ATE391173T1 - Thermostabile ligase aus thermus phage - Google Patents

Thermostabile ligase aus thermus phage

Info

Publication number
ATE391173T1
ATE391173T1 AT03797506T AT03797506T ATE391173T1 AT E391173 T1 ATE391173 T1 AT E391173T1 AT 03797506 T AT03797506 T AT 03797506T AT 03797506 T AT03797506 T AT 03797506T AT E391173 T1 ATE391173 T1 AT E391173T1
Authority
AT
Austria
Prior art keywords
thermostable ligase
thermus
phage
nucleic acids
thermus phage
Prior art date
Application number
AT03797506T
Other languages
English (en)
Inventor
Sigridur Hjorleifsdottir
Audur Thorisdottir
Arnthor Aevarsson
Gudmundur Hreggvidsson
Thorarinn Blondal
Original Assignee
Prokaria Ehf
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Prokaria Ehf filed Critical Prokaria Ehf
Application granted granted Critical
Publication of ATE391173T1 publication Critical patent/ATE391173T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/93—Ligases (6)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/30—Nucleotides
    • C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
    • C12Q1/6855—Ligating adaptors
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2795/00—Bacteriophages
    • C12N2795/00011—Details
    • C12N2795/10011—Details dsDNA Bacteriophages
    • C12N2795/10022—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biomedical Technology (AREA)
  • Virology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
AT03797506T 2002-09-20 2003-09-19 Thermostabile ligase aus thermus phage ATE391173T1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US41246102P 2002-09-20 2002-09-20

Publications (1)

Publication Number Publication Date
ATE391173T1 true ATE391173T1 (de) 2008-04-15

Family

ID=32030871

Family Applications (1)

Application Number Title Priority Date Filing Date
AT03797506T ATE391173T1 (de) 2002-09-20 2003-09-19 Thermostabile ligase aus thermus phage

Country Status (6)

Country Link
US (1) US7303901B2 (de)
EP (1) EP1546313B1 (de)
AT (1) ATE391173T1 (de)
AU (1) AU2003260946A1 (de)
DE (1) DE60320119D1 (de)
WO (1) WO2004027054A1 (de)

Families Citing this family (23)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2006219739A1 (en) * 2005-03-04 2006-09-08 Blaze Venture Technologies Limited Method and device for bacterial sampling
WO2009009139A2 (en) * 2007-07-11 2009-01-15 The General Hospital Corporation Rna ligase polypeptides and methods of selection and use thereof
EP2285953B1 (de) 2008-05-02 2015-01-14 Cellscript, Llc Rna-polyphosphatasezusammensetzungen, kits und ihre verwendung
CN102076851A (zh) * 2008-05-02 2011-05-25 Epi中心科技公司 Rna的选择性的5′连接标记
US8583380B2 (en) 2008-09-05 2013-11-12 Aueon, Inc. Methods for stratifying and annotating cancer drug treatment options
DK2787565T3 (en) 2008-10-24 2015-10-12 Epict Technologies Corp Transposon-end COMPOSITIONS AND METHODS TO MODIFY nucleic acids
US9080211B2 (en) 2008-10-24 2015-07-14 Epicentre Technologies Corporation Transposon end compositions and methods for modifying nucleic acids
AU2010213510B2 (en) * 2009-02-16 2014-05-01 Epicentre Technologies Corporation Template-independent ligation of single-stranded DNA
WO2011056866A2 (en) * 2009-11-05 2011-05-12 Epicentre Technologies Corporation Methods and kits for 3'-end-tagging of rna
US20130143276A1 (en) * 2010-04-01 2013-06-06 New England Biolabs, Inc. Compositions and Methods for Adenylating Oligonucleotides
CN103228798B (zh) 2010-09-24 2015-12-09 斯坦福大学托管董事会 使用固定的引物直接捕获、扩增及测序靶标dna
EP3567121B1 (de) 2011-01-17 2023-08-30 Life Technologies Corporation Arbeitsablauf zum nachweis von liganden mit nukleinsäuren
CN106029962A (zh) * 2014-02-11 2016-10-12 豪夫迈·罗氏有限公司 靶向测序和uid过滤
EP3303377B1 (de) 2015-06-05 2022-11-23 Dana-Farber Cancer Institute, Inc. Zusammensetzungen und verfahren für vorübergehende gentherapie mit verbesserter stabilität
WO2017160788A2 (en) * 2016-03-14 2017-09-21 RGENE, Inc. HYPER-THERMOSTABLE LYSINE-MUTANT ssDNA/RNA LIGASES
WO2017210469A2 (en) 2016-06-01 2017-12-07 F. Hoffman-La Roche Ag Immuno-pete
TWI699435B (zh) 2016-07-01 2020-07-21 體學生物科技股份有限公司 建構環狀模板和檢測dna分子的方法
US11091791B2 (en) 2017-02-24 2021-08-17 Mgi Tech Co., Ltd. Methods for hybridization based hook ligation
US11753434B2 (en) 2017-04-14 2023-09-12 Dana-Farber Cancer Institute, Inc. Compositions and methods for transient gene therapy with enhanced stability
EP3730613A4 (de) 2017-11-09 2021-11-03 MGI Tech Co., Ltd. Hakensonde, verfahren zum binden von nukleinsäure und verfahren zur konstruktion einer sequenzierungsbibliothek
WO2021224677A1 (en) 2020-05-05 2021-11-11 Akershus Universitetssykehus Hf Compositions and methods for characterizing bowel cancer
US20230340449A1 (en) * 2020-07-17 2023-10-26 Qiagen Beverly Llc Thermostable ligase with reduced sequence bias
CN114836401A (zh) * 2022-04-24 2022-08-02 中国人民解放军海军军医大学 重组ssDNA核酸环化酶、制备方法及应用

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5516664A (en) * 1992-12-23 1996-05-14 Hyman; Edward D. Enzymatic synthesis of repeat regions of oligonucleotides
US6492161B1 (en) * 1999-06-02 2002-12-10 Prokaria Ltd. Bacteriophage RM 378 of a thermophilic host organism
JP4310599B2 (ja) * 2000-07-05 2009-08-12 東洋紡績株式会社 塩基多型を検出する方法

Also Published As

Publication number Publication date
EP1546313A1 (de) 2005-06-29
AU2003260946A1 (en) 2004-04-08
US20050266439A1 (en) 2005-12-01
US7303901B2 (en) 2007-12-04
DE60320119D1 (en) 2008-05-15
EP1546313B1 (de) 2008-04-02
WO2004027054A1 (en) 2004-04-01

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