ATE374820T1 - Mutantenluciferase - Google Patents

Mutantenluciferase

Info

Publication number: ATE374820T1
Authority: AT; Austria
Prior art keywords: luciferase; amino acid; compared; wild; type
Prior art date: 1999-10-26

Application number

AT00971589T

Other languages

English (en)

Inventor

David Squirrell

Melenie Murphy

Rachel Price

Peter White

Tara Willey

Original Assignee

Secr Defence

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

1999-10-26

Filing date

2000-10-26

Publication date

2007-10-15

1999-10-26 Priority claimed from GBGB9925161.3A external-priority patent/GB9925161D0/en

2000-10-26 Application filed by Secr Defence filed Critical Secr Defence

2007-10-15 Application granted granted Critical

2007-10-15 Publication of ATE374820T1 publication Critical patent/ATE374820T1/de

Links

108060001084 Luciferase Proteins 0.000 title abstract 9
239000005089 Luciferase Substances 0.000 title abstract 7
150000001413 amino acids Chemical class 0.000 abstract 3
230000002378 acidificating effect Effects 0.000 abstract 2
RGJOEKWQDUBAIZ-IBOSZNHHSA-N CoASH Chemical compound O[C@@H]1[C@H](OP(O)(O)=O)[C@@H](COP(O)(=O)OP(O)(=O)OCC(C)(C)[C@@H](O)C(=O)NCCC(=O)NCCS)O[C@H]1N1C2=NC=NC(N)=C2N=C1 RGJOEKWQDUBAIZ-IBOSZNHHSA-N 0.000 abstract 1
108090000790 Enzymes Proteins 0.000 abstract 1
102000004190 Enzymes Human genes 0.000 abstract 1
OUYCCCASQSFEME-QMMMGPOBSA-N L-tyrosine Chemical compound OC(=O)[C@@H](N)CC1=CC=C(O)C=C1 OUYCCCASQSFEME-QMMMGPOBSA-N 0.000 abstract 1
241000254064 Photinus pyralis Species 0.000 abstract 1
101000622060 Photinus pyralis Luciferin 4-monooxygenase Proteins 0.000 abstract 1
102000007056 Recombinant Fusion Proteins Human genes 0.000 abstract 1
108010008281 Recombinant Fusion Proteins Proteins 0.000 abstract 1
125000000539 amino acid group Chemical group 0.000 abstract 1
238000003556 assay Methods 0.000 abstract 1
RGJOEKWQDUBAIZ-UHFFFAOYSA-N coenzime A Natural products OC1C(OP(O)(O)=O)C(COP(O)(=O)OP(O)(=O)OCC(C)(C)C(O)C(=O)NCCC(=O)NCCS)OC1N1C2=NC=NC(N)=C2N=C1 RGJOEKWQDUBAIZ-UHFFFAOYSA-N 0.000 abstract 1
239000005516 coenzyme A Substances 0.000 abstract 1
229940093530 coenzyme a Drugs 0.000 abstract 1
KDTSHFARGAKYJN-UHFFFAOYSA-N dephosphocoenzyme A Natural products OC1C(O)C(COP(O)(=O)OP(O)(=O)OCC(C)(C)C(O)C(=O)NCCC(=O)NCCS)OC1N1C2=NC=NC(N)=C2N=C1 KDTSHFARGAKYJN-UHFFFAOYSA-N 0.000 abstract 1
230000000694 effects Effects 0.000 abstract 1
OUYCCCASQSFEME-UHFFFAOYSA-N tyrosine Natural products OC(=O)C(N)CC1=CC=C(O)C=C1 OUYCCCASQSFEME-UHFFFAOYSA-N 0.000 abstract 1

Classifications

- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/0004—Oxidoreductases (1.)
- C12N9/0069—Oxidoreductases (1.) acting on single donors with incorporation of molecular oxygen, i.e. oxygenases (1.13)
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y113/00—Oxidoreductases acting on single donors with incorporation of molecular oxygen (oxygenases) (1.13)
- C12Y113/12—Oxidoreductases acting on single donors with incorporation of molecular oxygen (oxygenases) (1.13) with incorporation of one atom of oxygen (internal monooxygenases or internal mixed function oxidases)(1.13.12)
- C12Y113/12007—Photinus-luciferin 4-monooxygenase (ATP-hydrolysing) (1.13.12.7), i.e. firefly-luciferase
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
- G01N2333/90—Enzymes; Proenzymes
- G01N2333/902—Oxidoreductases (1.)
- G01N2333/90241—Oxidoreductases (1.) acting on single donors with incorporation of molecular oxygen, i.e. oxygenases (1.13)

Landscapes

Chemical & Material Sciences (AREA)
Life Sciences & Earth Sciences (AREA)
Organic Chemistry (AREA)
Health & Medical Sciences (AREA)
Genetics & Genomics (AREA)
Engineering & Computer Science (AREA)
Bioinformatics & Cheminformatics (AREA)
Zoology (AREA)
Wood Science & Technology (AREA)
General Health & Medical Sciences (AREA)
Biochemistry (AREA)
General Engineering & Computer Science (AREA)
Medicinal Chemistry (AREA)
Biomedical Technology (AREA)
Biotechnology (AREA)
Molecular Biology (AREA)
Microbiology (AREA)
Enzymes And Modification Thereof (AREA)
Micro-Organisms Or Cultivation Processes Thereof (AREA)
Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
Medicines Containing Material From Animals Or Micro-Organisms (AREA)

AT00971589T 1999-10-26 2000-10-26 Mutantenluciferase ATE374820T1 (de)

Applications Claiming Priority (2)

Application Number	Priority Date	Filing Date	Title
GBGB9925161.3A GB9925161D0 (en)	1999-10-26	1999-10-26	Novel enzyme
GBGB0016744.5A GB0016744D0 (en)	1999-10-26	2000-07-10	Novel enzyme

Publications (1)

Publication Number	Publication Date
ATE374820T1 true ATE374820T1 (de)	2007-10-15

Family

ID=26244619

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
AT00971589T ATE374820T1 (de)	1999-10-26	2000-10-26	Mutantenluciferase

Country Status (12)

Country	Link
EP (1)	EP1224294B1 (de)
JP (1)	JP5154724B2 (de)
CN (1)	CN100516223C (de)
AT (1)	ATE374820T1 (de)
AU (1)	AU772485C (de)
CA (1)	CA2387691A1 (de)
DE (1)	DE60036633T2 (de)
DK (1)	DK1224294T3 (de)
ES (1)	ES2292479T3 (de)
NZ (1)	NZ518413A (de)
RU (1)	RU2251571C2 (de)
WO (1)	WO2001031028A2 (de)

Families Citing this family (30)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
US6602677B1 (en)	1997-09-19	2003-08-05	Promega Corporation	Thermostable luciferases and methods of production
GB9823468D0 (en)	1998-10-28	1998-12-23	Secr Defence	Novel enzyme
GB9925161D0 (en)	1999-10-26	1999-12-22	Secr Defence	Novel enzyme
GB0111275D0 (en)	2001-05-09	2001-06-27	Secr Defence	Analytical method and kit
GB2400659B8 (en)	2003-04-17	2007-07-09	Cambrex Bio Science Nottingham	Assay methods and materials
EP1687399A4 (de) *	2003-10-03	2007-09-05	Univ Michigan Tech	Modifizierte luciferase
GB0517005D0 (en)	2005-08-19	2005-09-28	Enigma Diagnostics Ltd	Analytical method and kit
JP4999341B2 (ja) *	2005-09-06	2012-08-15	株式会社バイオエネックス	変異型ホタルルシフェラーゼ、遺伝子、組換えベクター、形質転換体、及び変異型ホタルルシフェラーゼの製造方法
JP5224457B2 (ja) *	2006-06-12	2013-07-03	独立行政法人産業技術総合研究所	変異型ルシフェラーゼ
JP5188771B2 (ja)	2007-09-26	2013-04-24	株式会社日立製作所	変異型ホタルルシフェラーゼ
JP5753083B2 (ja)	2008-07-22	2015-07-22	プロメガコーポレイションＰｒｏｍｅｇａＣｏｒｐｏｒａｔｉｏｎ	Ａｄｐ検出に基づく発光ホスホトランスフェラーゼまたはａｔｐヒドロラーゼのアッセイ法
JP2011120559A (ja) *	2008-12-26	2011-06-23	Kikkoman Corp	ホタルルシフェラーゼ、その遺伝子、およびホタルルシフェラーゼの製造法
DK2456864T3 (en)	2009-05-01	2015-12-21	Promega Corp	SYNTHETIC OPLOPHORUS luciferases WITH IMPROVED LIGHT MISSION
WO2011002924A2 (en) *	2009-06-30	2011-01-06	The General Hospital Corporation	Gaussia luciferase variant for high-throughput screening
PT2635583E (pt)	2010-11-02	2015-09-11	Promega Corp	Derivados da coelenterazina e métodos de utilização da mesma
US8669103B2 (en)	2010-11-02	2014-03-11	Promega Corporation	Oplophorus-derived luciferases, novel coelenterazine substrates, and methods of use
JP5896679B2 (ja)	2011-03-15	2016-03-30	オリンパス株式会社	オオオバボタル由来ルシフェラーゼ
RU2495929C1 (ru) *	2012-05-30	2013-10-20	ФЕДЕРАЛЬНОЕ ГОСУДАРСТВЕННОЕ БЮДЖЕТНОЕ УЧРЕЖДЕНИЕ НАУКИ Институт биофизики Сибирского отделения Российской академии наук	УКОРОЧЕННАЯ МУТАНТНАЯ ЛЮЦИФЕРАЗА ИЗ Metridia longa ДЛЯ ПРИМЕНЕНИЯ В КАЧЕСТВЕ БИОЛЮМИНЕСЦЕНТНОГО РЕПОРТЕРА В ЖИВЫХ КЛЕТКАХ
US9927430B2 (en)	2014-01-29	2018-03-27	Promega Corporation	Pro-substrates for live cell applications
WO2015116867A1 (en)	2014-01-29	2015-08-06	Promega Corporation	Quinone-masked probes as labeling reagents for cell uptake measurements
WO2016040788A1 (en)	2014-09-11	2016-03-17	Promega Corporation	Luciferase sequences utilizing infrared-emitting substrates to produce enhanced luminescence
US9677117B2 (en)	2014-10-08	2017-06-13	Promega Corporation	Bioluminescent succinate detection assay
RU2674894C2 (ru) *	2017-01-30	2018-12-13	Общество с ограниченной ответственностью "ПЛАНТА"	Новые люциферазы и способы их использования
GB201805396D0 (en) *	2018-04-01	2018-05-16	Univ College Cardiff Consultants Ltd	Modified Luciferases
CN110456052A (zh) *	2019-08-22	2019-11-15	中国科学院武汉病毒研究所	一种血清中梅毒螺旋体抗体的快速检测方法及其应用
WO2021162123A1 (ja)	2020-02-14	2021-08-19	キッコーマン株式会社	試料中のａｔｐ、並びにａｍｐ及び／又はａｄｐを測定するための液体組成物
CN114015664B (zh) *	2021-12-06	2023-07-21	郑州伊美诺生物技术有限公司	荧光素酶突变体及其应用
CN114350627B (zh) *	2021-12-16	2022-08-05	大连博格林生物科技有限公司	一种萤火虫荧光素酶突变体及其制备方法
EP4560025A1 (de)	2022-07-22	2025-05-28	Kikkoman Corporation	Verfahren und kit zum nachweis von mikroorganismen oder zellen oder nachweis von mikroorganismen oder zellverwandten substanzen
CN120457210A (zh)	2023-01-27	2025-08-08	龟甲万株式会社	萤光素酶突变体

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
JP3048466B2 (ja) *	1991-06-27	2000-06-05	キッコーマン株式会社	耐熱性ホタルルシフェラーゼ、耐熱性ホタルルシフェラーゼ遺伝子、新規な組み換え体ｄｎａ、及び耐熱性ホタルルシフェラーゼの製造法
ES2301160T3 (es) *	1994-01-03	2008-06-16	Promega Corporation	Luciferasas mutantes.
GB9501170D0 (en) *	1994-03-23	1995-03-08	Secr Defence	Luciferases
AU707243B2 (en) *	1995-01-20	1999-07-08	Promega Corporation	Mutant luciferases
GB9707486D0 (en) *	1997-04-11	1997-05-28	Secr Defence	Enzyme assays
WO1999014336A2 (en) *	1997-09-19	1999-03-25	Promega Corporation	Thermostable luciferases and methods of production
US6602677B1 (en) *	1997-09-19	2003-08-05	Promega Corporation	Thermostable luciferases and methods of production
GB9823468D0 (en) *	1998-10-28	1998-12-23	Secr Defence	Novel enzyme

2000
- 2000-10-26 AT AT00971589T patent/ATE374820T1/de not_active IP Right Cessation
- 2000-10-26 RU RU2002113653/13A patent/RU2251571C2/ru not_active IP Right Cessation
- 2000-10-26 EP EP00971589A patent/EP1224294B1/de not_active Expired - Lifetime
- 2000-10-26 ES ES00971589T patent/ES2292479T3/es not_active Expired - Lifetime
- 2000-10-26 JP JP2001533163A patent/JP5154724B2/ja not_active Expired - Lifetime
- 2000-10-26 CN CNB008177368A patent/CN100516223C/zh not_active Expired - Fee Related
- 2000-10-26 DE DE60036633T patent/DE60036633T2/de not_active Expired - Lifetime
- 2000-10-26 NZ NZ518413A patent/NZ518413A/en unknown
- 2000-10-26 WO PCT/GB2000/004133 patent/WO2001031028A2/en not_active Ceased
- 2000-10-26 CA CA002387691A patent/CA2387691A1/en not_active Abandoned
- 2000-10-26 AU AU10425/01A patent/AU772485C/en not_active Ceased
- 2000-10-26 DK DK00971589T patent/DK1224294T3/da active

Also Published As

Publication number	Publication date
DE60036633T2 (de)	2008-07-03
EP1224294A2 (de)	2002-07-24
CN100516223C (zh)	2009-07-22
WO2001031028A2 (en)	2001-05-03
AU772485B2 (en)	2004-04-29
ES2292479T3 (es)	2008-03-16
AU1042501A (en)	2001-05-08
AU772485C (en)	2004-11-18
RU2251571C2 (ru)	2005-05-10
CA2387691A1 (en)	2001-05-03
CN1413252A (zh)	2003-04-23
DK1224294T3 (da)	2008-02-04
DE60036633D1 (de)	2007-11-15
JP2003512071A (ja)	2003-04-02
RU2002113653A (ru)	2004-01-27
WO2001031028A3 (en)	2002-05-02
NZ518413A (en)	2004-11-26
JP5154724B2 (ja)	2013-02-27
EP1224294B1 (de)	2007-10-03

Legal Events

Date	Code	Title	Description
2008-04-15	RER	Ceased as to paragraph 5 lit. 3 law introducing patent treaties

Publication	Publication Date	Title
DE60036633D1 (de)	2007-11-15	Mutantenluciferase
ATE418602T1 (de)	2009-01-15	Mutierte luciferase mit verbesserter thermostabilität
DK0973874T3 (da)	2003-05-19	Enzymassay for mutant ildflue-luciferase
DE69535744D1 (de)	2008-05-21	Mutierte luziferasen
CN100387720C (zh)	2008-05-14	虫荧光素酶
DE69928687D1 (en)	2006-01-05	Thermostabile luciferase aus photuris pennsylvanica und pyrophorus plagiophthalamus und hertsellungsmethoden
EP2284271A3 (de)	2012-03-07	Mutierte Luziferase
WO1999066324A3 (en)	2000-04-06	A bioluminescence resonance energy transfer (bret) system and its use
JP2003512071A5 (de)	2007-12-20
ATE472554T1 (de)	2010-07-15	Aminosäureaustauschmutanten von interleukin 13
BR9909012A (pt)	2000-11-28	Enzima para oxidação de fenol purificada, polinucleotìdeo isolado, vetor de expressão, célula hospedeira, processos para produzir uma enzima para oxidação de fenol e para produzir uma célula hospedeira, cultura substancialmente pura, e, composição de enzima
US7807429B2 (en)	2010-10-05	Isolated luciferase gene of L. italica
Wienhausen et al.	1985	Luciferases from different species of fireflies are antigenically similar
Wood	1990	Luciferases of luminous beetles: evolution, color variation, and applications
TR200002713T2 (tr)	2000-12-21	Fenol oksitleyici enzimler ve kullanımları
WO2003044223A3 (en)	2003-08-07	Phosphine-containing formulations for chemiluminescent luciferase assays
Puri et al.	1997	Purification and characterization of dexamethasone inducible hepatic cytochrome P450 isozymes from rhesus monkey
Squirrell et al.	2001	Enzyme assay for mutant firefly luciferase
Jiang et al.	2012	New Cell Biology Assays Using a Stabilized Luciferase Exhibiting Red Luminescence
MCELROY	1988	Firefly luciferase-structure and function(Final Report, 1 Jun. 1986- 31 May 1988)
FR3092587A1 (fr)	2020-08-14	Proteines bioluminescentes aux proprietes ameliorees