ATE373236T1 - Puffer-arrays zum nachweis von biomolekülen anhand ihres isoelektrischen punktes - Google Patents

Puffer-arrays zum nachweis von biomolekülen anhand ihres isoelektrischen punktes

Info

Publication number
ATE373236T1
ATE373236T1 AT02756507T AT02756507T ATE373236T1 AT E373236 T1 ATE373236 T1 AT E373236T1 AT 02756507 T AT02756507 T AT 02756507T AT 02756507 T AT02756507 T AT 02756507T AT E373236 T1 ATE373236 T1 AT E373236T1
Authority
AT
Austria
Prior art keywords
isoelectric point
arrays
detecting biomolecules
buffer arrays
buffer
Prior art date
Application number
AT02756507T
Other languages
English (en)
Inventor
Shmuel Bukshpan
Gleb Zilberstein
Original Assignee
Protein Forest Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Protein Forest Inc filed Critical Protein Forest Inc
Application granted granted Critical
Publication of ATE373236T1 publication Critical patent/ATE373236T1/de

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44756—Apparatus specially adapted therefor
    • G01N27/44795—Isoelectric focusing
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44704—Details; Accessories
    • G01N27/44747—Composition of gel or of carrier mixture

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Electrochemistry (AREA)
  • Pathology (AREA)
  • Dispersion Chemistry (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Transition And Organic Metals Composition Catalysts For Addition Polymerization (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
AT02756507T 2001-07-16 2002-07-16 Puffer-arrays zum nachweis von biomolekülen anhand ihres isoelektrischen punktes ATE373236T1 (de)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US30580201P 2001-07-16 2001-07-16
US31031601P 2001-08-06 2001-08-06
US34069801P 2001-10-29 2001-10-29
US37704402P 2002-04-30 2002-04-30

Publications (1)

Publication Number Publication Date
ATE373236T1 true ATE373236T1 (de) 2007-09-15

Family

ID=27501888

Family Applications (1)

Application Number Title Priority Date Filing Date
AT02756507T ATE373236T1 (de) 2001-07-16 2002-07-16 Puffer-arrays zum nachweis von biomolekülen anhand ihres isoelektrischen punktes

Country Status (12)

Country Link
US (2) US7166202B2 (de)
EP (1) EP1410029B1 (de)
JP (1) JP4108037B2 (de)
CN (1) CN1549924A (de)
AT (1) ATE373236T1 (de)
AU (1) AU2002322513B2 (de)
BR (1) BR0211213A (de)
CA (1) CA2454045A1 (de)
DE (1) DE60222427T2 (de)
DK (1) DK1410029T3 (de)
IL (1) IL159810A0 (de)
WO (1) WO2003008977A2 (de)

Families Citing this family (43)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE60222427T2 (de) * 2001-07-16 2008-06-12 Protein Forest, Inc., Waltham Puffer-arrays zum nachweis von biomolekülen anhand ihres isoelektrischen punktes
US7850835B2 (en) 2003-05-09 2010-12-14 Life Technologies Corporation Solution phase electrophoresis device, components, and methods
US7622028B2 (en) 2003-05-09 2009-11-24 Life Technologies Corporation Solution phase electrophoresis device, components, and methods
AU2004269196B2 (en) 2003-09-03 2010-03-04 Shmuel Bukshpan Methods and apparatus for rapid crystallization of biomolecules
WO2005029060A1 (en) * 2003-09-24 2005-03-31 Agilent Technologies, Inc. Electrophoretic separation of amphoteric molecules
WO2005047881A2 (en) * 2003-11-05 2005-05-26 Exact Sciences Corporation Repetitive reversed-field affinity electrophoresis and uses therefor
WO2005089910A1 (en) * 2004-03-17 2005-09-29 Ciphergen Biosystems, Inc. Multi-compartment filter and method of filtering using same
JP4644610B2 (ja) * 2006-02-17 2011-03-02 京都市 等電点電気泳動装置
US20070209939A1 (en) * 2006-03-10 2007-09-13 Protein Forest, Inc. Two-dimensional transfer device
EP2049260B1 (de) 2006-08-03 2018-09-26 Agilent Technologies, Inc. Kanalfreies transportmedium für fluidprobe
CN101568828B (zh) * 2006-11-21 2013-10-23 麦迪美特控股有限公司 用于液体的离子传感器及其制造方法
CN103499628A (zh) * 2006-11-21 2014-01-08 麦迪美特控股有限公司 用于液体的离子传感器及其制造方法
US9017536B2 (en) * 2006-12-26 2015-04-28 Sekisui Chemical Co., Ltd. Hemoglobin measurement method and electrophoresis apparatus
US20080272002A1 (en) * 2007-05-03 2008-11-06 Protein Forest, Inc. System and Method for Proteomics
GB2452239B (en) * 2007-06-01 2012-08-29 Kratos Analytical Ltd Method and apparatus useful for imaging
JP2009042004A (ja) * 2007-08-07 2009-02-26 Norio Okuyama 電気泳動用支持体
CN101868425B (zh) 2007-08-27 2013-01-02 技术研究及发展基金有限公司 通过电解控制pH梯度以及它们在等电聚焦中的用途
US20090110833A1 (en) * 2007-10-31 2009-04-30 Gala Industries, Inc. Method for abrasion-resistant non-stick surface treatments for pelletization and drying process equipment components
US9032441B2 (en) * 2007-12-21 2015-05-12 BrightTALK Limited System and method for self management of a live web event
WO2009102978A2 (en) * 2008-02-14 2009-08-20 University Of Notre Dame Du Lac Ac field induced biomolecule crystallization and hydration cage disruption
EP2146200A1 (de) * 2008-07-16 2010-01-20 Agilent Technologies, Inc. Vorrichtung und Verfahren zur isoelektrischen Fokussierung
WO2010023589A1 (en) * 2008-08-28 2010-03-04 Koninklijke Philips Electronics N.V. Method for preparing a ph gradient in an isoelectric focusing biochip
CN101556260B (zh) * 2009-05-25 2012-01-11 北京理工大学 固定化pH梯度毛细管等电聚焦测定微生物等电点的方法
US9182411B2 (en) * 2009-07-14 2015-11-10 Colen Innovations, L.L.C. Methods and apparatus for the detection and differentiation of non-sialated proteins from sialated proteins in a fluid sample
AU2010286047B2 (en) * 2009-08-18 2014-09-25 Technion Research & Development Foundation Ltd. Proton concentration topographies, methods and devices for producing the same
JP5635609B2 (ja) 2009-08-18 2014-12-03 テクニオン リサーチ アンド ディベロップメント ファウンデーションリミテッド 分子状分析物を分離する方法およびデバイス
KR101431769B1 (ko) * 2009-12-10 2014-08-20 삼성전자주식회사 당화 혈색소 측정용 원심력 기반의 미세유동 구조물, 당화 혈색소 측정용 원심력 기반 미세유동 장치 및 당화 혈색소의 측정방법
US20120190129A1 (en) * 2011-01-20 2012-07-26 Konica Minolta Holdings, Inc. Test device, reaction apparatus and reactive test method
WO2013028924A1 (en) * 2011-08-23 2013-02-28 Board Of Regents, The University Of Texas System Electrolytic buffer generator
US8917096B2 (en) 2012-11-15 2014-12-23 International Business Machines Corporation Determination of isoelectric points of biomolecules using capacitive sensors
US9250206B2 (en) 2013-04-04 2016-02-02 International Business Machines Corporation Controlled translocation of macromolecules employing a funnel nanopore structure and a gel
CN103412029B (zh) * 2013-06-26 2018-10-16 华东理工大学 基于芯片级别的平板电层析氨基酸分离装置及其使用方法
US10640531B2 (en) * 2014-05-21 2020-05-05 Unchained Labs Systems and methods for exchange of buffer solutions
DE202016008838U1 (de) 2015-11-30 2020-01-20 Intabio, Inc. Vorrichtungen zur Probencharakterisierung
EP3655779A1 (de) 2017-07-20 2020-05-27 CytomX Therapeutics, Inc. Verfahren zur qualitativen und/oder quantitativen analyse von eigenschaften von aktivierbaren antikörpern und deren verwendungen
CA3089842A1 (en) 2018-01-29 2019-08-01 Intabio, Inc. Devices, methods and kits for sample characterization
WO2019232397A1 (en) 2018-05-31 2019-12-05 Intabio, Inc. Software for microfluidic systems interfacing with mass spectrometry
CN110327994B (zh) * 2019-07-11 2020-12-08 北京理工大学 一种多维微流控电泳芯片及检测装置，检测方法
US11285484B2 (en) 2019-08-12 2022-03-29 Intabio, Llc Multichannel isoelectric focusing devices and high voltage power supplies
WO2021154854A1 (en) * 2020-01-27 2021-08-05 Bristol-Myers Squibb Company Isoelectric focusing sample matrix
CN111413184B (zh) * 2020-05-02 2025-08-01 诒福生物科技南通有限公司 染色装置以及生物组织标记设备
US12449396B2 (en) * 2020-07-28 2025-10-21 Life Technologies Corporation Electrotransfer and electrophoresis devices, systems, and methods
EP4381279A1 (de) * 2021-08-05 2024-06-12 Agilent Technologies, Inc. Systeme und verfahren im zusammenhang mit kapillararray-fensterhaltern

Family Cites Families (33)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1979000002A1 (en) 1977-06-15 1979-01-11 Nat Res Dev Improvements relating to membrane electrophoresis
US4473452A (en) 1982-11-18 1984-09-25 The Trustees Of Columbia University In The City Of New York Electrophoresis using alternating transverse electric fields
EP0287513B1 (de) 1987-04-11 1992-12-02 Ciba-Geigy Ag Isoelektrisches Fokussierverfahren sowie Einrichtung zur Durchführung dieses Verfahrens
US5114555A (en) 1988-01-05 1992-05-19 Monsanto Company Continuous isoelectric separation
US4900414A (en) * 1988-08-19 1990-02-13 Drug Delivery Systems Inc. Commercial separation system and method using electrokinetic techniques
US5160594A (en) * 1989-03-08 1992-11-03 Board Of Regents Of The University Of Texas System Apparatus and methods for isoelectric focusing of amphoteric substances incorporating ion selective membranes in electrode chambers
GB9007922D0 (en) * 1990-04-07 1990-06-06 Oxford Virology Plc A diagnostic test method
US5336387A (en) 1990-09-11 1994-08-09 Bioseparations, Inc. Electrical separator apparatus and method of counterflow gradient focusing
US6017696A (en) 1993-11-01 2000-01-25 Nanogen, Inc. Methods for electronic stringency control for molecular biological analysis and diagnostics
JP2578285B2 (ja) * 1992-02-28 1997-02-05 矢崎総業株式会社 防水コネクタ
US5376249A (en) 1992-11-25 1994-12-27 Perseptive Biosystems, Inc. Analysis utilizing isoelectric focusing
US5571410A (en) 1994-10-19 1996-11-05 Hewlett Packard Company Fully integrated miniaturized planar liquid sample handling and analysis device
US5630924A (en) 1995-04-20 1997-05-20 Perseptive Biosystems, Inc. Compositions, methods and apparatus for ultrafast electroseparation analysis
GB9509410D0 (en) 1995-05-10 1995-07-05 Imperial College Molecular imaging
IT1282607B1 (it) 1996-02-13 1998-03-31 Pier Giorgio Righetti Uso di gradienti multipli per l'analisi di mutazioni in dna genomico umano
JP2001504937A (ja) * 1996-09-28 2001-04-10 フール、ギュンター 等電式に粒子を分離するための方法及び装置
US5885430A (en) 1996-10-04 1999-03-23 Spectrumedix Corporation Capillary tube holder for an electrophoretic apparatus
US5773645A (en) 1997-05-05 1998-06-30 Bio-Rad Laboratories, Inc. Two-dimensional electrophoresis device
US5993627A (en) 1997-06-24 1999-11-30 Large Scale Biology Corporation Automated system for two-dimensional electrophoresis
US5968829A (en) * 1997-09-05 1999-10-19 Cytotherapeutics, Inc. Human CNS neural stem cells
WO1999018438A1 (en) 1997-10-02 1999-04-15 Aclara Biosciences, Inc. Capillary assays involving separation of free and bound species
US6277259B1 (en) 1998-04-24 2001-08-21 Enterprise Partners Ii High performance multidimensional proteome analyzer
US6013165A (en) 1998-05-22 2000-01-11 Lynx Therapeutics, Inc. Electrophoresis apparatus and method
US6576478B1 (en) 1998-07-14 2003-06-10 Zyomyx, Inc. Microdevices for high-throughput screening of biomolecules
US6406921B1 (en) 1998-07-14 2002-06-18 Zyomyx, Incorporated Protein arrays for high-throughput screening
EP0977030B1 (de) 1998-07-29 2001-03-21 Hewlett-Packard Company Chip zur elektroforetischen Trennung von Molekülen und Verfahren zur Verwendung desselben
SE9803224D0 (sv) 1998-09-23 1998-09-23 Amersham Pharm Biotech Ab Method for separation of macromolecules
US6240790B1 (en) 1998-11-09 2001-06-05 Agilent Technologies, Inc. Device for high throughout sample processing, analysis and collection, and methods of use thereof
US6156182A (en) 1998-11-19 2000-12-05 Bio-Rad Laboratories, Inc. Encapsulated IPG Strips
EP1044716A1 (de) * 1999-03-13 2000-10-18 Michael Dr. Cahill Mikropräparative isoelektrischen Fokussierung
US6638408B1 (en) * 2000-04-03 2003-10-28 The Wistar Institute Method and device for separation of charged molecules by solution isoelectric focusing
US6404905B1 (en) * 2000-08-31 2002-06-11 Large Scale Proteomics Corp. Method and apparatus for impressing a master pattern to a gel image
DE60222427T2 (de) * 2001-07-16 2008-06-12 Protein Forest, Inc., Waltham Puffer-arrays zum nachweis von biomolekülen anhand ihres isoelektrischen punktes

Also Published As

Publication number Publication date
AU2002322513B2 (en) 2008-02-28
US20030102215A1 (en) 2003-06-05
BR0211213A (pt) 2006-05-23
US20070138015A1 (en) 2007-06-21
US7166202B2 (en) 2007-01-23
JP2005526949A (ja) 2005-09-08
IL159810A0 (en) 2004-06-20
DE60222427D1 (de) 2007-10-25
JP4108037B2 (ja) 2008-06-25
EP1410029B1 (de) 2007-09-12
CN1549924A (zh) 2004-11-24
EP1410029A2 (de) 2004-04-21
WO2003008977A2 (en) 2003-01-30
WO2003008977A3 (en) 2003-06-19
DK1410029T3 (da) 2008-01-07
US7914656B2 (en) 2011-03-29
CA2454045A1 (en) 2003-01-30
DE60222427T2 (de) 2008-06-12

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