ATE401421T1 - Verfahren zum nachweis von dna methylierung - Google Patents
Verfahren zum nachweis von dna methylierungInfo
- Publication number
- ATE401421T1 ATE401421T1 AT03744855T AT03744855T ATE401421T1 AT E401421 T1 ATE401421 T1 AT E401421T1 AT 03744855 T AT03744855 T AT 03744855T AT 03744855 T AT03744855 T AT 03744855T AT E401421 T1 ATE401421 T1 AT E401421T1
- Authority
- AT
- Austria
- Prior art keywords
- dna methylation
- detecting dna
- nucleic acid
- acid molecules
- methylation pattern
- Prior art date
Links
- 238000000034 method Methods 0.000 title abstract 2
- 230000007067 DNA methylation Effects 0.000 title 1
- 230000003321 amplification Effects 0.000 abstract 2
- 230000011987 methylation Effects 0.000 abstract 2
- 238000007069 methylation reaction Methods 0.000 abstract 2
- 238000003199 nucleic acid amplification method Methods 0.000 abstract 2
- 150000007523 nucleic acids Chemical class 0.000 abstract 2
- 102000039446 nucleic acids Human genes 0.000 abstract 2
- 108020004707 nucleic acids Proteins 0.000 abstract 2
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L7/00—Heating or cooling apparatus; Heat insulating devices
- B01L7/52—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2400/00—Moving or stopping fluids
- B01L2400/04—Moving fluids with specific forces or mechanical means
- B01L2400/0475—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure
- B01L2400/0487—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure fluid pressure, pneumatics
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Biochemistry (AREA)
- Wood Science & Technology (AREA)
- General Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Clinical Laboratory Science (AREA)
- Biotechnology (AREA)
- Immunology (AREA)
- Analytical Chemistry (AREA)
- Physics & Mathematics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Biophysics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Saccharide Compounds (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DE10214232A DE10214232A1 (de) | 2002-03-25 | 2002-03-25 | Verfahren und Vorrichtung für die DNA Methylierungsanalyse |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ATE401421T1 true ATE401421T1 (de) | 2008-08-15 |
Family
ID=28050981
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AT03744855T ATE401421T1 (de) | 2002-03-25 | 2003-03-25 | Verfahren zum nachweis von dna methylierung |
Country Status (7)
| Country | Link |
|---|---|
| US (1) | US20050153296A1 (de) |
| EP (1) | EP1488008B1 (de) |
| JP (1) | JP2005520556A (de) |
| AT (1) | ATE401421T1 (de) |
| AU (1) | AU2003226710A1 (de) |
| DE (2) | DE10214232A1 (de) |
| WO (1) | WO2003080862A1 (de) |
Families Citing this family (22)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE10346363B4 (de) * | 2003-09-30 | 2005-09-29 | Epigenomics Ag | Verfahren zur Methylierungsanalyse von DNA |
| EP1568786A3 (de) * | 2004-02-13 | 2007-08-29 | Affymetrix, Inc. (A US Entity) | Analyse der Methylierung mittels Nukleinsäurearrays |
| WO2005090607A1 (en) | 2004-03-08 | 2005-09-29 | Rubicon Genomics, Inc. | Methods and compositions for generating and amplifying dna libraries for sensitive detection and analysis of dna methylation |
| US7449297B2 (en) * | 2005-04-14 | 2008-11-11 | Euclid Diagnostics Llc | Methods of copying the methylation pattern of DNA during isothermal amplification and microarrays |
| US7820385B2 (en) * | 2006-03-22 | 2010-10-26 | The United States Of America As Represented By The Department Of Health And Human Services, Centers For Disease Control And Prevention | Method for retaining methylation pattern in globally amplified DNA |
| US7901882B2 (en) | 2006-03-31 | 2011-03-08 | Affymetrix, Inc. | Analysis of methylation using nucleic acid arrays |
| US20080003609A1 (en) * | 2006-05-10 | 2008-01-03 | The Cleveland Clinic Foundation | Method of detecting bladder urothelial carcinoma |
| US20080213870A1 (en) * | 2007-03-01 | 2008-09-04 | Sean Wuxiong Cao | Methods for obtaining modified DNA from a biological specimen |
| WO2009006543A1 (en) | 2007-07-02 | 2009-01-08 | Euclid Diagnostics Llc | Methods for evaluating the methylation status of a polynucleotide |
| GB0714058D0 (en) * | 2007-07-19 | 2007-08-29 | Abertec Ltd | detection of methylation in nucleic acid sequences |
| WO2010037001A2 (en) | 2008-09-26 | 2010-04-01 | Immune Disease Institute, Inc. | Selective oxidation of 5-methylcytosine by tet-family proteins |
| US12527665B2 (en) | 2008-12-11 | 2026-01-20 | Pacific Biosciences Of California, Inc. | Methods for identifying modified bases in nucleic acid templates |
| US9175338B2 (en) | 2008-12-11 | 2015-11-03 | Pacific Biosciences Of California, Inc. | Methods for identifying nucleic acid modifications |
| EP2309005B1 (de) * | 2009-08-03 | 2015-03-04 | Epigenomics AG | Verfahren zur Konservierung von genomischer DNA-Sequenzkomplexität |
| US8795592B2 (en) * | 2010-09-23 | 2014-08-05 | Analogic Corporation | Sample thermal cycling |
| ES3018861T3 (en) * | 2011-12-13 | 2025-05-19 | Univ Oslo Hf | Method for detection of hydroxymethylation status |
| US11130991B2 (en) * | 2017-03-08 | 2021-09-28 | The University Of Chicago | Method for highly sensitive DNA methylation analysis |
| CN110741092A (zh) * | 2017-03-08 | 2020-01-31 | 哈佛学院董事及会员团体 | 扩增dna以维持甲基化状态的方法 |
| JP2020130047A (ja) * | 2019-02-19 | 2020-08-31 | 学校法人幾徳学園 | メチル化酵素及びそれを用いたメチル化解析方法 |
| JP6761889B1 (ja) | 2019-11-11 | 2020-09-30 | 株式会社Ｇｓｐエンタープライズ | 抗ヒトＣｒｉｐｔｏ−１抗体 |
| AU2021319150B2 (en) | 2020-07-30 | 2025-09-25 | Biomodal Limited | Compositions and methods for nucleic acid analysis |
| WO2024249200A1 (en) * | 2023-05-26 | 2024-12-05 | Illumina, Inc. | Methods for preserving methylation status during clustering |
Family Cites Families (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE3379659D1 (en) * | 1983-07-29 | 1989-05-24 | Henning Berlin Gmbh | Preactivated surfaces of synthetic materials for immobilizing organochemical and biological substances, process for preparing them and their use |
| GB9104453D0 (en) * | 1991-03-02 | 1991-04-17 | Tepnel Medical Ltd | Improvements relating to enzymes |
| US6251594B1 (en) * | 1997-06-09 | 2001-06-26 | Usc/Norris Comprehensive Cancer Ctr. | Cancer diagnostic method based upon DNA methylation differences |
| AU9660698A (en) * | 1997-08-29 | 1999-03-16 | Osvaldo J. Lopez | Dna methyltransferase genotyping |
| US5912129A (en) * | 1998-03-05 | 1999-06-15 | Vinayagamoorthy; Thuraiayah | Multi-zone polymerase/ligase chain reaction |
| US6312926B1 (en) * | 1998-08-14 | 2001-11-06 | University Of Medicine & Dentistry Of New Jersey | mRNA capping enzymes and uses thereof |
| IL154663A0 (en) * | 2000-09-01 | 2003-09-17 | Epigenomics Ag | Method for determining the degree of methylation of defined cytosines in genomic dna in the sequence context 5'-cpg-3' |
| EP1488003A1 (de) * | 2002-03-25 | 2004-12-22 | Epigenomics AG | Verfahren zur bestimmung des methylierungsmusters von nukleinsäuren mit hilfe von massenspektrometern |
-
2002
- 2002-03-25 DE DE10214232A patent/DE10214232A1/de not_active Ceased
-
2003
- 2003-03-25 WO PCT/EP2003/003104 patent/WO2003080862A1/en not_active Ceased
- 2003-03-25 AU AU2003226710A patent/AU2003226710A1/en not_active Abandoned
- 2003-03-25 EP EP03744855A patent/EP1488008B1/de not_active Expired - Lifetime
- 2003-03-25 AT AT03744855T patent/ATE401421T1/de not_active IP Right Cessation
- 2003-03-25 US US10/509,144 patent/US20050153296A1/en not_active Abandoned
- 2003-03-25 DE DE60322195T patent/DE60322195D1/de not_active Expired - Lifetime
- 2003-03-25 JP JP2003578586A patent/JP2005520556A/ja active Pending
Also Published As
| Publication number | Publication date |
|---|---|
| JP2005520556A (ja) | 2005-07-14 |
| EP1488008B1 (de) | 2008-07-16 |
| WO2003080862A1 (en) | 2003-10-02 |
| DE60322195D1 (de) | 2008-08-28 |
| US20050153296A1 (en) | 2005-07-14 |
| AU2003226710A1 (en) | 2003-10-08 |
| EP1488008A1 (de) | 2004-12-22 |
| DE10214232A1 (de) | 2003-10-23 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| RER | Ceased as to paragraph 5 lit. 3 law introducing patent treaties |