ATE402263T1 - Zusammensetzungen und verfahren zur protein- sekretion - Google Patents

Zusammensetzungen und verfahren zur protein- sekretion

Info

Publication number
ATE402263T1
ATE402263T1 AT99911542T AT99911542T ATE402263T1 AT E402263 T1 ATE402263 T1 AT E402263T1 AT 99911542 T AT99911542 T AT 99911542T AT 99911542 T AT99911542 T AT 99911542T AT E402263 T1 ATE402263 T1 AT E402263T1
Authority
AT
Austria
Prior art keywords
proteins
compositions
methods
functional
interest
Prior art date
Application number
AT99911542T
Other languages
English (en)
Inventor
Joel Weiner
Raymond Turner
Original Assignee
Univ Alberta
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Alberta filed Critical Univ Alberta
Application granted granted Critical
Publication of ATE402263T1 publication Critical patent/ATE402263T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/24—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Enterobacteriaceae (F), e.g. Citrobacter, Serratia, Proteus, Providencia, Morganella, Yersinia
    • C07K14/245—Escherichia (G)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C12N15/625—DNA sequences coding for fusion proteins containing a sequence coding for a signal sequence
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/034—Fusion polypeptide containing a localisation/targetting motif containing a motif for targeting to the periplasmic space of Gram negative bacteria as a soluble protein, i.e. signal sequence should be cleaved
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/61—Fusion polypeptide containing an enzyme fusion for detection (lacZ, luciferase)

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
AT99911542T 1998-04-01 1999-03-29 Zusammensetzungen und verfahren zur protein- sekretion ATE402263T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US09/053,197 US6022952A (en) 1998-04-01 1998-04-01 Compositions and methods for protein secretion
US09/085,761 US6335178B1 (en) 1998-04-01 1998-05-28 Compositions and methods for protein secretion

Publications (1)

Publication Number Publication Date
ATE402263T1 true ATE402263T1 (de) 2008-08-15

Family

ID=21982559

Family Applications (1)

Application Number Title Priority Date Filing Date
AT99911542T ATE402263T1 (de) 1998-04-01 1999-03-29 Zusammensetzungen und verfahren zur protein- sekretion

Country Status (3)

Country Link
US (2) US6022952A (de)
AT (1) ATE402263T1 (de)
DE (1) DE69939163D1 (de)

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US7083945B1 (en) * 2000-10-27 2006-08-01 The Board Of Regents Of The University Of Texas System Isolation of binding proteins with high affinity to ligands
US20030180888A1 (en) * 2000-11-03 2003-09-25 Millennium Pharmaceuticals, Inc. CD2000 and CD2001 molecules, and uses thereof
WO2002064025A2 (en) 2001-02-15 2002-08-22 Mount Sinai School Of Medicine Of New York University Inhibition of atf2 activity to treat cancer
US7857972B2 (en) * 2003-09-05 2010-12-28 Foret Plasma Labs, Llc Apparatus for treating liquids with wave energy from an electrical arc
US7622693B2 (en) * 2001-07-16 2009-11-24 Foret Plasma Labs, Llc Plasma whirl reactor apparatus and methods of use
CN1561223A (zh) * 2001-08-24 2005-01-05 斯塔凡·斯特伦布拉德 新型药物
US20060270043A1 (en) * 2002-01-23 2006-11-30 Blattner Frederick R Bacteria with reduced genome
US8765408B2 (en) 2002-01-23 2014-07-01 Wisconsin Alumni Research Foundation Prophage element-free bacteria
US8039243B2 (en) * 2002-01-23 2011-10-18 Wisconsin Alumni Research Foundation Insertion sequence-free bacteria
US8119365B2 (en) * 2002-01-23 2012-02-21 Wisconsin Alumni Research Foundation Insertion sequence-free bacteria
US6989265B2 (en) * 2002-01-23 2006-01-24 Wisconsin Alumni Research Foundation Bacteria with reduced genome
AU2003218217A1 (en) * 2002-03-15 2003-09-29 The Trustees Of The University Of Pennsylvania Heterologous protein production using the twin arginine translocation pathway
EP1487966B1 (de) * 2002-03-23 2006-12-13 Research Development Foundation Sekretion von proteinen mit mehreren disulfidbindungen in bakterien und verwendungen davon
US7303906B2 (en) * 2002-09-06 2007-12-04 Wisconsin Alumni Research Foundation Competent bacteria
DE10256669B4 (de) 2002-12-04 2006-03-09 Universitätsklinikum Charité an der Humboldt-Universität zu Berlin Technologietransferstelle Gemisch mindestens zweier Fusionsproteine sowie ihre Herstellung und Verwendung
CA2560074A1 (en) * 2004-03-18 2005-11-03 Board Of Regents, The University Of Texas System Combinatorial protein library screening by periplasmic expression
JP4730302B2 (ja) * 2004-04-20 2011-07-20 味の素株式会社 タンパク質の製造法
EP1791961B1 (de) * 2004-08-18 2011-06-08 The Governors of the University of Alberta Proteinherstellungsverfahren unter nutzung von yebf
CA2683165C (en) * 2006-04-05 2013-06-11 Foret Plasma Labs, Llc System, method and apparatus for treating liquids with wave energy from plasma
WO2008051383A2 (en) * 2006-10-19 2008-05-02 Amgen Inc. Use of alcohol co-solvents to improve pegylation reaction yields
PE20081140A1 (es) * 2006-10-25 2008-09-22 Amgen Inc Agentes terapeuticos a base de peptidos derivados de toxinas
EP2162540A2 (de) 2007-05-22 2010-03-17 Amgen Inc. Zusammensetzungen und verfahren zur herstellung biologisch aktiver fusionsproteine
CA2755336C (en) 2009-03-20 2015-07-14 Amgen Inc. Carrier immunoglobulins and uses thereof
US20120231006A1 (en) 2009-11-20 2012-09-13 Amgen Inc. Anti-orai1 antigen binding proteins and uses thereof
AU2011289426A1 (en) 2010-08-10 2013-02-28 Amgen Inc. Dual function in vitro target binding assay for the detection of neutralizing antibodies against target antibodies
MX360946B (es) 2010-09-22 2018-10-29 Amgen Inc Star Inmunoglobulinas portadoras y usos de las mismas.
JP2014507136A (ja) 2011-01-18 2014-03-27 アムジエン・インコーポレーテツド ＮａＶ１．７ノックアウトマウスとそれらの使用
PH12013501865A1 (en) 2011-03-16 2014-01-06 Amgen Inc Potent and selective inhibitors of nav1.3 and nav1.7
JP6151704B2 (ja) 2011-10-20 2017-06-21 スカラブ ゲノミクス， エルエルシー 遺伝的安定性が向上したゲノム縮小化細菌
AU2013222188A1 (en) 2012-02-22 2014-09-11 Amgen Inc. Autologous mammalian models derived from induced pluripotent stem cells and related methods
WO2014165277A2 (en) 2013-03-12 2014-10-09 Amgen Inc. POTENT AND SELECTIVE INHIBITORS OF Nav1.7
WO2015031686A1 (en) 2013-08-30 2015-03-05 Amgen Inc. High titer recombinant aav vector production in adherent and suspension cells
WO2015073720A1 (en) 2013-11-14 2015-05-21 Scarab Genomics, Llc Bacteria with improved metabolic capacity
WO2016061141A1 (en) 2014-10-13 2016-04-21 University Of Maryland, Baltimore Methods for treating cardiovascular dysfunction and improving fluid homeostasis with a peptide hormone
MX377591B (es) 2014-10-15 2025-03-10 Amgen Inc Elementos promotores y reguladores para mejorar la expresión de genes heterólogos en células hospederas.
CN104830745B (zh) * 2015-04-28 2018-06-05 江南大学 一种生产γ-氨基丁酸的方法
JP6937309B2 (ja) 2016-01-27 2021-09-22 ジャスト−エヴォテック バイオロジックス、インコーポレイテッド ハイブリッドプロモーターおよびその使用
JP7116059B2 (ja) 2016-11-21 2022-08-09 ジャスト－エヴォテック バイオロジックス、インコーポレイテッド アフリベルセプト製剤及びその使用
EP3668985B1 (de) 2017-08-17 2021-06-16 Just-Evotec Biologics, Inc. Verfahren zur reinigung von glykosyliertem protein aus wirtszellen-galectinen und anderen verunreinigungen
MY202872A (en) 2017-11-20 2024-05-28 Just Evotec Biologics Inc Aflibercept formulations containing a lysine salt as tonicifying agent and uses thereof
JP2022520793A (ja) 2019-02-15 2022-04-01 ジャスト－エヴォテック バイオロジックス、インコーポレイテッド 自動バイオ製造システム、施設、及びプロセス
EP4370647A1 (de) 2021-07-15 2024-05-22 Just-Evotec Biologics, Inc. Bidirektionales perfusionssystem mit tangentialer strömungsfiltration (tff)
JP2025506122A (ja) 2022-02-10 2025-03-07 アムジエン・インコーポレーテツド ハイスループット配列決定のための抗体タンパク質産物発現コンストラクト
WO2024228717A2 (en) 2022-09-07 2024-11-07 Quantitative Biosciences, Inc. Fentanyl-specific single variable-domain antibodies and use in a continuous agglutination assay
WO2024226439A1 (en) 2023-04-24 2024-10-31 Quantitative Biosciences, Inc. Engineered biosensor strains of e. coli for continuous aerobic detection of analytes
WO2025128343A1 (en) 2023-12-11 2025-06-19 Just-Evotec Biologics, Inc. Protein expression using trans-splicing and split selectable markers
EP4711442A1 (de) 2024-09-15 2026-03-18 Quantitative Biosciences, Inc. Mikrofluidischer chip zur parallelen kultivierung mikrobieller biosensorstämme

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US4683195A (en) * 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
US4683202A (en) * 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences

Also Published As

Publication number Publication date
DE69939163D1 (de) 2008-09-04
US6335178B1 (en) 2002-01-01
US6022952A (en) 2000-02-08

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