ATE372381T1 - Bedingtes 'gene trapping'-konstrukt zur unterbrechung von genen - Google Patents

Bedingtes 'gene trapping'-konstrukt zur unterbrechung von genen

Info

Publication number
ATE372381T1
ATE372381T1 AT00974397T AT00974397T ATE372381T1 AT E372381 T1 ATE372381 T1 AT E372381T1 AT 00974397 T AT00974397 T AT 00974397T AT 00974397 T AT00974397 T AT 00974397T AT E372381 T1 ATE372381 T1 AT E372381T1
Authority
AT
Austria
Prior art keywords
genes
construct
gene trapping
relates
cell
Prior art date
Application number
AT00974397T
Other languages
English (en)
Inventor
Ralf Kuehn
Melchener Harald Von
Joachim Altschmied
Original Assignee
Artemis Pharmaceuticals Gmbh
Frankgen Biotechnologie Ag
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Artemis Pharmaceuticals Gmbh, Frankgen Biotechnologie Ag filed Critical Artemis Pharmaceuticals Gmbh
Application granted granted Critical
Publication of ATE372381T1 publication Critical patent/ATE372381T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2217/00—Genetically modified animals
    • A01K2217/05—Animals comprising random inserted nucleic acids (transgenic)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2800/00—Nucleic acids vectors
    • C12N2800/30—Vector systems comprising sequences for excision in presence of a recombinase, e.g. loxP or FRT
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2800/00—Nucleic acids vectors
    • C12N2800/60—Vectors containing traps for, e.g. exons, promoters
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2840/00—Vectors comprising a special translation-regulating system
    • C12N2840/20—Vectors comprising a special translation-regulating system translation of more than one cistron
    • C12N2840/203—Vectors comprising a special translation-regulating system translation of more than one cistron having an IRES
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2840/00—Vectors comprising a special translation-regulating system
    • C12N2840/44—Vectors comprising a special translation-regulating system being a specific part of the splice mechanism, e.g. donor, acceptor

Landscapes

  • Genetics & Genomics (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Mycology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
AT00974397T 1999-10-16 2000-10-16 Bedingtes 'gene trapping'-konstrukt zur unterbrechung von genen ATE372381T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP99120592A EP1092768A1 (de) 1999-10-16 1999-10-16 Konstrukt zum bedingten einfangen und Disruption von Genen
US16201699P 1999-10-27 1999-10-27

Publications (1)

Publication Number Publication Date
ATE372381T1 true ATE372381T1 (de) 2007-09-15

Family

ID=8239213

Family Applications (1)

Application Number Title Priority Date Filing Date
AT00974397T ATE372381T1 (de) 1999-10-16 2000-10-16 Bedingtes 'gene trapping'-konstrukt zur unterbrechung von genen

Country Status (8)

Country Link
EP (2) EP1092768A1 (de)
JP (1) JP2003512053A (de)
AT (1) ATE372381T1 (de)
AU (1) AU782960B2 (de)
CA (1) CA2387737A1 (de)
DE (1) DE60036297T2 (de)
ES (1) ES2295063T3 (de)
WO (1) WO2001029208A1 (de)

Families Citing this family (21)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2002219841A1 (en) 2000-11-16 2002-05-27 Cornell Research Foundation Inc. Vectors for conditional gene inactivation
EP1416791B1 (de) * 2001-08-14 2006-05-24 FrankGen Biotechnologie AG Tiermodell mit krebs, lungenemphysem und kardiomyopathie
DE10140030C1 (de) * 2001-08-16 2002-12-19 Gsf Forschungszentrum Umwelt Einsatz mutierter Erkennungssequenzen für mehrfache aufeinander folgende Rekombinase-vermittelte Rekombinationen in einem genetischen System
DE60224720D1 (en) * 2001-11-22 2008-03-06 Univ Keio Re
PL378110A1 (pl) * 2003-02-14 2006-03-06 Biogen Idec Ma Inc. Kaseta ekspresyjna i wektor do przejściowej albo stabilnej ekspresji egzogennych cząsteczek
CA2428972A1 (en) * 2003-05-30 2004-11-30 Wyeth Conditional knockout method for gene trapping and gene targeting using an inducible gene silencer
US7205148B2 (en) * 2003-06-11 2007-04-17 Regeneron Pharmaceuticals, Inc. Genome mutation by intron insertion into an embryonic stem cell genome
EP1662005A1 (de) * 2004-11-26 2006-05-31 FrankGen Biotechnologie AG "Gene Trap" Vektoren, die mindestens einen Enhancer enthalten und die für zufälliges und gezieltes "Gene Trapping" geeignet sind
CA2598022A1 (en) * 2005-01-13 2006-07-20 Kyoto University Method for removing desired chromosome and tailor-made medical treatment utilizing the same
EP1939299A1 (de) * 2006-12-29 2008-07-02 Universität des Saarlandes TRPM4-defiziente transgene Säugetiere und ihre Verwendung
US9043994B2 (en) * 2007-03-13 2015-06-02 Massachusetts Institute Of Technology Cre-lox based gene knockdown constructs and methods of use thereof
WO2009132357A1 (en) * 2008-04-25 2009-10-29 New York Blood Center, Inc. Abi1/hssh3bp1 conditional knockout mouse
JP5886195B2 (ja) * 2009-07-09 2016-03-16 ホワイトヘッド・インスティチュート・フォア・バイオメディカル・リサーチ 哺乳動物遺伝学のための組成物および方法、ならびにその使用
CN102666854B (zh) * 2009-10-29 2016-08-24 瑞泽恩制药公司 多功能等位基因
EP2599859A1 (de) 2011-11-30 2013-06-05 IMBA-Institut für Molekulare Biotechnologie GmbH Haploidzellen
EP2743345A1 (de) 2012-12-13 2014-06-18 IMBA-Institut für Molekulare Biotechnologie GmbH Dreidimensionale heterogen differenzierte Gewebekultur
US20200332278A1 (en) * 2017-06-16 2020-10-22 Universite De Strasbourg Methods to generate conditional knock-in models
JP7901964B2 (ja) 2017-06-16 2026-08-07 イーエムベーアー－インスティテュート フュール モレクラレ バイオテクノロジー ゲゼルシャフト ミット ベシュレンクテル ハフツング 血管オルガノイド、オルガノイドの製造及び使用方法
WO2019048689A1 (en) 2017-09-11 2019-03-14 Imba - Institut Für Molekulare Biotechnologie Gmbh TUMOR ORGANOID MODEL
CN110484549B (zh) * 2018-04-20 2023-10-03 北京大学 基因组靶向修饰方法
CN112824529B (zh) * 2019-11-21 2022-09-09 北京大学 一种条件性基因敲除或者拯救方法

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5527695A (en) * 1993-01-29 1996-06-18 Purdue Research Foundation Controlled modification of eukaryotic genomes
CA2323834C (en) * 1998-03-27 2009-01-27 Lexicon Genetics Incorporated Vectors for gene mutagenesis and gene discovery

Also Published As

Publication number Publication date
WO2001029208A1 (en) 2001-04-26
DE60036297T2 (de) 2008-05-29
JP2003512053A (ja) 2003-04-02
EP1092768A1 (de) 2001-04-18
CA2387737A1 (en) 2001-04-26
EP1222262B1 (de) 2007-09-05
AU1272301A (en) 2001-04-30
ES2295063T3 (es) 2008-04-16
EP1222262A1 (de) 2002-07-17
DE60036297D1 (de) 2007-10-18
AU782960B2 (en) 2005-09-15

Similar Documents

Publication Publication Date Title
DE60036297D1 (de) Bedingtes 'gene trapping'-konstrukt zur unterbrechung von genen
DE60040060D1 (de) Kontrolle des proteinspiegels in eukaryotischen organismen
ATE323180T1 (de) Katalytische nukleinsäure und deren medizinische verwendung
DK1390049T3 (da) Sammensætninger til behandling af dyresygdomme og -syndromer omfattende transferfaktor
DE602004022061D1 (de) Verwendung von modifizierten cyclosporinen zur behandlung von hcv-erkrankungen
DE69836752D1 (de) Prostaglandinagoniste und deren verwendung zur behandlung von knochenerkrankungen
ATE203159T1 (de) Verbindungen zur behandlung von allergischen und entzündlichen erkrankungen
DE59914996D1 (en) Heterocyclisch substituierte amide als calpainhemmer
DE122012000001I1 (de) Humane antikorper gegen CTLA-4 und deren verwendungen.
ATE373672T1 (de) Synthese von methoxynukleoside und enzymatische nukleisäure moleküle
DE68922044D1 (de) 2,4-Diamino-pyrimidin-3-oxid-Derivate und deren Verwendung bei der Behandlung und zur Verhütung von Haarfall.
WO2005034855A3 (en) Photodynamic inactivation of bacterial spores
ATE547101T1 (de) Verfahren zur verwendung von diketopiperazinen und zusammensetzungen, die diese enthalten
EP0938308A4 (de) Antiparasitäre mittel
DE69637579D1 (de) Methode zur Amplifizierung von Nukleinsäure und entsprechende Regenzien dafür
ATE384437T1 (de) N-thio-nicotinamid derivate und verwandte verbindungen zur verwendung als schädlingsbekämpfungsmittel
HU9502989D0 (en) Herbicidal substituted 1-phenyl or 1-pyridinyl benzotriazoles
DE69735039D1 (de) Acetylhydrolase des Plättchen aktivierenden Faktors, und entsprechendes Gen
ATE148109T1 (de) Hiv-proteaseinhibitoren und ihre verwendung zur behandlung von aids
DE69724613D1 (de) Antimikrobielle Mittel gegen eukaryotische Mikroorganismen und Verfahren zur Unterdrückung des Wachstums von eukaryotischen Mikroorganismen unter Verwendung solcher Mittel
DE59609605D1 (de) Substituierte thiazoline und ihre verwendung zur bekämpfung von tierischen schädlingen
DE69837148D1 (de) Chimäre proteine zur behandlung von diabetes
ATE352295T1 (de) Verwendung von quinazolinen zur behandlung von t- zell vermittelten krankheiten
ATE85327T1 (de) Substituierte pyridylethanolamine, verfahren zu ihrer herstellung und ihre verwendung als leistungsfoerderer bei tieren.
DE59813854D1 (de) Tgc-verfahren zur induktion einer zielgerichteten, somatischen transgenität

Legal Events

Date Code Title Description
RER Ceased as to paragraph 5 lit. 3 law introducing patent treaties