ATE376593T1 - ENZYMATIC TREATMENT OF OILS - Google Patents

ENZYMATIC TREATMENT OF OILS

Info

Publication number
ATE376593T1
ATE376593T1 AT04806534T AT04806534T ATE376593T1 AT E376593 T1 ATE376593 T1 AT E376593T1 AT 04806534 T AT04806534 T AT 04806534T AT 04806534 T AT04806534 T AT 04806534T AT E376593 T1 ATE376593 T1 AT E376593T1
Authority
AT
Austria
Prior art keywords
diglyceride
edible oil
glycerol
enzyme
fatty
Prior art date
Application number
AT04806534T
Other languages
German (de)
Inventor
Anna Cecilie Jentof Kristensen
Paul Wassell
Jorn Dalgaard Mikkelsen
Jorn Borch Soe
Original Assignee
Danisco
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from GBGB0330016.7A external-priority patent/GB0330016D0/en
Priority claimed from PCT/IB2004/000655 external-priority patent/WO2004064537A2/en
Priority claimed from US10/898,775 external-priority patent/US7718408B2/en
Application filed by Danisco filed Critical Danisco
Application granted granted Critical
Publication of ATE376593T1 publication Critical patent/ATE376593T1/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6436—Fatty acid esters
    • C12P7/6445—Glycerides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1025—Acyltransferases (2.3)
    • C12N9/1029—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/18—Carboxylic ester hydrolases (3.1.1)
    • C12N9/20—Triglyceride splitting, e.g. by means of lipase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6409—Fatty acids
    • C12P7/6418—Fatty acids by hydrolysis of fatty acid esters
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6436—Fatty acid esters
    • C12P7/6445—Glycerides
    • C12P7/6458—Glycerides by transesterification, e.g. interesterification, ester interchange, alcoholysis or acidolysis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y203/00—Acyltransferases (2.3)
    • C12Y203/01—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
    • C12Y203/01073—Diacylglycerol-sterol O-acyltransferase (2.3.1.73)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y301/00—Hydrolases acting on ester bonds (3.1)
    • C12Y301/01—Carboxylic ester hydrolases (3.1.1)
    • C12Y301/01003—Triacylglycerol lipase (3.1.1.3)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Fats And Perfumes (AREA)

Abstract

The present invention relates to a method of reducing and/or removing diglyceride from an edible oil, comprising a) admixing an edible oil with an acyl acceptor substrate and a fatty-acid CoA independent diglyceride: glycerol acyltransferase, wherein the fatty-acid CoA independent diglyceride: glycerol acyltransferase is characterized as an enzyme which in an edible oil is capable of transferring an acyl group from a diglyceride to glycerol. Preferably, the diglyceride: glycerol acyltransferase comprises the amino acid sequence motif GDSX, wherein X is one or more of the following amino acid residues L, A, V, I, F, Y, H, Q, T, N, M or S. Furthermore the present invention relates to the use of a fatty-acid CoA independent diglyceride: glycerol acyltransferase characterized as an enzyme which in an edible oil is capable of transferring an acyl group from a diglyceride to glycerol, in the manufacture of an edible oil, for reducing and/or removing (preferably selectively reducing and/or removing) diglyceride from said edible oil, and to the use of said enzyme in the manufacture of a foodstuff comprising an edible oil for improving the crystallization properties of said foodstuff.
AT04806534T 2003-12-24 2004-12-23 ENZYMATIC TREATMENT OF OILS ATE376593T1 (en)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
GBGB0330016.7A GB0330016D0 (en) 2003-12-24 2003-12-24 Method
PCT/IB2004/000655 WO2004064537A2 (en) 2003-01-17 2004-01-15 Method for the in situ production of an emulsifier in foodstuff
GBGB0416023.0A GB0416023D0 (en) 2003-12-24 2004-07-16 Method
US10/898,775 US7718408B2 (en) 2003-12-24 2004-07-26 Method

Publications (1)

Publication Number Publication Date
ATE376593T1 true ATE376593T1 (en) 2007-11-15

Family

ID=34753782

Family Applications (1)

Application Number Title Priority Date Filing Date
AT04806534T ATE376593T1 (en) 2003-12-24 2004-12-23 ENZYMATIC TREATMENT OF OILS

Country Status (10)

Country Link
EP (2) EP1862554B1 (en)
CN (2) CN1898386A (en)
AT (1) ATE376593T1 (en)
AU (1) AU2004312213B2 (en)
BR (1) BRPI0418107A (en)
DE (1) DE602004009713T2 (en)
DK (1) DK1704240T3 (en)
ES (1) ES2294575T3 (en)
NZ (1) NZ547082A (en)
WO (1) WO2005066351A2 (en)

Families Citing this family (25)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6936289B2 (en) 1995-06-07 2005-08-30 Danisco A/S Method of improving the properties of a flour dough, a flour dough improving composition and improved food products
ES2168236T3 (en) 1997-04-09 2005-04-16 Danisco A/S USE OF LIPASE TO IMPROVE BREAD PASTA AND BAKERY PRODUCTS.
ATE231186T1 (en) 1998-07-21 2003-02-15 Danisco GROCERIES
BR0209154A (en) 2001-05-18 2004-07-20 Danisco Process of preparing a dough with an enzyme
US20050196766A1 (en) 2003-12-24 2005-09-08 Soe Jorn B. Proteins
US7955814B2 (en) 2003-01-17 2011-06-07 Danisco A/S Method
DE602004030000D1 (en) 2003-01-17 2010-12-23 Danisco PROCESS FOR IN-SITU-PRODUCTION OF AN EMULSIFIER IN A FOODSTUFF
GB0716126D0 (en) 2007-08-17 2007-09-26 Danisco Process
US7718408B2 (en) 2003-12-24 2010-05-18 Danisco A/S Method
WO2008090395A1 (en) 2007-01-25 2008-07-31 Danisco A/S Production of a lipid acyltransferase from transformed bacillus licheniformis cells
US7906307B2 (en) 2003-12-24 2011-03-15 Danisco A/S Variant lipid acyltransferases and methods of making
GB0405637D0 (en) 2004-03-12 2004-04-21 Danisco Protein
CN101052702B (en) 2004-07-16 2013-01-09 杜邦营养生物科学有限公司 Lipolytic Enzyme and Its Application in Food Industry
CN102559627B (en) * 2007-01-25 2014-09-10 杜邦营养生物科学有限公司 Method for utilizing Bacillus licheniformis transformed cell to prepare fatty acyl transferase
WO2008142433A1 (en) 2007-05-24 2008-11-27 Loders Croklaan B.V. Process for producing compositions comprising tocoherols and tocotrienols
GB0812559D0 (en) 2008-07-09 2008-08-13 Danisco Method
EP2367923A2 (en) * 2008-12-01 2011-09-28 Danisco US Inc. Enzymes with lipase activity
CN102656264A (en) * 2009-10-15 2012-09-05 丹尼斯科公司 Lipid acyltransferase proteins and methods of making them
GB0920089D0 (en) 2009-11-17 2009-12-30 Danisco Method
EP2395070A1 (en) * 2010-06-10 2011-12-14 The Procter & Gamble Company Liquid laundry detergent composition comprising lipase of bacterial origin
EP2395071A1 (en) * 2010-06-10 2011-12-14 The Procter & Gamble Company Solid detergent composition comprising lipase of bacterial origin
MY181371A (en) 2012-05-10 2020-12-21 Nisshin Oillio Group Ltd Oil and fat composition suitable for non-tempering hard butter
KR20220077128A (en) 2019-09-10 2022-06-08 퍼펙트 데이, 인크. Compositions comprising a subset of milk lipids, and methods of producing the same
WO2021167515A1 (en) * 2020-02-20 2021-08-26 Aak Ab (Publ) Food product comprising a vegetable fat composition with a low amount of diglycerides
AU2021223013A1 (en) * 2020-02-20 2022-08-11 Aak Ab (Publ) Fat composition suitable as a cocoa butter equivalent having a low amount of diglycerides

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US5219744A (en) * 1987-08-26 1993-06-15 Ajinomoto Co., Inc. Process for modifying fats and oils
DK1599278T3 (en) * 2003-01-17 2011-07-18 Danisco Process for preparing a hydroxy acid ester
BRPI0417533B1 (en) * 2003-12-24 2016-03-01 Danisco variant enzyme glycolipid acyltransferase, use and method for its production, method for preparing a food product and flour baked product, enzymatic refinement process

Also Published As

Publication number Publication date
AU2004312213A1 (en) 2005-07-21
BRPI0418107A (en) 2007-04-17
EP1862554A3 (en) 2007-12-19
EP1704240B1 (en) 2007-10-24
ES2294575T3 (en) 2008-04-01
WO2005066351A2 (en) 2005-07-21
HK1091868A1 (en) 2007-01-26
AU2004312213B2 (en) 2010-03-11
EP1862554A2 (en) 2007-12-05
CN1898391A (en) 2007-01-17
WO2005066351A3 (en) 2006-03-23
DK1704240T3 (en) 2008-02-25
EP1704240A2 (en) 2006-09-27
NZ547082A (en) 2009-07-31
CN1898386A (en) 2007-01-17
DE602004009713D1 (en) 2007-12-06
EP1862554B1 (en) 2010-03-24
DE602004009713T2 (en) 2008-03-13

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