ATE400648T1 - Transformation von bacillus, transformanten und mutanten-bibliotheken - Google Patents

Transformation von bacillus, transformanten und mutanten-bibliotheken

Info

Publication number
ATE400648T1
ATE400648T1 AT01957519T AT01957519T ATE400648T1 AT E400648 T1 ATE400648 T1 AT E400648T1 AT 01957519 T AT01957519 T AT 01957519T AT 01957519 T AT01957519 T AT 01957519T AT E400648 T1 ATE400648 T1 AT E400648T1
Authority
AT
Austria
Prior art keywords
transformants
bacillus
transformation
mutant libraries
constructs
Prior art date
Application number
AT01957519T
Other languages
English (en)
Inventor
Maria Diaz-Torres
Volker Schellenberger
Olga Selifonova
Thomas Morrison
Edwin Lee
Original Assignee
Genencor Int
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=22842887&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=ATE400648(T1) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Genencor Int filed Critical Genencor Int
Application granted granted Critical
Publication of ATE400648T1 publication Critical patent/ATE400648T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome

Landscapes

  • Genetics & Genomics (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Mycology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Saccharide Compounds (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Steroid Compounds (AREA)
AT01957519T 2000-08-11 2001-08-10 Transformation von bacillus, transformanten und mutanten-bibliotheken ATE400648T1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US22494800P 2000-08-11 2000-08-11

Publications (1)

Publication Number Publication Date
ATE400648T1 true ATE400648T1 (de) 2008-07-15

Family

ID=22842887

Family Applications (1)

Application Number Title Priority Date Filing Date
AT01957519T ATE400648T1 (de) 2000-08-11 2001-08-10 Transformation von bacillus, transformanten und mutanten-bibliotheken

Country Status (8)

Country Link
US (2) US20020182734A1 (de)
EP (1) EP1309677B2 (de)
AT (1) ATE400648T1 (de)
AU (1) AU2001279254A1 (de)
CA (1) CA2418317A1 (de)
DE (1) DE60134752D1 (de)
DK (1) DK1309677T4 (de)
WO (1) WO2002014490A2 (de)

Families Citing this family (124)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB0112226D0 (en) * 2001-05-18 2001-07-11 Danisco Method of improving dough and bread quality
US6936289B2 (en) 1995-06-07 2005-08-30 Danisco A/S Method of improving the properties of a flour dough, a flour dough improving composition and improved food products
ES2350891T3 (es) 1997-04-09 2011-01-28 Danisco A/S Lipasa y utilización de la misma para mejorar las masas y los productos horneados.
WO2000005396A1 (en) 1998-07-21 2000-02-03 Danisco A/S Foodstuff
US7498158B2 (en) 2001-05-15 2009-03-03 Novozymes A/S Alpha-amylase variant with altered properties
EP1387616B1 (de) 2001-05-18 2007-05-16 Danisco A/S Verfahren zur herstellung von teig mit einem enzym
EP2341070A3 (de) 2002-03-29 2011-10-19 Genencor International, Inc. Verstärkte Proteinexpression in Bacillus
AU2003223928A1 (en) * 2002-05-07 2003-11-11 Novozymes A/S Homologous recombination into bacterium for the generation of polynucleotide libraries
US7955814B2 (en) 2003-01-17 2011-06-07 Danisco A/S Method
DE602004030000D1 (de) 2003-01-17 2010-12-23 Danisco Verfahren zur in-situ-herstellung eines emulgators in einem nahrungsmittel
US20050196766A1 (en) 2003-12-24 2005-09-08 Soe Jorn B. Proteins
JP5244317B2 (ja) 2003-11-19 2013-07-24 ジェネンコー・インターナショナル・インク セリンプロテアーゼ、セリン酵素をコードする核酸、およびこれらを取り込んだベクターおよび宿主細胞
US7985569B2 (en) 2003-11-19 2011-07-26 Danisco Us Inc. Cellulomonas 69B4 serine protease variants
JP5697294B2 (ja) 2003-12-24 2015-04-08 デュポン ニュートリション バイオサイエンシーズ エーピーエス 糖脂質アシル基転移酵素変異体及びその製造方法
GB0716126D0 (en) * 2007-08-17 2007-09-26 Danisco Process
US7718408B2 (en) * 2003-12-24 2010-05-18 Danisco A/S Method
GB0405637D0 (en) 2004-03-12 2004-04-21 Danisco Protein
CN1938422A (zh) * 2004-04-08 2007-03-28 金克克国际有限公司 突变体α－淀粉酶
EP1776455B1 (de) 2004-07-16 2015-03-18 DuPont Nutrition Biosciences ApS Lipolytisches enzym, dessen anwendung in der lebensmittelindustrie
CA2597980C (en) 2005-02-18 2016-01-05 Genencor International, Inc. Polypeptides having alpha-amylase and granular starch hydrolyzing activity
AR055444A1 (es) 2005-10-12 2007-08-22 Procter & Gamble Uso y produccion de metalopretasa neutra estable al almacenamiento
WO2007085443A2 (en) 2006-01-24 2007-08-02 Purac Biochem Bv Genetic modification of homolactic thermophilic bacilli
US7968691B2 (en) 2006-08-23 2011-06-28 Danisco Us Inc. Pullulanase variants with increased productivity
US9447397B2 (en) 2006-10-10 2016-09-20 Danisco Us Inc. Glucoamylase variants with altered properties
DK2097531T3 (da) * 2006-12-21 2020-01-02 Novozymes Inc Fremgangsmåder til opnåelse af genetisk kompetence hos bacillus-celler
CN105950523A (zh) * 2006-12-21 2016-09-21 诺维信股份有限公司 在芽孢杆菌属细胞中获得遗传感受态的方法
CN101652474B (zh) 2007-01-25 2012-06-27 丹尼斯科有限公司 由地衣芽孢杆菌转化细胞制备脂酰基转移酶
BRPI0812446A2 (pt) 2007-06-06 2014-10-14 Danisco Us Inc Genecor Division Métodos para aperfeiçoamento de propriedades de proteína múltiplas
MX353478B (es) * 2007-07-31 2018-01-15 Bp Corp North America Inc Star Ensamblaje combinacional de sitios multiples, a la medida.
ES2504983T3 (es) 2007-10-09 2014-10-09 Danisco Us Inc. Variantes de glucoamilasa
US8592194B2 (en) 2007-10-09 2013-11-26 Danisco Us Inc. Glucoamylase variants with altered properties
EP2205732A2 (de) 2007-11-01 2010-07-14 Danisco US Inc. Herstellung von thermolysin und varianten davon sowie deren verwendung in flüssigreinigungsmitteln
BRPI0820500A2 (pt) 2007-11-05 2015-06-16 Danisco Us Inc Variantes de alfa-amilase de bacillus sp. Ts-23 com propriedades alteradas
KR20100085964A (ko) 2007-11-05 2010-07-29 다니스코 유에스 인크. 변경된 특성을 가진 알파-아밀라아제 변이체
DK2222842T3 (en) 2007-11-20 2015-01-19 Danisco Us Inc Glucoamylasevarianter with changed properties
BRPI0821253A2 (pt) 2007-12-21 2015-06-16 Danisco Método de degomagem, método de tratamento de uma fase de goma e uso de lipídio aciltransferase na degomagem com água de um óleo comestível
US8236545B2 (en) 2008-02-04 2012-08-07 Danisco Us Inc., Genencor Division TS23 alpha-amylase variants with altered properties
US9528114B2 (en) 2008-03-28 2016-12-27 Danisco Us Inc. Method for amplifying locus in bacterial cell
GB0807161D0 (en) * 2008-04-18 2008-05-21 Danisco Process
CN102057040A (zh) 2008-06-06 2011-05-11 丹尼斯科美国公司 具有改良特性的嗜热脂肪土芽孢杆菌α-淀粉酶(AMYS)变体
CN104694519B (zh) 2008-06-06 2023-10-27 丹尼斯科美国公司 包含微生物蛋白酶变体的组合物和方法
DK2337837T4 (en) 2008-09-25 2017-02-06 Danisco Us Inc ALPHA-AMYLASE MIXTURES AND PROCEDURES FOR USING IT
EP2362896A2 (de) 2008-11-11 2011-09-07 Danisco US Inc. Bacillus subtilisin mit mindestens einer kombinierbaren mutation
EP2362902B1 (de) 2008-11-11 2012-10-24 Danisco US, Inc., Genencor Division Zusammensetzungen und verfahren mit einer subtilisinvariante
BRPI0921827A2 (pt) 2008-11-11 2016-09-27 Danisco Us Inc composições e métodos compreendendo uma variante de subtilisina
MX339402B (es) 2008-11-11 2016-05-25 Danisco Us Inc Composiciones y metodos que comprenden variantes de serina proteasa.
CN102341495A (zh) 2009-03-10 2012-02-01 丹尼斯科美国公司 巨大芽孢杆菌菌株DSM90相关的α-淀粉酶及其使用方法
BRPI1013388A2 (pt) 2009-04-01 2019-04-09 Danisco Us Inc composição de limpeza que compreende uma alfa-amilase e uma protease e método de limpeza de um tecido ou superficie dura
CN102388131B (zh) 2009-04-08 2014-04-30 丹尼斯科美国公司 盐单胞菌属菌株WDG195相关α-淀粉酶及其使用方法
AR076941A1 (es) 2009-06-11 2011-07-20 Danisco Us Inc Cepa de bacillus para una mayor produccion de proteina
WO2011022465A1 (en) 2009-08-19 2011-02-24 Danisco Us Inc. Combinatorial variants of glucoamylase with improved specific activity and/or thermostability
CN107299123A (zh) 2009-08-19 2017-10-27 杜邦营养生物科学有限公司 葡糖淀粉酶的变体
EP2488638A1 (de) 2009-10-15 2012-08-22 DuPont Nutrition Biosciences ApS Acyltransferase-lipoproteine und herstellungsverfahren dafür
GB0920089D0 (en) 2009-11-17 2009-12-30 Danisco Method
EP2536832B1 (de) 2010-02-18 2015-02-25 Danisco US Inc. Amylase aus nesterenkonia und verfahren zu ihrer verwendung
US20120067373A1 (en) 2010-04-15 2012-03-22 Philip Frank Souter Automatic Dishwashing Detergent Composition
US20110281327A1 (en) 2010-04-15 2011-11-17 Danisco Us Inc. Compositions And Methods Comprising Variant Proteases
CN108410585A (zh) 2010-05-06 2018-08-17 宝洁公司 具有蛋白酶变体的消费品
CN103764823B (zh) 2011-05-05 2018-05-11 丹尼斯科美国公司 包含丝氨酸蛋白酶变体的组合物和方法
IN2014CN00650A (de) 2011-06-30 2015-04-03 Novozymes As
EP3572505A3 (de) 2011-10-28 2019-12-11 Danisco US Inc. Alpha-amylase-varianten zur bildung von varianter maltohexaosevariante
IN2014DN03298A (de) 2011-12-22 2015-06-26 Danisco Us Inc
US20150141316A1 (en) 2012-06-08 2015-05-21 Danisco Us Inc. Variant alpha amylases with enhanced activity on starch polymers
MX2015002099A (es) 2012-08-22 2015-05-11 Dupont Nutrition Biosci Aps Variantes que tienen actividad glucoamilasa.
EP3354728B1 (de) 2012-12-21 2020-04-22 Danisco US Inc. Alpha-amylase-varianten
CN110777132B (zh) 2013-03-11 2024-03-22 丹尼斯科美国公司 α-淀粉酶组合变体
US20160130571A1 (en) 2013-06-17 2016-05-12 Danisco Us Inc. Alpha-Amylase from Bacillaceae Family Member
US20160186102A1 (en) 2013-10-03 2016-06-30 Danisco Us Inc. Alpha-amylases from exiguobacterium, and methods of use, thereof
US20160160199A1 (en) 2013-10-03 2016-06-09 Danisco Us Inc. Alpha-amylases from exiguobacterium, and methods of use, thereof
US10358657B2 (en) 2013-12-20 2019-07-23 Deinove IS-targeting system for gene insertion and genetic engineering in Deinococcus bacteria
EP3098310B1 (de) * 2014-01-21 2019-01-16 Synplogen Co., Ltd. Verfahren zur vorbereitung von dna-einheit-zusammensetzung und verfahren zur erzeugung von verketteter dna
US9416393B2 (en) 2014-10-13 2016-08-16 American Sterilizer Company Biological indicator
MX2017014595A (es) 2015-05-22 2018-03-21 Dupont Nutrition Biosci Aps Acetolactato-descarboxilasa.
US20180142228A1 (en) 2015-05-22 2018-05-24 Dupont Nutrition Biosciences Aps Aldc production methods
MX380958B (es) 2016-01-25 2025-03-12 American Sterilizer Co Condensador para detectar microorganismos viables.
EP4095152A3 (de) 2016-03-04 2022-12-28 Danisco US Inc. Manipulierte ribosomale promotoren zur proteinerzeugung in mikroorganismen
CN105861405A (zh) * 2016-05-06 2016-08-17 中国科学院上海高等研究院 一种高转化率的枯草芽孢杆菌及其构建方法
DK3512941T3 (da) 2016-09-16 2021-06-28 Dupont Nutrition Biosci Aps Acetolactatdecarboxylasevarianter med forbedret specifik aktivitet
MX2019007335A (es) 2016-12-21 2019-09-09 Dupont Nutrition Biosci Aps Métodos para usar serina proteasas termoestables.
US20190367932A1 (en) 2017-01-23 2019-12-05 Danisco Us Inc. Methods and compositions for obtaining natural comptence in bacillus host cells
FI3585910T3 (fi) 2017-02-24 2024-06-19 Danisco Us Inc Koostumukset ja menetelmät proteiinin tuotannon lisäämiseksi bacillus licheniformiksessa
MX386582B (es) 2017-03-15 2025-03-19 Int N&H Denmark Aps Métodos para usar una serina proteasa de arqueas.
WO2018169750A1 (en) 2017-03-15 2018-09-20 Danisco Us Inc Trypsin-like serine proteases and uses thereof
US20200015499A1 (en) 2017-03-15 2020-01-16 Dupont Nutrition Biosciences Aps Trypsin-like serine proteases and uses thereof
EP3607097B1 (de) 2017-04-07 2023-06-21 DuPont Nutrition Biosciences ApS Bacillus-wirtszellen, welche beta-galactosidasen und lactasen in abwesenheit von p-nitrobenzylesterase nebenaktivität produzieren
US10876144B2 (en) 2017-07-14 2020-12-29 American Sterilizer Company Process for determining viability of test microorganisms of biological indicator and sterilization detection device for determining same
US10900062B2 (en) 2017-07-14 2021-01-26 American Sterilizer Company Process for determining viability of test microorganisms of biological indicator and sterilization detection device for determining same
US10889848B2 (en) 2017-07-14 2021-01-12 American Sterilizer Company Process for determining viability of test microorganisms of biological indicator and sterilization detection device for determining same
EP3655537A1 (de) 2017-08-23 2020-05-27 Danisco US Inc. Verfahren und zusammensetzungen zur effizienten genetischen modifikation von bacillus-licheniformis-stämmen
WO2019055261A1 (en) 2017-09-13 2019-03-21 Danisco Us Inc MODIFIED 5 'NON-TRANSLATED REGION (UTR) SEQUENCES FOR INCREASED PROTEIN PRODUCTION IN BACILLUS
EP3707253A1 (de) 2017-12-15 2020-09-16 Danisco US Inc. Cas9-varianten und verwendungsverfahren
WO2019135868A1 (en) 2018-01-03 2019-07-11 Danisco Us Inc Mutant and genetically modified bacillus cells and methods thereof for increased protein production
CN112654703A (zh) 2018-07-06 2021-04-13 杜邦营养生物科学有限公司 用于基于谷类的动物饲料的含木聚糖酶饲料添加剂
US11781147B2 (en) 2018-11-28 2023-10-10 Danisco Us Inc. Promoter sequences and methods thereof for enhanced protein production in Bacillus cells
EP3947656A1 (de) 2019-04-05 2022-02-09 Danisco US Inc. Verfahren zur integration von polynukleotiden in das genom von bacillus unter verwendung von zwei zirkulären rekombinanten dna-konstrukten und zusammensetzungen davon
CA3136114A1 (en) 2019-04-05 2020-10-08 Danisco Us Inc. Methods for integrating a donor dna sequence into the genome of bacillus using linear recombinant dna constructs and compositions thereof
JP7676362B2 (ja) 2019-08-14 2025-05-14 ダニスコ・ユーエス・インク バチルス・リケニフォルミス（ｂａｃｉｌｌｕｓ ｌｉｃｈｅｎｉｆｏｒｍｉｓ）における増加したタンパク質産生のための組成物及び方法
MX2022002712A (es) 2019-09-13 2022-08-10 Danisco Us Inc Variantes termoestables de glucosa isomerasa.
CA3155372A1 (en) 2019-10-28 2021-05-06 Sharief Barends Microbial host cells for the production of heterologous cyanuric acid hydrolases and biuret hydrolases
EP4058553A1 (de) 2019-11-11 2022-09-21 Danisco US Inc. Zusammensetzungen und verfahren zur verbesserten proteinproduktion in bacilluszellen
WO2021101950A1 (en) 2019-11-19 2021-05-27 Danisco Us Inc Selection marker free methods for modifying the genome of bacillus and compositions thereof
WO2021146411A1 (en) 2020-01-15 2021-07-22 Danisco Us Inc Compositions and methods for enhanced protein production in bacillus licheniformis
CN116897160A (zh) 2021-02-22 2023-10-17 丹尼斯科美国公司 在色素缺陷型芽孢杆菌属细胞中产生目的蛋白的方法和组合物
EP4347812A1 (de) 2021-05-24 2024-04-10 Danisco US Inc. Zusammensetzungen und verfahren zur verbesserten proteinproduktion in bacillus-zellen
WO2023274923A1 (en) 2021-06-30 2023-01-05 Henkel Ag & Co. Kgaa Composition with improved moisture management performance
US20240294888A1 (en) 2021-06-30 2024-09-05 Danisco Us Inc. Variant enzymes and uses thereof
EP4363542A1 (de) 2021-06-30 2024-05-08 Henkel AG & Co. KGaA Reinigungszusammensetzung mit lipolytischem enzym mit polyesteraseaktivität
WO2023274925A1 (en) 2021-06-30 2023-01-05 Henkel Ag & Co. Kgaa Cleaning composition with improved anti-gray performance and/or anti-pilling performance
CN117836315A (zh) 2021-08-20 2024-04-05 丹尼斯科美国公司 用于增强芽孢杆菌属细胞中蛋白质产生的方法和组合物
JP2024543466A (ja) 2021-11-16 2024-11-21 ダニスコ・ユーエス・インク バチルス属（ｂａｃｉｌｌｕｓ）細胞における強化されたタンパク質産生のための組成物及び方法
EP4499857A1 (de) 2022-03-24 2025-02-05 Danisco US Inc. Zusammensetzungen und verfahren zur herstellung und gewinnung von lektinen im industriellen massstab
US20250223623A1 (en) 2022-04-01 2025-07-10 Danisco Us Inc. Pro-region mutations enhancing protein production in gram-positive bacterial cells
CN114990035A (zh) * 2022-04-08 2022-09-02 华南农业大学 一株枯草芽孢杆菌bs168感受态工程菌株及其应用
WO2024040043A1 (en) 2022-08-16 2024-02-22 International N&H Denmark Aps Expression systems for phosphatases
KR20250054813A (ko) 2022-09-02 2025-04-23 다니스코 유에스 인크. 그람 양성 세포에서 단백질 생산을 향상시키는 신규한 프로모터 및 5'-비번역 영역 돌연변이
EP4590319A1 (de) 2022-09-20 2025-07-30 International N&H Denmark ApS Variante antivirale polypeptide
KR20250094671A (ko) 2022-10-24 2025-06-25 다니스코 유에스 인크. 바실러스 세포에서 단백질 생산을 향상시키기 위한 조성물 및 방법
WO2024118881A1 (en) 2022-12-01 2024-06-06 Genencor International Bv Iterative muliplex genome engineering in microbial cells using a bidirectional selection marker system
EP4627076A1 (de) 2022-12-01 2025-10-08 Danisco Us Inc Iterative multiplex-genommanipulation in mikrobiellen zellen unter verwendung eines rekombinanten selbsterregbaren selektionsmarkersystems
WO2024118882A1 (en) 2022-12-01 2024-06-06 Genencor International Bv Iterative multiplex genome engineering in microbial cells using a selection marker swapping system
WO2025007098A1 (en) 2023-06-30 2025-01-02 Danisco Us Inc. Recombinant fungal cells and methods thereof for industrial scale production of lectins
WO2025034713A2 (en) 2023-08-09 2025-02-13 Danisco Us Inc. Compositions and methods for enhanced protein production in gram‑positive bacterial cells
WO2025101486A1 (en) 2023-11-09 2025-05-15 Danisco Us Inc. Methods and compositions for enhanced protein production in bacillus cells
WO2026030345A1 (en) 2024-07-29 2026-02-05 Danisco Us Inc. Signal and pro-region sequence variants for enhanced protease production in bacillus cells

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5624829A (en) * 1984-07-03 1997-04-29 Gist-Brocades, B.V. Transformed industrial bacillus strains and methods for making and using them
US4828994A (en) * 1984-09-21 1989-05-09 Genex Corporation Bacillus strains with reduced extracellular protease levels
AU4431196A (en) * 1995-01-23 1996-08-14 Novo Nordisk A/S Dna integration by transposition
US5827515A (en) * 1995-08-30 1998-10-27 Sandoz Ltd. Bacillus thuringiensis sporulation gene
US5756316A (en) † 1995-11-02 1998-05-26 Genencor International, Inc. Molecular cloning by multimerization of plasmids
US6326204B1 (en) * 1997-01-17 2001-12-04 Maxygen, Inc. Evolution of whole cells and organisms by recursive sequence recombination

Also Published As

Publication number Publication date
US20020182734A1 (en) 2002-12-05
WO2002014490A3 (en) 2003-02-06
DE60134752D1 (de) 2008-08-21
EP1309677B2 (de) 2012-04-11
DK1309677T3 (da) 2008-11-03
WO2002014490A2 (en) 2002-02-21
AU2001279254A1 (en) 2002-02-25
DK1309677T4 (da) 2012-06-25
EP1309677B1 (de) 2008-07-09
CA2418317A1 (en) 2002-02-21
EP1309677A2 (de) 2003-05-14
US20060199222A1 (en) 2006-09-07

Similar Documents

Publication Publication Date Title
ATE400648T1 (de) Transformation von bacillus, transformanten und mutanten-bibliotheken
MY127434A (en) Method for producing substance utilizing microorganism
DE60132498D1 (en) Isoprenoidproduktion
WO2004070013A3 (en) Use of islet 1 as a marker for isolating or generating stem cells
BRPI0415774A (pt) cepa de microorganismos e processos de preparação de cepas otimizadas e de produção de uma molécula de interesse
EA200700329A1 (ru) Биохимический синтез 1,4-бутандиамина
DE69914954D1 (de) Aus substrathaltigen gasgemischen aethanol erzeugende clostridium stamme
AU2002255298A1 (en) Hydrogen-oxygen gas generator and method of generating hydrogen-oxygen gas using the generator
MX9702724A (es) Lipasa, microorganismo que la produce, procedimiento para la preparacion de dicha lipasa y su uso.
IL148642A0 (en) Medium for the protein-free and serum-free cultivation of cells
ES2193280T3 (es) Las enzimas, su preparacion y uso en la produccion de acrilato de amonio.
EP1154020A3 (de) Arginine Repressor deficienter Coryneform Bacteriumstamm und Verfahren zur Herstellung von L-Arginine
BR0317994A (pt) Cepas de levedura recombinante que produzem a fermentação de xilose
DE60042209D1 (de) Enzymatische synthese von deoxyribonucleotiden
DE60134163D1 (de) Nicht-pathogene mikroorganismen
AU2001263269A1 (en) Long wavelength engineered fluorescent proteins
NO20061470L (no) Fremgangsmate for fremstilling av gamma-karboksylerte proteiner
EP1370299A4 (de) Nukleinsäurekonstrukte, damit transformierte zellen und diese verwendende verfahren zur induzierung der leberregeneration und linderung von pfortaderhochdruck
SI1456238T1 (sl) Proizvodnja f(ab)2 fragmentov v sesalskih celicah
NZ508404A (en) Alcoholic fermentation using a mineral-enriched yeast as a nutrient source
WO1997047740A3 (en) VACCINE CONTAINING A PEROXIREDOXIN AND/OR A β-TUBULIN
PL331942A1 (en) Method of biologically transforming colchicinoid compounds into their correcponding 3-glycoside derivatives
NZ330464A (en) Methods for the preparation of biphenyl isoxazole sulfonamides
MY122370A (en) Rhodococcus microorganisms and process for producing amide compounds.
AU2002211843A1 (en) 2.5-diketo-d-gluconic acid reductases and methods of use

Legal Events

Date Code Title Description
RER Ceased as to paragraph 5 lit. 3 law introducing patent treaties