ATE373673T1 - Verbessertes verfahren zur behandlung durch bisulfit - Google Patents
Verbessertes verfahren zur behandlung durch bisulfitInfo
- Publication number
- ATE373673T1 ATE373673T1 AT04705800T AT04705800T ATE373673T1 AT E373673 T1 ATE373673 T1 AT E373673T1 AT 04705800 T AT04705800 T AT 04705800T AT 04705800 T AT04705800 T AT 04705800T AT E373673 T1 ATE373673 T1 AT E373673T1
- Authority
- AT
- Austria
- Prior art keywords
- solution
- nucleic acid
- bisulfite
- improved method
- bisulfite treatment
- Prior art date
Links
- LSNNMFCWUKXFEE-UHFFFAOYSA-M Bisulfite Chemical compound OS([O-])=O LSNNMFCWUKXFEE-UHFFFAOYSA-M 0.000 title abstract 4
- 238000000034 method Methods 0.000 title abstract 2
- 150000007523 nucleic acids Chemical class 0.000 abstract 6
- 102000039446 nucleic acids Human genes 0.000 abstract 6
- 108020004707 nucleic acids Proteins 0.000 abstract 6
- OPTASPLRGRRNAP-UHFFFAOYSA-N cytosine Chemical compound NC=1C=CNC(=O)N=1 OPTASPLRGRRNAP-UHFFFAOYSA-N 0.000 abstract 2
- 238000006243 chemical reaction Methods 0.000 abstract 1
- 229940104302 cytosine Drugs 0.000 abstract 1
- 230000011987 methylation Effects 0.000 abstract 1
- 238000007069 methylation reaction Methods 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
- C07H21/04—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids with deoxyribosyl as saccharide radical
-
- C—CHEMISTRY; METALLURGY
- C01—INORGANIC CHEMISTRY
- C01D—COMPOUNDS OF ALKALI METALS, i.e. LITHIUM, SODIUM, POTASSIUM, RUBIDIUM, CAESIUM, OR FRANCIUM
- C01D5/00—Sulfates or sulfites of sodium, potassium or alkali metals in general
- C01D5/14—Preparation of sulfites
- C01D5/145—Pyrosulfites or metabisulfites
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Biophysics (AREA)
- Materials Engineering (AREA)
- Microbiology (AREA)
- Analytical Chemistry (AREA)
- Physics & Mathematics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Immunology (AREA)
- Inorganic Chemistry (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Saccharide Compounds (AREA)
- Compounds Of Iron (AREA)
- Silver Salt Photography Or Processing Solution Therefor (AREA)
- Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP03001854A EP1443052A1 (de) | 2003-01-29 | 2003-01-29 | Verbessertes Verfahren zur Nukleinsäure Behandlung mit Bisulfit |
| EP03010020 | 2003-05-02 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ATE373673T1 true ATE373673T1 (de) | 2007-10-15 |
Family
ID=32826770
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AT04705800T ATE373673T1 (de) | 2003-01-29 | 2004-01-28 | Verbessertes verfahren zur behandlung durch bisulfit |
Country Status (7)
| Country | Link |
|---|---|
| US (1) | US7413855B2 (de) |
| EP (1) | EP1590362B8 (de) |
| JP (2) | JP2006516391A (de) |
| AT (1) | ATE373673T1 (de) |
| DE (1) | DE602004009038T3 (de) |
| ES (1) | ES2294462T5 (de) |
| WO (1) | WO2004067545A1 (de) |
Families Citing this family (31)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| ATE373673T1 (de) † | 2003-01-29 | 2007-10-15 | Hoffmann La Roche | Verbessertes verfahren zur behandlung durch bisulfit |
| US7371526B2 (en) | 2003-08-29 | 2008-05-13 | Applera Corporation | Method and materials for bisulfite conversion of cytosine to uracil |
| US7262013B2 (en) | 2003-08-29 | 2007-08-28 | Applera Corporation | Bisulfite method |
| US7368239B2 (en) | 2003-08-29 | 2008-05-06 | Applera Corporation | Method and materials for polyamine catalyzed bisulfite conversion of cytosine to uracil |
| US7534873B2 (en) | 2003-08-29 | 2009-05-19 | Applied Biosystems, Llc | Method and materials for quaternary amine catalyzed bisulfite conversion of cytosine to uracil |
| CN1863927B (zh) | 2003-09-04 | 2010-08-11 | 人类遗传标记控股有限公司 | 核酸检测分析 |
| JPWO2005100240A1 (ja) * | 2004-04-08 | 2008-03-06 | 東洋紡績株式会社 | Ｄｎａの脱アミノ化のための組成物及びメチル化ｄｎａの検出方法 |
| EP1632578A1 (de) | 2004-09-03 | 2006-03-08 | Roche Diagnostics GmbH | Methode zur Dekontamination der DNA |
| US7658288B2 (en) | 2004-11-08 | 2010-02-09 | Applied Biosystems, Llc | Bisulfite conversion reagent |
| ES2399054T3 (es) | 2004-12-03 | 2013-03-25 | Human Genetic Signatures Pty Ltd | Métodos para simplificar ácidos nucleicos microbianos mediante modificación química de citosinas |
| US8129107B2 (en) | 2005-04-01 | 2012-03-06 | Epigenomics Ag | Bisulfite conversion of DNA |
| AU2006251866B2 (en) * | 2005-05-26 | 2007-11-29 | Human Genetic Signatures Pty Ltd | Isothermal strand displacement amplification using primers containing a non-regular base |
| US20060286577A1 (en) * | 2005-06-17 | 2006-12-21 | Xiyu Jia | Methods for detection of methylated DNA |
| ATE531820T1 (de) * | 2005-09-14 | 2011-11-15 | Human Genetic Signatures Pty | Gesundheitszustandstest |
| CA2631829C (en) | 2005-12-14 | 2012-01-24 | F. Hoffmann-La Roche Ag | New method for bisulfite treatment |
| WO2007123553A1 (en) * | 2006-04-24 | 2007-11-01 | Xiyu Jia | Methods for detection of methylated dna |
| US20080050738A1 (en) * | 2006-05-31 | 2008-02-28 | Human Genetic Signatures Pty Ltd. | Detection of target nucleic acid |
| WO2008109945A1 (en) * | 2007-03-12 | 2008-09-18 | Human Genetic Signatures Pty Ltd | Analysis of ribonucleic acid |
| JP2010521142A (ja) * | 2007-03-16 | 2010-06-24 | ヒューマン ジェネティック シグネチャーズ ピーティーワイ リミテッド | 遺伝子発現アッセイ |
| AU2008329549B2 (en) * | 2007-11-27 | 2013-08-22 | Human Genetic Signatures Pty Ltd | Enzymes for amplification and copying bisulphite modified nucleic acids |
| EP2215035A4 (de) * | 2007-12-05 | 2011-12-14 | Human Genetic Signatures Pty | Bisulfitbehandlung von rna |
| CA2709632A1 (en) * | 2007-12-20 | 2009-07-02 | Human Genetic Signatures Pty Ltd | Elimination of contaminants associated with nucleic acid amplification |
| US9637779B2 (en) * | 2008-12-04 | 2017-05-02 | The Johns Hopkins University | Antisense transcriptomes of cells |
| FR2940805B1 (fr) | 2009-01-05 | 2015-10-16 | Biomerieux Sa | Procede d'amplification et/ou de detection d'acides nucleiques, kits et utilisations de ce procede |
| DK2420578T3 (en) * | 2009-04-17 | 2017-10-02 | Nat Defense Medical Center | A method of screening for cancer |
| DK2753710T3 (en) | 2011-09-07 | 2017-02-06 | Human Genetic Signatures Pty Ltd | MOLECULAR DETECTION ASSAY |
| CA3111723A1 (en) | 2012-01-30 | 2013-08-08 | Exact Sciences Development Company, Llc | Sulfonated small dna compositions and methods for sulfonating and desulfonating small dnas |
| EP2935583B1 (de) * | 2012-12-20 | 2018-09-12 | General Electric Company | Formulierungen für nukleinsäurestabilisierung auf festen substraten |
| GB2587939B (en) | 2018-04-02 | 2023-06-14 | Grail Llc | Methylation markers and targeted methylation probe panels |
| CA3111887A1 (en) | 2018-09-27 | 2020-04-02 | Grail, Inc. | Methylation markers and targeted methylation probe panel |
| CN115246823B (zh) * | 2022-09-01 | 2024-02-20 | 渤海大学 | 一种检测亚硫酸氢盐和指示鱼肉新鲜度的双功能近红外发射荧光探针及其合成方法和应用 |
Family Cites Families (28)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4335148A (en) † | 1980-06-04 | 1982-06-15 | Pennwalt Corporation | Methods of preventing grain sprouting after harvest through the application of sulfur dioxide, nitrogen and ammonia gases |
| US4683202A (en) | 1985-03-28 | 1987-07-28 | Cetus Corporation | Process for amplifying nucleic acid sequences |
| DK171161B1 (da) | 1985-03-28 | 1996-07-08 | Hoffmann La Roche | Fremgangsmåde til påvisning af forekomst eller fravær af mindst én specifik nukleinsyresekvens i en prøve eller til skelnen mellem to forskellige nukleinsyresekvenser i denne prøve |
| US4844880A (en) * | 1985-12-27 | 1989-07-04 | Hmc Patents Holding Co., Inc. | Process for the manufacture of sodium metabisulfite |
| IE61148B1 (en) | 1988-03-10 | 1994-10-05 | Ici Plc | Method of detecting nucleotide sequences |
| US5130238A (en) | 1988-06-24 | 1992-07-14 | Cangene Corporation | Enhanced nucleic acid amplification process |
| DE68926504T2 (de) | 1988-07-20 | 1996-09-12 | David Segev | Verfahren zur amplifizierung und zum nachweis von nukleinsäuresequenzen |
| US5639611A (en) | 1988-12-12 | 1997-06-17 | City Of Hope | Allele specific polymerase chain reaction |
| US5234809A (en) | 1989-03-23 | 1993-08-10 | Akzo N.V. | Process for isolating nucleic acid |
| NL8900725A (nl) | 1989-03-23 | 1990-10-16 | Az Univ Amsterdam | Werkwijze en combinatie van middelen voor het isoleren van nucleinezuur. |
| US5137806A (en) | 1989-12-11 | 1992-08-11 | Board Of Regents, The University Of Texas System | Methods and compositions for the detection of sequences in selected DNA molecules |
| ATE137269T1 (de) | 1990-01-26 | 1996-05-15 | Abbott Lab | Verbessertes verfahren zur amplifikation von nuklein säurezielsequenz, einsetzbar für die polymerase und ligasekettenreaktion |
| US5210015A (en) | 1990-08-06 | 1993-05-11 | Hoffman-La Roche Inc. | Homogeneous assay system using the nuclease activity of a nucleic acid polymerase |
| IL100040A (en) | 1990-11-13 | 1995-12-31 | Siska Diagnostics Inc | Nucleic acid amplification by two enzyme self-sustained sequence replication |
| US5552277A (en) | 1994-07-19 | 1996-09-03 | The Johns Hopkins University School Of Medicine | Genetic diagnosis of prostate cancer |
| DE19520398B4 (de) | 1995-06-08 | 2009-04-16 | Roche Diagnostics Gmbh | Magnetisches Pigment |
| US5786146A (en) | 1996-06-03 | 1998-07-28 | The Johns Hopkins University School Of Medicine | Method of detection of methylated nucleic acid using agents which modify unmethylated cytosine and distinguishing modified methylated and non-methylated nucleic acids |
| US6174670B1 (en) | 1996-06-04 | 2001-01-16 | University Of Utah Research Foundation | Monitoring amplification of DNA during PCR |
| DE19743518A1 (de) | 1997-10-01 | 1999-04-15 | Roche Diagnostics Gmbh | Automatisierbare universell anwendbare Probenvorbereitungsmethode |
| US6355792B1 (en) | 1998-02-04 | 2002-03-12 | Merck Patent Gesellschaft | Method for isolating and purifying nucleic acids |
| ATE248911T1 (de) | 1998-11-30 | 2003-09-15 | Roche Diagnostics Gmbh | Magnetische partikel zur reinigung von nukleinsäuren |
| SE9804469L (sv) | 1998-12-21 | 2000-06-22 | Volvo Ab | Anordning vid ett motorfordon |
| US6331393B1 (en) | 1999-05-14 | 2001-12-18 | University Of Southern California | Process for high-throughput DNA methylation analysis |
| JP2003514383A (ja) | 1999-11-17 | 2003-04-15 | ロシュ ダイアグノスティクス ゲゼルシャフト ミット ベシュレンクテル ハフツング | 磁性ガラス粒子、それらの製造方法、及びそれらの使用 |
| DE10029915B4 (de) | 2000-06-19 | 2005-06-16 | Epigenomics Ag | Verfahren zum Nachweis von Cytosin-Methylierungen |
| DE10050942B4 (de) | 2000-10-10 | 2005-11-17 | Epigenomics Ag | Verfahren zum Nachweis von Cytosin-Methylierungen |
| EP1394172A1 (de) | 2002-08-29 | 2004-03-03 | Boehringer Mannheim Gmbh | Verbessertes Verfahren Zur Bisulfit-Behandlung |
| ATE373673T1 (de) † | 2003-01-29 | 2007-10-15 | Hoffmann La Roche | Verbessertes verfahren zur behandlung durch bisulfit |
-
2004
- 2004-01-28 AT AT04705800T patent/ATE373673T1/de active
- 2004-01-28 JP JP2005518651A patent/JP2006516391A/ja active Pending
- 2004-01-28 WO PCT/EP2004/000729 patent/WO2004067545A1/en not_active Ceased
- 2004-01-28 US US10/540,406 patent/US7413855B2/en not_active Expired - Fee Related
- 2004-01-28 EP EP04705800.3A patent/EP1590362B8/de not_active Expired - Lifetime
- 2004-01-28 DE DE602004009038.7T patent/DE602004009038T3/de not_active Expired - Lifetime
- 2004-01-28 ES ES04705800.3T patent/ES2294462T5/es not_active Expired - Lifetime
-
2009
- 2009-06-25 JP JP2009150412A patent/JP2009261405A/ja not_active Withdrawn
Also Published As
| Publication number | Publication date |
|---|---|
| EP1590362B1 (de) | 2007-09-19 |
| WO2004067545A1 (en) | 2004-08-12 |
| EP1590362B2 (de) | 2015-01-14 |
| DE602004009038T2 (de) | 2008-06-19 |
| JP2009261405A (ja) | 2009-11-12 |
| ES2294462T5 (es) | 2015-05-06 |
| EP1590362A1 (de) | 2005-11-02 |
| EP1590362B8 (de) | 2015-06-03 |
| DE602004009038D1 (de) | 2007-10-31 |
| US20060058518A1 (en) | 2006-03-16 |
| ES2294462T3 (es) | 2008-04-01 |
| DE602004009038T3 (de) | 2015-06-25 |
| JP2006516391A (ja) | 2006-07-06 |
| US7413855B2 (en) | 2008-08-19 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| UEP | Publication of translation of european patent specification |
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