ATE373673T1 - Verbessertes verfahren zur behandlung durch bisulfit - Google Patents

Verbessertes verfahren zur behandlung durch bisulfit

Info

Publication number
ATE373673T1
ATE373673T1 AT04705800T AT04705800T ATE373673T1 AT E373673 T1 ATE373673 T1 AT E373673T1 AT 04705800 T AT04705800 T AT 04705800T AT 04705800 T AT04705800 T AT 04705800T AT E373673 T1 ATE373673 T1 AT E373673T1
Authority
AT
Austria
Prior art keywords
solution
nucleic acid
bisulfite
improved method
bisulfite treatment
Prior art date
Application number
AT04705800T
Other languages
English (en)
Inventor
Frank Bergmann
Christine Market-Hahn
Joerg Kleiber
Dirk Block
Original Assignee
Hoffmann La Roche
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=32826770&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=ATE373673(T1) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Priority claimed from EP03001854A external-priority patent/EP1443052A1/de
Application filed by Hoffmann La Roche filed Critical Hoffmann La Roche
Application granted granted Critical
Publication of ATE373673T1 publication Critical patent/ATE373673T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C07H21/04—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids with deoxyribosyl as saccharide radical
    • C—CHEMISTRY; METALLURGY
    • C01—INORGANIC CHEMISTRY
    • C01D—COMPOUNDS OF ALKALI METALS, i.e. LITHIUM, SODIUM, POTASSIUM, RUBIDIUM, CAESIUM, OR FRANCIUM
    • C01D5/00—Sulfates or sulfites of sodium, potassium or alkali metals in general
    • C01D5/14—Preparation of sulfites
    • C01D5/145—Pyrosulfites or metabisulfites
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Materials Engineering (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Inorganic Chemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Compounds Of Iron (AREA)
  • Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
  • Silver Salt Photography Or Processing Solution Therefor (AREA)
AT04705800T 2003-01-29 2004-01-28 Verbessertes verfahren zur behandlung durch bisulfit ATE373673T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP03001854A EP1443052A1 (de) 2003-01-29 2003-01-29 Verbessertes Verfahren zur Nukleinsäure Behandlung mit Bisulfit
EP03010020 2003-05-02

Publications (1)

Publication Number Publication Date
ATE373673T1 true ATE373673T1 (de) 2007-10-15

Family

ID=32826770

Family Applications (1)

Application Number Title Priority Date Filing Date
AT04705800T ATE373673T1 (de) 2003-01-29 2004-01-28 Verbessertes verfahren zur behandlung durch bisulfit

Country Status (7)

Country Link
US (1) US7413855B2 (de)
EP (1) EP1590362B8 (de)
JP (2) JP2006516391A (de)
AT (1) ATE373673T1 (de)
DE (1) DE602004009038T3 (de)
ES (1) ES2294462T5 (de)
WO (1) WO2004067545A1 (de)

Families Citing this family (31)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ATE373673T1 (de) † 2003-01-29 2007-10-15 Hoffmann La Roche Verbessertes verfahren zur behandlung durch bisulfit
US7371526B2 (en) 2003-08-29 2008-05-13 Applera Corporation Method and materials for bisulfite conversion of cytosine to uracil
US7534873B2 (en) 2003-08-29 2009-05-19 Applied Biosystems, Llc Method and materials for quaternary amine catalyzed bisulfite conversion of cytosine to uracil
US7368239B2 (en) 2003-08-29 2008-05-06 Applera Corporation Method and materials for polyamine catalyzed bisulfite conversion of cytosine to uracil
US7262013B2 (en) 2003-08-29 2007-08-28 Applera Corporation Bisulfite method
EP1668148B1 (de) 2003-09-04 2008-12-31 Human Genetic Signatures PTY Ltd. Nukleinsäurenachweistest
WO2005100240A1 (ja) * 2004-04-08 2005-10-27 Toyo Boseki Kabushiki Kaisha Dnaの脱アミノ化のための組成物及びメチル化dnaの検出方法
EP1632578A1 (de) 2004-09-03 2006-03-08 Roche Diagnostics GmbH Methode zur Dekontamination der DNA
US7658288B2 (en) 2004-11-08 2010-02-09 Applied Biosystems, Llc Bisulfite conversion reagent
NZ555620A (en) 2004-12-03 2008-08-29 Human Genetic Signatures Pty Methods for simplifying microbial nucleic acids by chemical modification of cytosines
WO2006103111A2 (en) * 2005-04-01 2006-10-05 Epigenomics Ag Improved bisulfite conversion of dna
EP1883707B1 (de) * 2005-05-26 2014-04-09 Human Genetic Signatures PTY Ltd Isothermische strangverdrängungsamplifikation unter verwendung von eine nichtreguläre base enthaltenden primern
US20060286577A1 (en) * 2005-06-17 2006-12-21 Xiyu Jia Methods for detection of methylated DNA
CN101292046B (zh) * 2005-09-14 2013-03-13 人类遗传标记控股有限公司 健康状态的测定法
CN101331148B (zh) 2005-12-14 2012-06-27 霍夫曼-拉罗奇有限公司 用于亚硫酸氢盐处理的新方法
WO2007123553A1 (en) * 2006-04-24 2007-11-01 Xiyu Jia Methods for detection of methylated dna
US20080050738A1 (en) * 2006-05-31 2008-02-28 Human Genetic Signatures Pty Ltd. Detection of target nucleic acid
WO2008109945A1 (en) * 2007-03-12 2008-09-18 Human Genetic Signatures Pty Ltd Analysis of ribonucleic acid
US20100092972A1 (en) * 2007-03-16 2010-04-15 Human Genetic Signatures Pty Ltd. Assay for gene expression
AU2008329549B2 (en) * 2007-11-27 2013-08-22 Human Genetic Signatures Pty Ltd Enzymes for amplification and copying bisulphite modified nucleic acids
JP2011505153A (ja) * 2007-12-05 2011-02-24 ヒューマン ジェネティック シグネチャーズ ピーティーワイ リミテッド Ｒｎａのバイサルファイト処理
JP2011505845A (ja) * 2007-12-20 2011-03-03 ヒューマン ジェネティック シグネチャーズ ピーティーワイ リミテッド 核酸増幅に伴う汚染物質の排除
US9637779B2 (en) * 2008-12-04 2017-05-02 The Johns Hopkins University Antisense transcriptomes of cells
FR2940805B1 (fr) 2009-01-05 2015-10-16 Biomerieux Sa Procede d'amplification et/ou de detection d'acides nucleiques, kits et utilisations de ce procede
KR101636596B1 (ko) * 2009-04-17 2016-07-11 내셔널 디펜스 메디컬 센터 검사 표지로서 표적 유전자의 메틸화율을 검출하는 방법
KR101912488B1 (ko) 2011-09-07 2018-10-26 휴먼 제네틱 시그너처스 피티와이 엘티디 분자 검출 분석법
ES2675584T3 (es) 2012-01-30 2018-07-11 Exact Sciences Development Company, Llc Modificación de ADN en perlas magnéticas
JP6544860B2 (ja) * 2012-12-20 2019-07-17 ゼネラル・エレクトリック・カンパニイ 固体基材上での核酸安定化のための配合物
AU2019249422B2 (en) 2018-04-02 2025-09-25 Grail, Inc. Methylation markers and targeted methylation probe panels
IL281741B2 (en) 2018-09-27 2026-04-01 Grail Inc Methylation markers and a targeted methylation detection panel
CN115246823B (zh) * 2022-09-01 2024-02-20 渤海大学 一种检测亚硫酸氢盐和指示鱼肉新鲜度的双功能近红外发射荧光探针及其合成方法和应用

Family Cites Families (28)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4335148A (en) † 1980-06-04 1982-06-15 Pennwalt Corporation Methods of preventing grain sprouting after harvest through the application of sulfur dioxide, nitrogen and ammonia gases
ES8706823A1 (es) 1985-03-28 1987-06-16 Cetus Corp Un procedimiento para detectar la presencia o ausencia de al menos una secuencia especifica de acidos nucleicos en una muestra
US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US4844880A (en) * 1985-12-27 1989-07-04 Hmc Patents Holding Co., Inc. Process for the manufacture of sodium metabisulfite
IE61148B1 (en) 1988-03-10 1994-10-05 Ici Plc Method of detecting nucleotide sequences
US5130238A (en) 1988-06-24 1992-07-14 Cangene Corporation Enhanced nucleic acid amplification process
WO1990001069A1 (en) 1988-07-20 1990-02-08 Segev Diagnostics, Inc. Process for amplifying and detecting nucleic acid sequences
US5639611A (en) 1988-12-12 1997-06-17 City Of Hope Allele specific polymerase chain reaction
NL8900725A (nl) 1989-03-23 1990-10-16 Az Univ Amsterdam Werkwijze en combinatie van middelen voor het isoleren van nucleinezuur.
US5234809A (en) 1989-03-23 1993-08-10 Akzo N.V. Process for isolating nucleic acid
US5137806A (en) 1989-12-11 1992-08-11 Board Of Regents, The University Of Texas System Methods and compositions for the detection of sequences in selected DNA molecules
DE69118930T2 (de) 1990-01-26 1997-01-09 Abbott Lab Verbessertes Verfahren zur Amplifikation von Nuklein säurezielsequenz, einsetzbar für die Polymerase und Ligasekettenreaktion
US5210015A (en) 1990-08-06 1993-05-11 Hoffman-La Roche Inc. Homogeneous assay system using the nuclease activity of a nucleic acid polymerase
IE913930A1 (en) 1990-11-13 1992-06-17 Siska Diagnostics Nucleic acid amplification by two-enzyme, self-sustained¹sequence replication
US5552277A (en) 1994-07-19 1996-09-03 The Johns Hopkins University School Of Medicine Genetic diagnosis of prostate cancer
WO2000032762A1 (de) 1998-11-30 2000-06-08 Roche Diagnostics Gmbh Magnetische partikel zur reinigung von nukleinsäuren
DE19520398B4 (de) 1995-06-08 2009-04-16 Roche Diagnostics Gmbh Magnetisches Pigment
US5786146A (en) 1996-06-03 1998-07-28 The Johns Hopkins University School Of Medicine Method of detection of methylated nucleic acid using agents which modify unmethylated cytosine and distinguishing modified methylated and non-methylated nucleic acids
ATE428801T1 (de) 1996-06-04 2009-05-15 Univ Utah Res Found Überwachung der hybridisierung während pcr
DE19743518A1 (de) 1997-10-01 1999-04-15 Roche Diagnostics Gmbh Automatisierbare universell anwendbare Probenvorbereitungsmethode
DE59912604D1 (de) 1998-02-04 2005-11-03 Merck Patent Gmbh Verfahren zur isolierung und aufreinigung von nucleinsäuren
SE9804469L (sv) 1998-12-21 2000-06-22 Volvo Ab Anordning vid ett motorfordon
US6331393B1 (en) 1999-05-14 2001-12-18 University Of Southern California Process for high-throughput DNA methylation analysis
ATE315826T1 (de) 1999-11-17 2006-02-15 Roche Diagnostics Gmbh Magnetische glasteilchen, herstellungsverfahren und benutzung
DE10029915B4 (de) 2000-06-19 2005-06-16 Epigenomics Ag Verfahren zum Nachweis von Cytosin-Methylierungen
DE10050942B4 (de) 2000-10-10 2005-11-17 Epigenomics Ag Verfahren zum Nachweis von Cytosin-Methylierungen
EP1394172A1 (de) 2002-08-29 2004-03-03 Boehringer Mannheim Gmbh Verbessertes Verfahren Zur Bisulfit-Behandlung
ATE373673T1 (de) † 2003-01-29 2007-10-15 Hoffmann La Roche Verbessertes verfahren zur behandlung durch bisulfit

Also Published As

Publication number Publication date
US7413855B2 (en) 2008-08-19
EP1590362B1 (de) 2007-09-19
DE602004009038D1 (de) 2007-10-31
EP1590362B2 (de) 2015-01-14
EP1590362B8 (de) 2015-06-03
DE602004009038T2 (de) 2008-06-19
JP2006516391A (ja) 2006-07-06
JP2009261405A (ja) 2009-11-12
US20060058518A1 (en) 2006-03-16
ES2294462T3 (es) 2008-04-01
WO2004067545A1 (en) 2004-08-12
EP1590362A1 (de) 2005-11-02
ES2294462T5 (es) 2015-05-06
DE602004009038T3 (de) 2015-06-25

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