ATE382687T1 - Verfahren zur stellengerichteten mutagenese und gezielten randomisierung - Google Patents
Verfahren zur stellengerichteten mutagenese und gezielten randomisierungInfo
- Publication number
- ATE382687T1 ATE382687T1 AT04703064T AT04703064T ATE382687T1 AT E382687 T1 ATE382687 T1 AT E382687T1 AT 04703064 T AT04703064 T AT 04703064T AT 04703064 T AT04703064 T AT 04703064T AT E382687 T1 ATE382687 T1 AT E382687T1
- Authority
- AT
- Austria
- Prior art keywords
- site
- directed mutagenesis
- targeted randomization
- randomization
- targeted
- Prior art date
Links
- 238000000034 method Methods 0.000 title abstract 3
- 238000002741 site-directed mutagenesis Methods 0.000 title 1
- 241000193830 Bacillus <bacterium> Species 0.000 abstract 2
- 241000588724 Escherichia coli Species 0.000 abstract 1
- 238000010276 construction Methods 0.000 abstract 1
- 239000000203 mixture Substances 0.000 abstract 1
- 102000004169 proteins and genes Human genes 0.000 abstract 1
- 108090000623 proteins and genes Proteins 0.000 abstract 1
- 230000009466 transformation Effects 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
- C12N15/75—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Bacillus
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/102—Mutagenizing nucleic acids
Landscapes
- Genetics & Genomics (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Wood Science & Technology (AREA)
- Organic Chemistry (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biophysics (AREA)
- Microbiology (AREA)
- Plant Pathology (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Physics & Mathematics (AREA)
- Crystallography & Structural Chemistry (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Medicines Containing Plant Substances (AREA)
- Compounds Of Unknown Constitution (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US44079203P | 2003-01-16 | 2003-01-16 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ATE382687T1 true ATE382687T1 (de) | 2008-01-15 |
Family
ID=32771864
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AT04703064T ATE382687T1 (de) | 2003-01-16 | 2004-01-16 | Verfahren zur stellengerichteten mutagenese und gezielten randomisierung |
Country Status (7)
| Country | Link |
|---|---|
| US (1) | US20060252155A1 (de) |
| EP (1) | EP1587924B2 (de) |
| AT (1) | ATE382687T1 (de) |
| CA (1) | CA2513227C (de) |
| DE (1) | DE602004011017T3 (de) |
| DK (1) | DK1587924T4 (de) |
| WO (1) | WO2004064744A2 (de) |
Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| MX2011004801A (es) | 2008-11-11 | 2011-06-16 | Danisco Inc | Composiciones y metodos que comprenden una variante de subtilisina. |
| WO2010056671A1 (en) | 2008-11-11 | 2010-05-20 | Danisco Us Inc. | Compositions and methods comprising a subtilisin variant |
| PL2558573T3 (pl) | 2010-04-15 | 2017-08-31 | Danisco Us Inc. | Kompozycje i sposoby obejmujące warianty proteazy |
| EP4525615A2 (de) | 2022-05-14 | 2025-03-26 | Novozymes A/S | Zusammensetzungen und verfahren zur prävention, behandlung, unterdrückung und/oder beseitigung von phytopathogenen befällen und infektionen |
Family Cites Families (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5182204A (en) * | 1984-05-29 | 1993-01-26 | Genencor International, Inc. | Non-human carbonyl hydrolase mutants, vectors encoding same and hosts transformed with said vectors |
| US5418873A (en) * | 1993-09-09 | 1995-05-23 | Digisonix, Inc. | Active acoustic attenuation system with indirect error sensing |
| US5693506A (en) | 1993-11-16 | 1997-12-02 | The Regents Of The University Of California | Process for protein production in plants |
| US5776746A (en) * | 1996-05-01 | 1998-07-07 | Genitope Corporation | Gene amplification methods |
| US20030162209A1 (en) * | 2001-12-19 | 2003-08-28 | George Martin | PCR based high throughput polypeptide screening |
-
2004
- 2004-01-16 CA CA2513227A patent/CA2513227C/en not_active Expired - Lifetime
- 2004-01-16 AT AT04703064T patent/ATE382687T1/de not_active IP Right Cessation
- 2004-01-16 EP EP04703064A patent/EP1587924B2/de not_active Expired - Lifetime
- 2004-01-16 WO PCT/US2004/001334 patent/WO2004064744A2/en not_active Ceased
- 2004-01-16 DK DK04703064.8T patent/DK1587924T4/da active
- 2004-01-16 US US10/541,737 patent/US20060252155A1/en not_active Abandoned
- 2004-01-16 DE DE602004011017T patent/DE602004011017T3/de not_active Expired - Lifetime
Also Published As
| Publication number | Publication date |
|---|---|
| CA2513227A1 (en) | 2004-08-05 |
| DK1587924T4 (da) | 2012-04-02 |
| DE602004011017T3 (de) | 2012-06-06 |
| WO2004064744A3 (en) | 2004-11-04 |
| DK1587924T3 (da) | 2008-05-13 |
| WO2004064744A2 (en) | 2004-08-05 |
| US20060252155A1 (en) | 2006-11-09 |
| EP1587924A2 (de) | 2005-10-26 |
| EP1587924A4 (de) | 2006-05-10 |
| DE602004011017T2 (de) | 2008-12-24 |
| EP1587924B2 (de) | 2012-01-18 |
| DE602004011017D1 (de) | 2008-02-14 |
| EP1587924B1 (de) | 2008-01-02 |
| CA2513227C (en) | 2012-09-11 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| RER | Ceased as to paragraph 5 lit. 3 law introducing patent treaties |