ATE397079T1 - Verfahren zur klonierung mittels doppelter selektion und vektoren dafür - Google Patents

Verfahren zur klonierung mittels doppelter selektion und vektoren dafür

Info

Publication number
ATE397079T1
ATE397079T1 AT02740794T AT02740794T ATE397079T1 AT E397079 T1 ATE397079 T1 AT E397079T1 AT 02740794 T AT02740794 T AT 02740794T AT 02740794 T AT02740794 T AT 02740794T AT E397079 T1 ATE397079 T1 AT E397079T1
Authority
AT
Austria
Prior art keywords
cloning
double selection
vectors
vectors therefor
therefor
Prior art date
Application number
AT02740794T
Other languages
English (en)
Inventor
Fabien Bertaux
Florence Lagardette
Christine Lapize
Original Assignee
Genoway
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Genoway filed Critical Genoway
Application granted granted Critical
Publication of ATE397079T1 publication Critical patent/ATE397079T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/64—General methods for preparing the vector, for introducing it into the cell or for selecting the vector-containing host
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/65—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression using markers

Landscapes

  • Genetics & Genomics (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Electrotherapy Devices (AREA)
  • Radar Systems Or Details Thereof (AREA)
  • Inspection Of Paper Currency And Valuable Securities (AREA)
  • Sorting Of Articles (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
AT02740794T 2001-05-18 2002-05-17 Verfahren zur klonierung mittels doppelter selektion und vektoren dafür ATE397079T1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
FR0106568A FR2824844B1 (fr) 2001-05-18 2001-05-18 Procede de clonage par double selection et vecteurs pour ce procede

Publications (1)

Publication Number Publication Date
ATE397079T1 true ATE397079T1 (de) 2008-06-15

Family

ID=8863427

Family Applications (1)

Application Number Title Priority Date Filing Date
AT02740794T ATE397079T1 (de) 2001-05-18 2002-05-17 Verfahren zur klonierung mittels doppelter selektion und vektoren dafür

Country Status (7)

Country Link
US (1) US20040253688A1 (de)
EP (1) EP1390513B1 (de)
AT (1) ATE397079T1 (de)
CA (1) CA2447706A1 (de)
DE (1) DE60226859D1 (de)
FR (1) FR2824844B1 (de)
WO (1) WO2002095037A1 (de)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1507003B1 (de) 2002-05-22 2011-09-21 Riken Verfahren zur proteinherstellung in einem zellfreien proteinsynthesesystem unter verwendung eines thioredoxinfusionsprotein-expressionsvektors
WO2005087932A2 (en) * 2003-10-03 2005-09-22 Promega Corporation Vectors for directional cloning
US8293503B2 (en) 2003-10-03 2012-10-23 Promega Corporation Vectors for directional cloning
KR100681815B1 (ko) * 2005-12-02 2007-02-12 한국생명공학연구원 리컴비네이즈 매개 선택표지 유전자 발현유도에 의한 목표유전자 간편 클로닝 및 목표 단백질 신속 발현 방법.

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2646438B1 (fr) * 1989-03-20 2007-11-02 Pasteur Institut Procede de remplacement specifique d'une copie d'un gene present dans le genome receveur par l'integration d'un gene different de celui ou se fait l'integration
EP0426455B1 (de) * 1989-10-31 1995-09-06 Sagami Chemical Research Center Klonierungs-Plasmid-Vektor, Primer-Vektor davon und Herstellungsverfahren einer cDNA-Bank
EP0814165B1 (de) * 1996-06-21 2003-04-09 Suntory Limited Verfahren zur Herstellung von transformierten Hefezellen, die kein Selektionsmarker-Gen enthalten
US6090554A (en) * 1997-10-31 2000-07-18 Amgen, Inc. Efficient construction of gene targeting vectors
AU771534B2 (en) * 1999-05-05 2004-03-25 Phylogica Limited Isolating biological modulators from biodiverse gene fragment libraries
EP1196566B1 (de) * 1999-07-12 2006-02-01 Genentech, Inc. Expressionsvektoren und anwendungsmethoden
CA2388907A1 (en) * 1999-10-25 2001-05-03 Life Technologies Corporation Methods of manipulating and sequencing nucleic acid molecules using transposition and recombination
US20030211495A1 (en) * 2000-03-08 2003-11-13 Richard Hopkins Reverse n-hybrid screening method

Also Published As

Publication number Publication date
EP1390513B1 (de) 2008-05-28
DE60226859D1 (de) 2008-07-10
FR2824844B1 (fr) 2003-09-19
EP1390513A1 (de) 2004-02-25
US20040253688A1 (en) 2004-12-16
WO2002095037A1 (fr) 2002-11-28
FR2824844A1 (fr) 2002-11-22
CA2447706A1 (fr) 2002-11-28

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