ATE392484T1 - Verbessertes verfahren zur membranpotentialmessung - Google Patents
Verbessertes verfahren zur membranpotentialmessungInfo
- Publication number
- ATE392484T1 ATE392484T1 AT02759289T AT02759289T ATE392484T1 AT E392484 T1 ATE392484 T1 AT E392484T1 AT 02759289 T AT02759289 T AT 02759289T AT 02759289 T AT02759289 T AT 02759289T AT E392484 T1 ATE392484 T1 AT E392484T1
- Authority
- AT
- Austria
- Prior art keywords
- probes
- membrane potential
- improved method
- potential measurement
- fluorescent
- Prior art date
Links
- 238000000034 method Methods 0.000 title abstract 2
- 238000003266 membrane potential measurement method Methods 0.000 title 1
- 239000000523 sample Substances 0.000 abstract 4
- 239000012528 membrane Substances 0.000 abstract 2
- OZLGRUXZXMRXGP-UHFFFAOYSA-N Fluo-3 Chemical compound CC1=CC=C(N(CC(O)=O)CC(O)=O)C(OCCOC=2C(=CC=C(C=2)C2=C3C=C(Cl)C(=O)C=C3OC3=CC(O)=C(Cl)C=C32)N(CC(O)=O)CC(O)=O)=C1 OZLGRUXZXMRXGP-UHFFFAOYSA-N 0.000 abstract 1
- 150000001875 compounds Chemical class 0.000 abstract 1
- 238000012632 fluorescent imaging Methods 0.000 abstract 1
- 239000003269 fluorescent indicator Substances 0.000 abstract 1
- YFHXZQPUBCBNIP-UHFFFAOYSA-N fura-2 Chemical compound CC1=CC=C(N(CC(O)=O)CC(O)=O)C(OCCOC=2C(=CC=3OC(=CC=3C=2)C=2OC(=CN=2)C(O)=O)N(CC(O)=O)CC(O)=O)=C1 YFHXZQPUBCBNIP-UHFFFAOYSA-N 0.000 abstract 1
- PNDZEEPOYCVIIY-UHFFFAOYSA-N indo-1 Chemical compound CC1=CC=C(N(CC(O)=O)CC(O)=O)C(OCCOC=2C(=CC=C(C=2)C=2N=C3[CH]C(=CC=C3C=2)C(O)=O)N(CC(O)=O)CC(O)=O)=C1 PNDZEEPOYCVIIY-UHFFFAOYSA-N 0.000 abstract 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/52—Use of compounds or compositions for colorimetric, spectrophotometric or fluorometric investigation, e.g. use of reagent paper and including single- and multilayer analytical elements
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S435/00—Chemistry: molecular biology and microbiology
- Y10S435/975—Kit
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Hematology (AREA)
- Immunology (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Chemical & Material Sciences (AREA)
- Urology & Nephrology (AREA)
- Food Science & Technology (AREA)
- General Physics & Mathematics (AREA)
- Cell Biology (AREA)
- Biotechnology (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Pathology (AREA)
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating Or Analyzing Materials By The Use Of Electric Means (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Fuel Cell (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US09/924,797 US6852504B2 (en) | 2001-08-08 | 2001-08-08 | Method for measuring cellular transmembrane potential changes |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ATE392484T1 true ATE392484T1 (de) | 2008-05-15 |
Family
ID=25450742
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AT02759289T ATE392484T1 (de) | 2001-08-08 | 2002-08-07 | Verbessertes verfahren zur membranpotentialmessung |
Country Status (6)
| Country | Link |
|---|---|
| US (2) | US6852504B2 (de) |
| EP (1) | EP1421206B1 (de) |
| AT (1) | ATE392484T1 (de) |
| CA (1) | CA2456115C (de) |
| DE (1) | DE60226151T2 (de) |
| WO (1) | WO2003014701A2 (de) |
Families Citing this family (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6852504B2 (en) * | 2001-08-08 | 2005-02-08 | Molecular Devices Corporation | Method for measuring cellular transmembrane potential changes |
| US8329390B2 (en) * | 2004-10-21 | 2012-12-11 | Anaspec Incorporated | Detection of transmembrane potentials using N,N,N′-trialkyl thiobarbituric acid-derived polymethine oxonols |
| DE102007012029A1 (de) * | 2007-03-13 | 2008-09-18 | Grünenthal GmbH | Verfahren zur Identifizierung der agonistischen Aktivität einer Zielverbindung auf einen Kaliumkanal |
| EP2142498A2 (de) * | 2007-04-02 | 2010-01-13 | Institute for Oneworld Health | Cftr-hemmer-verbindungen und verwendungen davon |
| WO2009131957A2 (en) * | 2008-04-21 | 2009-10-29 | Institute For Oneworld Health | Compounds, compositions and methods comprising oxadiazole derivatives |
| US8236838B2 (en) * | 2008-04-21 | 2012-08-07 | Institute For Oneworld Health | Compounds, compositions and methods comprising isoxazole derivatives |
| US20090270398A1 (en) * | 2008-04-21 | 2009-10-29 | Institute For Oneworld Health | Compounds, Compositions and Methods Comprising Pyridazine Derivatives |
| US20090264433A1 (en) * | 2008-04-21 | 2009-10-22 | Institute For Oneworld Health | Compounds, Compositions and Methods Comprising Triazine Derivatives |
| WO2009131948A2 (en) * | 2008-04-21 | 2009-10-29 | Institute For Oneworld Health | High-throughput cell-based cftr assay |
| JP2012503005A (ja) * | 2008-09-19 | 2012-02-02 | インスティテュート フォア ワンワールド ヘルス | イミダゾール誘導体およびトリアゾール誘導体を含む、化合物、組成物ならびに方法。 |
| US8511216B2 (en) * | 2009-03-30 | 2013-08-20 | Kanzaki Kokyukoki Mfg. Co., Ltd. | Hydraulic actuator unit |
| US8343976B2 (en) * | 2009-04-20 | 2013-01-01 | Institute For Oneworld Health | Compounds, compositions and methods comprising pyrazole derivatives |
| CN112945924A (zh) * | 2021-02-03 | 2021-06-11 | 南通大学 | 一种高通量筛选KSper缺陷不育人精子样本的方法 |
Family Cites Families (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE1597482B2 (de) * | 1967-09-22 | 1977-03-24 | Agfa-Gevaert Ag, 5090 Leverkusen | Lichtempfindliches photographisches aufzeichnungsmaterial mit lichthofschutz- oder filterfarbstoffen |
| US4370401A (en) * | 1979-12-07 | 1983-01-25 | Minnesota Mining And Manufacturing Company | Light sensitive, thermally developable imaging system |
| GB2136590B (en) * | 1983-03-15 | 1986-01-02 | Minnesota Mining & Mfg | Dye-bleach materials and process |
| US6342379B1 (en) * | 1995-06-07 | 2002-01-29 | The Regents Of The University Of California | Detection of transmembrane potentials by optical methods |
| US6596522B2 (en) * | 1997-05-08 | 2003-07-22 | The Regents Of The University Of California | Detection of transmembrane potentials by optical methods |
| US6143516A (en) * | 1998-09-25 | 2000-11-07 | Xoma Technology Ltd. | Identification of novel antimicrobial agents using membrane potential indicator dyes |
| WO2001027253A1 (en) * | 1999-10-08 | 2001-04-19 | Caliper Technologies Corp. | Use of nernstein voltage sensitive dyes in measuring transmembrane voltage |
| US6287758B1 (en) * | 2000-03-23 | 2001-09-11 | Axiom Biotechnologies, Inc. | Methods of registering trans-membrane electric potentials |
| US6852504B2 (en) * | 2001-08-08 | 2005-02-08 | Molecular Devices Corporation | Method for measuring cellular transmembrane potential changes |
-
2001
- 2001-08-08 US US09/924,797 patent/US6852504B2/en not_active Expired - Lifetime
-
2002
- 2002-08-07 EP EP02759289A patent/EP1421206B1/de not_active Expired - Lifetime
- 2002-08-07 CA CA2456115A patent/CA2456115C/en not_active Expired - Lifetime
- 2002-08-07 AT AT02759289T patent/ATE392484T1/de not_active IP Right Cessation
- 2002-08-07 DE DE60226151T patent/DE60226151T2/de not_active Expired - Lifetime
- 2002-08-07 WO PCT/US2002/025046 patent/WO2003014701A2/en not_active Ceased
-
2004
- 2004-12-22 US US11/020,536 patent/US7358064B2/en not_active Expired - Lifetime
Also Published As
| Publication number | Publication date |
|---|---|
| EP1421206B1 (de) | 2008-04-16 |
| CA2456115C (en) | 2011-07-19 |
| US6852504B2 (en) | 2005-02-08 |
| DE60226151D1 (de) | 2008-05-29 |
| EP1421206A2 (de) | 2004-05-26 |
| CA2456115A1 (en) | 2003-02-20 |
| WO2003014701A2 (en) | 2003-02-20 |
| US20030087332A1 (en) | 2003-05-08 |
| EP1421206A4 (de) | 2006-02-15 |
| WO2003014701A3 (en) | 2003-11-13 |
| DE60226151T2 (de) | 2009-05-28 |
| US20050153275A1 (en) | 2005-07-14 |
| US7358064B2 (en) | 2008-04-15 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| RER | Ceased as to paragraph 5 lit. 3 law introducing patent treaties |