ATE382100T1 - Nukleinsaure vervielfaeltigungsverfahren - Google Patents

Nukleinsaure vervielfaeltigungsverfahren

Info

Publication number
ATE382100T1
ATE382100T1 AT03763984T AT03763984T ATE382100T1 AT E382100 T1 ATE382100 T1 AT E382100T1 AT 03763984 T AT03763984 T AT 03763984T AT 03763984 T AT03763984 T AT 03763984T AT E382100 T1 ATE382100 T1 AT E382100T1
Authority
AT
Austria
Prior art keywords
fap
rna
tap
sequence
heeled
Prior art date
Application number
AT03763984T
Other languages
English (en)
Inventor
Alastair Dixon
Michael Skynner
Original Assignee
Cambridge Biotechnology Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Cambridge Biotechnology Ltd filed Critical Cambridge Biotechnology Ltd
Application granted granted Critical
Publication of ATE382100T1 publication Critical patent/ATE382100T1/de

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1096Processes for the isolation, preparation or purification of DNA or RNA cDNA Synthesis; Subtracted cDNA library construction, e.g. RT, RT-PCR
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
    • C12Q1/6853Nucleic acid amplification reactions using modified primers or templates
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10Modifications characterised by
    • C12Q2525/131Modifications characterised by incorporating a restriction site
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00Oligonucleotides characterized by their use
    • C12Q2600/158Expression markers

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biophysics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Immunology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Plant Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Steroid Compounds (AREA)
AT03763984T 2002-07-10 2003-07-10 Nukleinsaure vervielfaeltigungsverfahren ATE382100T1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
GBGB0216026.5A GB0216026D0 (en) 2002-07-10 2002-07-10 Nucleic acid amplification method

Publications (1)

Publication Number Publication Date
ATE382100T1 true ATE382100T1 (de) 2008-01-15

Family

ID=9940217

Family Applications (1)

Application Number Title Priority Date Filing Date
AT03763984T ATE382100T1 (de) 2002-07-10 2003-07-10 Nukleinsaure vervielfaeltigungsverfahren

Country Status (10)

Country Link
US (1) US20060240420A1 (de)
EP (1) EP1520049B1 (de)
JP (1) JP2005532074A (de)
AT (1) ATE382100T1 (de)
AU (1) AU2003254445B2 (de)
CA (1) CA2491984A1 (de)
DE (1) DE60318315T2 (de)
GB (1) GB0216026D0 (de)
WO (1) WO2004007766A1 (de)
ZA (1) ZA200500201B (de)

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB9817055D0 (en) * 1998-08-05 1998-09-30 Medical Res Council Reverse transcription and amplification processes and primers therefore
JP2003505034A (ja) * 1999-07-19 2003-02-12 ケンブリッジ・ユニバーシティ・テクニカル・サービシーズ・リミテッド 存在量の少ない核酸配列を増幅する方法および該方法を行うための手段
US6436677B1 (en) * 2000-03-02 2002-08-20 Promega Corporation Method of reverse transcription
EP1275738A1 (de) * 2001-07-11 2003-01-15 Roche Diagnostics GmbH Verfahren zur zufällige Synthese und Amplifikation komplementäres DNA

Also Published As

Publication number Publication date
CA2491984A1 (en) 2004-01-22
EP1520049B1 (de) 2007-12-26
DE60318315T2 (de) 2008-12-11
WO2004007766A1 (en) 2004-01-22
AU2003254445B2 (en) 2006-06-15
ZA200500201B (en) 2006-07-26
US20060240420A1 (en) 2006-10-26
AU2003254445A1 (en) 2004-02-02
GB0216026D0 (en) 2002-08-21
DE60318315D1 (de) 2008-02-07
JP2005532074A (ja) 2005-10-27
EP1520049A1 (de) 2005-04-06

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Legal Events

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