ATE389022T1 - Verstärkte herstellung rekombinanter proteine mittels transienter transfektion von in suspension wachsenden säugerzellen - Google Patents
Verstärkte herstellung rekombinanter proteine mittels transienter transfektion von in suspension wachsenden säugerzellenInfo
- Publication number
- ATE389022T1 ATE389022T1 AT02727108T AT02727108T ATE389022T1 AT E389022 T1 ATE389022 T1 AT E389022T1 AT 02727108 T AT02727108 T AT 02727108T AT 02727108 T AT02727108 T AT 02727108T AT E389022 T1 ATE389022 T1 AT E389022T1
- Authority
- AT
- Austria
- Prior art keywords
- recombinant proteins
- protein
- suspension
- transfection
- expression
- Prior art date
Links
- 102000007056 Recombinant Fusion Proteins Human genes 0.000 title abstract 3
- 108010008281 Recombinant Fusion Proteins Proteins 0.000 title abstract 3
- 238000004519 manufacturing process Methods 0.000 title abstract 3
- 238000003146 transient transfection Methods 0.000 title abstract 2
- 241000124008 Mammalia Species 0.000 title 1
- 239000000725 suspension Substances 0.000 title 1
- 238000000034 method Methods 0.000 abstract 5
- 241000701044 Human gammaherpesvirus 4 Species 0.000 abstract 2
- 229920002873 Polyethylenimine Polymers 0.000 abstract 2
- 210000004027 cell Anatomy 0.000 abstract 2
- 239000013604 expression vector Substances 0.000 abstract 2
- 239000001963 growth medium Substances 0.000 abstract 2
- 241000701022 Cytomegalovirus Species 0.000 abstract 1
- 108010031111 EBV-encoded nuclear antigen 1 Proteins 0.000 abstract 1
- 230000010261 cell growth Effects 0.000 abstract 1
- 238000010924 continuous production Methods 0.000 abstract 1
- 230000002068 genetic effect Effects 0.000 abstract 1
- 210000003292 kidney cell Anatomy 0.000 abstract 1
- 108090000623 proteins and genes Proteins 0.000 abstract 1
- 102000004169 proteins and genes Human genes 0.000 abstract 1
- 238000001890 transfection Methods 0.000 abstract 1
- 239000012096 transfection reagent Substances 0.000 abstract 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0684—Cells of the urinary tract or kidneys
- C12N5/0686—Kidney cells
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
- C07K14/01—DNA viruses
- C07K14/03—Herpetoviridae, e.g. pseudorabies virus
- C07K14/05—Epstein-Barr virus
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2500/00—Specific components of cell culture medium
- C12N2500/90—Serum-free medium, which may still contain naturally-sourced components
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2510/00—Genetically modified cells
- C12N2510/02—Cells for production
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2710/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
- C12N2710/00011—Details
- C12N2710/16011—Herpesviridae
- C12N2710/16111—Cytomegalovirus, e.g. human herpesvirus 5
- C12N2710/16122—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2800/00—Nucleic acids vectors
- C12N2800/10—Plasmid DNA
- C12N2800/108—Plasmid DNA episomal vectors
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2840/00—Vectors comprising a special translation-regulating system
- C12N2840/20—Vectors comprising a special translation-regulating system translation of more than one cistron
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2840/00—Vectors comprising a special translation-regulating system
- C12N2840/20—Vectors comprising a special translation-regulating system translation of more than one cistron
- C12N2840/203—Vectors comprising a special translation-regulating system translation of more than one cistron having an IRES
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2840/00—Vectors comprising a special translation-regulating system
- C12N2840/44—Vectors comprising a special translation-regulating system being a specific part of the splice mechanism, e.g. donor, acceptor
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Genetics & Genomics (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Biotechnology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biomedical Technology (AREA)
- General Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Urology & Nephrology (AREA)
- Virology (AREA)
- Biophysics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Physics & Mathematics (AREA)
- Cell Biology (AREA)
- Plant Pathology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Gastroenterology & Hepatology (AREA)
- Medicinal Chemistry (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US28879001P | 2001-05-07 | 2001-05-07 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ATE389022T1 true ATE389022T1 (de) | 2008-03-15 |
Family
ID=23108658
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AT02727108T ATE389022T1 (de) | 2001-05-07 | 2002-05-07 | Verstärkte herstellung rekombinanter proteine mittels transienter transfektion von in suspension wachsenden säugerzellen |
Country Status (7)
| Country | Link |
|---|---|
| US (2) | US10421950B2 (de) |
| EP (1) | EP1390511B1 (de) |
| AT (1) | ATE389022T1 (de) |
| AU (1) | AU2002257440A1 (de) |
| CA (1) | CA2446185C (de) |
| DE (1) | DE60225545T2 (de) |
| WO (1) | WO2002090533A2 (de) |
Families Citing this family (23)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US7217566B2 (en) * | 2003-03-24 | 2007-05-15 | Invitrogen Corporation | Attached cell lines |
| JP2006121995A (ja) * | 2004-10-29 | 2006-05-18 | Tokyo Medical & Dental Univ | Ｅｂｎａ１変異体 |
| CA2578908C (en) * | 2005-03-17 | 2020-05-26 | National Research Council Of Canada | Expression vectors for enhanced transient gene expression and mammalian cells expressing them |
| CA2624685C (en) | 2005-10-28 | 2014-06-03 | Ulrich Goepfert | Protein expression in rodent cells |
| US8168181B2 (en) | 2006-02-13 | 2012-05-01 | Alethia Biotherapeutics, Inc. | Methods of impairing osteoclast differentiation using antibodies that bind siglec-15 |
| CA2638823A1 (en) | 2006-02-13 | 2007-08-23 | Alethia Biotherapeutics Inc. | Polynucleotides and polypeptide sequences involved in the process of bone remodeling |
| US20100159512A1 (en) * | 2006-03-06 | 2010-06-24 | Kurt Osther | Method for the preparation of recombinant human thrombin and fibrinogen |
| US8076139B1 (en) | 2006-05-19 | 2011-12-13 | Leinco Technologies, Inc. | Processes and compositions for transfecting Chinese hamster ovary (CHO) cells |
| WO2015164330A1 (en) | 2014-04-21 | 2015-10-29 | Millennium Pharmaceuticals, Inc. | Anti-psyk antibody molecules and use of same for syk-targeted therapy |
| EP2631297B1 (de) | 2008-05-15 | 2017-04-12 | National Research Council Of Canada | Verfahren, Vektoren und manipulierte Zelllinien für Transfektion in grossem Massstab |
| US20110165620A1 (en) * | 2008-05-28 | 2011-07-07 | Roland Romijn | Method for the production of proteins or protein fragments |
| WO2010060186A1 (en) | 2008-11-03 | 2010-06-03 | Alethia Biotherapeutics Inc. | Antibodies that specifically block the biological activity of a tumor antigen |
| JP6294221B2 (ja) | 2011-03-31 | 2018-03-14 | アー・デー・ツェー・セラピューティクス・エス・アー | 腎臓関連抗原１に対する抗体およびその抗原結合性フラグメント |
| CN103620034B (zh) | 2011-04-13 | 2016-12-14 | 加拿大国家研究委员会 | 具有来自IFNα2的SAR元件的表达系统 |
| TR201908872T4 (tr) | 2012-01-09 | 2019-07-22 | Adc Therapeutics Sa | Üçlü negatif meme kanserini tedavi etmek için ajanlar. |
| ES2723885T3 (es) | 2012-07-19 | 2019-09-03 | Daiichi Sankyo Co Ltd | Anticuerpos anti-Siglec-15 |
| ES2728936T3 (es) | 2013-01-25 | 2019-10-29 | Amgen Inc | Anticuerpos dirigidos contra CDH19 para melanoma |
| FR3025107B1 (fr) | 2014-08-29 | 2018-10-05 | Calixar | Procede de preparation d'un antigene vaccinal, antigene vaccinal obtenu et utilisations |
| EP3548890B1 (de) | 2016-12-01 | 2024-03-27 | Norbert Gretz | Mittel und verfahren zur visualisierung von gewebestrukturen |
| FR3063730B1 (fr) | 2017-03-09 | 2021-09-17 | Centre Nat Rech Scient | Composes-clips surfactants pour l'extraction et la stabilisation en solution de proteines membranaires |
| CN113025648B (zh) * | 2019-12-09 | 2023-06-02 | 广东菲鹏制药股份有限公司 | Expi293细胞瞬时表达目标蛋白的方法及其应用 |
| CN114410682B (zh) * | 2022-02-08 | 2024-07-26 | 美康生物科技股份有限公司 | 一种293t细胞瞬时表达目标蛋白的方法及其应用 |
| CN117265049A (zh) * | 2023-09-12 | 2023-12-22 | 武汉爱博泰克生物科技有限公司 | 利用反应管培养细胞表达高通量重组抗体的方法及装置 |
Family Cites Families (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5804374A (en) * | 1980-12-05 | 1998-09-08 | Massachusetts Insti. Technology | Nuclear factors associates with transcriptional regulation |
| US5550036A (en) * | 1986-04-09 | 1996-08-27 | Eli Lilly And Company | Method for co-amplification of human protein C genes in human cells |
| US5024939A (en) * | 1987-07-09 | 1991-06-18 | Genentech, Inc. | Transient expression system for producing recombinant protein |
| US5024947A (en) * | 1987-07-24 | 1991-06-18 | Cetus Corporation | Serum free media for the growth on insect cells and expression of products thereby |
| DE4138621A1 (de) * | 1991-11-25 | 1993-06-17 | Boehringer Ingelheim Int | Verfahren zum screenen von substanzen mit modulierender wirkung auf einen rezeptorabhaengigen zellulaeren signaluebertragungsweg |
| AU704218B2 (en) * | 1994-05-20 | 1999-04-15 | Board Of Trustees Of The Leland Stanford Junior University | An autonomous replication system for mammalian cells |
| WO1998037185A2 (en) * | 1997-02-20 | 1998-08-27 | The Board Of Regents Of The University Of Texas System | Vectors for controlled gene expression |
| CN1263861C (zh) * | 1997-06-19 | 2006-07-12 | 综合医院公司 | 压缩的eb病毒复制子 |
| US6210922B1 (en) * | 1998-11-30 | 2001-04-03 | National Research Council Of Canada | Serum free production of recombinant proteins and adenoviral vectors |
| US7723102B2 (en) * | 2000-09-28 | 2010-05-25 | Bayer Corporation | Enhanced transfection system |
-
2002
- 2002-05-07 AT AT02727108T patent/ATE389022T1/de not_active IP Right Cessation
- 2002-05-07 AU AU2002257440A patent/AU2002257440A1/en not_active Abandoned
- 2002-05-07 US US10/477,148 patent/US10421950B2/en active Active
- 2002-05-07 WO PCT/CA2002/000683 patent/WO2002090533A2/en not_active Ceased
- 2002-05-07 DE DE60225545T patent/DE60225545T2/de not_active Expired - Lifetime
- 2002-05-07 CA CA2446185A patent/CA2446185C/en not_active Expired - Lifetime
- 2002-05-07 EP EP02727108A patent/EP1390511B1/de not_active Expired - Lifetime
-
2019
- 2019-08-27 US US16/552,093 patent/US10858629B2/en not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| CA2446185A1 (en) | 2002-11-14 |
| WO2002090533A2 (en) | 2002-11-14 |
| EP1390511B1 (de) | 2008-03-12 |
| DE60225545D1 (de) | 2008-04-24 |
| US20200010808A1 (en) | 2020-01-09 |
| AU2002257440A1 (en) | 2002-11-18 |
| DE60225545T2 (de) | 2009-04-02 |
| US20050170450A1 (en) | 2005-08-04 |
| CA2446185C (en) | 2013-06-18 |
| EP1390511A2 (de) | 2004-02-25 |
| WO2002090533A9 (en) | 2002-12-27 |
| US10858629B2 (en) | 2020-12-08 |
| WO2002090533A3 (en) | 2003-05-22 |
| US10421950B2 (en) | 2019-09-24 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| RER | Ceased as to paragraph 5 lit. 3 law introducing patent treaties |