ATE406459T1 - Entfernung von farbstoffmarkierten didesoxyterminatoren aus dna- sequenzierungsreaktionen. - Google Patents

Entfernung von farbstoffmarkierten didesoxyterminatoren aus dna- sequenzierungsreaktionen.

Info

Publication number
ATE406459T1
ATE406459T1 AT00970699T AT00970699T ATE406459T1 AT E406459 T1 ATE406459 T1 AT E406459T1 AT 00970699 T AT00970699 T AT 00970699T AT 00970699 T AT00970699 T AT 00970699T AT E406459 T1 ATE406459 T1 AT E406459T1
Authority
AT
Austria
Prior art keywords
didesoxyterminators
removal
dna sequencing
sequencing reactions
dye labeled
Prior art date
Application number
AT00970699T
Other languages
English (en)
Inventor
Douglas Spicer
Karin Hughes
Robert Kaiser
James Mahoney
Amy Springer
Mark Stolowitz
Carl Weissman
Original Assignee
Cambrex Bio Science Rockland I
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Cambrex Bio Science Rockland I filed Critical Cambrex Bio Science Rockland I
Application granted granted Critical
Publication of ATE406459T1 publication Critical patent/ATE406459T1/de

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869Methods for sequencing
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Biotechnology (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Immunology (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Electrostatic Separation (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
AT00970699T 1999-10-06 2000-10-06 Entfernung von farbstoffmarkierten didesoxyterminatoren aus dna- sequenzierungsreaktionen. ATE406459T1 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US15818899P 1999-10-06 1999-10-06
US16405099P 1999-11-08 1999-11-08
US09/564,117 US6414136B1 (en) 1999-10-06 2000-05-03 Removal of dye-labeled dideoxy terminators from DNA sequencing reactions

Publications (1)

Publication Number Publication Date
ATE406459T1 true ATE406459T1 (de) 2008-09-15

Family

ID=27388145

Family Applications (1)

Application Number Title Priority Date Filing Date
AT00970699T ATE406459T1 (de) 1999-10-06 2000-10-06 Entfernung von farbstoffmarkierten didesoxyterminatoren aus dna- sequenzierungsreaktionen.

Country Status (10)

Country Link
US (2) US6414136B1 (de)
EP (1) EP1240355B1 (de)
JP (1) JP2003511046A (de)
AT (1) ATE406459T1 (de)
AU (2) AU7872000A (de)
CA (1) CA2386820A1 (de)
DE (1) DE60040091D1 (de)
DK (1) DK1240355T3 (de)
ES (1) ES2310524T3 (de)
WO (2) WO2001025490A1 (de)

Families Citing this family (25)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6734401B2 (en) * 2000-06-28 2004-05-11 3M Innovative Properties Company Enhanced sample processing devices, systems and methods
ES2449445T3 (es) 2000-06-28 2014-03-19 3M Innovative Properties Co. Dispositivos, sistemas y métodos mejorados para el tratamiento de muestras
US20030228600A1 (en) * 2000-07-14 2003-12-11 Eppendorf 5 Prime, Inc. DNA isolation method and kit
US7075656B2 (en) * 2001-12-11 2006-07-11 Adc Telecommunications, Inc. Method and algorithm for continuous wavelength locking
US7192560B2 (en) * 2001-12-20 2007-03-20 3M Innovative Properties Company Methods and devices for removal of organic molecules from biological mixtures using anion exchange
US7347976B2 (en) * 2001-12-20 2008-03-25 3M Innovative Properties Company Methods and devices for removal of organic molecules from biological mixtures using a hydrophilic solid support in a hydrophobic matrix
US7981600B2 (en) 2003-04-17 2011-07-19 3M Innovative Properties Company Methods and devices for removal of organic molecules from biological mixtures using an anion exchange material that includes a polyoxyalkylene
US7939249B2 (en) 2003-12-24 2011-05-10 3M Innovative Properties Company Methods for nucleic acid isolation and kits using a microfluidic device and concentration step
US20060160122A1 (en) * 2004-02-18 2006-07-20 Applera Corporation Polyelectrolyte-coated size-exclusion ion-exchange particles
US20050196856A1 (en) * 2004-02-18 2005-09-08 Applera Corporation Polyelectrolyte-coated size-exclusion ion-exchange particles
US20050181378A1 (en) * 2004-02-18 2005-08-18 Applera Corporation Polyelectrolyte-coated size-exclusion ion-exchange particles
US7805228B2 (en) * 2004-08-19 2010-09-28 Spx Corporation Vehicle diagnostic device
FR2886735B1 (fr) * 2005-06-01 2015-09-11 Biomerieux Sa Procede de marquage ou de traitement d'un echantillon biologique contenant des molecules biologiques d'interet, notamment des acides nucleiques
CN101351260B (zh) * 2005-11-01 2011-12-21 西北大学 用于微通道分离的基质和动态聚合物体系和组合物
JP4919745B2 (ja) * 2006-09-13 2012-04-18 日産自動車株式会社 ナノ粒子及びナノ粒子複合体
US8128893B2 (en) 2006-12-22 2012-03-06 3M Innovative Properties Company Thermal transfer methods and structures for microfluidic systems
EP2107944B1 (de) * 2006-12-22 2013-03-27 3M Innovative Properties Company Verbesserte probenverarbeitungsvorrichtungen, -systeme und -verfahren
US20090246762A1 (en) 2008-03-31 2009-10-01 Applera Corporation, Applied Biosystems Group Nucleic acid terminators incorporating a cationic moiety and methods for their use
KR101992503B1 (ko) 2011-05-18 2019-06-24 디아소린 에스.피.에이. 샘플 처리 장치에서 선택된 부피의 물질의 존재를 검출하기 위한 시스템 및 방법
KR20140022399A (ko) 2011-05-18 2014-02-24 쓰리엠 이노베이티브 프로퍼티즈 컴파니 샘플 처리 장치 상의 체적 계량을 위한 시스템 및 방법
US9067205B2 (en) 2011-05-18 2015-06-30 3M Innovative Properties Company Systems and methods for valving on a sample processing device
WO2013048163A2 (en) * 2011-09-29 2013-04-04 Snu R&Db Foundation Convenient and efficient purification method for chemically labeled nucleic acids
US20170051344A1 (en) 2014-01-24 2017-02-23 Life Technologies Corporation Purification Chemistries and Formats for Sanger DNA Sequencing Reactions on a Micro-Fluidics Device
GB201700983D0 (en) * 2017-01-20 2017-03-08 Life Tech As Polymeric particles
KR20190028009A (ko) 2017-09-08 2019-03-18 서울대학교산학협력단 pH 제어에 의한 화학적으로 표지된 올리고 핵산의 간편하고 효율적인 분리정제법

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5512439A (en) 1988-11-21 1996-04-30 Dynal As Oligonucleotide-linked magnetic particles and uses thereof
WO1991011533A1 (en) 1990-01-26 1991-08-08 E.I. Du Pont De Nemours And Company Method for isolating primer extension products from template-directed dna polymerase reactions
US5281356A (en) * 1993-03-25 1994-01-25 Lever Brothers Company Heavy duty liquid detergent compositions containing non-proteolytic enzymes comprising capsules comprising proteolytic enzyme and composite polymer
US5564104A (en) 1993-06-08 1996-10-08 Cortex Biochem, Inc. Methods of removing radioactively labled biological molecules from liquid radioactive waste
US6103127A (en) 1993-06-08 2000-08-15 Cortex Biochem, Inc. Methods for removing hazardous organic molecules from liquid waste
US5827531A (en) 1994-12-02 1998-10-27 The United States Of America As Represented By The Administrator Of The National Aeronautics And Space Administration Microcapsules and methods for making
US5683875A (en) 1995-05-04 1997-11-04 Hewlett-Packard Company Method for detecting a target nucleic acid analyte in a sample
US5830912A (en) 1996-11-15 1998-11-03 Molecular Probes, Inc. Derivatives of 6,8-difluoro-7-hydroxycoumarin
US5935572A (en) 1997-01-10 1999-08-10 Collaborative Laboratories, Inc. Composition containing protease separate from glycosidase for removing nits in treating lice infestation

Also Published As

Publication number Publication date
EP1240355A1 (de) 2002-09-18
ES2310524T3 (es) 2009-01-16
WO2001025491A1 (en) 2001-04-12
EP1240355B1 (de) 2008-08-27
US6414136B1 (en) 2002-07-02
CA2386820A1 (en) 2001-04-12
AU8003900A (en) 2001-05-10
DE60040091D1 (de) 2008-10-09
JP2003511046A (ja) 2003-03-25
WO2001025490A1 (en) 2001-04-12
EP1240355A4 (de) 2004-12-08
US6818760B1 (en) 2004-11-16
AU7872000A (en) 2001-05-10
DK1240355T3 (da) 2008-12-08

Similar Documents

Publication Publication Date Title
ATE406459T1 (de) Entfernung von farbstoffmarkierten didesoxyterminatoren aus dna- sequenzierungsreaktionen.
ATE469699T1 (de) Manipulation von mikroteilchen in mikrofluiden systemen
CN111748607B (zh) 微胶囊组合物及方法
US6660480B2 (en) Method for analyzing nucleic acids by means of a substrate having a microchannel structure containing immobilized nucleic acid probes
DK1233830T3 (da) Hybridiseringstest til biomateriale i en biokanal
ATE419527T1 (de) Mikrovorrichtung zum screening von biomolekülen
EP1488006A2 (de) Mikrokapseln mit kontrollierter durchlässigkeit die ein nukleinsäureamplifizierungsreaktionsgemisch enthalten und deren benutzung als reaktionsgefäss für parallele reaktionen
DE69731939D1 (de) Isolierung von einzelsträngigen nukleinsäuren
WO2002002227A8 (en) Devices and methods for carrying out chemical reactions using photogenerated reagents
CA2330569A1 (en) Chemical processing microsystems, diffusion-mixed microreactors and methods for preparing and using same
ATE268735T1 (de) Herstellung von kombinatorischen arrays von materialen durch methoden der synthese in lösung
WO2001073126A3 (en) Diagnostic devices containing porous material
Zhou et al. Harnessing HetHydrogel: A Universal Platform to Dropletize Single‐Cell Multiomics
ATE487938T1 (de) Konzentration und aufreinigung von nukleinsäureproben
ATE357516T1 (de) Modulation molekularer wechselwirkungspositionen in rns und anderen biomolekülen
Lee et al. Quadruplex amplification of polymorphic STR loci in a Korean population
AU6862900A (en) Genetic screening methods
US5776684A (en) Method for staining biomolecules using a gelled matrix
DE602005026412D1 (de) Zweidimensionale strangform- und längenabhängige trennung von nukleinsäurefragmenten
JP2015020122A (ja) マイクロチャンバー
KR102060306B1 (ko) 저장, 보존 및 색인이 가능한 생화학 물질 운반체 및 이의 제조방법
ATE151175T1 (de) Trägermaterial zur simultanen bindung von nukleinsäuren und deren expressionsprodukten
WO2026024999A1 (en) Controlled evaporation and density equalization of hydrogel microbeads
Davis Development of a microfluidic device for DNA diagnostics
AYI et al. CHEAPER, MORE RAPID, PORTABLE AND

Legal Events

Date Code Title Description
RER Ceased as to paragraph 5 lit. 3 law introducing patent treaties