Ag An animal model exhibiting cancer, pulmonary emphysema and cardiomyopathy
DE10140030C1 (de) * 2001-08-16 2002-12-19 Gsf Forschungszentrum Umwelt Einsatz mutierter Erkennungssequenzen für mehrfache aufeinander folgende Rekombinase-vermittelte Rekombinationen in einem genetischen System
ATE384129T1 (de) * 2001-11-22 2008-02-15 Univ Keio Künstliche antikörperbibliothek mit super- repertoire
DK1594955T3 (da) * 2003-02-14 2012-06-25 Biogen Idec Inc Ekspressionskassette og vektor til transient eller stabil ekspression af eksogene molekyler
CA2428972A1 (en) * 2003-05-30 2004-11-30 Wyeth Conditional knockout method for gene trapping and gene targeting using an inducible gene silencer
US7205148B2 (en) * 2003-06-11 2007-04-17 Regeneron Pharmaceuticals, Inc. Genome mutation by intron insertion into an embryonic stem cell genome
EP1662005A1 (de) * 2004-11-26 2006-05-31 FrankGen Biotechnologie AG "Gene Trap" Vektoren, die mindestens einen Enhancer enthalten und die für zufälliges und gezieltes "Gene Trapping" geeignet sind
JPWO2006075671A1 (ja) * 2005-01-13 2008-06-12 国立大学法人京都大学 所望の染色体を除去する方法およびそれを利用したテイラーメイド医療
EP1939299A1 (de) * 2006-12-29 2008-07-02 Universität des Saarlandes TRPM4-defiziente transgene Säugetiere und ihre Verwendung
WO2008112226A2 (en) * 2007-03-13 2008-09-18 Massachusetts Institute Of Technology Cre-lox based gene knockdown constructs and methods of use thereof
CA2719267A1 (en) * 2008-04-25 2009-10-29 New York Blood Center, Inc. Abi1/hssh3bp1 conditional knockout mouse
AU2010271249B2 (en) 2009-07-09 2016-01-28 Whitehead Institute For Biomedical Research Compositions and methods for mammalian genetics and uses thereof
CA2779858C (en) * 2009-10-29 2019-10-29 Regeneron Pharmaceuticals, Inc. Multifunctional alleles
EP2599859A1 (de) 2011-11-30 2013-06-05 IMBA-Institut für Molekulare Biotechnologie GmbH Haploidzellen
EP2743345A1 (de) 2012-12-13 2014-06-18 IMBA-Institut für Molekulare Biotechnologie GmbH Dreidimensionale heterogen differenzierte Gewebekultur
WO2018229276A1 (en) * 2017-06-16 2018-12-20 Universite De Strasbourg Methods to generate conditional knock-in models
EP3638773A1 (de) 2017-06-16 2020-04-22 IMBA-Institut für Molekulare Biotechnologie GmbH Blutgefäss-organoid, verfahren zur herstellung und verwendung der besagten organoide
EP3681998A1 (de) 2017-09-11 2020-07-22 IMBA-Institut für Molekulare Biotechnologie GmbH Tumororganoidmodell
CN110484549B (zh) * 2018-04-20 2023-10-03 北京大学 基因组靶向修饰方法
CN112824529B (zh) * 2019-11-21 2022-09-09 北京大学 一种条件性基因敲除或者拯救方法

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5527695A (en) * 1993-01-29 1996-06-18 Purdue Research Foundation Controlled modification of eukaryotic genomes
AU751520B2 (en) * 1998-03-27 2002-08-15 Lexicon Pharmaceuticals, Inc. Vectors for gene mutagenesis and gene discovery

Also Published As

Publication number Publication date
EP1092768A1 (de) 2001-04-18
JP2003512053A (ja) 2003-04-02
EP1222262A1 (de) 2002-07-17
CA2387737A1 (en) 2001-04-26
EP1222262B1 (de) 2007-09-05
ES2295063T3 (es) 2008-04-16
WO2001029208A1 (en) 2001-04-26
AU782960B2 (en) 2005-09-15
AU1272301A (en) 2001-04-30
DE60036297T2 (de) 2008-05-29
DE60036297D1 (de) 2007-10-18

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